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Volume 14
Issue 3
10.3390/plants14030447
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Systematic Review

Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus

by
Pilsung Kang
1,†,
Sung Jin Kim
1,†,
Ha Ju Park
2,
Se Jong Han
1,3,
Il-Chan Kim
1,
Hyoungseok Lee
1,3,* and
Joung Han Yim
1,2,*
1
Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of Korea
2
CRYOTECH Inc. 2F-204, 71, Mieumsandan 5-ro 41beon-gil, Gangseo-gu, Busan 46744, Republic of Korea
3
Polar Science, University of Science and Technology, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2025, 14(3), 447; https://doi.org/10.3390/plants14030447 (registering DOI)
Submission received: 18 December 2024 / Revised: 30 January 2025 / Accepted: 31 January 2025 / Published: 3 February 2025
(This article belongs to the Special Issue Plant Conservation Science and Practice)
Review Reports Versions Notes

Abstract

The stable long-term preservation of plant cells is crucial for biopharmaceuticals and food security. Therefore, the long-term cryopreservation of plant cells using a cryoprotective agent (CPA) is a crucial area of study. However, research on low-toxicity CPAs remains limited. We analyzed 1643 abstracts related to plant-cryopreservation (PCP) research published from 1967 to May 2023, spanning 56 years, from academic citation databases, with the search conducted in May 2023. Grouping these abstracts by five-year intervals revealed an increase in PCP papers until 2015, followed by a decline in the 2020s. In order to confirm the declining trend, we performed text-mining analysis using the Latent Dirichlet Allocation (LDA) algorithm, which identifies underlying topics across diverse documents to aid decision-making and classified the abstracts into three distinct topics: Topic 1, “Seed bank”; Topic 2, “Physiology”; and Topic 3, “Cryopreservation protocol”. The decline, particularly in “Cryopreservation protocol” research, is an important observation in this study. At the same time, this decrease may be due to the limited scope of Topic 3. However, we expect improvements with the development of new CPAs. This expectation is based on numerous ongoing studies focused on developing new CPAs for the cryopreservation of various animal and medical cell lines, with particular attention on polysaccharides as components that could reduce the required concentrations of existing CPAs.
Keywords: plant cryopreservation; cryoprotective agent; plant tissue culture; exopolysaccharide; polar regions; text-mining; meta-analysis plant cryopreservation; cryoprotective agent; plant tissue culture; exopolysaccharide; polar regions; text-mining; meta-analysis

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MDPI and ACS Style

Kang, P.; Kim, S.J.; Park, H.J.; Han, S.J.; Kim, I.-C.; Lee, H.; Yim, J.H. Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus. Plants 2025, 14, 447. https://doi.org/10.3390/plants14030447

AMA Style

Kang P, Kim SJ, Park HJ, Han SJ, Kim I-C, Lee H, Yim JH. Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus. Plants. 2025; 14(3):447. https://doi.org/10.3390/plants14030447

Chicago/Turabian Style

Kang, Pilsung, Sung Jin Kim, Ha Ju Park, Se Jong Han, Il-Chan Kim, Hyoungseok Lee, and Joung Han Yim. 2025. "Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus" Plants 14, no. 3: 447. https://doi.org/10.3390/plants14030447

APA Style

Kang, P., Kim, S. J., Park, H. J., Han, S. J., Kim, I.-C., Lee, H., & Yim, J. H. (2025). Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus. Plants, 14(3), 447. https://doi.org/10.3390/plants14030447

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