Characterisation of Aspergillus fumigatus Endocytic Trafficking within Airway Epithelial Cells Using High-Resolution Automated Quantitative Confocal Microscopy
, David W. Denning 1, Nick D. Read 1,†, Paul Bowyer 1,* and Sara Gago 1,*Round 1
Reviewer 1 Report
The authors have responded well to all the issues that I raised, except to the first one: I requested a comparison between the manual and automated analysis to be given in terms of performance measures. The authors have now computed a non-defined quantity that referred to as "concordance" in Suppl Table 2. This measure is not only non-defined, it is also computed in various ways; otherwise it would become clear that the comparison between the manual and automated analysis is actually not as good as Suppl Table 2 now states.
The authors need to redo this and compute performance measures as accuracy, recall and precision in terms of a comparison to the manual analysis (as ground truth) based on true positives, false positives and fals negatives. (I suppose that true negatives are not defined in this setting.)
Author Response
Please see attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The authors have adequately addressed all of my comments.
Author Response
We thank the reviewer for the time spent reviewing our manuscript.
Round 2
Reviewer 1 Report
The authors have responded to my comments by removing some information from the manuscript about the validation of automatic versus manual counting. I am fine with this decision, but the authors have now included a new statement and do not want to show the corresponding data; instead, they added "(data not shwon)". I do not agree with this procedure. I suggest the authors are going to add the description and the two tables they provided me with in their rebuttal to the Supplementary Material of this manuscript. Once this has been realized, I deem the manuscript acceptable for publication.
Author Response
Please See attachment
Author Response File: Author Response.pdf
