Modulatory Effect of Limosilactobacillus fermentum grx08 on the Anti-Oxidative Stress Capacity of Liver, Heart, and Kidney in High-Fat Diet Rats
, Longfei Zhang 1,2, Xiaoxiao Liu 1,2, Yang Liu 1,2, Kaidong Mao 3, Guiqi Shen 3, Yunchao Wa 1,2, Dawei Chen 1,2, Yujun Huang 1,2, Xia Chen 1,2 and Ruixia Gu 1,2,*
Ok-Su Kim
Round 1
Reviewer 1 Report
This manuscript is an in-vivo study that lactobacillus can affect the control of systemic organ by high-fat diet. This is very interesting topic. And this manuscript is relatively well organized.
I recommend that the introduction part is needed to improve for this manuscript's purpose.
Material and methods, line 81, change the number: 5 groups--> 4 groups of high fat diet group
Figure, 3,4. The magnification for microscopic observation is missing. and a detailed explanation of figures is needed.
Discussion part, description was repeated and must be shortened or expressed differently.
Author Response
Dear reviewer:
Thank you for reviewing the manuscript and giving us your valuable comments. The answers to your questions are as follows.
Q: I recommend that the introduction part is needed to improve for this manuscript's purpose.
A: The introduction part had been partially corrected.
Q: Material and methods, line 81, change the number: 5 groups--> 4 groups of high fat diet group
A: This error had been corrected.
Q: Figure, 3,4. The magnification for microscopic observation is missing. and a detailed explanation of figures is needed.
A: The magnification for microscopic observation had been added in Figure 2 3,4.
Q: Discussion part, description was repeated and must be shortened or expressed differently.
A: The repetitive description had been shortened.
Thank you and best regards.
Yours sincerely,
Hengxian Qu
Reviewer 2 Report
Review Article: fermentation-1965703
I had the opportunity to review the manuscript entitled “Modulatory effect of Lactobacillus fermentum grx08 on the antioxidative stress capacity of liver, heart and kidney in high-fat diet rats”. In this study, the authors explore the modulatory effect of Lactobacillus fermentum grx08 on the anti-oxidative stress in the liver, heart and kidney of healthy male rats (Wistar) divided into 5 groups, out of 3 groups were administered with high-fat diets. Administration of Lactobacillus fermentum grx08 significantly reduced serum levels of markers of liver, heart and kidney damage. It also enhanced the activity of antioxidant enzymes such as glutathione peroxidase in the liver, heart and kidney, eliminating NO radicals and reducing malondialdehyde content. Moreover, Lactobacillus fermentum grx08 showed a better modulating effect on antioxidative stress in the kidney, followed by the liver, and a weaker modulating effect on the heart.
The present paper, in line with the topic of the journal, is of interest to the scientific community, but the abstract needs to be revised. Materials and methods need to be more comprehensive, especially analytical methods, conclusions need to be included (section 4), and finally there are many graphs (22) and only 1 figure in the text.
Below my major considerations line-by-line
L. 11-12: enter the total number of diets used in the experiment.
L. 24: insert "rats" in the keywords.
L. 38: what does the acronym NAFDL mean? Please specify.
L. 60-62: I suggest that authors clearly describe the purpose of the work, e.g. "The aim of this work/paper/job is ........."
L. 64: "1.1 Microorganism and preparation of oral samples"; the methods and equipment used must be explained in detail in this section.
L. 68: rewrite in correct form "1×109 CFU/mL".
L. 72: rewrite as "Thirty healthy male Wistar rats ..... ".
L. 96: "1.4 Blood samples handling" the methods and equipment used must be explained in detail in this section.
L. 114: "1.7 Statistical methods". Tukey's test is a "post hoc test", the authors should explain it in more detail in this section, furthermore, the acronym 'SEs' what does it stand for? Standard error of the mean? Please Specify!
I would like to ask the authors why they used a parametric test instead of a non-parametric test (e.g. the non-parametric equivalent of ANOVA).
L. 138-142: Lactobacillus fermentum grx08 heat-inactivated bacteria had the same effect but had no significant effect on Crea (P>0.05). (Figure 1) Lactobacillus fermentum grx08 had the potential to enhance the resistance to the antioxidative stress capacity of the liver, heart and kidney in rats.
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L. 240-248: Specify the meaning of the acronyms 'NO' (Nitric oxide synthases), 'MDA' (Malondialdehyde), 'SOD' (Superoxide dismutase), 'GSH-Px' (Glutathione peroxidase).
L. 275-276: insert “section 4. Conclusion".
Author Response
Dear reviewer:
Thank you for reviewing the manuscript and giving us your valuable comments. The answers to your questions are as follows.
Q: L. 11-12: enter the total number of diets used in the experiment.
A: The total number of diets had been added.
Q: L. 24: insert "rats" in the keywords.
A: The keyword "rats" had been added.
Q: L. 38: what does the acronym NAFDL mean? Please specify.
A: NAFLD is an abbreviation for non-alcoholic fatty liver disease, and had been added to the text.
Q: L. 60-62: I suggest that authors clearly describe the purpose of the work, e.g. "The aim of this work/paper/job is ........."
A: The purpose of this work had been more clearly described.
Q: L. 64: "1.1 Microorganism and preparation of oral samples"; the methods and equipment used must be explained in detail in this section.
A: The methods and equipment used had been explained in detail.
Q: L. 68: rewrite in correct form "1×109CFU/mL".
A: This had been revised.
Q: L. 72: rewrite as "Thirty healthy male Wistar rats ..... ".
A: This had been revised.
Q: L. 96: "1.4 Blood samples handling" the methods and equipment used must be explained in detail in this section.
A: The methods and equipment used had been explained in detail.
Q: L. 114: "1.7 Statistical methods". Tukey's test is a "post hoc test", the authors should explain it in more detail in this section, furthermore, the acronym 'SEs' what does it stand for? Standard error of the mean? Please Specify!
I would like to ask the authors why they used a parametric test instead of a non-parametric test (e.g. the non-parametric equivalent of ANOVA).
A: First of all, there is an error here that the results were presented as the means ± SD (standard deviation) instead of means ± SE (standard error), which has been corrected in the text. The description of Tukey's test was also added. In addition, we consider that parametric tests are more efficient compared to nonparametric tests for our data information.
Q: L. 138-142: Lactobacillus fermentum grx08 heat-inactivated bacteria had the same effect but had no significant effect on Crea (P>0.05). (Figure 1) Lactobacillus fermentum grx08 had the potential to enhance the resistance to the antioxidative stress capacity of the liver, heart and kidney in rats.
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A: This error had been corrected.
Q: L. 240-248: Specify the meaning of the acronyms 'NO' (Nitric oxide synthases), 'MDA' (Malondialdehyde), 'SOD' (Superoxide dismutase), 'GSH-Px' (Glutathione peroxidase).
A: The meanings of these abbreviations had been added in the text where they first appear (line 119-120).
Q: L. 275-276: insert “section 4. Conclusion".
A: The “conclusion” had been inserted.
Thank you and best regards.
Yours sincerely,
Hengxian Qu
Round 2
Reviewer 2 Report
The paper has been greatly improved and deserves to be published in the Journal. I have only two small suggestions for the authors listed below:
Line 139: rewrite as "the increase of liver index (Table 1)."
Line 151: rewrite as "heart and kidney in rats (Figure 1)."
Author Response
Dear reviewer:
Thank you for reviewing the manuscript and giving us your valuable comments. The answers to your questions are as follows.
Q: Line 139: rewrite as "the increase of liver index (Table 1)."
A: This had been revised.
Q: Line 151: rewrite as "heart and kidney in rats (Figure 1)."
A: This had been revised.
Thank you and best regards.
Yours sincerely,
Hengxian Qu
