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Peer-Review Record

In Pursuit of Understanding the Rumen Microbiome

Fermentation 2023, 9(2), 114; https://doi.org/10.3390/fermentation9020114

by Rey Anthony Sanjorjo^1,†, Tenzin Tseten^1,†, Min-Kyoung Kang¹, Moonhyuk Kwon^2,*

and Seon-Won Kim^1,*

Reviewer 1: Anonymous

Reviewer 2:

Anusorn Cherdthong

Fermentation 2023, 9(2), 114; https://doi.org/10.3390/fermentation9020114

Submission received: 30 December 2022 / Revised: 20 January 2023 / Accepted: 23 January 2023 / Published: 25 January 2023

(This article belongs to the Special Issue Rumen Fermentation)

Round 1

Reviewer 1 Report

The author reviewed an understanding of the rumen microbiome, presented its components and its role in animal fermentation and nutrition. Then they introduced some major factors that might affect the rumen microbiome, trying to understand the effect of rumen microbiome in feed digestion and methane production. The authors did a good job in their literature review and very helpful for the introduction of rumen microbiome, while the present manuscript should undergo minor modification to make it in high-standard publication in Fermentation.

Minor comments:

1. There are some formats that need to be double-checked, e.g. The first letter of the first keyword shouldn’t be in bold. There is no in-text reference to Figure 4.

2. In the introduction, the author presented, that rumen microbiome is composed of anaerobic bacteria, protozoa, fungi, methanogenic archaea, and phages. However, no details are given of the content of phage in remen microbiome, or they affect the animal fermentation or products.

3. There are plenty factors that could change rumen microbiome, including diets, food additives, and host genetics, which have impacted on microbiome components. However, the early life interventions, like host weaning and weaning age, which influenced the establishment of rumen microbiome. In addition, the description of supplements in early-life interventions, was repeated in the parts of diet or feed additives, it’s better to integrate the part of early-life interventions to other parts.

4. Microbiota crosstalk with gut stem cell niche (https://doi.org/10.1002/imt2.54) will have a major impact on animal nutrition. Proper discussion in the text is recommended

Author Response

"Please see the attachment"

Jan 19^th, 2023

Re: Resubmission of manuscript “In pursuit of understanding the rumen microbiome”, Fermentation-2162771

Ms. Cathy Lu

Fermentation Journal

Respected Ms. Cathy Lu:

Thank you for the opportunity to revise our manuscript, “In pursuit of understanding the rumen microbiome”. We appreciate the careful review and constructive suggestions from the reviewers. It is our belief that the manuscript is substantially improved after making the suggested edits.

Following this letter are the editor and reviewer comments with our responses in blue color font, including how and where the text was modified. Changes made in the manuscript are using track change. The revision has been developed in consultation with all co-authors, and each author has given approval to the final form of this revision.

We very much hope the revised manuscript is accepted for publication in Journal.

Sincerely yours,

Seon-Won Kim, Ph. D.

Professor, Division of Applied Life Science

Gyeongsang National University

501, Jinju-Daero, Jinju-si, Gyeongsangnam-do, 52828, Republic of Korea

TEL: +82-55-772-1362, FAX: +82-55-759-9363

E-mail: [email protected]

Author Response File: Author Response.pdf

Reviewer 2 Report

Manuscript ID: fermentation-2162771
Type of manuscript: Review
Title: In pursuit of understanding the rumen microbiome
Authors: Rey Anthony Sanjorjo et al.

This article aims to provide a concise summary of rumen fermentation, microbial populations, and how different conditions impact the rumen microbiome. It is a fascinating study that demonstrates the advancement of current research on rumen ecology and contributes to the enhancement of ruminant output. There are obstacles for ruminant nutritionists to identify what dynamic is occurring in the rumen and how to manage it. I considered that if this study were to be published, it would be useful to readers interested in the extent of ruminant animals. I have the following comments:

Abstract:

-This section should be expanded beyond its current length. It does not cover what the content discusses. Additionally, any crucial number may be added to this section.

-Please include the purpose of this review.

Introduction

-L28-73: The authors begin by discussing methane production, while the title focuses on rumen microbe. It is unrelated to the issue in question. As you are aware, methane is just one of the end products produced by rumen microbes; thus, it is more pertinent, to begin with the main topic.

-L39-41: What are the distinctions between methanogenic archaea and anaerobic bacteria? Typically, while discussing microorganisms, three components are mentioned, such as bacteria, protozoa, and fungi.

-L47-49: There is evidence that 70-80% of ruminant energy comes from VFA. However, where protein supply was not discussed. How many proteins are produced by microorganisms? Discuss further!

-L53: According to Environmental Protection Agency EPA---According to Environmental Protection Agency (EPA)

-Figure 1 A: see comment above!

-Figure 2: The authors describe just two stages of rumen development, but information on adult animals is absent. Please expand.

-L147: See the previous remark. If archaea were considered, what about additional rumen bacteria species? It seems that the authors here concentrate solely on a few types of bacteria!

-Figure 3: There are several descriptions in the title figure, however, this title should be shortened, and more information must be provided in the main text. Please correct every figure!

-Conclusions

The current conclusion is very lengthy. It seems the authors want to debate more and more. This part should summarize the problem about which the authors want to tell the reader. In addition, this section should address the purpose of the current review. Please correct.