Tracking Reservoirs of Antimicrobial Resistance Genes in Environment

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Mechanism and Evolution of Antibiotic Resistance".

Deadline for manuscript submissions: 15 February 2025 | Viewed by 1367

Special Issue Editors


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Guest Editor
National Institute for Antibiotic Resistance and Infection Control, Ministry of Health, Tel Aviv, Israel
Interests: antibiotic resistance; environmental bacteria; lateral gene transfer; microbial evolution
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Guest Editor
Hydro-Chemistry and Water Research Center, Tel Aviv University, Tel Aviv-Yafo, Israel
Interests: identifying the fate and transport mechanisms of pharmaceuticals and degradation by-products in domestic; industrial and hospital wastewater; effluents and biosolids; industrial fish ponds, effluent irrigated fields, river streams and groundwater; technology development for water treatment and purification via Advanced Oxidation Processes (AOPs) for removal of pharmaceutical compounds from wastewater effluent
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Special Issue Information

Dear Colleagues,

Antimicrobial resistance (AMR) is a pressing global threat that necessitates immediate and concerted action. Beyond the clinical confines, AMR genes have insidiously permeated our environment, existing in soil, water systems, and beyond. The spread of these genes is propelled by a confluence of factors: agricultural practices, urban waste, and the very fabric of global travel. To effectively track and comprehend the reach of AMR, we rely on sophisticated molecular techniques and the precision of geographic information systems. Recognizing that AMR's ramifications transcend human health, it impacts animal welfare and the delicate balance of ecosystems, compelling us to adopt a holistic 'One Health' approach to tackle its multifaceted challenges. Swift action by policymakers is imperative, as they forge effective regulatory frameworks and champion innovative research to confront AMR head-on.

This Special Issue plunges into the depths of environmental AMR reservoirs, illuminating how these silent sentinels are sculpting the landscape of infectious disease management. Our mission is to amplify awareness and incite proactive measures to safeguard the potency of our most vital medicinal arsenal. We warmly invite submissions that intersect with environmental AMR. It features original research that delves into the genetic underpinnings of resistance, epidemiological studies charting the genetic spread, and critical reviews that amalgamate and distil current understanding while pinpointing areas for further exploration.

Dr. Mor Nadia Lurie-Weinberger
Prof. Dr. Dror Avisar
Guest Editors

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Keywords

  • antimicrobial resistance
  • AMR
  • environmental reservoirs
  • antibiotic resistance genes
  • ARGs
  • horizontal gene transfer

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Published Papers (1 paper)

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Research

19 pages, 4842 KiB  
Article
A Surveillance Study of Culturable and Antimicrobial-Resistant Bacteria in Two Urban WWTPs in Northern Spain
by Mario Sergio Pino-Hurtado, Rosa Fernández-Fernández, Allelen Campaña-Burguet, Carmen González-Azcona, Carmen Lozano, Myriam Zarazaga and Carmen Torres
Antibiotics 2024, 13(10), 955; https://doi.org/10.3390/antibiotics13100955 - 11 Oct 2024
Viewed by 1063
Abstract
Background/Objectives: Wastewater treatment plants (WWTPs) are hotspots for the spread of antimicrobial resistance into the environment. This study aimed to estimate the proportion of clinically relevant antimicrobial-resistant bacteria in two Spanish urban WWTPs, located in the region of La Rioja (Spain); Methods: Ninety-four [...] Read more.
Background/Objectives: Wastewater treatment plants (WWTPs) are hotspots for the spread of antimicrobial resistance into the environment. This study aimed to estimate the proportion of clinically relevant antimicrobial-resistant bacteria in two Spanish urban WWTPs, located in the region of La Rioja (Spain); Methods: Ninety-four samples (48 water/46 sludge) were collected and streaked on ten different selective media, in order to recover the culturable bacterial diversity with relevant resistance phenotypes: Extended-Spectrum β-Lactamase-producing Escherichia coli/Klebsiella pneumoniae (ESBL-Ec/Kp), Carbapenem-resistant Enterobacteriaceae (CR-E), Methicillin-resistant Staphylococcus aureus (MRSA), and Vancomycin-resistant Enterococcus faecium/faecalis (VR-E. faecium/faecalis). Isolates were identified by MALDI-TOF and were tested for antimicrobial susceptibility using the disk diffusion method. The confirmation of ESBL production was performed by the double-disk test; Results: A total of 914 isolates were recovered (31 genera and 90 species). Isolates with clinically relevant resistance phenotypes such as ESBL-Ec/Kp and CR-E were recovered in the effluent (0.4 × 100–4.8 × 101 CFU/mL) and organic amendment samples (1.0–101–6.0 × 102 CFU/mL), which are discharged to surface waters/agricultural fields. We reported the presence of VR-E. faecium in non-treated sludge and in the digested sludge samples (1.3 × 101–1 × 103 CFU/mL). MRSA was also recovered, but only in low abundance in the effluent (0.2 × 101 CFU/mL); Conclusions: This study highlights the need for improved wastewater technologies and stricter regulations on the use of amendment sludge in agriculture. In addition, regular monitoring and surveillance of WWTPs are critical for early detection and the mitigation of risks associated with the spread of antimicrobial resistance. Full article
(This article belongs to the Special Issue Tracking Reservoirs of Antimicrobial Resistance Genes in Environment)
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