Cell Sheet Engineering for Stem Cell Delivery and Tissue Modeling

A special issue of Bioengineering (ISSN 2306-5354).

Deadline for manuscript submissions: closed (31 March 2021) | Viewed by 8503

Special Issue Editor


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Guest Editor
1. Faculty of Medicine, Lomonosov Moscow State University, 119192 Moscow, Russia
2. Institute for Regenerative Medicine, Medical Research and Education Centre, Lomonosov Moscow State University, 119192 Moscow, Russia
Interests: regenerative medicine; wound healing; stem cell; tissue engineering; cell sheet; mesenchymal stem cell
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Special Issue Information

Cell sheet engineering has become a popular approach in the last couple of decades, mainly because of the obvious need for the better delivery of stem cells that undergo cell death after delivery by injection.

However, recent advances in this field have expanded our understanding of the cells’ biology within these multilayered constructs enriched by native extracellular matrix. At the moment, we believe that cell sheet engineering has gone far beyond an approach for the delivery of stem cells to treat disease, and has laid ground for next-level tissue modeling for both applied and basic research.

For this Special Issue, we invite cell biologists, specialists in ex vivo models, and tissue engineering as well as researchers in regenerative medicine to contribute their original research, reviews, hypotheses, and letters to provide an insight into the new achievements and directions in this field.

The Issue’s scope is focused on the cell sheets from different types of cells, their properties, and applications, and will give favor to new applications in tissue modeling and basic research regarding mechanisms of morphogenesis, regeneration, or cellular self-organization.

However, potential contributions are not limited to the above-mentioned, and communications on decellularized materials from cell sheets or the application of cell sheet-derived secretome or vesicles will be considered.

We expect that review submissions will be either invited or presented as an abstract for editorial consideration before submission of full-text manuscript. Experimental studies can be submitted according to the authors’ initiative within the deadlines, and will undergo editorial evaluation with peer review according to the journal’s rules of manuscript processing.

Dr. Pavel Makarevich
Guest Editor

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Keywords

  • Cell sheet
  • Adult stem cells
  • Tissue engineering
  • Cell therapy
  • Tissue models
  • 3D models and cultures

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Published Papers (2 papers)

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Research

16 pages, 7498 KiB  
Article
Measurement of the Adipose Stem Cells Cell Sheets Transmittance
by Jun Ochiai, Yutaka Niihara and Joan Oliva
Bioengineering 2021, 8(7), 93; https://doi.org/10.3390/bioengineering8070093 - 2 Jul 2021
Cited by 2 | Viewed by 2953
Abstract
In the field of cell therapy, the interest in cell sheet technology is increasing. To determine the cell sheet harvesting time requires experience and practice, and different factors could change the harvesting time (variability among donors and culture media, between cell culture dishes, [...] Read more.
In the field of cell therapy, the interest in cell sheet technology is increasing. To determine the cell sheet harvesting time requires experience and practice, and different factors could change the harvesting time (variability among donors and culture media, between cell culture dishes, initial cell seeding density). We have developed a device that can measure the transmittance of the multilayer cell sheets, using a light emitting diode and a light detector, to estimate the harvesting time. The transmittance of the adipose stromal cells cell sheets (ASCCS) was measured every other day as soon as the cells were confluent, up to 12 days. The ASCCS, from three different initial seeding densities, were harvested at 8, 10, and 12 days after seeding. Real-time PCR and immunostaining confirmed the expression of specific cell markers (CD29, CD73, CD90, CD105, HLA-A, HLA-DR), but less than the isolated adipose stromal cells. The number of cells per cell sheets, the average thickness per cell sheet, and the corresponding transmittance showed no correlation. Decrease of the transmittance seems to be correlated with the cell sheet maturation. For the first time, we are reporting the success development of a device to estimate ASCCS harvesting time based on their transmittance. Full article
(This article belongs to the Special Issue Cell Sheet Engineering for Stem Cell Delivery and Tissue Modeling)
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15 pages, 2994 KiB  
Article
Fibrin Glue Implants Seeded with Dental Pulp and Periodontal Ligament Stem Cells for the Repair of Periodontal Bone Defects: A Preclinical Study
by Natella I. Enukashvily, Julia A. Dombrovskaya, Anastasia V. Kotova, Natalia Semenova, Irina Karabak, Roman E. Banashkov, Dmitry Baram, Tatiana Paderina, Stanislav S. Bilyk, Wolf-Dieter Grimm, Anton N. Kovalenko, Dmitry Ivolgin, Egor M. Prikhodko and Alexey V. Silin
Bioengineering 2021, 8(6), 75; https://doi.org/10.3390/bioengineering8060075 - 1 Jun 2021
Cited by 9 | Viewed by 4541
Abstract
A technology to create a cell-seeded fibrin-based implant matching the size and shape of bone defect is required to create an anatomical implant. The aim of the study was to develop a technology of cell-seeded fibrin gel implant creation that has the same [...] Read more.
A technology to create a cell-seeded fibrin-based implant matching the size and shape of bone defect is required to create an anatomical implant. The aim of the study was to develop a technology of cell-seeded fibrin gel implant creation that has the same shape and size as the bone defect at the site of implantation. Using computed tomography (CT) images, molds representing bone defects were created by 3D printing. The form was filled with fibrin glue and human dental pulp stem cells (DPSC). The viability, set of surface markers and osteogenic differentiation of DPSC grown in fibrin gel along with the clot retraction time were evaluated. In mice, an alveolar bone defect was created. The defect was filled with fibrin gel seeded with mouse DPSC. After 28 days, the bone repair was analyzed with cone beam CT and by histological examination. The proliferation rate, set of surface antigens and osteogenic potential of cells grown inside the scaffold and in 2D conditions did not differ. In mice, both cell-free and mouse DPSC-seeded implants increased the bone tissue volume and vascularization. In mice with cell-seeded gel implants, the bone remodeling process was more prominent than in animals with a cell-free implant. The technology of 3D-printed forms for molding implants can be used to prepare implants using components that are not suitable for 3D printing. Full article
(This article belongs to the Special Issue Cell Sheet Engineering for Stem Cell Delivery and Tissue Modeling)
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