Advanced Enzyme-Based Sensors

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "(Bio)chemical Sensing".

Deadline for manuscript submissions: 25 February 2025 | Viewed by 1249

Special Issue Editors


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Guest Editor
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
Interests: immunoassays; MOF-based nanozymes; nanozyme-based sensors; DNA/RNA sensors

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Guest Editor Assistant
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China
Interests: raman/surface-enhanced raman spectroscopy; proteins; phospholipids and apoptosis

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Guest Editor Assistant
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
Interests: environmental analytical chemistry; rapid detection of pollutants; risk assessment of pollutants

Special Issue Information

Dear Colleagues,

The development of enzyme-based sensors has attracted much interest in the fields of biosensing, environmental monitoring, food analysis, disease diagnosis, and so on, showing great promise as important tools. These sensors are usually designed based on necessary principles for target recognition, signal amplification, signal transduction, and signal output.

In particular, enzyme-based reactions play a critical part in signal procurement, catalyzing a chemical reaction that produces a measurable change, such as a colorimetric, fluorescent, or electrochemical signal. They are widely used because many enzymes are well-characterized and readily available with good specificity, selectivity, and high efficiency. However, they always suffer from stability issues, as enzymes may denature over time or in certain conditions, requiring careful storage and handling. Thus, it is of great significance to optimize enzyme conformations and enhance enzyme activities, including promising enzyme hosts, interface or volume compartments, and so on. In addition, as an ideal alternative, nanozyme-based sensors also show great potential in analysis areas benefiting from the sufficient catalytic activity, stability, and high signal transduction capability of nanozymes.

In this Special Issue, we aim to attract outstanding researchers from diverse backgrounds focused on advanced enzyme-based sensors to share the latest research and insights. It also serves as a platform for discussing current challenges and advancements in the development of enzyme-based sensors as well as the characterization and application of traditional or emerging sensors. All kinds of research are welcomed in the Special Issue, including original articles, reviews, and perspectives that cover the following topics: enzyme-based sensor development, the integration of enzyme-based sensors with novel analytical technology, and the applications of enzyme-based sensors.

Dr. Nuanfei Zhu
Guest Editor

Dr. Jinyu Zhu
Dr. Dali Wei
Guest Editor Assistants

Manuscript Submission Information

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Keywords

  • enzyme-based sensor
  • enzyme cascade reaction
  • enzyme encapsulation
  • enzyme activity regulation
  • nanozyme-based sensor
  • biosensor
  • chemical sensor
  • wearable sensor

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Published Papers (1 paper)

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Review

28 pages, 2156 KiB  
Review
Sensing and Degradation of Organophosphorus Compounds by Exploitation of Heat-Loving Enzymes
by Giuseppe Manco, Eros A. Lampitella, Nagendra S. K. Achanta, Giuliana Catara, Maria Marone and Elena Porzio
Chemosensors 2025, 13(1), 12; https://doi.org/10.3390/chemosensors13010012 - 9 Jan 2025
Viewed by 897
Abstract
The increasing incidence of organophosphate (OP) pesticide poisoning and the use of OP chemical warfare agents (CWA) in conflicts and terrorist acts need sustainable methods for sensing, decontamination, and detoxification of OP compounds. Enzymes can serve as specific, cost-effective biosensors for OPs. We [...] Read more.
The increasing incidence of organophosphate (OP) pesticide poisoning and the use of OP chemical warfare agents (CWA) in conflicts and terrorist acts need sustainable methods for sensing, decontamination, and detoxification of OP compounds. Enzymes can serve as specific, cost-effective biosensors for OPs. We will report on recent advancements in the use of carboxylesterases from the Hormone-Sensitive Lipase for the detection of OP compounds. In addition, enzymatic-based OP detoxification and decontamination offer long-term, environmentally friendly benefits compared to conventional methods such as chemical treatment, incineration, neutralization, and volatilization. Enzymatic detoxification has gained attention as an alternative to traditional OP-detoxification methods. This review provides an overview of the latest research on enzymatic sensing and detoxification of OPs, by exploiting enzymes, isolated from thermophilic/extremophilic Bacteria and Archaea that show exceptional thermal stability and stability in other harsh conditions. Finally, we will make examples of integration between sensing and decontamination systems, including protein engineering to enhance OP-degrading activities and detailed characterization of the best variants. Full article
(This article belongs to the Special Issue Advanced Enzyme-Based Sensors)
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