Quantitative Sensing in the Microspace

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "Analytical Methods, Instrumentation and Miniaturization".

Deadline for manuscript submissions: closed (25 April 2022) | Viewed by 3969

Special Issue Editors


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Guest Editor
Department Solar Materials, Helmholtz Centre for Environmental Research (UFZ), Permoser Str. 15, D-04318 Leipzig, Germany
Interests: single-cell analysis; microfluidics; mass spectrometry; dielectrophoresis; chip-coupled analytics; fluorescence spectroscopy; biosensors; chemosensors

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Guest Editor
Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany
Interests: development and application of new microfluidic cultivation methods; investigation of population heterogeneity in bioprocesses; transfer, integration, and scale-up of single-cell data into laboratory scale; testing and optimizing miniaturized reactor concepts for new fields of application

Special Issue Information

Dear Colleagues,

Advances in microfluidics are revolutionizing life sciences and (bio)chemistry. Microfluidics enable environmental control in miniaturized reaction spaces and can be used for massively parallelized or accelerated analyses. Nonetheless, the application of microfluidics is often limited by analytical capabilities for detecting and quantifying analytes with the necessary sensitivity, specificity, and selectivity. Novel and innovative in situ approaches for the multimodal sensing of biological and chemical processes in the microspace are now emerging to keep pace with the rapid developments in microfluidics.

The applications of microspace sensing are numerous and encompass all classes of natural and synthetic molecules, such as cellular biomolecules and catalytic products. As their most important features, microspace probes and sensors should be integrable into microfluidics and respond in a specific and quantitative manner to target analyte concentrations and their dynamics.

The Special Issue aims to collect recent findings and advances in the quantitative sensing of analytes in microfluidic reaction environments. Researchers are invited to contribute research and review articles, as well as short communications, encompassing the broad range of disciplines from life sciences to chemistry.

Dr. Christian Dusny
Prof. Dr. Alexander Grünberger
Guest Editors

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Published Papers (1 paper)

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Research

12 pages, 1674 KiB  
Article
An Electrochemical and Raman Scattering Dual Detection Biosensor for Rapid Screening and Biomolecular Profiling of Cancer Biomarkers
by Shuvashis Dey, Emtiaz Ahmed, Pranjal Satishchandra Somvanshi, Abu Ali Ibn Sina, Alain Wuethrich and Matt Trau
Chemosensors 2022, 10(3), 93; https://doi.org/10.3390/chemosensors10030093 - 28 Feb 2022
Cited by 6 | Viewed by 3439
Abstract
Detecting circulating biomarkers sensitively and quantitatively is paramount for cancer screening, diagnosis, and treatment selection. Particularly, screening of a panel of circulating protein biomarkers followed by mapping of individual biomarkers could assist better diagnosis and understanding of the cancer progression mechanisms. Herein, we [...] Read more.
Detecting circulating biomarkers sensitively and quantitatively is paramount for cancer screening, diagnosis, and treatment selection. Particularly, screening of a panel of circulating protein biomarkers followed by mapping of individual biomarkers could assist better diagnosis and understanding of the cancer progression mechanisms. Herein, we present a miniaturized biosensing platform with dual readout schemes (electrochemical and Surface enhanced Raman scattering (SERS)) for rapid cancer screening and specific biomarker expressional profiling to support cancer management. Our approach utilizes a controlled nanomixing phenomena under alternative current electrohydrodynamic condition to improve the isolation of cancer-associated circulating proteins (i.e., Epidermal growth factor receptor (EGFR), BRAF, Programmed death-ligand 1 (PD-L1)) with antibody functionalized sensor surface for rapid and efficient isolation of the targets and subsequent labelling with SERS nanotags. The method employs Differential Pulse Voltammetry (DPV) for rapidly screening for the presence of the circulating proteins on biosensor surface irrespective of their type. Upon positive DPV detection, SERS is applied for sensitive read-out of individual biomarkers biomarker levels. In a proof-of-concept study, we demonstrate the dual detection biosensor for analysing circulating BRAF, EGFR and PDL-1 proteins and successfully screened both ensemble and individual biomarker expressional levels as low as 10 pg (1 ng/mL). Our findings clearly indicate the potential of the proposed method for cancer biomarker analysis which may drive the translation of this dual sensing concept in clinical settings. Full article
(This article belongs to the Special Issue Quantitative Sensing in the Microspace)
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