Advances in Germplasm Resources and Genetic Breeding of Aquatic Animals

A special issue of Fishes (ISSN 2410-3888). This special issue belongs to the section "Genetics and Biotechnology".

Deadline for manuscript submissions: 20 January 2025 | Viewed by 713

Special Issue Editors

College of Fisheries, Henan Normal University, Xinxiang, China
Interests: sex determination and differentiation; reproduction; development; oogenesis; gynogenesis
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Guest Editor
College of Fisheries, Guangdong Ocean University, Zhanjiang, China
Interests: reproduction; development; genetics; sex determination and differentiation; aquaculture
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
School of Marine Sciences, Ningbo University, Ningbo, China
Interests: fish; sex determination; sex differentiation; sex-control breeding

Special Issue Information

Dear Colleagues,

With the rapid development of aquaculture around the world, an increasing number of excellent new varieties are urgently needed. Meanwhile, a complete understanding of the range of germplasm resources and rudimentary breeding techniques will be more helpful in mitigating the current problems in fish breeding. This Special Issue will focus on how to retain the wild and farmed genetic resources that underpin global fish production, as well as the applied genetics approaches used for the genetic breeding of fish to increase aquaculture productivity and the conservation of fish genetic resources. Current technology, including genome selective breeding, sex-controlled breeding, and distant hybridization breeding, have shown significant efficiency. Genome selective breeding is one of the most advanced breeding techniques; on the other hand, many fish exhibit sexual dimorphism and new genes (or conventional genes with some potential undiscovered functions), and they are related to sex determination pathways that are yet to be explored. The aim of this Special Issue is to deepen our understanding of the theory and practice of fish genome selective breeding and the mechanisms of sex determination, as well as the analysis of hybridization traits.

Dr. Li-Min Wu
Dr. Dong-Neng Jiang
Dr. Qing-Ping Xie
Guest Editors

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Keywords

  • germplasm resources
  • genetic breeding
  • fish
  • sex control breeding
  • gonadal differentiation

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Published Papers (1 paper)

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Research

13 pages, 6946 KiB  
Article
Antibody Preparation, Protein Expression, and Function Analysis of Cyp19a1b in Ovarian Differentiation in a Natural Triploid Teleost Qi River Crucian Carp (Carassius auratus)
by Yongjing Li, Yujiao Jin, Limin Wu and Xuejun Li
Fishes 2024, 9(11), 442; https://doi.org/10.3390/fishes9110442 - 31 Oct 2024
Viewed by 458
Abstract
Estrogen is an essential sex steroid that functions in numerous biological systems including female reproduction, neuroendocrine, vascular, skeletal, and immune systems. The synthesis of estrogen is controlled by the rate-limiting enzyme, which has been confirmed to exist in two different forms, named brain [...] Read more.
Estrogen is an essential sex steroid that functions in numerous biological systems including female reproduction, neuroendocrine, vascular, skeletal, and immune systems. The synthesis of estrogen is controlled by the rate-limiting enzyme, which has been confirmed to exist in two different forms, named brain aromatase and ovary aromatase, and encoded by cyp19a1a and cyp19a1b respectively in teleosts. However, existing studies have primarily focused on the expression and function of cyp19a1b in the brain and cyp19a1a in the gonad, the roles of cyp19a1b in the female gonad of teleosts are largely unknown. In our previous study, we cloned the full length of the cyp19a1b gene from a natural triploid teleost Qi River crucian carp (Carassius auratus), andthe spatial and temporal expression patterns of cyp19a1b mRNA were detected. To further clarify the roles of cyp19a1b in the ovarian differentiation of Qi River crucian carp, we produced a polyclonal antibody of Cyp19a1b in this study. Western blotting results showed that Cyp19a1b was mainly expressed in the brain and then in the ovary, heart, liver, and muscle. During embryogenesis, Cyp19a1b was abundantly expressed in the neurula stage. Immunohistochemistry demonstrated that Cyp19a1b was expressed in the radioactive glial cells (RGCs) of the brain from 20 days after hatching (dah) and the somatic cells of the ovaries from 30 dah, the critical period of ovarian differentiation in Qi River crucian carp. With the treatment of letrozole, an inhibitor of the aromatase, the expression of Cyp19a1b was downregulated both in the brain and gonad. Our results suggested that Cyp19a1b might be involved in the development of the nervous system and also participate in the ovarian differentiation of Qi River crucian carp. Full article
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