Adaptive Mechanisms of Tree Seedlings to Adapt to Stress—Second Edition

A special issue of Forests (ISSN 1999-4907). This special issue belongs to the section "Forest Ecophysiology and Biology".

Deadline for manuscript submissions: closed (15 November 2024) | Viewed by 5214

Special Issue Editors

College of Life Sciences, Qufu Normal University, Qufu, China
Interests: plant regeneration; ecological strategy; functional traits; restoration ecology; phenotypic plasticity
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Guest Editor
College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China
Interests: forest ecology; climate change; silicon cycle; carnon cycle; plant–soil interaction
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Guest Editor
Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, Box 49, 23053 Alnarp, Sweden
Interests: regeneration; seedling physiology; light quality and quantity; seedling nutrition; seedling ecology
College of Life Sciences, Qufu Normal University, Qufu, China
Interests: janzen connell hypothesis; microbial functional flora; rhizosphere microorganisms; seeding regeneration; succession of microbial community
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

As the most critical stage throughout the plant life cycle, the seedling period plays a crucial role in forest community succession and vegetation restoration. Seedlings are vulnerable to biotic and abiotic stresses during their growth stage. Ongoing climate change is increasing the frequency and intensity of stresses such as drought, flood, extreme temperature, and pest spread. In the long-term evolutionary process, tree seedlings have developed a set of adaptive mechanisms to deal with these stresses. Research on seedling growth mechanisms is helpful to understand and clarify ecological adaptation characteristics that ensure better growth and performance in the field. For this Special Issue, we invite the submission of all research that deals with the adaptation mechanisms of tree seedlings to biotic and abiotic stresses, highlighting their important roles in coping with stresses in forest ecosystems. Thus, this Special Issue aims to collate up-to-date research findings on various adaptive mechanisms of tree seedlings to stress. You are welcome to browse the first edition of this Special Issue, “Adaptive Mechanisms of Tree Seedlings to Adapt to Stress”, at https://www.mdpi.com/journal/forests/special_issues/8O38081R1J.

Potential topics of interest for this Special Issue include, but are not limited to, the following:

  • Morphological, physiological, and ecological adaptation mechanisms;
  • Natural regeneration of tree species;
  • Adaptation mechanism of understory seedlings to different light conditions;
  • Intraspecific and interspecific competition mechanisms between seedlings;
  • Microbial community succession and their interaction with seedling growth;
  • Soil microorganisms and tree seedling growth;
  • Effects of extreme climate events on tree seedling survival and growth;
  • Silicon, microplastic, and site preparation methods and tree seedling growth;
  • Heavy metals and tree seedling growth.

Dr. Bo Liu
Dr. Shaofei Jin
Dr. Mulualem Tigabu
Dr. Jing Zhou
Guest Editors

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Keywords

  • adaptation
  • regeneration
  • seedlings
  • forest growth
  • climate change

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Published Papers (6 papers)

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Research

19 pages, 1831 KiB  
Article
Evaluation and Validation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis of the Gene Expression in Macadamia integrifolia
by Qian Yang, Ziping Yang, Hui Zeng, Minghong Zou, Ximei Song, Jifeng Wan, Zhao Wang, Jing Chen and Lianfang Luo
Forests 2024, 15(11), 1966; https://doi.org/10.3390/f15111966 - 7 Nov 2024
Viewed by 480
Abstract
Macadamia is an economically significant crop, with its kernel oil being abundant in monounsaturated fatty acids (MUFA). Analyzing the expression of genes related to MUFA biosynthesis is essential for understanding the complex regulatory networks in Macadamia. However, there are few reports on the [...] Read more.
Macadamia is an economically significant crop, with its kernel oil being abundant in monounsaturated fatty acids (MUFA). Analyzing the expression of genes related to MUFA biosynthesis is essential for understanding the complex regulatory networks in Macadamia. However, there are few reports on the identification of suitable reference genes for use as internal controls in this species. Consequently, selecting a reliable reference gene for gene expression studies under various conditions is critical. In this study, we evaluated the expression stability of 11 traditional housekeeping genes: α-tubulin (TUBa), β-tubulin (TUBb), malate dehydrogenase (MDH), 18S ribosomal RNA (18S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), α-elongation factor 1 (EF1a), β-elongation factor 1 (EF1b), ubiquitin (UBQ), ubiquitin-conjugating enzyme (UBC), cyclophilin (CYP), and actin (ACT) under abiotic stresses, hormonal treatments and in variety of plant tissues using the online tool RefFinder, which integrates four commonly used software programs: ΔCt, geNorm (version 3.4), NormFinder (version 0953), and BestKeeper (version 1.0). A comprehensive expression stability ranking was established by integrating results from these four methods based on the geometric mean. The findings indicated that ACT was the most stable gene across all samples, including those subjected to cold stress, NaCl stress, PEG stress, ABA treatment, MeJA treatment, and both stem and leaf tissues. EF1b was identified as the most stable gene in GA treatment and heat stress samples, while UBC and CYP were ranked highest in ethrel treatment and root tissue samples, respectively. Finally, the reliability of these findings was further validated using the target gene SAD through qRT-PCR. In summary, this study evaluated and validated appropriate reference genes for qRT-PCR, which will facilitate future investigations into the molecular mechanisms in Macadamia. Full article
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12 pages, 3186 KiB  
Article
Glycine Betaine Enhances Poplar Cultivar (Populus deltoides × Populus euramericana) Tolerance to Confront NaCl Stress
by Feiyan Chen, Ali Movahedi, Hui Wei, Qiang Zhuge and Weibo Sun
Forests 2024, 15(8), 1295; https://doi.org/10.3390/f15081295 - 25 Jul 2024
Viewed by 702
Abstract
Glycine betaine (GB) serves as a compatible solute crucial for stress tolerance across numerous plant species. Populus trichocarpa grows along coastal regions and often suffers from salt stress. In this study, we explored the effects of foliar spraying of GB on the growth, [...] Read more.
Glycine betaine (GB) serves as a compatible solute crucial for stress tolerance across numerous plant species. Populus trichocarpa grows along coastal regions and often suffers from salt stress. In this study, we explored the effects of foliar spraying of GB on the growth, physicochemical indexes, gene expression, and stress tolerance of poplar under salt stress. To achieve this, poplar plants (Populus deltoides × Populus euramericana “Nanlin 895”) were grown under four NaCl concentrations: 0, 50, 100, and 150 mM. Additionally, they were treated with 0, 5, 15, and 25 mM GB. The degree of effect on potted poplars was observed after 8 weeks. A GB treatment of about 15 mM significantly reduced the impact of salt stress and photosynthetic pigments and caused an improvement in the activity of the antioxidant enzymes peroxidase (POD) and superoxide dismutase (SOD). Moreover, GB-treated poplars had significantly more malonaldehyde (MDA) than the control lines. This study indicated that GB treatment reduces salt stress symptoms in poplars. This research helps to develop safe levels of endogenous GB, which will make poplars more resistant to environmental stresses. Full article
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13 pages, 3722 KiB  
Article
Temporal Variations in Enzymatic and Non-Enzymatic Antioxidant Activity in Silver Birch (Betula pendula Roth.): The Genetic Component
by Vaida Sirgedaitė-Šėžienė, Ieva Čėsnienė and Dorotėja Vaitiekūnaitė
Forests 2024, 15(7), 1262; https://doi.org/10.3390/f15071262 - 19 Jul 2024
Viewed by 958
Abstract
Betula pendula Roth. (silver birch) is a pioneer species in the Northern Hemisphere forests. It plays a significant role in various ecosystems, human industries, and biodiversity. Taking all this into account, understanding the genetic diversity within B. pendula populations is crucial for fully [...] Read more.
Betula pendula Roth. (silver birch) is a pioneer species in the Northern Hemisphere forests. It plays a significant role in various ecosystems, human industries, and biodiversity. Taking all this into account, understanding the genetic diversity within B. pendula populations is crucial for fully exploiting their potential, particularly regarding their production of phenolic compounds and antioxidants. We tested the non-enzymatic and enzymatic antioxidant activity in seven silver birch half-sib family leaves. Spectrophotometric data from leaf extracts showed that there was a significant variation between families in terms of total phenol content (TPC) and antioxidant enzyme (superoxide dismutase, peroxidase (POX), catalase, glutathione reductase, and ascorbate peroxidase) levels. The data were gathered during two consecutive seasons, resulting in a variance in antioxidant production, which generally increased in the tested families during the second year (except for POX) as opposed to the first vegetative season. For example, SOD levels increased in the second year by 15% to 243% and TPC increased by 46%–189%, depending on the half-sib family. A more thorough study of this variation should prove beneficial in various research fields, ranging from climate change to cosmetics. Full article
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16 pages, 1594 KiB  
Article
Natural Regeneration Patterns of Juglans mandshurica in Different Habitats
by Donghai Cui, Qingcheng Wang, Shuangjiao Ma, Yong Zhang, Liqing Xu and Limei Yan
Forests 2024, 15(7), 1201; https://doi.org/10.3390/f15071201 - 11 Jul 2024
Viewed by 693
Abstract
The natural regeneration of Juglans mandshurica (Juglans mandshurica Maxim.) is challenging. To elucidate its regeneration patterns, we conducted a comparative analysis of the spatial distribution and regeneration indices of Juglans mandshurica seedlings across different habitats, from the forest edge to the inner [...] Read more.
The natural regeneration of Juglans mandshurica (Juglans mandshurica Maxim.) is challenging. To elucidate its regeneration patterns, we conducted a comparative analysis of the spatial distribution and regeneration indices of Juglans mandshurica seedlings across different habitats, from the forest edge to the inner forest, over a span of 1000 m. Our findings revealed significant differences in seedling growth and regeneration patterns among these habitats. The growth indicators of Juglans mandshurica seedlings were notably higher at the forest edge compared to other habitats. The proportion of Juglans mandshurica seedlings was lower than that of 1- and 2-year-old seedlings, with only 7% being young seedlings at the forest edge. Juglans mandshurica renewal seedlings were aggregated, with the degree of aggregation as follows: 15 m > forest edge > 60 m > 30 m. The regeneration index was highest at the forest edge (1.0) and decreased with distance: 15 m (0.62), 30 m (0.52), 60 m (0.42). Shrub species and other seedlings at the forest edge showed a significant negative correlation with the Juglans mandshurica seedling numbers (p < 0.05). At 15 m, tree species displayed a significant (p < 0.05) or highly significant (p < 0.01) negative correlation. Stand closure at 30 m exhibited a substantial (p < 0.05) or highly significant (p < 0.01) negative correlation with the Juglans mandshurica regeneration index. At 60 m, the tree species, number, and basal area at breast height showed a significant (p < 0.05) or highly significant (p < 0.01) negative correlation with the Juglans mandshurica regeneration index. The forest edge had the highest renewal index and effectiveness, making it a priority for the conservation and management of Juglans mandshurica renewal. This study provides a theoretical foundation for the conservation and sustainable management of Juglans mandshurica populations. Full article
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25 pages, 4254 KiB  
Article
Effects of CO2 and Soil Moisture Treatments on Morphological and Allometric Trait Variation in Coppiced Seedlings: A Study of Four Early-Successional Deciduous Species
by Axel Brisebois and John E. Major
Forests 2024, 15(5), 856; https://doi.org/10.3390/f15050856 - 14 May 2024
Viewed by 876
Abstract
Atmospheric CO2 levels have been increasing, and likewise, increasing drought events have been following increasing temperatures. There is very little literature on the effects of climate change factors on early-successional deciduous species used for ecological restoration. Thus, morphological and allometric variation in [...] Read more.
Atmospheric CO2 levels have been increasing, and likewise, increasing drought events have been following increasing temperatures. There is very little literature on the effects of climate change factors on early-successional deciduous species used for ecological restoration. Thus, morphological and allometric variation in four coppiced early-successional deciduous species was examined in response to a 2 × 2 factorial of ambient CO2 (aCO2, 400 ppm) and elevated CO2 (eCO2, 800 ppm), as well as well-watered and drought treatments with 15%–20% and 5%–10% volumetric moisture content, respectively, grown in sandy soil with low soil nitrogen (N) under greenhouse conditions. The four species examined were as follows: green alder (Alnus viridis subsp. crispa (Ait.) Turrill), speckled alder (A. incana subsp. rugosa (Du Roi) R.T. Clausen), gray birch (Betula populifolia (Marshall)), and white birch (B. papyrifera (Marshall)), and all are from the same phylogenetic family, Betulaceae. Genus differences in morphological and growth traits were large, especially in response to the environmental treatments used. Alders upregulated all growth traits under eCO2 because of the strong coppicing sink effect and the additional foliar N provided by the actinorhizal ability of the genus, whereas birches remained the same or slightly decreased under eCO2. As a result, alders have a significantly greater foliar N than birches, with 2.8 and 1.0%, respectively. All species reduced growth under drought, and green alder had the greatest stem dry mass growth, followed by speckled alder and then the birches. Under drought, eCO2 not only mitigated the alder drought dry mass but, in fact, doubled the stem dm, whereas eCO2 only just mitigated the birches drought response. When corrected for size using stem height, alders allocated more to stem and leaf and less to root dry mass than birches. Atmospheric CO2 and soil moisture treatments changed organ biomass allocation. The tallest stem height was the best predictor of total (above and below) dry mass. With increasing atmospheric CO2, particularly on low nutrient sites, the results show alders are capable of sequestering far more carbon than birches. In addition, with more atmospheric CO2, alders can mitigate against drought conditions better compared to birches. Full article
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17 pages, 1470 KiB  
Article
The Influence of Minimal Cultivation Techniques on Growth Rate of Robinia pseudacacia L. Seedlings
by Cristina Drăghici, Ioan Vasile Abrudan, Adela Hoble, Raluca Enescu, Gheorghe Spârchez and Iacob Crăciunesc
Forests 2024, 15(5), 785; https://doi.org/10.3390/f15050785 - 29 Apr 2024
Viewed by 980
Abstract
The seed provenance, the type of substrate and its properties, as well as the watering regime in the first years after sowing are among the most important characteristics affecting the production of containerized seedlings. The objective of this study was to analyse the [...] Read more.
The seed provenance, the type of substrate and its properties, as well as the watering regime in the first years after sowing are among the most important characteristics affecting the production of containerized seedlings. The objective of this study was to analyse the growth of black locust (Robinia pseudacacia L.) seedlings on different types of substrate (six mineral and two organic) with different textures and pHs under three different regimes of available water content (no-limiting, medium drought, severe drought) over a period of five months (May–October 2023), using seeds from three sources located in the southeast, south, and west of Romania. The highest seedling emergence rate (73.7%) was obtained for the medium seeds with 18.492 g weight (1000 seed weighs) (Bucharest provenance). Direct sowing in the field and containerized seedlings both showed a trend of growing in height during unlimited water and of growing in diameter during low watering. The lowest survival rate of seedlings (86.7%) occurred in the mineral substrate in the case of rendzina with additional sand (pH 8.70), and for the organic substrate, the lowest survival rate (87.0%) occurred for the peat MKS 3 substrate (pH 4.54). Full article
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