Morphological and Highly Sensitive Detection (HSD) Observations of Monolayers

A special issue of Membranes (ISSN 2077-0375). This special issue belongs to the section "Biological Membranes".

Deadline for manuscript submissions: closed (10 November 2024) | Viewed by 2128

Special Issue Editors


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Guest Editor
Food & Energy Security Research & Product Center, Sendai, Japan
Interests: lipids; proteins; biosurfactants; biomolecular phase behavior; microbial extraction; application of microbial surfactants; microbes cultivation

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Guest Editor
Nagoya Institute of Technology, Nagoya, Japan
Interests: lipids; proteins; membrane channels; peptides; biomembranes; monolayers (natural lipids and synthetic lipids) Brewster angle microscopy; surface pressure-molecular area isotherms
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Special Issue Information

Dear Colleagues,

The morphology of various phospholipid and commercial monolayers is being investigated. The compression and dropping methods (CM and DM) are being used to prepare monolayers. In CM, monolayer constituent molecules are studded on the water surface then compressed with a partitioning board, whereas in DM, monolayer-forming molecules continue to be studded on the water surface until the monolayer is completed. Both natural lipids and commercial surfactants have been used to prepare monolayers on the water surface. Natural lipids mainly include dipalmitoyl phosphatidyl choline (DPPC) and dimyristoyl PC (DMPC).  Addition of the cholesterol to each monolayer has also been investigated. Apart from natural lipids, novel surfactants, dihexadecyl gemini phosphate (DHGP-n), with different alkyl spacers were used to obtain the surface properties of monolayers on the water surface.

A monolayer on the water surface is a simple system for investigating the surface properties and morphology such as phase transition from a liquid expanded (LE) to a liquid condensed (LC) state with the structural transition (gauche- to trans-conformation) of alkyl chains of lipid molecules. The research of the preparation and properties of monolayers on the water surface are important in explaining and applying various biological phenomena. The study has also applications in biomedical technologies.

We are pleased to invite you to contribute to the Special Issue “Morphological and Highly Sensitive Detection (HSD) Observations of Monolayers”. This Special Issue will contribute to the knowledge of understanding the interactions of small molecules, such as lipids or synthetic surfactant molecules, in a specific two-dimensional field. HSD will be useful in detecting the conformational changes occurring in the molecules under various conditions. Both research articles and reviews are welcome. The Special Issue will have a collection of at least 10 articles and may be printed in book form if this number is reached.

It covers (but not limited) the following topics: lipids and synthetic surfactants monolayers, BAM (Brewster angle microscopy), STm (surface tension measurement), surface pressure-molecular area isotherms, quartz crystal oscillator (QCO) method, the effects of small and large molecule additives on the monolayer properties. We look forward to receiving your contributions.

Dr. Zameer Shervani
Dr. Yasushi Yamamoto
Guest Editors

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Keywords

  • lipid monolayer
  • commercial surfactants monolayers
  • effect of additives
  • BAM (Brewster Angle Microscopy)
  • STm (Surface Tension measurement)
  • surface pressure-molecular area isotherms
  • quartz crystal oscillator (QCO) method
  • small and large molecules as additives

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Published Papers (1 paper)

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Research

19 pages, 2099 KiB  
Article
The Interaction between Anesthetic Isoflurane and Model-Biomembrane Monolayer Using Simultaneous Quartz Crystal Microbalance (QCM) and Quartz Crystal Impedance (QCI) Methods
by Yasushi Yamamoto, Daiki Ito, Honoka Akatsuka, Hiroki Noguchi, Arisa Matsushita, Hyuga Kinekawa, Hirotaka Nagano, Akihiro Yoshino, Keijiro Taga, Zameer Shervani and Masato Yamamoto
Membranes 2024, 14(3), 62; https://doi.org/10.3390/membranes14030062 - 27 Feb 2024
Viewed by 1722
Abstract
The interaction between anesthetic Isoflurane (Iso) and model-biomembrane on the water surface has been investigated using quartz crystal microbalance (QCM) and quartz crystal impedance (QCI) methods. The model-biomembranes used were dipalmitoyl phosphatidyl choline (DPPC), DPPC-palmitic acid (PA) mixture (DPPC:PA = 8:2), DPPC-Alamethicin (Al) [...] Read more.
The interaction between anesthetic Isoflurane (Iso) and model-biomembrane on the water surface has been investigated using quartz crystal microbalance (QCM) and quartz crystal impedance (QCI) methods. The model-biomembranes used were dipalmitoyl phosphatidyl choline (DPPC), DPPC-palmitic acid (PA) mixture (DPPC:PA = 8:2), DPPC-Alamethicin (Al) mixture (DPPC:Al = 39:1), and DPPC-β-Lactoglobulin (βLG) mixture (DPPC:βLG = 139:1) monolayers, respectively. The quartz crystal oscillator (QCO) was attached horizontally to each monolayer, and QCM and QCI measurements were performed simultaneously. It was found that Iso hydrate physisorbed on each monolayer/water interface from QCM and changed those interfacial viscosities from QCI. With an increase in Iso concentration, pure DPPC, DPPC-PA mixed, and DPPC-Al mixed monolayers showed a two-step process of Iso hydrate on both physisorption and viscosity, whereas it was a one-step for the DPPC-βLG mixed monolayer. The viscosity change in the DPPC-βLG mixed monolayer with the physisorption of Iso hydrate was much larger than that of other monolayers, in spite of the one-step process. From these results, the action mechanism of anesthetics and their relevance to the expression of anesthesia were discussed, based on the “release of interfacial hydrated water” hypothesis on the membrane/water interface. Full article
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