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Membranes
Special Issues
Biomembranes’ Involvement in Physiological Processes, and Tools for Their Characterization
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Biomembranes’ Involvement in Physiological Processes, and Tools for Their Characterization

A special issue of Membranes (ISSN 2077-0375). This special issue belongs to the section "Biological Membrane Functions".

Deadline for manuscript submissions: closed (30 April 2022) | Viewed by 15364

Special Issue Editor

Dr. Tomasz Trombik

E-Mail Website
Guest Editor
Faculty of Biotechnology, University of Wroclaw, 50-145 Wroclaw, Poland
Interests: molecular biology; biochemistry; biophysics; lipids; cell culture; nanobiotechnology; biotechnology; cell biology; cancer biology; drug resistance

Special Issue Information

Dear Colleagues,

Biomembranes are fascinating, dynamic structures, in which various lipids and proteins coexist and contribute to cell homeostasis. Many physiological processes rely on membranes—the intracellular membranes of different organelles and the cell plasma membrane. Ranging in function from simple diffusion through oxidative phosphorylation, vesicular transport to immunological synapse formation, membranes are integral elements of critical cellular processes essential for the whole organism's integrity and proper functioning, irrespectively of complexity and taxonomic classification. Despite some differences in structure and composition, the membranes of bacteria, fungi, plants and animals all play central and often similar roles in biological processes.  

This Special Issue aims to publish original articles, communications and reviews regarding the latest discoveries and research advancements in biological membranes and their roles in physiological processes and pathological states that may lead to disease. We would also appreciate studies in which innovative tools have been used to improve our understanding of membrane-driven processes.

Dr. Tomasz Trombik
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Membranes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Membrane lipids
  • Membrane lateral organization
  • Membrane dynamics
  • Membrane proteins
  • Membrane trafficking
  • Membrane transport
  • Membranes and disease

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Published Papers (4 papers)

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Research

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10 pages, 1832 KiB  
Article
Optimization of Membrane Protein TmrA Purification Procedure Guided by Analytical Ultracentrifugation
by Dongdong Li, Wendan Chu, Xinlei Sheng and Wenqi Li
Membranes 2021, 11(10), 780; https://doi.org/10.3390/membranes11100780 - 12 Oct 2021
Viewed by 2970
Abstract
Membrane proteins are involved in various cellular processes. However, purification of membrane proteins has long been a challenging task, as membrane protein stability in detergent is the bottleneck for purification and subsequent analyses. Therefore, the optimization of detergent conditions is critical for the [...] Read more.
Membrane proteins are involved in various cellular processes. However, purification of membrane proteins has long been a challenging task, as membrane protein stability in detergent is the bottleneck for purification and subsequent analyses. Therefore, the optimization of detergent conditions is critical for the preparation of membrane proteins. Here, we utilize analytical ultracentrifugation (AUC) to examine the effects of different detergents (OG, Triton X-100, DDM), detergent concentrations, and detergent supplementation on the behavior of membrane protein TmrA. Our results suggest that DDM is more suitable for the purification of TmrA compared with OG and TritonX-100; a high concentration of DDM yields a more homogeneous protein aggregation state; supplementing TmrA purified with a low DDM concentration with DDM maintains the protein homogeneity and aggregation state, and may serve as a practical and cost-effective strategy for membrane protein purification. Full article
(This article belongs to the Special Issue Biomembranes’ Involvement in Physiological Processes, and Tools for Their Characterization)
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12 pages, 1694 KiB  
Communication
High-Level Expression of Palmitoylated MPP1 Recombinant Protein in Mammalian Cells
by Agnieszka Chytła, Weronika Gajdzik-Nowak, Agnieszka Biernatowska, Aleksander F. Sikorski and Aleksander Czogalla
Membranes 2021, 11(9), 715; https://doi.org/10.3390/membranes11090715 - 17 Sep 2021
Cited by 3 | Viewed by 3044
Abstract
Our recent studies have pointed to an important role of the MAGUK family member, MPP1, as a crucial molecule interacting with flotillins and involved in the lateral organization of the erythroid plasma membrane. The palmitoylation of MPP1 seems to be an important element [...] Read more.
Our recent studies have pointed to an important role of the MAGUK family member, MPP1, as a crucial molecule interacting with flotillins and involved in the lateral organization of the erythroid plasma membrane. The palmitoylation of MPP1 seems to be an important element in this process; however, studies on the direct effect of palmitoylation on protein–protein or protein–membrane interactions in vitro are still challenging due to the difficulties in obtaining functional post-translationally modified recombinant proteins and the lack of comprehensive protocols for the purification of palmitoylated proteins. In this work, we present an optimized approach for the high-yield overexpression and purification of palmitoylated recombinant MPP1 protein in mammalian HEK-293F cells. The presented approach facilitates further studies on the molecular mechanism of lateral membrane organization and the functional impact of the palmitoylation of MPP1, which could also be carried out for other palmitoylated proteins. Full article
(This article belongs to the Special Issue Biomembranes’ Involvement in Physiological Processes, and Tools for Their Characterization)
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15 pages, 1314 KiB  
Article
Cumulative Fluid Balance during Extracorporeal Membrane Oxygenation and Mortality in Patients with Acute Respiratory Distress Syndrome
by Li-Chung Chiu, Li-Pang Chuang, Shih-Wei Lin, Yu-Ching Chiou, Hsin-Hsien Li, Yung-Chang Chen, Yu-Jr Lin, Chee-Jen Chang, Feng-Chun Tsai, Ko-Wei Chang, Han-Chung Hu, Chung-Chi Huang, Shaw-Woei Leu and Kuo-Chin Kao
Membranes 2021, 11(8), 567; https://doi.org/10.3390/membranes11080567 - 28 Jul 2021
Cited by 6 | Viewed by 2865
Abstract
Extracorporeal membrane oxygenation (ECMO) is considered a salvage therapy in cases of severe acute respiratory distress syndrome (ARDS) with profound hypoxemia. However, the need for high-volume fluid resuscitation and blood transfusions after ECMO initiation introduces a risk of fluid overload. Positive fluid balance [...] Read more.
Extracorporeal membrane oxygenation (ECMO) is considered a salvage therapy in cases of severe acute respiratory distress syndrome (ARDS) with profound hypoxemia. However, the need for high-volume fluid resuscitation and blood transfusions after ECMO initiation introduces a risk of fluid overload. Positive fluid balance is associated with mortality in critically ill patients, and conservative fluid management for ARDS patients has been shown to shorten both the duration of mechanical ventilation and time spent in intensive care, albeit without a significant effect on survival. Nonetheless, few studies have addressed the influence of fluid balance on clinical outcomes in severe ARDS patients undergoing ECMO. In the current retrospective study, we examined the impact of cumulative fluid balance (CFB) on hospital mortality in 152 cases of severe ARDS treated using ECMO. Overall hospital mortality was 53.3%, and we observed a stepwise positive correlation between CFB and the risk of death. Cox regression models revealed that CFB during the first 3 days of ECMO was independently associated with higher hospital mortality (adjusted hazard ratio 1.110 [95% CI 1.027–1.201]; p = 0.009). Our findings indicate the benefits of a conservative treatment approach to avoid fluid overload during the early phase of ECMO when dealing with severe ARDS patients. Full article
(This article belongs to the Special Issue Biomembranes’ Involvement in Physiological Processes, and Tools for Their Characterization)
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Review

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43 pages, 3663 KiB  
Review
Polyene Antibiotics Physical Chemistry and Their Effect on Lipid Membranes; Impacting Biological Processes and Medical Applications
by Tammy Haro-Reyes, Lucero Díaz-Peralta, Arturo Galván-Hernández, Anahi Rodríguez-López, Lourdes Rodríguez-Fragoso and Iván Ortega-Blake
Membranes 2022, 12(7), 681; https://doi.org/10.3390/membranes12070681 - 30 Jun 2022
Cited by 21 | Viewed by 5517
Abstract
This review examined a collection of studies regarding the molecular properties of some polyene antibiotic molecules as well as their properties in solution and in particular environmental conditions. We also looked into the proposed mechanism of action of polyenes, where membrane properties play [...] Read more.
This review examined a collection of studies regarding the molecular properties of some polyene antibiotic molecules as well as their properties in solution and in particular environmental conditions. We also looked into the proposed mechanism of action of polyenes, where membrane properties play a crucial role. Given the interest in polyene antibiotics as therapeutic agents, we looked into alternative ways of reducing their collateral toxicity, including semi-synthesis of derivatives and new formulations. We follow with studies on the role of membrane structure and, finally, recent developments regarding the most important clinical applications of these compounds. Full article
(This article belongs to the Special Issue Biomembranes’ Involvement in Physiological Processes, and Tools for Their Characterization)
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