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Micromachines
Special Issues
Micro/Nanofluidics for Cell and Particle Manipulation
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Micro/Nanofluidics for Cell and Particle Manipulation

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B：Biology and Biomedicine".

Deadline for manuscript submissions: closed (31 August 2022) | Viewed by 8413

Special Issue Editors

Dr. Jeong Hun Nam

E-Mail Website
Guest Editor
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea
Interests: microfluidics; surface acoustic wave; viscoelasticity; biosensing; biotechnology
Dr. Hyunjung Lim

E-Mail Website
Guest Editor
Department of Medical Sciences, College of Medicine, Korea University, Seoul 08307, Korea
Interests: hemorheology; microfluidics; biomedical applications

Special Issue Information

Dear Colleagues,

Manipulation techniques of micro/nanoparticles and cells have gained much attention in sample preparation and analysis for biological and clinical applications. Particularly, microfluidic techniques are widely used due to the advantages of fast processing time, small volume of samples and reagents, miniaturized size, and high accuracy at a reduced cost. Microfluidic particle/cell manipulation techniques are classified into two types: active and passive methods depending on the use of external force fields. In this Special Issue, a wide range of topics are covered, including the design and fabrication of novel microfluidic devices for particle/cell manipulation, numerical, and/or experimental analysis of microfluidic manipulation techniques, and applications of micro/nanofluidic techniques for biological and clinical applications.

Dr. Jeong Hun Nam
Dr. Hyunjung Lim
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Biotechnology
  • Micro/nanofluidics
  • Micro/nanoparticle
  • Manipulation
  • Sensing

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Published Papers (3 papers)

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Research

15 pages, 13670 KiB  
Article
Development of a Simple Fabrication Method for Magnetic Micro Stir Bars and Induction of Rotational Motion in Chlamydomonas reinhardtii
by Ichiro Shimizu, Kyohei Yamashita and Eiji Tokunaga
Micromachines 2022, 13(11), 1842; https://doi.org/10.3390/mi13111842 - 27 Oct 2022
Cited by 1 | Viewed by 2004
Abstract
A magnetic micro stirrer bar (MMSB) is used in the mixing operation of microfluidic devices. We have established a low-cost and easy method to make MMSBs using magnetic (neodymium magnets, magnet sheets) or non-magnetic powders (SUS304) as materials. We demonstrated three kinds of [...] Read more.
A magnetic micro stirrer bar (MMSB) is used in the mixing operation of microfluidic devices. We have established a low-cost and easy method to make MMSBs using magnetic (neodymium magnets, magnet sheets) or non-magnetic powders (SUS304) as materials. We demonstrated three kinds of MMSB have respective advantages. To confirm the practical use of this MMSB, a cell suspension of the motile unicellular green alga Chlamydomonas reinhardtii was stirred in microwells. As a result, the number of rotating cells increased with only one of the two flagella mechanically removed by the shear force of the rotating bar, which facilitates the kinetic analysis of the flagellar motion of the cell. The rotational motion of the monoflagellate cell was modeled as translational (orbital) + spinning motion of a sphere in a viscous fluid and the driving force per flagellum was confirmed to be consistent with previous literature. Since the present method does not use genetic manipulations or chemicals to remove a flagellum, it is possible to obtain cells in a more naturally viable state quickly and easily than before. However, since the components eluted from the powder material harm the health of cells, it was suggested that MMSB coated with resin for long-term use would be suitable for more diverse applications. Full article
(This article belongs to the Special Issue Micro/Nanofluidics for Cell and Particle Manipulation)
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11 pages, 2038 KiB  
Article
A Continuous Microfluidic Concentrator for High-Sensitivity Detection of Bacteria in Water Sources
by Seunghee Choo, Hyunjung Lim, Tae Eun Kim, Jion Park, Kyu Been Park, Chaewon Park, Chae Seung Lim and Jeonghun Nam
Micromachines 2022, 13(7), 1093; https://doi.org/10.3390/mi13071093 - 10 Jul 2022
Cited by 3 | Viewed by 1943
Abstract
Water contamination is a critical issue that threatens global public health. To enable the rapid and precise monitoring of pathogen contamination in drinking water, a concentration technique for bacterial cells is required to address the limitations of current detection methods, including the culture [...] Read more.
Water contamination is a critical issue that threatens global public health. To enable the rapid and precise monitoring of pathogen contamination in drinking water, a concentration technique for bacterial cells is required to address the limitations of current detection methods, including the culture method and polymerase chain reaction. Here we present a viscoelastic microfluidic device for the continuous concentration of bacterial cells. To validate the device performance for cell concentration, the flow characteristics of 2-μm particles were estimated in viscoelastic fluids at different concentrations and flow rates. Based on the particle flow distributions, the flow rate factor, which is defined as the ratio of the inlet flow rate to the outlet flow rate at the center outlet, was optimized to achieve highly concentrated bacterial cells by removal of the additional suspending medium. The flow characteristics of 0.5-, 0.7-, and 1.0-μm-diameter particles were evaluated to consider the effect of a wide spectrum of bacterial size distribution. Finally, the concentration factor of bacterial cells, Staphylococcus aureus, suspended in a 2000-ppm polyethylene oxide solution was found to be 20.6-fold at a flow rate of 20 μL/min and a flow rate factor of 40. Full article
(This article belongs to the Special Issue Micro/Nanofluidics for Cell and Particle Manipulation)
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14 pages, 3053 KiB  
Article
High-Throughput Cell Concentration Using A Piezoelectric Pump in Closed-Loop Viscoelastic Microfluidics
by Jeeyong Kim, Hyunjung Lim, Hyunseul Jee, Seunghee Choo, Minji Yang, Sungha Park, Kyounghwa Lee, Hyoungsook Park, Chaeseung Lim and Jeonghun Nam
Micromachines 2021, 12(6), 677; https://doi.org/10.3390/mi12060677 - 9 Jun 2021
Cited by 4 | Viewed by 3057
Abstract
Cell concentration is a critical process in biological assays and clinical diagnostics for the pre-treatment of extremely rare disease-related cells. The conventional technique for sample preconcentration and centrifugation has the limitations of a batch process requiring expensive and large equipment. Therefore, a high-throughput [...] Read more.
Cell concentration is a critical process in biological assays and clinical diagnostics for the pre-treatment of extremely rare disease-related cells. The conventional technique for sample preconcentration and centrifugation has the limitations of a batch process requiring expensive and large equipment. Therefore, a high-throughput continuous cell concentration technique needs to be developed. However, in single-pass operation, the required concentration ratio is hard to achieve. In this study, we propose a closed-loop continuous cell concentration system using a viscoelastic non-Newtonian fluid. For miniaturized and integrated systems, two piezoelectric pumps were adopted. The pumping capability generated by a piezoelectric pump in a microfluidic channel was evaluated depending on the applied voltage, frequency, sample viscosity, and channel length. The concentration performance of the device was evaluated using 13 μm particles and white blood cells (WBCs) with different channel lengths and voltages. In the closed-loop system, the focused cells collected at the center outlet were sent back to the inlet, while the buffer solution was removed to the side outlets. Finally, to expand the clinical applicability of our closed-loop system, WBCs in lysed blood samples with 70% hematocrit and prostate cancer cells in urine samples were used. Using the closed-loop system, WBCs were concentrated by ~63.4 ± 0.8-fold within 20 min to a final volume of 160 μL using 10 mL of lysed blood sample with 70% hematocrit (~3 cP). In addition, prostate cancer cells in 10 mL urine samples were concentrated by ~64.1-fold within ~11 min due to low viscosity (~1 cP). Full article
(This article belongs to the Special Issue Micro/Nanofluidics for Cell and Particle Manipulation)
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