Microfluidics for Cell Detection and Sorting

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B:Biology and Biomedicine".

Deadline for manuscript submissions: closed (1 March 2021) | Viewed by 9714

Special Issue Editor


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Guest Editor
Department of Bioengineeering, Imperial College London, London SW7 2AZ, UK
Interests: microfluidics; droplet microfluidics; detection; sorting; surface acoustic waves; imaging; raman spectroscopy
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Special Issue Information

Recent advances in microfluidic cell detection and sorting techniques have enabled biologists to gain invaluable insight into biological functions associated with cell heterogeneity. Applications of these techniques include cancer research, directed evolution studies, environmental sampling, personalised medicine, drinking water monitoring and more. Even though there are powerful cell detection and sorting techniques, such as fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS), they have their limitations. Therefore, there is a strong need for specialised cell detection and sorting techniques.

Lately, it has become significantly harder to communicate pure engineering studies in this field. Such studies may include reporting a new on-chip detection technique or describing a novel sorting technique with advantages over its counterparts. This Special Issue aims to collate such studies to disseminate this research and attract wider attention. You are cordially invited to submit research papers, short communications, and review articles to this Special Issue in Micromachines titled “Microfluidics for Cell Detection and Sorting”.

Dr. Muhsincan Sesen
Guest Editor

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Keywords

  • Microfluidics
  • Cell Detection
  • Cell Sorting
  • Lab-on-a-Chip
  • Droplet Microfluidics
  • Label-Free

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Published Papers (2 papers)

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Research

12 pages, 4650 KiB  
Communication
Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting
by Alex A. Zhukov, Robyn H. Pritchard, Mick J. Withers, Tony Hailes, Richard D. Gold, Calum Hayes, Mette F. la Cour, Fred Hussein and Salman Samson Rogers
Micromachines 2021, 12(4), 389; https://doi.org/10.3390/mi12040389 - 2 Apr 2021
Cited by 10 | Viewed by 4649
Abstract
We demonstrate extremely high-throughput microfluidic cell sorting by making a parallel version of the vortex-actuated cell sorter (VACS). The set-up includes a parallel microfluidic sorter chip and parallel cytometry instrumentation: optics, electronics and control software. The result is capable of sorting lymphocyte-sized particles [...] Read more.
We demonstrate extremely high-throughput microfluidic cell sorting by making a parallel version of the vortex-actuated cell sorter (VACS). The set-up includes a parallel microfluidic sorter chip and parallel cytometry instrumentation: optics, electronics and control software. The result is capable of sorting lymphocyte-sized particles at 16 times the rate of our single-stream VACS devices, and approximately 10 times the rate of commercial cell sorters for an equivalent procedure. We believe this opens the potential to scale cell sorting for applications requiring the processing of much greater cell numbers than currently possible with conventional cell sorting. Full article
(This article belongs to the Special Issue Microfluidics for Cell Detection and Sorting)
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16 pages, 7134 KiB  
Article
Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel
by Ann-Kathrin Reichenwallner, Esma Vurmaz, Kristina Battis, Laura Handl, Helin Üstün, Tivadar Mach, Gabriele Hörnig, Jan Lipfert and Lukas Richter
Micromachines 2021, 12(4), 358; https://doi.org/10.3390/mi12040358 - 26 Mar 2021
Cited by 5 | Viewed by 4415
Abstract
We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) [...] Read more.
We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) is feasible. Cell count variations in between measurements differed by 2.47% with a deviation of 0.26×106 μL to the reference value obtained from the Siemens ADVIA 2120i. Measured MCV values varied by 2.25% and CHCM values by 3.78% compared to the reference ADVIA measurement. Our results suggest that the combination of optical analysis with microfluidics handling provides a promising new approach to red blood cell counts. Full article
(This article belongs to the Special Issue Microfluidics for Cell Detection and Sorting)
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