Microfluidics for Cells and Other Organisms, Volume III

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B:Biology and Biomedicine".

Deadline for manuscript submissions: closed (31 January 2022) | Viewed by 7912

Special Issue Editor


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Guest Editor
National Heart Centre Singapore, Singapore, Singapore
Interests: microfluidics; 3D cell cultures; human-on-a-chip; lab automation; biomedical engineering
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Special Issue Information

Dear Colleagues,

I would like to invite you to submit your research on cells and other organisms in microfluidics. Microfluidics-based devices play an important role in creating realistic microenvironments in which cell cultures can thrive. They can, for example, be used to monitor drug toxicity and perform medical diagnostics, and be in a static-, perfusion- or droplet-based device. They can also be used to study cell-cell, cell-matrix or cell-surface interactions. Cells can be either single cells, 3D cell cultures or co-cultures. Other organisms could include bacteria, zebra fish embryo, C. elegans, to name a few. In addition, research contributions on plant cells and plants in microfluidics are encouraged. However, we will not be considering cancer models, as that will be the subject for a separate Special Issue in Bioengineering later on.

This Special Issue will give you the opportunity to publish work that has not fully matured yet, but is worthwhile to be brought to the attention of other researchers and readers of the journal.

Dr. Danny van Noort
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Microfluidics
  • Bacteria
  • Cells
  • Cell Cultures
  • Tissue
  • Organisms

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Published Papers (2 papers)

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Research

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14 pages, 23161 KiB  
Article
An Automatic Platform Based on Nanostructured Microfluidic Chip for Isolating and Identification of Circulating Tumor Cells
by Hei-Jen Jou, Li-Yun Chou, Wen-Chun Chang, Hsin-Cheng Ho, Wan-Ting Zhang, Pei-Ying Ling, Ko-Hsin Tsai, Szu-Hua Chen, Tze-Ho Chen, Pei-Hsuan Lo, Ming Chen and Heng-Tung Hsu
Micromachines 2021, 12(5), 473; https://doi.org/10.3390/mi12050473 - 21 Apr 2021
Cited by 20 | Viewed by 4190
Abstract
Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with the CTC detection have limited its potential impact on clinical application. A reliable automated CTC detection system is therefore needed. We [...] Read more.
Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with the CTC detection have limited its potential impact on clinical application. A reliable automated CTC detection system is therefore needed. We have designed an automated microfluidic chip-based CTC detection system and hypothesize this novel system can reliably detect CTC from clinical specimens. SKOV3 ovarian cancer cell line was used first to test the reliability of our system. Ten healthy volunteers, 5 patients with benign ovarian tumors, and 8 patients with epithelial ovarian cancer (EOC) were recruited to validate the CTC capturing efficacy in the peripheral blood. The capture rates for spiking test in SKOV3 cells were 48.3% and 89.6% by using anti-EpCAM antibody alone and a combination of anti-EpCAM antibody and anti-N-cadherin antibody, respectively. The system was sensitive to detection of low cell count and showed a linear relationship with the cell counts in our test range. The sensitivity and specificity were 62.5% and 100% when CTC was used as a biomarker for EOC. Our results demonstrated that this automatic CTC platform has a high capture rate and is feasible for detection of CTCs in EOC. Full article
(This article belongs to the Special Issue Microfluidics for Cells and Other Organisms, Volume III)
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Review

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14 pages, 2366 KiB  
Review
Systematic Review: Microfluidics and Plasmodium
by Nicolas Thorne, Luis Flores-Olazo, Rocío Egoávil-Espejo, Emir A. Vela, Julien Noel, Julio Valdivia-Silva and Danny van Noort
Micromachines 2021, 12(10), 1245; https://doi.org/10.3390/mi12101245 - 14 Oct 2021
Cited by 5 | Viewed by 3246
Abstract
Malaria affects 228 million people worldwide each year, causing severe disease and worsening the conditions of already vulnerable populations. In this review, we explore how malaria has been detected in the past and how it can be detected in the future. Our primary [...] Read more.
Malaria affects 228 million people worldwide each year, causing severe disease and worsening the conditions of already vulnerable populations. In this review, we explore how malaria has been detected in the past and how it can be detected in the future. Our primary focus is on finding new directions for low-cost diagnostic methods that unspecialized personnel can apply in situ. Through this review, we show that microfluidic devices can help pre-concentrate samples of blood infected with malaria to facilitate the diagnosis. Importantly, these devices can be made cheaply and be readily deployed in remote locations. Full article
(This article belongs to the Special Issue Microfluidics for Cells and Other Organisms, Volume III)
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