Structure and Composition of Biofilms of Cutaneous and Environmental Microorganisms

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Biofilm".

Deadline for manuscript submissions: closed (31 October 2021) | Viewed by 4025

Special Issue Editor


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Guest Editor
Research Unit Bacterial Communication and Anti-infectious Strategies (UR CBSA), University of Rouen Normandie, 27000 Evreux, France
Interests: bacterial communication; bacterial virulence; skin-bacteria-cosmetics interactions; endocrine microbiology; cutaneous microbiota
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Special Issue Information

Dear Colleagues,

Our vision of biofilms evolved in parallel with our knowledge of their complexity. Bacterial biofilms have been the topic of more studies, but yeasts, fungi, and archaea also develop as biofilms, and cutaneous or environmental biofilms include a large diversity of microorganisms. In biofilms, microorganisms undergo local adaptations, as observed for bacterial persisters. The 3D structure of biofilms and their organization remain an important question, particularly in mixed populations. The exact composition of the biofilm matrix is poorly known, as it is particularly difficult to separate it from the outer surface of the microorganisms and the microenvironment. As was recently shown, the dynamics of the biofilms depends on communication between microorganisms, but also on the perception of environmental and host factors. Compounds involved in this process include classical soluble molecules as well as volatile substances and even vesicles. Even the existence of nanowire-dependent electrical communication remains a topic of discussion in environmental biofilms. We can also interrogate the role of phages and other viruses in the context of biofilms. As such, studying biofilms remains a topical challenge, requiring the mobilization of transdisciplinary knowledge and techniques.

The aim of this Special Issue is to provide an up-to-date multidisciplinary platform for the interchange of information on these fascinating organizations that we designate as biofilms, particularly in the context of skin and environment.

As Guest Editor of this Special Issue, I invite you to submit research articles, review articles, and short communications related to the structure, composition, and dynamics of cutaneous and environmental biofilms.

Prof. Dr. Marc G.J. Feuilloley
Guest Editor

Keywords

  • complex biofilms
  • biofilm structure
  • biofilm matrix
  • biofilm dynamics
  • microbial communication

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Research

12 pages, 463 KiB  
Article
Relationship between the Biofilm-Forming Capacity and Antimicrobial Resistance in Clinical Acinetobacter baumannii Isolates: Results from a Laboratory-Based In Vitro Study
by Matthew Gavino Donadu, Vittorio Mazzarello, Piero Cappuccinelli, Stefania Zanetti, Melinda Madléna, Ádám László Nagy, Anette Stájer, Katalin Burián and Márió Gajdács
Microorganisms 2021, 9(11), 2384; https://doi.org/10.3390/microorganisms9112384 - 18 Nov 2021
Cited by 30 | Viewed by 3350
Abstract
The relationship between the multidrug-resistant (MDR) phenotype and biofilm-forming capacity has been a topic of extensive interest among biomedical scientists, as these two factors may have significant influence on the outcomes of infections. The aim of the present study was to establish a [...] Read more.
The relationship between the multidrug-resistant (MDR) phenotype and biofilm-forming capacity has been a topic of extensive interest among biomedical scientists, as these two factors may have significant influence on the outcomes of infections. The aim of the present study was to establish a possible relationship between biofilm-forming capacity and the antibiotic-resistant phenotype in clinical Acinetobacter baumannii (A. baumannii) isolates. A total of n = 309 isolates were included in this study. Antimicrobial susceptibility testing and the phenotypic detection of resistance determinants were carried out. The capacity of isolates to produce biofilms was assessed using a crystal violet microtiter-plate-based method. Resistance rates were highest for ciprofloxacin (71.19%; n = 220), levofloxacin (n = 68.61%; n = 212), and trimethoprim-sulfamethoxazole (n = 66.02%; n = 209); 42.72% (n = 132) of isolates were classified as MDR; 22.65% (n = 70) of tested isolates were positive in the modified Hodge-test; the overexpression of efflux pumps had significant effects on the susceptibilities of meropenem, gentamicin, and ciprofloxacin in 14.24% (n = 44), 6.05% (n = 19), and 27.51% (n = 85), respectively; 9.39% (n = 29), 12.29% (n = 38), 22.97% (n = 71), and 55.35% (n = 170) of isolates were non-biofilm-producing and weak, moderate, and strong biofilm producers, respectively. A numerical, but statistically not significant, difference was identified between the MDR and non-MDR isolates regarding their biofilm-forming capacity (MDR: 0.495 ± 0.309 vs. non-MDR: 0.545 ± 0.283; p = 0.072), and no association was seen between resistance to individual antibiotics and biofilm formation. Based on numerical trends, MER-resistant isolates were the strongest biofilm producers (p = 0.067). Our study emphasizes the need for additional experiments to assess the role biofilms have in the pathogenesis of A. baumannii infections. Full article
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