Microbial Biotechnological Application for Metabolite Bioprocesses

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: 31 March 2025 | Viewed by 1586

Special Issue Editor


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Guest Editor
Department of Agricultural Chemistry, College of Bio-Resource and Agriculture, National Taiwan University, Taipei 10617, Taiwan
Interests: molecular biology; biotechnology; microbial physiology; enzymatic biochemistry and biodegradation

Special Issue Information

Dear Colleagues,

A Special Issue on “Microbial Biotechnological Application for Metabolite Bioprocesses” is being prepared for the journal Microorganisms. A bioprocess with microbial biotechnology is an application of the microbial bio-process technique involving living microorganisms or their enzymatic components to influence the desired bio-transformation of substrates. In contrast to traditional chemical processes, their mild and/or non-toxic reaction conditions are used for the specific and efficient production of valuable compounds with multiple applications. In addition, advanced recombinant DNA technology has enhanced the potential of microbial biotechnology in bioprocesses. Many fields have successfully adopted microbial biotechnology in bioprocesses including industrial production of biochemicals, production of secondary metabolites, production of flavors, food and beverages, production of pharmaceutical biosynthesis, and other metabolites. Recently, metabolic engineering, synthetic biology, and systems biology have enabled microorganisms to produce diverse and novel biochemicals. Thus, developing novel effective tools and understanding microbial metabolisms are essential for the microbial biotechnological applications in bioprocesses, which will accelerate the development of economic productions of many value-added bioactive compounds. Potential topics of interest in this Special Issue include but are not limited to the following:

  • Production of natural and bioactive compounds in bioprocesses by microorganisms;
  • Production of non-native chemicals in bioprocesses by microorganisms;
  • Microorganism metabolic engineering involved in bioprocesses;
  • Application of systems biology and synthetic biology in microorganism biotechnology in bioprocesses;
  • Genome-scale models of microorganisms; novel microorganism genome editing tool development;
  • Microorganism omics data application in microorganism biotechnology in bioprocesses.

Dr. Whei-Fen Wu
Guest Editor

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Keywords

  • microbial biotechnology
  • bioprocess
  • metabolic engineering
  • genome editing tool
  • omics
  • recombinant DNA technology

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Published Papers (1 paper)

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Research

18 pages, 1492 KiB  
Article
Recovery of Phenolic Compounds with Antioxidant Capacity Through Solid-State Fermentation of Pistachio Green Hull
by Andrés Javier Ordoñez-Cano, Ulises Ramírez-Esparza, Fernando Méndez-González, Mónica Alvarado-González, Ramiro Baeza-Jiménez, Leonardo Sepúlveda-Torre, Lilia Arely Prado-Barragán and José Juan Buenrostro-Figueroa
Microorganisms 2025, 13(1), 35; https://doi.org/10.3390/microorganisms13010035 - 27 Dec 2024
Viewed by 1131
Abstract
Pistachio green hull (PGH) represents the non-edible fraction obtained after the seed is harvested and is an important source of phenolic compounds. Solid-state fermentation (SSF) is a viable biotechnological and economical technique for extracting phenolic compounds. This study aimed to evaluate the SSF [...] Read more.
Pistachio green hull (PGH) represents the non-edible fraction obtained after the seed is harvested and is an important source of phenolic compounds. Solid-state fermentation (SSF) is a viable biotechnological and economical technique for extracting phenolic compounds. This study aimed to evaluate the SSF with Aspergillus niger GH1 to recover total phenolic compounds (TPC) with antioxidant capacity (AC) from PGH. For this, the time of higher TPC and AC (DPPH [2,2-diphenyl-1-picrylhydrazyl], ABTS [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate)], FRAP [ferric reducing antioxidant power]) was selected. Then, moisture, inoculum concentration, and aeration rate were evaluated. A. niger GH1 was able to grow and colonize the PGH, with the higher value of TPC (23.83 mg/g of dry mass (gdm)) obtained after 24 h of culture, which significantly correlated with AC (Pearson’s R = 0.69). Moisture and aeration rate were the main factors influencing TPC. The highest values for both TPC and AC were achieved in treatment 8 (60% moisture, 5 × 106 spores/mL, and 1 L/Kgwm min), resulting in a 129% and 1039% increase, respectively. Gallic acid 4-O-glucoside and geranine were identified in the PGH extracts using high-performance liquid chromatography coupled with mass spectrometry. The SSF provides eco-friendly alternatives for releasing bioactive compounds from PGH, adding value to this waste. Full article
(This article belongs to the Special Issue Microbial Biotechnological Application for Metabolite Bioprocesses)
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