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Microorganisms
Special Issues
Antibiotic Resistance in Marine-Borne Bacteria
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Antibiotic Resistance in Marine-Borne Bacteria

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Antimicrobial Agents and Resistance".

Deadline for manuscript submissions: closed (31 March 2022) | Viewed by 8824

Special Issue Editors

Dr. Anne Brisabois

E-Mail Website
Guest Editor
French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Lyon, France
Interests: foodborne pathogens; molecular characterization; antimicrobial resistance; food and seafood safety
Special Issues, Collections and Topics in MDPI journals
Dr. Jean-Yves MADEC

E-Mail Website
Guest Editor
French Agency for Food, Environmental and Occupational Health & Safety (ANSES),
Interests: antimicrobial resistance; molecular genetics and phylogenomics

Special Issue Information

Dear Colleagues,

Antimicrobial Resistance (AMR) in bacteria is a serious public health problem worldwide. It threatens the very core of modern medicine and the sustainability of an effective, global public health response to the enduring threat from infectious diseases. Systematic misuse and overuse of these drugs in human medicine and animal-food production have put every nation at risk. Tackling AMR in animals, food, and human communities is a key priority for food safety and veterinary authorities, as well as for public health authorities with a One Health global concept. Accordingly, AMR in bacteria of terrestrial origin and in human settings is routinely monitored whereas AMR data in marine-borne bacteria are rarely reported. Marine-borne bacteria not only include marine bacteria naturally carrying AMR genes but also bacteria transferred to marine environments through natural runoff from polluted lands, sewage, and more globally anthropogenic activities. In turn, food products harvested from marine environments are possible vectors for AMR transmission back to humans. Such routes of AMR transmission need deeper investigations for a more accurate risk assessment analysis. In addition, besides to the conventional phenotypic AMR determination, next-generation sequencing (NGS) approaches are now available, leading to in depth explorations of the evolution and circulation of resistant bacteria in the marine sector and their phylogenetic relationships. NGS-based methodologies also allows the tracing of the emerging AMR spread at resistome level. Global seafood production is growing each year worldwide and knowledge gaps have been pointed out on how the marine environment may contribute to the AMR threat in humans. Therefore, there is a need to determine appropriate indicator bacteria species and innovative methodologies for measuring AMR occurrence and temporal trends, and prevent the emergence of AMR bacteria in the marine sector.

 

The Special Issue aims to highlight the advances and developments regarding AMR in marine-borne bacteria considering the One Health approach. Indeed, this Special Issue will gather updated knowledge through research papers, short notes, or reviews on this subject, in order to stimulate new collaborations and new insights into research and surveillance in this field.

Thus, we will consider contributions on topics including but not limited to: 

  • Phenotypic and/or molecular description and characterization of AMR in bacteria from marine sources including seafood, marine environments, sediments, and animals
  • Description and characterization of routes of AMR transmission in this ecosystem, genetic supports, and mobile genetic elements (MGE)
  • Risk assessment of AMR in the marine sector, direct or indirect human exposure and impact in public health
  • Using new generation genomic/sequencing-based approaches, real-time and high throughput technologies for AMR genes and MGE detections, metagenomics and resistome tracing
  • Impact of anthropogenic discharges, urbanization, release of antimicrobials and other chemical contaminants or drivers of the emergence of AMR in marine-borne bacteria
  • Aquaculture practices and insights to reduce selection of AMR in bacteria in fish farming

Dr. Anne BRISABOIS
Dr. Jean-Yves MADEC
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial resistance
  • marine-borne bacteria
  • marine environment
  • seafood
  • resistome
  • AMR genes
  • mobile genetic element
  • risk assessment
  • fish marine farming

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Published Papers (3 papers)

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Research

14 pages, 1037 KiB  
Article
Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan
by Christian Xedzro, Toshi Shimamoto and Tadashi Shimamoto
Microorganisms 2023, 11(12), 2935; https://doi.org/10.3390/microorganisms11122935 - 7 Dec 2023
Cited by 3 | Viewed by 1791
Abstract
Reports have documented antimicrobial usage in aquaculture, and the aquatic ecosystem can be considered a genetic storage site for antibiotic-resistant bacteria. This study assessed the prevalence of antimicrobial resistance (AMR) among Gram-negative bacteria recovered from retail seafood in Hiroshima, Japan. A total of [...] Read more.
Reports have documented antimicrobial usage in aquaculture, and the aquatic ecosystem can be considered a genetic storage site for antibiotic-resistant bacteria. This study assessed the prevalence of antimicrobial resistance (AMR) among Gram-negative bacteria recovered from retail seafood in Hiroshima, Japan. A total of 412 bacteria were isolated and screened for the presence of β-lactamases, acquired carbapenemases, and mobile colistin-resistance (mcr) genes. Forty-five (10.9%) isolates were dominated by Morganella (28%), Proteus (22%), Aeromonas (14%), Citrobacter (8%), and Escherichia (8%) and carried AMR genes. The identified AMR genes included those encoded in integrons (19), aac(6՛)-Ib (11), blaTEM-1 (7), blaCTX-M-like (12), blaCTX-M-65 (2), blaSHV-12 (1), blaSHV-27 (1), blaOXA-10 (1), blaOXA-2 (1), and mcr (2). The most common clinical resistances were against ampicillin, colistin, sulfamethoxazole/trimethoprim, tetracycline, and ciprofloxacin. Multidrug resistance (MDR) occurred in 27 (60%) AMR isolates, and multiple antibiotic resistance indices ranged from 0.2 to 0.8. A conjugation experiment showed that 10 of the 11 selected MDR strains harbored conjugable plasmids, although PCR-based replicon typing described seven strains as untypable. IncF replicon was identified in MDR extended-spectrum β-lactamase-producing Escherichia coli of the pathogenic B2 phylogroup. Our findings suggest that retail seafood harbors MDR bacteria of human interest that require strict resistance surveillance in the seafood production continuum. Full article
(This article belongs to the Special Issue Antibiotic Resistance in Marine-Borne Bacteria)
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9 pages, 273 KiB  
Article
Prevalence and Characterization of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Enterobacterales from Tunisian Seafood
by Mehdi Sola, Yosra Mani, Estelle Saras, Antoine Drapeau, Raoudha Grami, Mahjoub Aouni, Jean-Yves Madec, Marisa Haenni and Wejdene Mansour
Microorganisms 2022, 10(7), 1364; https://doi.org/10.3390/microorganisms10071364 - 6 Jul 2022
Cited by 10 | Viewed by 2261
Abstract
Aquaculture is a rapidly expanding sector in which it is important to monitor the occurrence of multi-drug resistant (MDR) bacteria. The presence of extended-spectrum β-lactamase (ESBL-) or carbapenemase-producing Enterobacterales is a commonly used indicator of the resistance burden in a given sector. In [...] Read more.
Aquaculture is a rapidly expanding sector in which it is important to monitor the occurrence of multi-drug resistant (MDR) bacteria. The presence of extended-spectrum β-lactamase (ESBL-) or carbapenemase-producing Enterobacterales is a commonly used indicator of the resistance burden in a given sector. In this study, 641 pieces of farmed fish (sea bream and sea bass), as well as 1075 Mediterranean clams, were analyzed. All ESBL- and carbapenemase-producing Enterobacterales collected were whole-genome sequenced. The proportion of ESBL-producing Enterobacterales was 1.4% in fish and 1.6% in clams, carried by Escherichia coli (n = 23) and Klebsiella pneumoniae (n = 4). The ESBL phenotype was exclusively due to the presence of blaCTX-M genes, the most frequent one being blaCTX-M-15. The blaCTX-M-1 gene was also identified in six E. coli, among which four were carried by IncI1/pST3 plasmids, possibly betraying an animal origin. Carbapenemases were absent in fish but identified in two K. pneumoniae isolates from clams (blaNDM-1 and blaOXA-48). Several sequence types (STs) identified were associated with human MDR clones such as E. coli ST131 and ST617, or K. pneumoniae ST307 and ST147. Our results might indicate that bacteria from hospital or farm effluents can reach the open sea and contaminate seafood and fish that are living or raised nearby. Therefore, monitoring the quality of water discharged to the sea and the presence of MDR bacteria in seafood is mandatory to ensure the quality of fishery products. Full article
(This article belongs to the Special Issue Antibiotic Resistance in Marine-Borne Bacteria)
14 pages, 1945 KiB  
Article
High Throughput Screening of Antimicrobial Resistance Genes in Gram-Negative Seafood Bacteria
by Sabine Delannoy, Corine Hoffer, Raphaëlle Youf, Emilie Dauvergne, Hattie E. Webb, Thomas Brauge, Mai-Lan Tran, Graziella Midelet, Sophie A. Granier, Marisa Haenni, Patrick Fach and Anne Brisabois
Microorganisms 2022, 10(6), 1225; https://doi.org/10.3390/microorganisms10061225 - 15 Jun 2022
Cited by 12 | Viewed by 3909
Abstract
From a global view of antimicrobial resistance over different sectors, seafood and the marine environment are often considered as potential reservoirs of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs); however, there are few studies and sparse results on this sector. This [...] Read more.
From a global view of antimicrobial resistance over different sectors, seafood and the marine environment are often considered as potential reservoirs of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs); however, there are few studies and sparse results on this sector. This study aims to provide new data and insights regarding the content of resistance markers in various seafood samples and sources, and therefore the potential exposure to humans in a global One Health approach. An innovative high throughput qPCR screening was developed and validated in order to simultaneously investigate the presence of 41 ARGs and 33 MGEs including plasmid replicons, integrons, and insertion sequences in Gram-negative bacteria. Analysis of 268 seafood isolates from the bacterial microflora of cod (n = 24), shellfish (n = 66), flat fishes (n = 53), shrimp (n = 10), and horse mackerel (n = 115) show the occurrence of sul-1, ant(3″)-Ia, aph(3′)-Ia, strA, strB, dfrA1, qnrA, and blaCTX-M-9 genes in Pseudomonas spp., Providencia spp., Klebsiella spp., Proteus spp., and Shewanella spp. isolates and the presence of MGEs in all bacterial species investigated. We found that the occurrence of MGE may be associated with the seafood type and the environmental, farming, and harvest conditions. Moreover, even if MGE were detected in half of the seafood isolates investigated, association with ARG was only identified for twelve isolates. The results corroborate the hypothesis that the incidence of antimicrobial-resistant bacteria (ARB) and ARG decreases with increasing distance from potential sources of fecal contamination. This unique and original high throughput micro-array designed for the screening of ARG and MGE in Gram-negative bacteria could be easily implementable for monitoring antimicrobial resistance gene markers in diverse contexts. Full article
(This article belongs to the Special Issue Antibiotic Resistance in Marine-Borne Bacteria)
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