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Analysis of Phytochemical Components, 2nd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 January 2025 | Viewed by 3281

Special Issue Editors


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Guest Editor
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 5/2 Academician V. F. Kuprevich Street, BY-220141 Minsk, Belarus
Interests: natural products; steroids; synthesis; bioactivity; analysis; application
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Special Issue Information

Dear Colleagues,

For the whole of human civilization, plants and their components have served as an irreplaceable source of medicinal substances. Of no less importance is the role they have adopted in recent years, whereby they comprise the material basis for improving the practical properties of natural molecules through their chemical transformation. This idea has been inspiring the search for and study of new phytochemical objects for more than a century, which is gaining more and more opportunities over time thanks to the intensive development of new analytical methods. Along with the importance of the analysis of phytochemical components for medicine, its increasing role in ecology and agriculture should be noted.

Due to the necessity of overcoming the negative effects of mounting environmental problems on Earth, there is a need to develop new sensitive methods for use in the analysis of plant components. This includes ones that are present in very small quantities, but which can have a great impact on plant survival and therefore on the biosphere as a whole. Additionally, among secondary metabolites, phytochemicals are an important group of natural products from which even contemporary agriculture gets new agrochemicals and/or synthetic inspiration. As complete chemical characterization and purity checks are essential for further investigations and practical use, we invite all specialists from the field to contribute to the Special Issue, entitled “Analysis of Phytochemical Components, 2nd Edition”.

Prof. Dr. Vladimir A. Khripach
Prof. Dr. Pavel B. Drašar
Guest Editors

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Keywords

  • secondary metabolites’ analysis
  • column chromatography
  • HPLC
  • MS
  • NMR
  • analytical bio-testing
  • immunoassay analysis
  • plant metabolomics
  • separation methods
  • purity check
  • impurities analysis
  • degradation products analysis

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Published Papers (2 papers)

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Research

23 pages, 12770 KiB  
Article
Changes of Active Substances in Ganoderma lucidum during Different Growth Periods and Analysis of Their Molecular Mechanism
by Xusheng Gao, Huimin Huo, Haiying Bao, Jialu Wang and Dan Gao
Molecules 2024, 29(11), 2591; https://doi.org/10.3390/molecules29112591 - 31 May 2024
Viewed by 1257
Abstract
Ganoderma lucidum, renowned as an essential edible and medicinal mushroom in China, remains shrouded in limited understanding concerning the intrinsic mechanisms governing the accumulation of active components and potential protein expression across its diverse developmental stages. Accordingly, this study employed a meticulous [...] Read more.
Ganoderma lucidum, renowned as an essential edible and medicinal mushroom in China, remains shrouded in limited understanding concerning the intrinsic mechanisms governing the accumulation of active components and potential protein expression across its diverse developmental stages. Accordingly, this study employed a meticulous integration of metabolomics and proteomics techniques to scrutinize the dynamic alterations in metabolite accumulation and protein expression in G. lucidum throughout its growth phases. The metabolomics analysis unveiled elevated levels of triterpenoids, steroids, and polyphenolic compounds during the budding stage (BS) of mushroom growth, with prominent compounds including Diplazium and Ganoderenic acids E, H, and I, alongside key steroids such as cholesterol and 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol. Additionally, nutrients such as polysaccharides, flavonoids, and purines exhibited heightened presence during the maturation stage (FS) of ascospores. Proteomic scrutiny demonstrated the modulation of triterpenoid synthesis by the CYP450, HMGR, HMGS, and ERG protein families, all exhibiting a decline as G. lucidum progressed, except for the ARE family, which displayed an upward trajectory. Therefore, BS is recommended as the best harvesting period for G. lucidum. This investigation contributes novel insights into the holistic exploitation of G. lucidum. Full article
(This article belongs to the Special Issue Analysis of Phytochemical Components, 2nd Edition)
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16 pages, 11298 KiB  
Article
Based on HPLC and HS-GC-IMS Techniques, the Changes in the Internal Chemical Components of Schisandra chinensis (Turcz.) Baill. Fruit at Different Harvesting Periods Were Analyzed
by Bowei Sun, Yiping Yan, Mingjie Ma, Jinli Wen, Yanli He, Yining Sun, Pengqiang Yuan, Peilei Xu, Yiming Yang, Zihao Zhao, Li Cao and Wenpeng Lu
Molecules 2024, 29(8), 1893; https://doi.org/10.3390/molecules29081893 - 22 Apr 2024
Cited by 1 | Viewed by 1571
Abstract
Schisandra chinensis, as a traditional Chinese herbal medicine, has clear pharmacological effects such as treating asthma, protecting nerves and blood vessels, and having anti-inflammatory properties. Although the Schisandra chinensis fruit contain multiple active components, the lignans have been widely studied as the [...] Read more.
Schisandra chinensis, as a traditional Chinese herbal medicine, has clear pharmacological effects such as treating asthma, protecting nerves and blood vessels, and having anti-inflammatory properties. Although the Schisandra chinensis fruit contain multiple active components, the lignans have been widely studied as the primary pharmacologically active compound. The volatile chemical components of Schisandra chinensis include a large amount of terpenes, which have been proven to have broad pharmacological activities. However, when to harvest to ensure the highest accumulation of pharmacologically active components in Schisandra chinensis fruits is a critical issue. The Schisandra chinensis fruit trees in the resource nursery were all planted in 2019 and began bearing fruit in 2021. Their nutritional status and tree vigor remain consistently good. The content of lignans and organic acids in the fruits of Schisandra chinensis over seven different harvest periods was tested, and the results of high-performance liquid chromatography (HPLC) indicated that the lignan content was higher, at 35 mg/g, in late July, and the organic acid content was higher, at 72.34 mg/g, in early September. If lignans and organic acids are to be selected as raw materials for pharmacological development, the harvest can be carried out at this stage. Using HS-GC-IMS technology, a total of 67 volatile flavor substances were detected, and the fingerprint of the volatile flavor substances in the different picking periods was established. It was shown by the results that the content of volatile flavor substances was the highest in early August, and 16 flavor substances were selected by odor activity value (OAV). The variable importance in projection (VIP) values of 16 substances were further screened, and terpinolene was identified as the key volatile flavor substance that caused the aroma characteristics of Schisandra chinensis fruit at different harvesting periods. If the aroma component content of Schisandra chinensis fruit is planned to be used as raw material for development and utilization, then early August, when the aroma component content is higher, should be chosen as the time for harvest. This study provides a theoretical basis for the suitable harvesting time of Schisandra chinensis for different uses, and promotes the high-quality development of the Schisandra chinensis industry. Full article
(This article belongs to the Special Issue Analysis of Phytochemical Components, 2nd Edition)
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