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Development and Application of Atomic Spectroscopy Techniques to Biology, Health and Veterinary Research

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 3079

Special Issue Editor


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Guest Editor
Department of Chemistry, University of Surrey, Guildford, UK
Interests: analytical chemistry; atomic spectroscopy; heavy metals; metalloids; toxic elements; essential element; chemometrics

Special Issue Information

Dear Colleagues,

Analytical methods for the detection and quantification of elements in biological samples are critical for the advance of research in multiple areas of human and animal health. These include the investigation of the role of the essential role of metals and metalloids in metabolism and immune responses; the development of metal-containing drugs for more effective therapeutic treatment as well as the assessment of the impact and mechanism of action of toxic metal(loid)s in biota. The complexity of the biological matrices, and the low concentration of some of the elements present in these samples make it necessary to continue the advancements in the development and validation of accurate, precise, and very sensitive atomic spectroscopy analytical techniques.

Dr. Monica Felipe-Sotelo
Guest Editor

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Keywords

  • analytical atomic spectroscopy
  • elemental analysis
  • toxic elements
  • essential elements
  • biological samples
  • analytical method development

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Published Papers (1 paper)

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Research

12 pages, 1902 KiB  
Article
Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya
by David Krüger, Alexander Weng and Daniel Baecker
Molecules 2024, 29(1), 109; https://doi.org/10.3390/molecules29010109 - 23 Dec 2023
Cited by 2 | Viewed by 2802
Abstract
The Chinese yam (Dioscorea polystachya, DP) is known for the nutritional value of its tuber. Nevertheless, DP also has promising pharmacological properties. Compared with the tuber, the leaves of DP are still very little studied. However, it may be possible to [...] Read more.
The Chinese yam (Dioscorea polystachya, DP) is known for the nutritional value of its tuber. Nevertheless, DP also has promising pharmacological properties. Compared with the tuber, the leaves of DP are still very little studied. However, it may be possible to draw conclusions about the plant quality based on the coloration of the leaves. Magnesium, as a component of chlorophyll, seems to play a role. Therefore, the aim of this research work was to develop an atomic absorption spectrometry-based method for the analysis of magnesium (285.2125 nm) in leaf extracts of DP following the graphite furnace sub-technique. The optimization of the pyrolysis and atomization temperatures resulted in 1500 °C and 1800 °C, respectively. The general presence of flavonoids in the extracts was detected and could explain the high pyrolysis temperature due to the potential complexation of magnesium. The elaborated method had linearity in a range of 1–10 µg L−1 (R2 = 0.9975). The limits of detection and quantification amounted to 0.23 µg L−1 and 2.00 µg L−1, respectively. The characteristic mass was 0.027 pg, and the recovery was 96.7–102.0%. Finally, the method was applied to extracts prepared from differently colored leaves of DP. Similar magnesium contents were obtained for extracts made of dried and fresh leaves. It is often assumed that the yellowing of the leaves is associated with reduced magnesium content. However, the results indicated that yellow leaves are not due to lower magnesium levels. This stimulates the future analysis of DP leaves considering other essential minerals such as molybdenum or manganese. Full article
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