Plant Tissue Culture for Crop Improvement
A special issue of Methods and Protocols (ISSN 2409-9279). This special issue belongs to the section "Molecular and Cellular Biology".
Deadline for manuscript submissions: closed (20 August 2024) | Viewed by 2121
Special Issue Editor
Interests: plant tissue culture; transformation; gene editing; microscopic and analytical characterization and image-based phenotyping; trait improvement; accelerated breeding
Special Issue Information
Dear Colleagues,
Plant tissue culture and genome editing are expanding traditional plant breeding tools for crop improvement by introducing new traits more quickly and precisely. Our main objective is to advance the knowledge on plant tissue culture and genome editing tools which can be deployed efficiently to multiple crop species. This Special Issue will focus on the recent advancement in the techniques of gene editing using protoplast, cell, tissue and organ culture, and the regeneration of whole plants for improving different traits in cereals, oilseeds, pulse crops and greenhouse vegetables and fruits. Authors are invited to submit contributions in all areas related to the optimization of the promoters to drive and express Cas9 and the utilization of different fluorescent reporters and selection markers; delivery methods including electroporation, biolistic and Agrobacterium-mediated transformation and plant regeneration for achieving high-efficiency genome editing. These may include the optimization of mesophyll protoplasts system for gRNA validation and the delivery of gene editing components into haploid immature pollen. We welcome original research papers and critical reviews of recent developments in accelerated-breeding-related disciplines (prior consultation with the Guest Editor is recommended). Short research reports or case studies describing a novel DNA or protein delivery systems are also welcome.
Dr. Pankaj Bhowmik
Guest Editor
Manuscript Submission Information
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Keywords
- plant protoplasts
- microspore transformation
- guide RNA validation
- genome editing
- DNA delivery
- biolistic delivery
- grafting and plant regeneration
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