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Technology Trends in Fluorescence Detection Based on Biosensor

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 5 December 2024 | Viewed by 3063

Special Issue Editors


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Guest Editor
School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Interests: lipid membrane; bio-analysis; biosensor
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8566, Ibaraki, Japan
Interests: molecular sensors; molecular assemblies; molecular devices

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Guest Editor
School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Interests: microfluidics; lab-on-a-chip; sensor material

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Guest Editor
School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Interests: sensor development targeting tay molecular weight compounds such as drugs

Special Issue Information

Dear Colleagues,

Fluorescence-based sensors have been a growing research field for many years. Among fluorescent sensors, biosensors have progressed remarkably, and many researchers have developed superior sensors that can measure not only biological macromolecules such as proteins and nucleic acids, but also small molecules such as amino acids, sugars, and pharmaceuticals. Therefore, these are becoming an indispensable measurement technology in the fields of pharmaceuticals, agriculture, and the environment. If fluorescent probes are also considered a category of sensors, fluorescent imaging techniques using these probes are very useful for solving unknown biological processes in tissues or cells. On the other hand, sensors for on-site analysis must be usable by anyone, anywhere, at any time, and therefore measurement operation must be not only very simple but also quick. Increasing the sensitivity of these sensors is an important mission, and an approach to solving this problem has been reported using the phenomenon of fluorescence intensity enhancement through the use of inorganic metal nanomaterials. In this Special Issue, we welcome submissions of papers that introduce new principles of sensors and applications of sensors to discuss new possibilities of fluorescence-based sensors, which are making remarkable progress as described above.

Dr. Atsushi Shoji
Dr. Tsukuru Minamiki
Dr. Kazuhiro Morioka
Dr. Yukiko Moriiwa
Guest Editors

Manuscript Submission Information

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Keywords

  • on-site analysis
  • cell analysis
  • imaging
  • fluorescent protein
  • fluorescent quantum dot
  • fluorescent polymer
  • fluorescence particle
  • microlfluidics
  • lab-on-a-chip

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Published Papers (2 papers)

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Research

10 pages, 1876 KiB  
Article
Displacement Assay in a Polythiophene Sensor System Based on Supramacromolecuar Disassembly-Caused Emission Quenching
by Tsukuru Minamiki, Ryosuke Esaka and Ryoji Kurita
Sensors 2024, 24(13), 4245; https://doi.org/10.3390/s24134245 - 29 Jun 2024
Viewed by 876
Abstract
Exploring new methodologies for simple and on-demand methods of manipulating the emission and sensing ability of fluorescence sensor devices with solid-state emission molecular systems is important for realizing on-site sensing platforms. In this regard, although conjugated polymers (CPs) are some of the best [...] Read more.
Exploring new methodologies for simple and on-demand methods of manipulating the emission and sensing ability of fluorescence sensor devices with solid-state emission molecular systems is important for realizing on-site sensing platforms. In this regard, although conjugated polymers (CPs) are some of the best candidates for preparing molecular sensor devices owing to their luminescent and molecular recognition properties, the development of CP-based sensor devices is still in its early stages. In this study, we herein propose a novel strategy for preparing a chemical stimuli-responsive solid-state emission system based on supramacromolecular assembly-induced emission enhancement (SmAIEE). The system was spontaneously developed by mixing only the component polymers (i.e., polythiophene and a transient cross-linking polymer). The proposed strategy can be applied to the facile preparation of molecular sensor devices. The analyte-induced fluorescent response of polythiophene originated from the dynamic displacement of the transient cross-linker in the polythiophene ensemble and the generation of the polythiophene–analyte complex. Our successful demonstration of the spontaneous preparation of the fluorescence sensor system by mixing two component polymers could lead to the development of on-site molecular analyzers including the determination of multiple analytes. Full article
(This article belongs to the Special Issue Technology Trends in Fluorescence Detection Based on Biosensor)
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9 pages, 2941 KiB  
Communication
Near-Infrared Fluorescence Imaging Sensor with Laser Diffuser for Visualizing Photoimmunotherapy Effects under Endoscopy
by Toshihiro Takamatsu, Hideki Tanaka and Tomonori Yano
Sensors 2024, 24(5), 1487; https://doi.org/10.3390/s24051487 - 25 Feb 2024
Cited by 1 | Viewed by 1581
Abstract
The drug efficacy evaluation of tumor-selective photosensitive substances was expected to be enabled by imaging the fluorescence intensity in the tumor area. However, fluorescence observation is difficult during treatments that are performed during gastrointestinal endoscopy because of the challenges associated with including the [...] Read more.
The drug efficacy evaluation of tumor-selective photosensitive substances was expected to be enabled by imaging the fluorescence intensity in the tumor area. However, fluorescence observation is difficult during treatments that are performed during gastrointestinal endoscopy because of the challenges associated with including the fluorescence filter in the camera part. To address this issue, this study developed a device that integrates a narrow camera and a laser diffuser to enable fluorescence imaging through a forceps port. This device was employed to demonstrate that a laser diffuser with an NIR fluorescence imaging sensor could be delivered through a 3.2 mm diameter port. In addition, fluorescence images of Cetuximab-IR700 were successfully observed in two mice, and the fluorescence intensity confirmed that the fluorescence decayed within 330 s. This device is expected to have practical application as a tool to identify the optimal irradiation dose for tumor-selective photosensitive substances under endoscopy. Full article
(This article belongs to the Special Issue Technology Trends in Fluorescence Detection Based on Biosensor)
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