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Recent Advances in Enzymatic Biosensors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (1 November 2021) | Viewed by 3318

Special Issue Editor


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Guest Editor
Philipps-Universität Marburg, Institut für Pharmazeutische Chemie, Marbacher Weg 6, D-35032 Marburg, Germany
Interests: enzymatic sensors, sensor arrays, surface plasmon resonance, quantum dots, lab on a chip, biomolecular interaction analysis, medicinal plants

Special Issue Information

Dear Colleagues,

Almost 60 years ago, the first enzymatic biosensor for the detection of blood glucose was investigated. Since this time, the rapid development of different transducer techniques as well as the exploration of new applications have been observed and many of them have been applied to commercial products.

Recently, fascinating progress has been observed. A few examples are new fields of application like pharmaceutical screening and environmental analysis; the introduction of nanomaterials like nanotubes and nanoparticles; miniaturized electrode arrays; complex enzymatic systems for signal enhancement; and electro-optical biosensors. Complex arrays of semiconductor devices as well as all types of miniaturized electrodes are now the state of the art in this field. In particular, the introduction of various nanomaterials has introduced new and wide-ranging possibilities in the entire field of sensors. Moreover, a huge number of miniaturized optical sensor systems as well as hybrid sensors have been developed.

Authors are kindly invited to contribute to this Special Issue. Manuscripts should cover new applications and/or innovative sensor technologies based on one or more enzymes. All types of transducer techniques (electric devices, optical devices, magnetic devices, hybrid devices, nanoparticles, lab on a chip, etc.) are welcome, especially those which are miniaturized.

Both original research papers and review articles will be accepted.

Prof. Dr. Michael Keusgen
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • enzymatic biosensor
  • electric device
  • optical device
  • magnetic device
  • miniaturized device
  • lab on a chip
  • nanomaterials
  • nanosensor

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Published Papers (1 paper)

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Research

14 pages, 6397 KiB  
Article
Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity
by Shah Mukim Uddin, Abkar Sayad, Jianxiong Chan, Duc Hau Huynh, Efstratios Skafidas and Patrick Kwan
Sensors 2021, 21(10), 3413; https://doi.org/10.3390/s21103413 - 13 May 2021
Cited by 7 | Viewed by 2883
Abstract
HLA-B*15:02 screening before administering carbamazepine is recommended to prevent life-threatening hypersensitivity. However, the unavailability of a point-of-care device impedes this screening process. Our research group previously developed a two-step HLA-B*15:02 detection technique utilizing loop-mediated isothermal amplification (LAMP) on the tube, which requires two-stage [...] Read more.
HLA-B*15:02 screening before administering carbamazepine is recommended to prevent life-threatening hypersensitivity. However, the unavailability of a point-of-care device impedes this screening process. Our research group previously developed a two-step HLA-B*15:02 detection technique utilizing loop-mediated isothermal amplification (LAMP) on the tube, which requires two-stage device development to translate into a portable platform. Here, we report a heater-integrated lab-on-a-chip device for the LAMP amplification, which can rapidly detect HLA-B alleles colorimetrically. A gold-patterned micro-sized heater was integrated into a 3D-printed chip, allowing microfluidic pumping, valving, and incubation. The performance of the chip was tested with color dye. Then LAMP assay was conducted with human genomic DNA samples of known HLA-B genotypes in the LAMP-chip parallel with the tube assay. The LAMP-on-chip results showed a complete match with the LAMP-on-tube assay, demonstrating the detection system’s concurrence. Full article
(This article belongs to the Special Issue Recent Advances in Enzymatic Biosensors)
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