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The 10th Anniversary of Separations: Advances in Separation and...
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The 10th Anniversary of Separations: Advances in Separation and Analysis of Natural Products/Medicines

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: closed (31 December 2024) | Viewed by 4251

Special Issue Editors

Dr. Xingchu Gong

E-Mail Website
Guest Editor
Pharmaceutical Informatics Institute, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China
Interests: traditional Chinese medicine quality control; pharmaceutical engineering
Special Issues, Collections and Topics in MDPI journals
Dr. Shufang Wang

E-Mail Website
Guest Editor
Pharmaceutical Informatics Institute, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China
Interests: separation and analysis of constituents in TCM; discovery of active constituents in TCM
Dr. Guanglei Zuo

E-Mail Website
Guest Editor
Innovation Center of Translational Pharmacy, Jinhua Institute of Zhejiang University, Jinhua 321016, China
Interests: natural product chemistry; high-speed countercurrent chromatography; separation and identification; enzyme inhibitors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Natural products have been used for centuries as a source of medicine, and modern analytical techniques have enabled the identification and characterization of their active ingredients. Chromatography is a key technique for the separation and purification of these compounds, and recent advances in instrumentation and methodology have improved the efficiency and sensitivity of these processes.

The current Special Issue will focus on the latest developments in the chromatographic separation and analysis of natural products and medicines, which will cover a range of topics related to the chromatographic separation and analysis of natural products and medicines. Articles will explore the use of various chromatographic techniques, such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and high-speed counter-current chromatography (HSCCC), supercritical fluid chromatography (SFC), for the separation and purification of natural compounds. Additionally, the Issue will highlight the latest developments in sample preparation, detection methods, and separation process.

The articles in this Special Issue will be of interest to researchers and practitioners in the fields of natural product chemistry, pharmaceuticals, and analytical chemistry. They will provide valuable insights into the latest advances in chromatographic separation and analysis techniques for natural products and medicines, with potential applications in drug discovery, quality control, and clinical research.

Dr. Xingchu Gong
Dr. Shufang Wang
Dr. Guanglei Zuo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatography
  • separation process
  • natural products
  • medicinal plants
  • phytochemical analysis
  • bioactive compounds

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Further information on MDPI's Special Issue polices can be found here.

Published Papers (4 papers)

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Research

14 pages, 653 KiB  
Article
Phytochemical Study of the Plant Centaurea bruguieriana (DC.) Hand.-Mazz. subsp. belangeriana (DC.) Bornm. of the Family Asteraceae
by Kyriakos Michail Dimitriadis, Olga Tsiftsoglou, Dimitra Hadjipavlou-Litina, Mohammad Arfan and Diamanto Lazari
Separations 2024, 11(11), 319; https://doi.org/10.3390/separations11110319 - 4 Nov 2024
Viewed by 810
Abstract
The aim of this study is to isolate and identify the secondary metabolites of the aerial part of the plant Centaurea bruguieriana (DC.) Hand. -Mazz. subsp. belangeriana (DC.) Bornm. (Centaurea phyllocephala) (Asteraceae), and to study the biological activities of the extracts [...] Read more.
The aim of this study is to isolate and identify the secondary metabolites of the aerial part of the plant Centaurea bruguieriana (DC.) Hand. -Mazz. subsp. belangeriana (DC.) Bornm. (Centaurea phyllocephala) (Asteraceae), and to study the biological activities of the extracts and isolated compounds with in vitro tests. With the use of chromatography and spectroscopy we identified three elemanolides: 8α-O-(3,4-dihydroxy-2-methylenebutanoyloxy) dehydromelitensine (1), 8α-O-(3-hydroxy-4-acetoxy-2-methylene-butanoyloxy) dehydromelitensine (2) and methyl 6α,8α,15-trihydroxyelema-1,3,11(13)-trien-12-oate (3); two germacranolides: cnicin (4) and 4′-O-acetylcnicin (5); one eudesmanolide: malacitanolide (6); five flavonoids: cirsilineol (7), eupatorine (8), 5-hydroxy, 6,7,3′,4′-tetramethoxy-flavone (9), 3,4′,5,7-tetrahydroxy-6-methoxyflavone 3-O-β-D-glucopyranoside (10) and astragalin (11); and also p-OH-benzoic acid (12) and 3-hydroxy-2-methyl-butyrolactone (13). All the isolated compounds were evaluated in silico with the use of molinspiration, while the crude extract, the organic phase B and compounds 2, 4, 5 and 6 were tested as antioxidants and anti-inflammatories for the inhibition of lipid hyperoxide and the inhibition of lipoxygenase. Full article
(This article belongs to the Special Issue The 10th Anniversary of Separations: Advances in Separation and Analysis of Natural Products/Medicines)
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14 pages, 5840 KiB  
Article
Isolation, Purification, and Antioxidant Activity of Polyphenols from Cynanchum auriculatum Royle ex Wight
by Mustapha Muhammad Nasiru, Yue-E Sun, Lingyun Zhao, Taing Bunhok, Chuon Mony Roth, Sun Sovath, Hay Pharith, Weidong Wang and Chunyang Li
Separations 2024, 11(11), 316; https://doi.org/10.3390/separations11110316 - 1 Nov 2024
Cited by 1 | Viewed by 866
Abstract
Cynanchum auriculatum Royle ex Wight (CA) is a traditional medicinal and edible plant in China. This study aimed to isolate and characterize the phenolic compounds of C. auriculatum to identify its main antioxidant constituents. Polyphenols were extracted using an ultrasound-assisted ethanol extraction method, [...] Read more.
Cynanchum auriculatum Royle ex Wight (CA) is a traditional medicinal and edible plant in China. This study aimed to isolate and characterize the phenolic compounds of C. auriculatum to identify its main antioxidant constituents. Polyphenols were extracted using an ultrasound-assisted ethanol extraction method, followed by partitioning with ethyl acetate. The ethyl acetate extract was then purified through thin-layer chromatography, silica gel column chromatography, and reverse-phase silica gel column chromatography. Three monomeric compounds—cynandione A (I), 2,5-dihydroxyacetophenone (II), and radix piperacanthone (III)—were identified through their physical and chemical properties, UV and IR spectra, and liquid chromatography–mass spectrometry (LC-MS/MS). Vitamin C (VC) and 2,4-dihydroxyacetophenone were used as controls to evaluate the antioxidant potential of the two most abundant monomers. Antioxidant assays demonstrated that 2,5-dihydroxyacetophenone and cynandione A exhibited strong antioxidant activity at lower concentrations, whereas 2,4-dihydroxyacetophenone showed significantly weaker activity. Furthermore, cynandione A displayed superior cellular antioxidant activity compared to 2,5-dihydroxyacetophenone, indicating its potential as a promising bioactive compound. In conclusion, this study provides valuable insights into the phenolic composition of C. auriculatum and highlights cynandione A as a key antioxidant, paving the way for future research on its therapeutic applications. Full article
(This article belongs to the Special Issue The 10th Anniversary of Separations: Advances in Separation and Analysis of Natural Products/Medicines)
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13 pages, 2048 KiB  
Article
Separation and Characterization of Wenjin Tongluo San Essential Oil with a Comprehensive Chromatographic Separation
by Chaoyue Wang, Xionggao Han, Guanglei Zuo and Jinghui Feng
Separations 2024, 11(10), 292; https://doi.org/10.3390/separations11100292 - 11 Oct 2024
Viewed by 820
Abstract
The essential oil components of traditional Chinese medicine in-hospital preparation were complex, and one-dimensional chromatographic separation was difficult to completely separate them due to the limited peak capacity. This study was carried out to establish a comprehensive two-dimensional chromatographic separation and analysis method [...] Read more.
The essential oil components of traditional Chinese medicine in-hospital preparation were complex, and one-dimensional chromatographic separation was difficult to completely separate them due to the limited peak capacity. This study was carried out to establish a comprehensive two-dimensional chromatographic separation and analysis method based on countercurrent chromatography (CCC) and gas chromatography (GC). In this paper, we focused on the separation of the essential oil of the traditional Chinese medicine in-hospital preparation Wenjing Tongluo San by CCC × GC, and explored the orthogonality between the two chromatographic techniques to provide the new technical support for the screening of the active ingredients. A solvent system composed of n-hexane-ethyl acetate-methanol-water (9.5:0.5:8.5:1.5, v/v) was chosen for the first-dimensional CCC separation. All the fractions collected from CCC were transferred to GC for plotting two-dimensional contours map. The calculated capacity of the two-dimensional separation system exceeded 3000, which was 8 times more than that of the one-dimensional separation system. High orthogonality (r = 0.42) and spatial coverage factor (70.42%) were obtained. Meanwhile, all the fractions were identified by GC-MS. Our research provided a new methodology for separating essential oils in traditional Chinese medicine as well as an approach for evaluating the quality of traditional Chinese medicinal in-hospital preparation based on two-dimensional chromatographic fingerprints. Full article
(This article belongs to the Special Issue The 10th Anniversary of Separations: Advances in Separation and Analysis of Natural Products/Medicines)
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16 pages, 1893 KiB  
Article
Development of a QAMS Analysis Method for Industrial Lanolin Alcohol Based on the Concept of Analytical Quality by Design
by Kaidierya Abudureheman, Qinglin Wang, Hao Zhang and Xingchu Gong
Separations 2024, 11(9), 276; https://doi.org/10.3390/separations11090276 - 22 Sep 2024
Viewed by 1165
Abstract
The Analytical Quality by Design (AQbD) concept was adopted to establish a quantitative analysis of multi-components with a single marker (QAMS) method for industrial lanolin alcohol, targeting cholesterol, lanosterol, and 24,25-dihydrolanosterol. The potential critical method parameters (CMPs) were identified as column temperature, flow [...] Read more.
The Analytical Quality by Design (AQbD) concept was adopted to establish a quantitative analysis of multi-components with a single marker (QAMS) method for industrial lanolin alcohol, targeting cholesterol, lanosterol, and 24,25-dihydrolanosterol. The potential critical method parameters (CMPs) were identified as column temperature, flow rate, and gradient. Definitive screening design and statistical modeling were employed to optimize the gradient conditions of the mobile phase, column temperature, and flow rate. The Method Operable Design Region (MODR) was determined using a risk-based quantification approach. The robustness was assessed using a Plackett–Burman experimental design, followed by methodological validation. Optimal analytical conditions were as follows: acetonitrile (B)—water (A) mobile phase system; flow rate of 1.58 mL/min; detection wavelength of 205 nm; injection volume of 10 µL; and column temperature of 37 °C. A gradient elution program was implemented as follows: 0–19.0 min, 90.5% B; 19.0–25.0 min, 90.5–100% B; and 25.0–55.0 min, 100% B. Cholesterol served as an internal standard for quantifying lanosterol and 24,25-dihydrolanosterol, with relative correction factors of 0.4227 and 0.8228, respectively. This analytical method utilized only the cholesterol reference substance as an internal standard to quantify the content of cholesterol, lanosterol, and 24,25-dihydrolanosterol in industrial lanolin alcohol. It reduced the testing costs and enhanced efficiency, making it potentially suitable for widespread adoption in lanolin alcohol processing industries. Full article
(This article belongs to the Special Issue The 10th Anniversary of Separations: Advances in Separation and Analysis of Natural Products/Medicines)
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