Applications of Liquid–Liquid Chromatography

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (28 January 2021) | Viewed by 7075

Special Issue Editor


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Guest Editor
Centre ALGATECH, Institute of Microbiology, Czech Academy of Sciences, Novohradska 237 – Opatovicky mlyn, CZ 379 01 Trebon, Czech Republic
Interests: investigation and development of isolation/purification platforms using countercurrent chromatography for obtaining valuable bioproducts from the biomass of microalgae, cyanobacteria, higher plants, and edible fruits; Immunomodulatory and antioxidant properties of natural products
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Special Issue Information

Dear Colleagues,                

The identification of high-value bio-products from natural sources is a fact of wide dissemination in light of the advances in the development of increasingly efficient and sensitive analytical techniques. However, the methods developed so far to obtain these compounds from their natural sources have limitations when scaled at a larger size and the implied time- and solvent-consuming nature of the operations. Therefore, to contribute to the potential commercial use of extractable bio-products from microalga, cyanobacteria, higher plants and fruits, greater efforts are required in the fields of research and the development of isolation systems, using an efficient, cost-effective, and scalable isolation technology. In this context, countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC), two liquid–liquid chromatography techniques, have emerged as valuable alternatives due to their high efficiency and proved scalability. This Special Issue refers to the applications of CCC and CPC for obtaining valuable compounds from natural sources at the laboratory and larger scales.

Dr. José Cheel
Guest Editor

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Keywords

  • Countercurrent chromatography
  • Centrifugal partition chromatography
  • High-value products
  • Liquid–liquid separation
  • Separation technology
  • Natural products
  • Scaling-up

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Published Papers (2 papers)

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Research

21 pages, 1877 KiB  
Article
Easy Computation of the Various Topologies and Modes of Liquid–Liquid Partition Chromatography by the Theory of Random Walks
by Frédéric R. Dijoux and Tsvetelina Mandova
Separations 2021, 8(4), 41; https://doi.org/10.3390/separations8040041 - 1 Apr 2021
Viewed by 2493
Abstract
The article revisits the discrete recurrence method to model the instruments of liquid–liquid partition chromatography as counter-current chromatography (CCC) and centrifugal partition chromatography (CPC). The purpose is to simplify the computation of the concentration profiles without supplementary approximations, rather by going back to [...] Read more.
The article revisits the discrete recurrence method to model the instruments of liquid–liquid partition chromatography as counter-current chromatography (CCC) and centrifugal partition chromatography (CPC). The purpose is to simplify the computation of the concentration profiles without supplementary approximations, rather by going back to the seminal model of binomial random walks, associated with the stochastic master equation that generates simple discrete recurrence relations. It fits the model of the prototype of liquid–liquid chromatography: the Craig’s apparatus. Three emblematic separation technique group cases are computed in batch injection, batch multiple dual mode (MDM), and continuous injection by the “True Moving Bed” (TMB) in CPC. Full article
(This article belongs to the Special Issue Applications of Liquid–Liquid Chromatography)
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15 pages, 3151 KiB  
Article
Separation of the Glycosylated Carotenoid Myxoxanthophyll from Synechocystis Salina by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties
by Michaela Nováková, Tereza Fábryová, Doris Vokurková, Iva Dolečková, Jiří Kopecký, Pavel Hrouzek, Lenka Tůmová and José Cheel
Separations 2020, 7(4), 73; https://doi.org/10.3390/separations7040073 - 15 Dec 2020
Cited by 12 | Viewed by 3850
Abstract
Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In [...] Read more.
Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane–ethanol–water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health. Full article
(This article belongs to the Special Issue Applications of Liquid–Liquid Chromatography)
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