Counter-Current Chromatography in Natural Products Isolation

Abstract submission deadline

31 January 2025

Manuscript submission deadline

31 March 2025

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Topic Information

Dear Colleagues,

Counter-current chromatography (CCC) is a unique liquid–liquid partition chromatography. Without use of solids as support matrices, CCC eliminates many complications resulting from solid-supporting materials, such as irreversible absorption, constituent losses and deactivation, tailing of solute peaks and contamination. In addition, by comparison with other solid-support chromatography including silica column chromatography and reversed-phase liquid chromatography, CCC has more advantages such as a higher retention of a stationary phase, a better selection and more solvent-saving. Therefore, CCC has been widely employed for separation and preparation of natural and synthetic compounds from complex mixtures in many fields. In discussing this topic, we will focus on the separation and purification of bioactive natural products by CCC or CCC-involved separation techniques. Novel CCC instruments and separation methods developments are encouraged, and general reviews and experimental reports are welcome.

Dr. Shihua Wu
Dr. Zhi Yang
Dr. Xinyi Huang
Topic Editors

Keywords

Participating Journals

Journal Name	Impact Factor	CiteScore	Launched Year	First Decision (median)	APC
Analytica analytica	-	1.8	2020	12.8 Days	CHF 1000	Submit
Metabolites metabolites	3.4	5.7	2011	13.9 Days	CHF 2700	Submit
Molecules molecules	4.2	7.4	1996	15.1 Days	CHF 2700	Submit
Separations separations	2.5	3.0	2014	12.4 Days	CHF 2600	Submit
Toxins toxins	3.9	7.5	2009	18.9 Days	CHF 2700	Submit

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Published Papers (2 papers)

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All Analytica Metabolites Molecules Separations Toxins

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Open AccessArticle

Synthesis and Isolation of Phenol- and Thiol-Derived Epicatechin Adducts Prepared from Avocado Peel Procyanidins Using Centrifugal Partition Chromatography and the Evaluation of Their Antimicrobial and Antioxidant Activity

by Barbara Berrios-Henríquez, Matías Venegas-Toloza, María Reyes-Fuentes, Felipe Zúñiga-Arbalti, Luis Bustamante, Apolinaria García-Cancino, Julio Alarcón-Enos and Edgar Pastene-Navarrete

Molecules 2024, 29(12), 2872; https://doi.org/10.3390/molecules29122872 - 17 Jun 2024

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Abstract

Polyphenols from agro-food waste represent a valuable source of bioactive molecules that can be recovered to be used for their functional properties. Another option is to use them as starting material to generate molecules with new and better properties through semi-synthesis. A proanthocyanidin-rich [...] Read more.

Polyphenols from agro-food waste represent a valuable source of bioactive molecules that can be recovered to be used for their functional properties. Another option is to use them as starting material to generate molecules with new and better properties through semi-synthesis. A proanthocyanidin-rich (PACs) extract from avocado peels was used to prepare several semi-synthetic derivatives of epicatechin by acid cleavage in the presence of phenol and thiol nucleophiles. The adducts formed by this reaction were successfully purified using one-step centrifugal partition chromatography (CPC) and identified by chromatographic and spectroscopic methods. The nine derivatives showed a concentration-dependent free radical scavenging activity in the DPPH assay. All compounds were also tested against a panel of pathogenic bacterial strains formed by Listeria monocytogenes (ATCC 7644 and 19115), Staphylococcus aureus (ATCC 9144), Escherichia coli (ATCC 11775 and 25922), and Salmonella enterica (ATCC 13076). In addition, adducts were tested against two no-pathogenic strains, Limosilactobacillus fermentum UCO-979C and Lacticaseibacillus rhamnosus UCO-25A. Overall, thiol-derived adducts displayed antimicrobial properties and, in some specific cases, inhibited biofilm formation, particularly in Listeria monocytogenes (ATCC 7644). Interestingly, phenolic adducts were inactive against all the strains and could not inhibit its biofilm formation. Moreover, depending on the structure, in specific cases, biofilm formation was strongly promoted. These findings contribute to demonstrating that CPC is a powerful tool to isolate new semi-synthetic molecules using avocado peels as starting material for PACc extraction. These compounds represent new lead molecules with antioxidant and antimicrobial activity. Full article

(This article belongs to the Topic Counter-Current Chromatography in Natural Products Isolation)

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Open AccessArticle

Bioactivity-Guided High Performance Counter-Current Chromatography and Following Semi-Preparative Liquid Chromatography Method for Rapid Isolation of Anti-Inflammatory Lignins from Dai Medicinal Plant, Zanthoxylum acanthopodium var. timbor

by Qing-Fei Fan, Lan Zhou, Pian-Chou Gongpan, Chuan-Li Lu, Hua Chang and Xun Xiang

Molecules 2023, 28(6), 2592; https://doi.org/10.3390/molecules28062592 - 13 Mar 2023

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Abstract

The development of Dai medicine is relatively slow, and Zanthoxylum has great economic and medicinal value. It is still difficult to obtain medicinal components from the low-polarity parts of Zanthoxylum belonging to Dai medicine. In this study, we introduced one simple and quick [...] Read more.

The development of Dai medicine is relatively slow, and Zanthoxylum has great economic and medicinal value. It is still difficult to obtain medicinal components from the low-polarity parts of Zanthoxylum belonging to Dai medicine. In this study, we introduced one simple and quick strategy of separating target compounds from the barks of Z. acanthopodium var. timbor by high-performance countercurrent chromatography (HPCCC) with an off-line anti-inflammatory activity screening mode. The development of this strategy was based on the TLC-based generally useful estimation of solvent systems (GUESS) method and HPCCC in combination. This paper presented a rapid method for obtaining target anti-inflammatory compounds. Three lignins were enriched by HPCCC with an off-line inhibition mode of nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophage cells, using petroleum ether–ethyl acetate–methanol–water (3:2:3:2) as the solvent system. The results showed that this method was simple and practical and could be applied to trace the anti-inflammatory components of the low-polarity part in Dai medicine. Full article

(This article belongs to the Topic Counter-Current Chromatography in Natural Products Isolation)

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