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Article

Phytochemical Composition and Antioxidant Capacity of Seven Saskatoon Berry (Amelanchier alnifolia Nutt.) Genotypes Grown in Poland

by
Sabina Lachowicz
1,*,
Jan Oszmiański
1,
Łukasz Seliga
2 and
Stanisław Pluta
2
1
Department of Fruit, Vegetables and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Science, 37, Chełmońskiego Street, 51-630 Wrocław, Poland
2
Department of Horticultural Crop Breeding, Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
*
Author to whom correspondence should be addressed.
Molecules 2017, 22(5), 853; https://doi.org/10.3390/molecules22050853
Submission received: 5 April 2017 / Revised: 18 May 2017 / Accepted: 18 May 2017 / Published: 21 May 2017
(This article belongs to the Section Natural Products Chemistry)
Browse Figures
Versions Notes

Abstract

:
The basic chemical composition, bioactive compounds, and antioxidant capacity of fruits of three new Polish breeding clones (No. 5/6, type S, and type N) and four Canadian cultivars (cvs.) (“Martin”, “Smoky”, “Pembina”, and “Honeywood”) grown in Poland in 2016 were investigated. Fruits were analyzed for their contents of triterpenoids, carotenoids, chlorophylls, and polyphenolics with the ultra-performance liquid chromatography photodiode detector-quadrupole/time-of-flight mass spectrometry (UPLC-PDA-Q/TOF-MS) method, sugar with the high-performance liquid chromatography–evaporative light scattering detector (HPLC-ELSD) method, and antioxidant capacity with the ability to reduce free radical (ABTS) and ferric reducing ability of plasma (FRAP) method. Thirty-eight bioactive compounds, including twenty-eight polyphenolic compounds (four anthocyanins, nine phenolic acids, nine flavonols, and seven flavan-3-ols), four carotenoids, two chlorophylls, and three triterpenoids were identified in the fruits. The fruits of the tested Saskatoon berry genotypes were found to be rich in phenolic compounds (3773.94–6390.36 mg/100 g·dm), triterpenoids (66.55–91.31 mg/kg·dm), and carotenoids (478.62–561.57 mg/kg·dm), with high ABTS and FRAP capacity (10.38–34.49 and 9.66–25.34 mmol·Trolox/100 g·dm, respectively). Additionally, the berries of these genotypes seemed to be a good source of sugar (9.02–19.69 g/100 g), pectins (0.67%–1.33%), and ash (0.59%–0.67%). Some genotypes of Saskatoon berry, especially the clones type S, type N, and cvs. “Honeywood” and “Smoky”, may be selected for their potential applications in commercial cultivation to produce fruits with valuable health-promoting nutritional effects on human health. Additionally, three new genotypes that may offer new functional materials can be recommended for fruit growers.

1. Introduction

In North America, the species Amelanchier alnifolia Nutt., called the Saskatoon berry, has been grown and harvested commercially for many years. The fruit is a berry-like pome, red to purple to nearly black at maturity, 5–15 mm in diameter, insipid to delectably sweet, maturing at the end of June/beginning of July [1,2,3,4]. The fruits are good to eat raw, tasting somewhat like a blueberry, strongly accented by the almond-like flavor of the seeds. Saskatoon berry is a good source of typical health-promoting nutrients, and can constitute a suitable supplement for modern human nutrition. The berries contain numerous health-beneficial phytochemical compounds, mainly phenolic acids, flavonols, anthocyanins, carotenoids, minerals (manganese, calcium, potassium, magnesium, iron), vitamins (tocopherol, pyridoxine, riboflavin, ascorbic acid, riboflavin, thiamin, pantothenic acid), and pectin. In Canada, the fruits are harvested locally for pies and jams, and are very suitable for the production of various processed products. Because of the fruit’s composition, mainly concerning the content of bioactive compounds, especially polyphenols, the Saskatoon berry can play a very beneficial antioxidant, anti-inflammatory, antitumor, hypoglycemic, antidiabetic, and antiradical role, as well as regulating the level of glucose and glycogen accumulation in the human diet and has high nutritional and nutraceutical value [3,5,6,7,8,9,10]. The fruit quality and nutraceutical value of the Saskatoon berry are comparable to those of the fruit of the high-bush blueberry (Vaccinium corymbosum L.), but cultivation of Amelanchier alnifolia is much easier, because it is less demanding in terms of soil quality and weather conditions.
Poland has a long tradition as a major fruit producer and exporter of various berry crops, such as strawberry, raspberry, red and blackcurrants, gooseberry, chokeberry, and high-bush blueberry. The fruit production of most of these crops has been increasing in our country. To improve the profitability and competitiveness of small fruit production, Polish growers are interested in growing new crops, such as Saskatoon berry. The fruits of this species have unique chemical composition, taste, and usefulness both for consumers of fresh fruit and for the processing and food industry. They can be used to make drinks, jams, jellies, fruit pies, and liquors. The fruit of this crop can also be mixed with other fruits, especially those with a sour taste. Interest in the cultivation of this species is also stimulated by the natural high winter hardiness level of its plants. It means that it can be grown under the environmental conditions that exist in Poland. So far, the Saskatoon berry (A. alnifolia) has been cultivated on a small scale in Poland, with plans for increased fruit production in the near future [11,12].
Therefore, the aim of this study was to determine and compare polyphenols, carotenoids, chlorophylls, and triterpenoids by UPLC-PDA-Q/TOF-MS, and antioxidant capacity (the ability to reduce free radical (ABTS) and ferric reducing ability of plasma (FRAP)) in fruits of seven Saskatoon genotypes grown in a trial in Poland. In addition, the chemical properties of the fruits, such as ash, dry weight, sugar, titratable acidity, and pectin content were evaluated. Moreover, the characterization of phytochemicals and antioxidant capacity presented in this paper could offer new functional materials recommended for fruit growers and also useful for selecting the Saskatoon berry genotypes with a high content of bioactive compounds in their fruits used by the food processing industry and for the production of health beneficial products.

2. Results and Discussion

2.1. Major Chemical Compounds

The analytical results of fruit for dry weight, titratable acidity, pH, pectins, ash, and sugars of the tested Saskatoon berry genotypes are given in Table 1. Significant differences (p < 0.05) were revealed for the investigated basic chemical parameters among all the genotypes grown in Poland.
The average dry matter content in the fruits of the analyzed Saskatoon genotypes was 23.18 g/100 g and varied from 19.11 for clone No. 5/6 to 29.59 g/100 g for cv. “Pembina”. These results were comparable to those obtained in fruits of the Saskatoon berry cultivars grown in the Czech Republic and Canada [8,13,14]. The average value of pH of the fruits of analyzed genotypes was 3.70; the lowest value (3.02) was observed for cv. “Pembina” and the highest (3.95, 3.99) for cv. “Smoky” and clone No. 5/6, respectively. The titratable acidity and the ratio of sugar (soluble solids) to acid contents are two important determinants of fruit taste and consumer’s acceptability. The total titratable acidity in different genotypes, expressed as g/100 g of citric acid, varied from 0.37 to 0.64 g/100 g for the clones type S and No. 5/6, respectively. According to Mazza [3], the content of titratable acidity in the fruits of cv. “Smoky” was 3.12 g/100 g.
Mineral composition of the fruits depends not only on the genotypes, but also on the growing conditions [15,16]. The average content of ash in the analyzed genotypes was 0.91%, and it ranged from 0.61 to 1.14% for clone No. 5/6 and clone type S, respectively. Our results showed similar content of ash in the Saskatoon berry of tested cultivars in Canada [3]. Pectin is one form of soluble fiber responsible for the prevention of diabetes, obesity, and cardiovascular disease [17]. The average pectin amount in fruits of the tested genotypes was 0.93%; the lowest content (0.67%) was observed in cv. “Pembina” and the highest one (1.33%) in clone type S.
Fructose, sorbitol, glucose, and sucrose were the main sugars analyzed in the Saskatoon berry genotypes. The content of total sugar in the fruits of different genotypes grown in Poland ranged from 9.02 to 19.69 mg/100 g. The lowest value of this component was determined in the fruits of clone No. 5/6. The highest amount of total sugars was obtained in the fruits of another clone, type S. This content was more than double that of the lowest one observed in clone No. 5/6. The average content of total sugar in the studied genotypes was 14.78 mg/100 g, and it was 21% higher than in the Saskatoon berry genotypes evaluated in Canada [3]. In the fruits of our genotypes, fructose and glucose were the dominant sugars (31%–47% and 24%–50%, respectively; Table 1). Fruits of Canadian cv. “Martin” had the lowest glucose content (2.50 g/100 g), and clone type S had the highest one (8.76 g/100 g). The content of fructose varied from 3.14 g/100 g for clone No. 5/6 to 7.29 g/100 g for clone type S. The average fruit content of fructose, glucose, and sucrose in the analyzed genotypes was 5.47, 6.38, and 0.59 g/100 g, respectively. It was 8% lower, and 18% and 71% higher than the content of these sugars in the fruit of the Saskatoon berry genotypes analyzed by Mazza [3]. Fructose and glucose are known to be sweeter than sucrose; therefore these sugars determined the sweetness of fruits of the Saskatoon berry.

2.2. Identification and Quantification of Phenolic Compounds

Identification and quantification of 29 compounds belonging to anthocyanins, phenolic acids, flavonols, and flavan-3-ols was based on a comparison of their retention times and ions at m/z (MS) and fragment at m/z (MS/MS) data with available standards and published data. The identification and concentration results are presented in Table 2 and Figure 1. The structures of these compounds were identified by comparison of their spectral and MS and/or MS/MS data to those reported in previous studies [9,18,19,20,21].
The polyphenolic compounds mostly contributed to the antioxidant activity of Saskatoon berries. The high content of the polyphenol antioxidants in the fruit is responsible for the observed antidiabetic, anti-inflammatory, and chemo-protective effects [10]. The concentration of the phenolic compounds, based on the UPLC-PDA-FL analysis, in Canadian cultivars (“Smokey”, “Martin”, “Pembina”, “Honeywood”) and breeding clones (No. 5/6, type N, type S) was significantly different in our studies. The average main group of phenolic compounds in fruits of the analyzed genotypes were as follows: flavan-3-ols (~46.0%) > anthocyanins (~30.0%) > phenolic acids (~18%) ≥ flavonols (~4%). The average content of phenolic compounds in the fruits of the genotypes grown in Poland was 4946.14 mg/100 g·dm. It ranged from 2992.35 to 6390.36 mg/100 g·dm for cv. “Pembina” and breeding clone type S, respectively. The highest levels of phenolic compounds (>5500 mg/100 g·dm) were found in the fruits of cvs. ”Honeywood” and ”Smoky” and breeding clones type S and type N, but the lowest content of phenolic compounds (<3500 mg/100 g·dm) was observed in clone No. 5/6 and cv. ”Pembina”. According to Lavola et al. [18], the content of phenolic compounds in fruits of cvs. ”Smoky” and ”Honeywood” grown in Finland was 50% and 38% lower than in both cultivars in our studies. However, in the study of Rop et al. [8], the fruit content of phenolic compounds in cvs. ”Smoky” and ”Martin” in the Czech Republic was 46% and 18% lower than in the same cultivars grown in Poland. The phenolic compounds’ concentration in our study was comparable to those reported in different Saskatoon berry cultivars [19,20], lower than chokeberry (Aronia melanocarpa) [22], and higher than cranberry (Vaccinium macrocarpon) fruits [23]. The phenolic compounds in fruit crops depend on many factors, such as the environmental and climatic conditions during plant growth, the place of cultivation and agricultural practices, the extraction process of fruits, the solvent, and the methodology used to identify the compounds [10,24].

2.2.1. Anthocyanins

The red to purple color of the Saskatoon berry comes mainly from anthocyanins. The main fraction of polyphenols responsible for their health-promoting activities, in addition to proanthocyanidins, is anthocyanins. Anthocyanin-rich extracts have shown both anti-inflammatory effects and the induction of TNF-α [10]. The average content of total anthocyanins in the seven Saskatoon berry cultivars and clones was 1504.88 mg/100 g·dm and depended significantly on the genotype. The concentrations of anthocyanins in the fruits of analyzed genotypes ranged from 2123.43 to 798.22 mg/100 g·dm. High levels of anthocyanins (>1500 mg/100 g·dm) were found in cvs. “Honeywood” and “Smoky” and breeding clones type S and type N. The lowest level (<900 mg/100 g·dm) of anthocyanins was obtained in clone No. 5/6 and cv. ”Pembina”. According to Lavola et al. [18], the fruit content of anthocyanins in cvs. “Smoky” and “Honeywood” grown in Finland was 32% lower and 14% higher than in both cultivars in Polish conditions. In studies by Hosseinian and Beta [25], the concentration of anthocyanins was similar to our results in cv. “Smoky” and 35% higher than cv. “Martin”. The fruit content of anthocyanins of cv. “Honeywood” grown in Finland and Canada was 28% higher and 23% lower than cv. “Honeywood” grown in Poland. In our studies, the content of anthocyanins in fruits of cv. “Smoky” was 29% higher than in this cultivar grown in Canada [26]. According to Jin et al. [21], the contents of anthocyanins in cvs. “Pembina” and “Honeywood” were 50% and 31% lower than in both Saskatoon berry cultivars grown in Poland. The main anthocyanin compound in berries of seven analyzed genotypes was cyanidin-3-O-galactoside (57% of total anthocyanins). In the results of Lavola et al. [18] and Ozga et al. [20], cyanidin-3-O-galactoside was also a major compound (around 60%) of all the anthocyanins in fruits. The content of anthocyanins and their composition in the different Saskatoon berry genotypes depend on environmental factors, growing location, climatic conditions, genetic traits, cultivar, as well as on the exposure of fruits to light during the ripening of fruits [18].

2.2.2. Phenolic Acids

The concentration of phenolic acids in the tested genotypes of the Saskatoon berry ranged from 564.70 to 1216.92 mg/100 g·dm; the average content was 887.64 mg/100 g·dm (Table 2). The highest levels of phenolic acids (>1100 mg/100 g·dm) were found in fruits of cv. “Honeywood” and breeding clone type S, but cvs. “Martin” and “Pembina‘’ had the lowest one (<600 mg/100 g·dm). In the fruits of all the Saskatoon genotypes grown in Poland, the chlorogenic and neochlorogenic acids were major components (45–72% and 14–22%, respectively, of the total phenolic acids). It is known that the phenolic acids, especially chlorogenic acid, have potential antioxidant activity, inhibit DNA damage, and increase the resistance of low-density lipoprotein to oxidation, and they are precursors of the flavor in fruits and vegetables [22]. According to Lavola et al. [18], chlorogenic acid was also a major compound of all the phenolic acids in different Saskatoon cultivars grown in Finland (constituting 49%, 52%, and 42% in cvs. “Smoky”, “Thiessen”, and “Honeywood”, respectively). In research by Jin et al. [21], the content of phenolic acids in the fruits of cvs. “Pembina” and “Honeywood” was 67% and 21% lower than in both cultivars analyzed in our studies.

2.2.3. Flavonols

Flavonols were the smallest group of phenolic compounds found in the fruits of the Saskatoon berry (Table 2). The concentration of flavonols in fruits of the tested genotypes ranged from 141.20 to 400.91 mg/100 g·dm; the average content was 288.87 mg/100 g·dm. The lowest value of flavonols was found in the fruits of cv. “Pembina” and the highest content in the breeding clone type S. The concentrations of these compounds in the analyzed Saskatoon berry genotypes in our study were similar to those obtained in Finland and Canada [18,20]. According to Rop et al. [8], the content of flavonols in cvs. “Smoky” and “Martin” grown in the Czech Republic was 30% and 59% higher than for the same cultivars grown in Polish conditions. The main flavonol compound in berries of the seven analyzed genotypes was quercetin-3-O-galactoside (around 60% of the total flavonols). In the results of Lavola et al. [18], quercetin-3-O-galactoside was also a major compound in the fruits.
Flavonols, especially quercetin derivatives, are implicated in the berry’s health benefits, highlighted mainly for their excellent antioxidant activity. Quercetin is active in several diseases, such as cancer, neurodegenerative pathologies, and cardiovascular disease [27].

2.2.4. Flavan-3-ols

Flavan-3-ols consisting of oligomers and polymeric procyanidins were the major group of the Saskatoon berry polyphenolic compounds. Flavan-3-ols are compounds that influence lipid metabolism and regulate the level of glucose and glycogen accumulation. The average content of total flavan-3-ols in the fruits of the tested genotypes was 2264.74 mg/100 g·dm. The highest (2782.24 mg/100 g·dm) concentration of total flavan-3-ols was determined in the fruits of cv. “Honeywood”, and the lowest (1395.30 mg/100 g·dm) concentration in clone No. 5/6. The major group of total flavan-3-ols in all Saskatoon genotypes grown in Poland was polymeric procyanidins (74–93%). The total content of polymeric procyanidins in analyzed genotypes ranged from 1189.76 to 2631.73 mg/100 g·dm for clone No. 5/6 and type S, respectively. The highest levels of polymeric procyanidins (>2000 mg/100 g·dm) were found in the fruits of cvs. “Honeywood” and “Smoky” and breeding clones type S and type N. The lowest (<1400 mg/100 g·dm) content of polymeric procyanidins was observed in fruits of clone No. 5/6 and cv. “Pembina”. The concentration of the monomer and dimer of flavan-3-ols in the tested genotypes of the Saskatoon berry ranged from 180 in clone type S to 444.55 mg/100 g in cv. “Pembina”. As reported by Lavola et al. [18], the varying content of flavan-3-ols in the different Saskatoon berry genotypes depends on environmental factors, climatic conditions, growing location, cultivar, and genetic traits. From the literature data, it follows that among the berries, substantial amounts of flavan-3-ols are found in chokeberries, Saskatoon berries, lingonberries, blueberries, and cranberries [10]. The flavan-3-ols concentration in our study was comparable to the values reported in different Saskatoon berry cultivars [19,20], lower than chokeberry [22], and higher than cranberry and blueberry [10,23].

2.3. Triterpenoid Compounds

Table 3 shows the data after identification and quantification of the triterpenoids in the fruits of seven genotypes of Saskatoon berry. The detected compounds were identified as betulinic, ursolic, and oleanolic acids based on their molecular ion [M − H]− at m/z 455.3, MS profiles with the fragmentation pathways, UV-Vis spectra, and the retention times (Rt) of authentic standards. Triterpenoids have not been found in Saskatoon berries so far.
The total triterpenoid compound content in the Saskatoon berry varied depending on the evaluated genotype (Table 3). In our studies, the total triterpenoid compound content in the analyzed genotypes of the Saskatoon berry ranged from 72.21 to 91.31 mg/kg·dm for breeding clones No. 5/6 and type S, respectively. The average content of total triterpenoid compounds in the fruit of the analyzed genotypes was 79.0 mg/kg·dm. The major compound was ursolic acid (~84% of total triterpenoid compound), followed by betulinic and oleanolic acid (~12% and 5%), respectively. The total triterpenoid compound is dependent on many factors, such as climate, environmental conditions, and the degree of fruit maturity at the harvest. Triterpenoids are mainly located in cuticular waxes and exhibit many biological activities, such as antioxidant, anticancer, anti-inflammatory, and antifungal properties [28,29]. The major total triterpenoid compound in cranberry (Vaccinium macrocarpon) (20%), apple (Malus domestica) (98%), and sweet cherry (Prunus avium) (60% of all wax extract) was ursolic acid, as in different genotypes of the Saskatoon berry [29]. Jetter et al. [30] reported various compositions and contents of triterpenoids in the cuticular wax of many plant species. According to Szakiel et al. [31], the concentration of oleanolic (50%) and ursolic acid (28%) in bilberry (Vaccinium myrtillus) fruit from Finland was higher than in the Saskatoon berry. The major compounds of the total triterpenoid compound in the bilberry fruit were oleanolic and ursolic acid.

2.4. Composition of Carotenoids and Chlorophylls

Table 3 show the data after the identification and quantification of carotenoids and chlorophylls in seven genotypes of Saskatoon berry using UPLC-PDA-Q/TOF-MS experiments, along with their retention times, UV-Vis spectral profiles at 200–800 nm, and a comparison with standard reference compounds, when available.
Compound 1 was identified as zeaxanthin (Table 3) based on its [M + H]+ ion at 601.0521, which is consistent with the data reported in the literature for this compound [13]. Compound 2 was identified as all-trans-lutein, based on its ion at m/z 568.0021 and on the resulting product ions at m/z 551 and 553, which is consistent with the data reported in the literature for this compound [24], and with the authentic standard. Compound 3 with an identical molecular ion and fragmentation pathway was identified as 13-cis-lutein. This compound had absorption maxima at 335, 420, 446, and 474 nm, which is characteristic for this compound [24]. Compound 4 had molecular ions at m/z 537.1451 that fragmented at m/z 445. This compound was identified as β-carotene [13].
The total carotenoid content in the Saskatoon berry varied depending on the evaluated genotype (Table 3). The carotenoid content concentration in the analyzed genotypes ranged from 478.62 mg/kg·dm for cv. “Pembina” to 561.57 mg/kg·dm for clone type S. The average content of carotenoid content in seven Saskatoon berry genotypes grown in Poland was 514.44 mg/kg·dm. The major compounds in fruits of the Saskatoon berry were β-carotene (~68% of all carotenoid content) and lutein (~32%), followed by zeaxanthin (~0.7%). According to Mazza and Cottrell [13], the main carotenoid in fruits of cv. “Honeywood” Saskatoon berries was also β-carotene (~42% of total carotenoids), followed by lutein (~37%) and zeaxanthin (~20%).
Compound 1 was identified as chlorophyllide b due to its [M + H]+ ion at m/z 907.1181 and the resulting product ion at m/z 629, which is consistent with the data reported in the literature for this compound [24] and with the authentic standard. Compound 2, its [M + H]+ ion at m/z 871.1362, and the resulting product ion at m/z 593, 533, was identified as pheophytin a, in agreement with the literature data [24].
The average concentration of chlorophylls in the analyzed genotypes was 92.51 mg/kg·dm and depended significantly on the genotypes (Table 3). The content of chlorophylls in the different Saskatoon berry genotypes grown in Poland ranged from 71.2 mg/kg·dm for cv. “Smoky” to 115.15 mg/kg·dm for clone No. 5/6. It is known that the chlorophyll compounds are present mainly in the fruit and vegetable skins. In general, the fruits of all analyzed Saskatoon genotypes were characterized by low concentrations of chlorophylls. Perhaps it can be explained by the fact that the freeze-drying and analysis were conducted using fully ripe Saskatoon berries. This hypothesis was corroborated by Pumilia et al. [32], who reported that a low amount of chlorophylls is indicative of the fruit ripeness. Moreover, the green color of the fruit disappears, and the red color emerges in the ripening process of the fruits, derived mainly from anthocyanins. In addition, carotenoids are insoluble in water and occur mainly in the fruit and vegetable skin [33].

2.5. Antioxidant Activity and Correlations

The results of the antioxidant capacity of the tested Saskatoon berry genotypes measured by the ABTS and FRAP methods are presented in Figure 2. Significant differences in the antioxidant capacity were observed between the analyzed genotypes using these assays. The average result of the antioxidant capacity (the ability to reduce free radical (ABTS) and ferric reducing ability of plasma (FRAP)) assay in fruits was 24.15 and 17.90 mmol·Trolox/100 g·dm, respectively. The highest antioxidant capacity (measured by both methods) in the tested genotypes was found in the Polish clone type S (34.49 and 25.34 mmol·Trolox/100 g·dm), while the lowest value was determined in the fruits of cv. “Pembina” (10.38 and 9.66 mmol·Trolox/100 g·dm). The antioxidant capacity in cv. “Smoky” grown in Poland, analyzed by the ABTS assay, was 60% lower than this cultivar grown in Canada [34]. According to Bakowska-Barczak and Kolodziejczyk [9], the antioxidant capacity analyzed by the ABTS assay was 9% lower than the value of the antioxidant capacity of the Saskatoon berry cv. “Smoky” and 9% higher than cvs. “Martin” and “Pembina”.
The correlations between the antioxidant capacity and selected phytochemicals were also examined in the studies (Table 4). The antioxidant capacity (ABTS and FRAP assay) of the analyzed genotypes of the Saskatoon berry showed the strongest correlation with the content of phenolic compounds (r2 = 0.925 for the ABTS and 0.900 for the FRAP assay). Furthermore, a significant, strong correlation was found between phenolic compounds and antioxidant capacity. Bakowska-Barczak and Kołodziejczyk [9] reported similar results for cv. “Honeywood” and stated that the antioxidant capacity correlated strongly with the anthocyanins and phenolic compounds. The type of phenolic compounds also played a very important role in antioxidant activity. Positive correlations were found between anthocyanins and polymeric procyanidinsand the results of the antioxidant capacity. Additionally, the clones type S and type N and cvs. “Honeywood” and “Smoky” high anti-radical activity was attributed to the presence of anthocyanins and polymeric procyanidins. Most carotenoids have a system of conjugated double bonds, which influence the antioxidant capacity. The antioxidant capacity of carotenoid compounds is used mainly in the scavenging of free radicals and the reduction of the risk of developing degenerative diseases. Chlorophyll has antioxidant, antimutagenic, and anticancer properties [24]. Despite the high reactivity of carotenoids and chlorophylls, positive correlations were found between carotenoid compounds, β-carotene, and lutein, and a negative correlation between chlorophylls and antioxidant capacity. Also triterpenoids, due to their antioxidative properties, exhibit anti-HIV activity and showed significant cytotoxicity in leukemia cells as well as anticancer, antioxidative, and anti-inflammatory properties, and a significant positive correlation was found between triterpenoid compounds and the antioxidant capacity [28,29]. In the studies of Loza-Mejía and Salazar [28] and Szakiel et al. [29], the antioxidant capacity of the fruit was found to be strongly and positively correlated with the triterpenoids compound (p = 0.05, r2 = 0.621 for ABTS and r2 = 0.607 for FRAP). Therefore, it could be concluded that the antioxidant capacity of the tested Saskatoon genotypes correlated with the levels of the phenolic compounds, anthocyanins, flavonols, procyanidins polymeric, carotenoid compounds, and triterpenoid compounds. Moreover, the antioxidant capacity of the Saskatoon berry depended on the chemical composition of the antioxidant compounds [8].

2.6. Principal Component Analysis (PCA)

The average results of our studies obtained for seven different genotypes (three Polish clones No. 5/6, type S, and type N, and four Canadian cultivars “Martin”, “Smoky”, “Pembina”, and “Honeywood”) of the Saskatoon berry in their bioactive composition and the antioxidant activity using principal component analysis (PCA) are presented in Figure 3. Two main PCAs for the analysis of the seven genotypes grown in Poland accounted for 82.60% of the total variability, PC1 for 71.60% and PC2 for 9.00% (Figure 3). The results obtained from PCA using the linkage method among the groups indicated the presence of four clusters:
(1)
clones type S and type N with the highest concentrations of oleanolic acid (OA), flavonols (FL), phenolic acids (PA), total carotenoid compounds (TCC), β-carotene (BCA), total triterpenoid compounds (TTC), and zeaxanthin. Additionally, a positive correlation with antioxidant capacity was detected.
(2)
cvs. “Smoky” and “Honeywood” with the high antioxidant activity (ABTS, FRAP), and high contents of anthocyanins (ANT), phenolic compounds (PC), betulinic acid (BA), ursolic acid (UA), polymeric procyanidin (PP), total sugar (TS), ash, and lutein. Additionally, a positive correlation with antioxidant capacity was detected.
(3)
cvs. “Martin” and “Pembina” with high contents of flavan-3-ols (F3o) and a positive correlation with antioxidant capacity.
(4)
clone No. 5/6 with the highest content of chlorophylls (CH) and low antioxidant capacity.

3. Materials and Methods

3.1. Reagent and Standard

Acetonitrile, formic acid, methanol, all-trans-β-carotene, α-carotene, all-trans-lutein, neoxanthin, violaxanthin, antheraxanthin, β-cryptoxanthin, chlorophyll a, betulinic, oleanolic and ursolic acid, ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), methanol acetic acid, and phloroglucinol were purchased from Sigma-Aldrich (Steinheim, Germany). (−)-Epicatechin, (+)-catechin, procyanidin A2 and B2 chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, di-caffeic quinic acid, p-coumaric acid, 4-caffeoylquinic, kampferol-3-galactoside, quercetin-3-O-rutinoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-O-arabinoside, quercetin-3-O-xyloside, quercetin-deoxyhexo-hexoside caffeic acid, cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, and cyanidin-3-O-xyloside were purchased from Extrasynthese (Lyon, France). Acetonitrile for ultra-phase liquid chromatography (UPLC; Gradient grade) and ascorbic acid were purchased from Merck (Darmstadt, Germany).

3.2. Plant Materials

Fruits of the Saskatoon berry (A. alnifolia) of seven genotypes (three Polish clones No. 5/6, type S, and type N, and four Canadian cultivars “Martin”, “Smoky”, “Pembina”, and “Honeywood”) were used in the study. Fruit samples (~1.0 kg each) of six genotypes were collected from bushes grown in a field, belonging to the Research Institute of Horticulture in Skierniewice, Central Poland (51°55′24″ N, 020°5′58″ E). The one fruit sample of Canadian cultivar “Honeywood” was collected from the horticultural farm in Wojciechów near Lublin, Poland (51°14′08″ N 22°14′41″ E). The plant material for the field experiment was derived from vegetative propagation in an in vitro culture in a tissue culture laboratory at the Research Institute of Horticulture in Skierniewice. Four Canadian cultivars of the Saskatoon berry (Amelanchier alnifolia Nutt.), such as “Martin”, “Smoky”, “Pembina”, and “Honeywood” were imported from Canada’s tissue culture laboratory in 2007 as multiply (“Multiplat”) potted plants (“72 Cell Liner plugs”). Three Polish breeding clones No. 5/6, type S, and type N were selected by Prof. Stanisław Pluta in 2007/2008 at the Plant Breeding Department of the Horticultural Institute. In 2010, all genotypes (4 cultivars and 3 clones) were multiplied in in vitro cultures in the above-mentioned tissue culture laboratory at the Institute of Horticulture in Skierniewice. Propagated and planted plant material was used to establish the field trial in 2011 in the Experimental Orchard at Dąbrowice.
Fruits were collected at the optimum ripening time in 2016. The fresh fruits were directly frozen at −25°C, stored at −20°C, and then freeze-dried (24 h; Christ Alpha 1–4 LSC; Osterode am Harz, Germany). The homogeneous dry material was obtained by crushing the dried tissues using a closed laboratory mill (IKA A.11, Werke Staufen, Breisgau, Germany). The powders were kept in a refrigerator (−80 °C) until extract preparation.

3.3. Identification and Quantification of Polyphenols

Qualitative (LC/MS QTOF) and quantitative (UPLC-PDA-FL) analysis of polyphenols (anthocyanin, flavan-3-ol, flavonol, and phenolic acid) was performed as described previously by Oszmiański and Lachowicz [22]. All measurements were repeated three times. The results were expressed as mg per 100 grams of dry matter (dm).

3.4. Analysis of Proanthocyanidins by the Phloroglucinolysis Method

Direct phloroglucinolysis of freeze-dried samples was performed as described by Oszmiański et al. [35] using reverse-phase high-performance liquid chromatography (RP-HPLC) analysis, and phloroglucinol products were separated on a Cadenza CD C18 (75–4.6 mm, 3 μm) column (Imtakt, Kyoto, Japan) All data were obtained in triplicate. The results were expressed as mg per 100 g·dm.

3.5. Identification and Quantification of Carotenoids and Chlorophylls

For the extraction of carotenoids, a protocol similar to that described previously was applied [36]. Samples (10 μL) were eluted according to the linear gradient described by Delphino-Rius et al. [37]. The runs were monitored at 450 and 650 nm. The PDA spectra were measured over the wavelength range of 200–700 nm in steps of 2 nm. The retention times and spectra were compared to those of the authentic standards. All incubations were done in triplicate. The results were expressed as mg per kg of dm.

3.6. Identification and Quantification of Triterpenoids

Fruit sample extraction was performed as described by Farneti et al. [38]. Identification and quantification of ursolic, oleanolic, and betulinic acids was done using the ACQUITY Ultra Performance LC system with a binary solvent manager (Waters Corp., Milford, MA, USA), a UPLC BEH C18 column (1.7 μm, 2.1 mm × 150 mm, Waters Corp., Milford, MA, USA), and a Q-TOF mass spectrometer (Waters, Manchester, UK) equipped with an electrospray ionization (ESI) source, operating in negative mode. The elution solvent was methanol–acetonitrile (15:85, v/v), at a flow rate of 0.1 mL·min−1. The m/z for betulinic acid was 455.3452, for oleanolic acid 455.3496, and for ursolic acid 455.3365, and the retention times were 6.89, 7.58, and 8.19 min, respectively. The compounds were monitored at 210 nm. All data were obtained in triplicate. The results were expressed as mg per kg of dm.

3.7. Determination of Antioxidant Activity

The solvent for the analysis was prepared as described previously by Lachowicz et al. [39]. The ABTS and the FRAP assays were determined according to Re et al. [40] and Benzie and Strain [41]. Determinations by the ABTS and FRAP methods were performed using the UV-2401 PC spectrophotometer (Shimadzu, Kyoto, Japan). The antioxidant activity was expressed as mmol of Trolox per 100 g·dm.

3.8. Analysis of Sugar by the HPLC-ELSD Method

An analysis of sugar by the HPLC-ELSD method was performed according to the protocol described by Oszmiański and Lachowicz [22]. Calibration curves (R2 = 0.9999) were created for glucose, fructose, sorbitol, and sucrose. All data were obtained in triplicate. The results were expressed as mg per 100 g·dm.

3.9. Statistical Analysis

Statistical analysis, one-way ANOVA and principal component analysis (PCA) were conducted using Statistica version 12.5 (StatSoft, Kraków, Poland). Significant differences (p ≤ 0.05) between mean values were evaluated by one-way ANOVA and Duncan’s multiple range test.

4. Conclusions

The results showed an important effect of the analyzed genotypes on the composition of phytochemical compounds in the Saskatoon berry. Additionally, a positive correlation between antioxidant capacity and the content of bioactive compounds was found. The fruits of tested Saskatoon berry genotypes were found to be rich in phenolic compounds (average 5082.15 mg/100 g·dm), triterpenoids (average 78.93 mg/kg·dm), and carotenoids (average 520.10 mg/kg·dm), with high ABTS and FRAP capacity (average 22.43 and 17.5 mmol·Trolox/100 g·dm). It was also found that the fruits of the analyzed genotypes were a good source of pectins (average 1.0%), ash (average 0.63%), and sugars (average 14.36 g/100 g). Among the seven Saskatoon genotypes investigated, two new Polish clones (type S and type N) and two Canadian cultivars (“Honeywood” and “Smoky”) are characterized by the highest concentrations of phytochemical compounds and antioxidant properties. The obtained results and information will be useful for promoting the cultivation of these genotypes by commercial growers and amateurs in Poland. They will certainly attract the consumers’ interest in fruits of the Saskatoon berry due to their high contents of bioactive compounds and antioxidant capacity. In addition, they will provide a strong impetus for the food industry to develop attractive products based on the fruits of the Saskatoon berry that have potential health benefits. Valuable bioactive compounds, mainly anthocyanins, proanthocyanidins, triterpenoids, and carotenoids positive correlated with high antioxidant activity in Saskatoon berry, especially in the new clones type S and type N and cvs. “Honeywood” and “Smoky” could be applied to combat of free radicals.

Acknowledgments

This publication was supported by the Wroclaw Centre of Biotechnology, under the program The Leading National Research Centre (KNOW) for the years 2014–2018. The authors thank Elżbiecie Buckiej and Aleksandrze Borak for their technical assistance. We also thank Grzegorzowi Maryniowskiemu of the company F.H.U. BioGrim, for providing us with the fruits of the analyzed Saskatoon genotypes.

Author Contributions

Important contributions to design and in preparation of the manuscript: S.L.; Contributions to sample and analysis experiments: S.L.; Analysis of the experimental data: S.L. and Ł.S.; Critical revision for important intellectual content: S.L., J.O., and S.P.; All authors helped to prepare the paper and approved the final version.

Conflicts of Interest

The authors declare no conflict of interest.

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Sample Availability: Samples of the phytochemical composition and antioxidant capacity of seven genotypes of Sankatoon berry.
Molecules 22 00853 g001 550
Figure 1. The content of polyphenolic compounds (mg/100 g·dm) of fruits in seven Saskatoon berry genotypes analyzed in 2016.
Figure 1. The content of polyphenolic compounds (mg/100 g·dm) of fruits in seven Saskatoon berry genotypes analyzed in 2016.
Molecules 22 00853 g001
Molecules 22 00853 g002 550
Figure 2. Antioxidant activity (mmol·Trolox/100 g·dm) 1 of different Saskatoon berry genotypes analyzed in 2016. 1 Values are means ± standard deviation. n = 3.
Figure 2. Antioxidant activity (mmol·Trolox/100 g·dm) 1 of different Saskatoon berry genotypes analyzed in 2016. 1 Values are means ± standard deviation. n = 3.
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Molecules 22 00853 g003 550
Figure 3. PCA mean showing the relationship among phytochemical compounds and antioxidant activity in fruits of the Saskatoon genotypes grown in Poland. UA, ursolic acid; OA, oleanolic acid; BA, betulinic acid; FL, flavonols; ANT, anthocyanins; PA, phenolic acid; F3O, falavan-3-ols; BCN, beta-carotene; CH, chlorophylls; TS, total sugar; TCC, total carotenoids compounds; TTC, total triterpenoids compounds; PP, polymeric procyanidins.
Figure 3. PCA mean showing the relationship among phytochemical compounds and antioxidant activity in fruits of the Saskatoon genotypes grown in Poland. UA, ursolic acid; OA, oleanolic acid; BA, betulinic acid; FL, flavonols; ANT, anthocyanins; PA, phenolic acid; F3O, falavan-3-ols; BCN, beta-carotene; CH, chlorophylls; TS, total sugar; TCC, total carotenoids compounds; TTC, total triterpenoids compounds; PP, polymeric procyanidins.
Molecules 22 00853 g003
Table
Table 1. Chemical composition of fruits in different Saskatoon berry genotypes analyzed in 2016.
Table 1. Chemical composition of fruits in different Saskatoon berry genotypes analyzed in 2016.
Chemical CompoundsHoneywoodMartinPembinaSmokyClone No. 5/6Clone Type NClone Type S
Dry substance (g/100 g) 122.12 ± 0.20c 220.8 ± 0.19cde29.59 ± 0.27a22.44 ± 0.20c19.11 ± 0.17def26.73 ± 0.24b21.46 ± 0.19cd
Total acidity (g/100 g) 10.41 ± 0.00ab0.57 ± 0.01ab0.45 ± 0.00ab0.44 ± 0.00ab0.64 ± 0.01a0.43 ± 0.00ab0.37 ± 0.00ab
pH3.78 ± 0.03ab3.71 ± 0.03ab3.02 ± 0.03ab3.95 ± 0.04ab3.99 ± 0.04a3.65 ± 0.03ab3.81±0.03ab
Pectins (%) 10.95 ± 0.01a0.73 ± 0.01a0.67 ± 0.01a0.71 ± 0.01a0.93 ± 0.01a1.20 ± 0.01a1.33 ± 0.01a
Ash (%) 11.11 ± 0.01a0.84 ± 0.01a0.71 ± 0.01a0.91 ± 0.01a0.61 ± 0.01a1.03 ± 0.01a1.14 ± 0.01a
Fructose (g/100g·dm) 16.83 ± 0.06ab4.94 ± 0.04bc5.08 ± 0.05abc4.94 ± 0.04bc3.14 ± 0.03d6.07 ± 0.05ab7.29 ± 0.07a
Sorbitol (g/100 g·dm) 12.25 ± 0.02a2.41 ± 0.02a1.84 ± 0.02a3.37 ± 0.03a1.80 ± 0.02a2.19 ± 0.02a2.49 ± 0.02a
Glucose (g/100 g·dm) 17.38 ± 0.07ab2.50 ± 0.02c6.18 ± 0.06b7.16 ± 0.06ab3.35 ± 0.03c8.76 ± 0.08a9.30 ± 0.08a
Sucrose (g/100 g·dm) 10.48 ± 0.00a0.57 ± 0.01a0.59 ± 0.01a0.52 ± 0.00a0.72 ± 0.01a0.66 ± 0.01a0.61 ± 0.01a
Σ sugar16.95 ± 3.40b10.42 ± 1.79d13.69 ± 2.64c15.99 ± 2.79b9.02 ± 1.23d17.68 ± 3.68ab19.69 ± 4.05a
1 Values are means ± standard deviation, n = 3; 2 a–f Means ± SD followed by different letters within the same line represent significant differences (p < 0.05).
Table
Table 2. The content of polyphenolic compounds (mg/100 g·dm) 1 of fruits in Saskatoon berry genotypes analyzed in 2016.
Table 2. The content of polyphenolic compounds (mg/100 g·dm) 1 of fruits in Saskatoon berry genotypes analyzed in 2016.
Rt (min)MS [H − M]−/[H − M]+MS/MS Fragments (m/z)HoneywoodMartinPembinaSmokyClone No. 5/6Clone Type NClone Type S
Anthocyanins
Cyanidin-3-O-galactoside 35.79449.1685+287.25551148.97 ± 4.02b639.73 ± 2.24e458.26 ± 1.60f1107.16 ± 3.88c461.06 ± 1.61fg1013.49±3.55d1219.07 ± 4.26a
Cyanidin-3-O-glucoside 36.27449.1685+287.2451524.18 ± 1.83ab291.86 ± 1.02d209.07 ± 0.73f505.11 ± 1.77b210.34 ± 0.74e462.37 ± 1.62c556.16 ± 1.94a
Cyanidin-3-O-arabinoside 36.63419.1101+287.2451196.24 ± 0.69b109.26 ± 0.38e78.27 ± 0.27f189.10 ± 0.66c78.75 ± 0.28f173.10 ± 0.61cd208.22 ± 0.72a
Cyanidin-3-O-xyloside 37.75419.1722+287.257131.94 ± 0.46b73.46 ± 0.26e52.62 ± 0.18f127.14 ± 0.44c52.94 ± 0.19fg116.38 ± 0.41d139.99 ± 0.48a
Σ Anthocyanins 2001.32 ± 304.42b 21114.31 ± 363.17e798.22 ± 286.46g1928.50 ± 418.90c803.10 ± 248.58f1765.32 ± 411.75d2123.43 ± 442.23a
Phenolic acid
Protocatechuic acid 43.22153.3815109.65293.75±0.03ab2.13±0.02bc2.25±0.02bc5.37±0.05a1.69±0.02bc2.99 ± 0.03bc2.29 ± 0.02bc
Neochlorogenic acid 33.53353.236191.3855181.02±1.63b76.77±0.69d52.50±0.47e183.85±1.65a175.76±1.58c177.18 ± 1.59c182.42 ± 1.64ab
p-hydroxybenzoic acid 44.00137.428393.430152.06 ± 0.47a13.96 ± 0.13e33.43 ± 0.30c47.20 ± 0.42b6.27 ± 0.06f53.73 ± 0.48a30.38 ± 0.27d
Chlorogenic acid 34.74353.9997191.0571770.00 ± 7.04b384.55 ± 3.53f140.01 ± 1.38g655.63 ± 5.98d501.27 ± 4.61e670.00 ± 6.11c872.10 ± 7.96a
Galic acid 44.78169.3998125.069111.95 ± 0.09c8.21 ± 0.05g13.19 ± 0.10a9.31 ± 0.07e10.87 ± 0.08d9.03 ± 0.04ef12.45 ± 0.05b
Cryptochlorogenic acid 35.05353.236191.385585.42 ± 0.77b39.19 ± 0.35d45.50 ± 0.41c90.85 ± 0.82a32.98 ± 0.30e39.62 ± 0.36d87.16 ± 078b
4-caffeoylquinic acid 36.10353.1907173.451847.87 ± 0.1721.00 ± 0.0727.53 ± 0.1049.07 ± 0.1735.31 ± 0.1233.89 ± 0.1215.53 ± 0.05
Caffeic acid glucoside 47.53341.2219179.383840.09 ± 0.1517.59 ± 0.0623.05 ± 0.0841.09 ± 0.1429.57 ± 0.1028.38 ± 0.1013.00 ± 0.04
Dicaffeic acid 38.34353.9997191.09962.96 ± 0.011.30 ± 0.001.70 ± 0.013.03 ± 0.012.18 ± 0.032.10 ± 0.010.96 ± 0.00
Σ Phenolic acids 1195.12 ± 291.36b564.70 ± 148.55f339.15 ± 50.95g1085.40 ± 244.45c795.91 ± 196.59e1016.92 ± 256.41d1216.29 ± 340.16a
Flavonols
Kampferol-3-galactoside 36.03447.2142285.246944.92 ± 0.40a19.12 ± 0.18c18.62 ± 0.17c45.54 ± 0.41a19.35 ± 0.20c44.59 ± 0.40a41.04 ± 0.37b
Quercetin-3-O-arabinoglucoside 48.74595.1489301.231128.42 ± 0.1014.78 ± 0.0510.33 ± 0.0425.98 ± 0.0916.98 ± 0.0623.91 ± 0.0830.32 ± 0.10
Quercetin-3-O-rutinoside 39.15609.1181301.235239.54 ± 0.1420.56 ± 0.0714.37 ± 0.0536.14 ± 0.1323.62 ± 0.0833.26 ± 0.1242.18 ± 0.15
Quercetin-3-O-galactoside 39.36463.1486301.2321209.57 ± 0.73109.00 ± 0.3876.16 ± 0.27191.57 ± 0.67125.22 ± 0.44176.29 ± 0.62223.58 ± 0.78
Quercetin-3-O-glucoside †9.61463.1398301.228621.66 ± 0.0811.26 ± 0.047.87 ± 0.0319.80 ± 0.0712.94 ± 0.0518.22 ± 0.0623.10 ± 0.08
Quercetin-3-O-rabinobioside 49.82609.9368301.203714.78 ± 0.057.69 ± 0.035.37 ± 0.0213.51 ± 0.058.83 ± 0.0312.43 ± 0.0415.77 ± 0.05
Quercetin-3-O-arabinoside 39.89433.0698301.09728.35 ± 0.034.34 ± 0.043.03 ± 0.017.63 ± 0.034.99 ± 0.027.02 ± 0.028.91 ± 0.03
Quercetin-3-O-xyloside 310.01433.1374301.2257.16 ± 0.033.73 ± 0.012.60 ± 0.016.55 ± 0.024.28 ± 0.026.03 ± 0.027.64 ± 0.02
Quercetin-deoxyhexo-hexoside 310.29609.108301.2577.85 ± 0.034.08 ± 0.012.85 ± 0.017.18 ± 0.034.69 ± 0.016.61 ± 0.028.38 ± 0.02
Σ Flavonols 382.25 ± 206.77b194.57 ± 110.54f141.20 ± 73.51g353.89 ± 185.83c220.90 ± 128.83e328.35 ± 169.12d400.91 ± 225.45a
Flavan-3-ols
A-type procyanidin dimer 34.34575.168289.239822.81 ± 0.0831.07 ± 0.1146.41 ± 0.1644.91 ± 0.1621.46 ± 0.0825.26 ± 0.0924.85 ± 0.08
(+)-Catechin 35.48289.111 18.70 ± 0.0725.47 ± 0.0938.05 ± 0.1336.82 ± 0.1317.59 ± 0.0620.71 ± 0.0720.37 ± 0.07
B-type procyjanidyn dimer 35.55577.2162407.1907/289.07272.75 ± 0.013.75 ± 0.015.60 ± 0.025.42 ± 0.022.59 ± 0.013.05 ± 0.013.00 ± 0.01
(−)-Epicatechin 36.57289.2816 82.13 ± 0.29111.84 ± 0.39167.08 ± 0.59161.67 ± 0.5177.25 ± 0.2790.94 ± 0.3289.45 ± 0.32
B-type procyjanidyn trimer 47.22865.0563577.1276/287.370019.51 ± 0.0726.57 ± 0.0939.69 ± 0.1438.40 ± 0.1318.35 ± 0.0621.60 ± 0.0821.25 ± 0.07
B-type procyjanidyn tetramer 47.531153.077865.1967/577.1158/287.239836.15 ± 0.1349.23 ± 0.1773.5 ± 0.2671.16 ± 0.2034.00 ± 0.1240.02 ± 0.1439.37 ± 0.13
A-type procyanidin trimer 47.60863.168575.1267/289.246736.46 ± 0.1449.65 ± 0.1074.18 ± 0.2171.77 ± 0.1534.30 ± 0.1040.37 ± 0.1039.71 ± 0.14
Polymeric procyanidin 2563.72 ± 23.07a1602.79 ± 14.43e1269.23 ± 11.42f2259.02 ± 20.33d1189.76 ± 10.71g2480.65 ± 22.33b2411.73 ± 23.69c
Σ Flavan-3-ols 2782.24 ± 1658.31a1900.36 ± 922.93e1713.78 ± 583.14f2689.17 ± 1293.21d1395.30 ± 695.95g2722.60 ± 1583.00b2649.73 ± 1537.19c
Σ Polyphenolic compounds 6360.93 ± 57.25b3773.94 ± 52.96e2992.35 ± 26.93g6056.96 ± 54.51c3215.21 ± 28.94f5833.20 ± 52.50d6390.36 ± 57.51a
1 Values are means ± standard deviation, n = 3; 2 a–g Means ± SD followed by different letters within the same line represent significant differences (p < 0.05); 3 Identification confirmed by commercial standards; 4 Identification by comparison of MS data with the literature and their identification is tentative.
Table
Table 3. The fruit content of carotenoid, chlorophyll, and triterpenoid compounds (mg/kg of dm) 1 in seven Saskatoon genotypes analyzed in 2016.
Table 3. The fruit content of carotenoid, chlorophyll, and triterpenoid compounds (mg/kg of dm) 1 in seven Saskatoon genotypes analyzed in 2016.
Chemical CompoundsRt (min)MS [H − M]−/[H − M]+MS/MS Fragments (m/z)HoneywoodMartinPembinaSmokyClone No. 5/6Clone Type NClone Type S
Carotenoids
Zeaxanthin4.66601.0521 2.19 ± 0.02c 23.94 ± 0.04abc3.01 ± 0.03bc4.72 ± 0.04ab2.83 ± 0.03bc4.93 ± 0.04ab5.32 ± 0.05a
all-trans-lutein5.11568.0021551.1608/553.1281165.60 ± 1.53b153.16 ± 1.42d146.96 ± 1.36e164.70 ± 1.52b141.78 ± 1.31f162.49 ± 1.50c168.99 ± 1.56a
13-cis-lutein5.21568.0261551.1872/553.10234.65 ± 0.09c4.30 ± 0.01e4.13 ± 0.05g4.62 ± 0.02d3.98 ± 0.02f4.56 ± 0.01b4.75 ± 0.03a
β-carotene8.72537.1452445.0571344.23 ± 3.10c336.71 ± 3.03e324.52 ± 2.92g340.32 ± 3.06d330.98 ± 2.98f380.21 ± 3.42b382.51 ± 3.44a
Σ Carotenoids 516.67 ± 171.03c498.11 ± 166.55e478.62 ± 160.92g514.36 ± 167.81d479.57 ± 164.53f552.19 ± 188.22b561.57 ± 188.95a
Chlorophylls
Chlorophyll b7.30907.1181629.556039.81 ± 0.36e27.76 ± 0.25f56.12 ± 0.51a23.04 ± 0.21g54.12 ± 0.49ab46.23 ± 0.42c42.01 ± 0.38d
Pheophytin a8.30871.1362593.2734/533.127647.12 ± 0.42d52.34 ± 0.47b49.46 ± 0.45c48.16 ± 0.43cd61.03 ± 0.55a48.37 ± 0.44cd51.98 ± 0.47b
Σ Chlorophylls 86.93 ± 5.17d80.10 ± 17.38e105.58 ± 4.71b71.20 ± 17.76f115.15 ± 4.89a94.6 ± 1.51c93.99 ± 7.05c
Triterpenoids
Betulinic acid6.89455.3452− 11.62 ± 0.10a7.45 ± 0.07cde6.92 ± 0.06de9.09 ± 0.08bcd6.67 ± 0.06e9.31 ± 0.08bc10.84 ± 0.10ab
Oleanolic acid7.58455.3496− 71.03 ± 0.64c57.83 ± 0.52f56.02 ± 0.50f64.92 ± 0.58d62.94 ± 0.57de73.14 ± 0.66b75.38 ± 0.68a
Ursolic acid8.19455.3365− 6.19 ± 0.06a3.68 ± 0.03bcd3.61 ± 0.03bcd3.95 ± 0.04abcd2.60 ± 0.02cd4.71 ± 0.04abc5.09 ± 0.05ab
Σ Triterpenoids 88.84 ± 35.97b68.96 ± 30.23e66.55 ± 29.35f77.96 ± 33.82c72.21 ± 33.72d87.16 ± 38.25b91.31 ± 39.03a
1 Values are means ± standard deviation, n = 3; 2 a–g Means ± SD followed by different letters within the same line represent significant differences (p < 0.05).
Table
Table 4. Correlation between antioxidant activity and bioactive compounds.
Table 4. Correlation between antioxidant activity and bioactive compounds.
Polyphenolic CompoundsThe Ability to Reduce Free Radical (ABTS)Ferric Reducing Ability of Plasma (FRAP)
Betulinic acid0.8480.824
Oleanolic acid0.8400.827
Ursolic acid0.7130.452
Σ Triterpenoids0.8840.860
Σ Anthocyanins0.9320.915
Σ Phenolic acids0.8250.793
Σ Flavonols0.8820.857
Σ Flavan-3-ols−0.543−0.551
Polymeric procyanidin0.9570.935
Σ Phenolic compounds0.9250.900
Chlorophyll−0.573−0.563
Β-caroten0.8090.841
Zeaxanthin0.4960.599
Luteina0.9430.939
Σ Carotenoid compounds0.9050.928

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Lachowicz, S.; Oszmiański, J.; Seliga, Ł.; Pluta, S. Phytochemical Composition and Antioxidant Capacity of Seven Saskatoon Berry (Amelanchier alnifolia Nutt.) Genotypes Grown in Poland. Molecules 2017, 22, 853. https://doi.org/10.3390/molecules22050853

AMA Style

Lachowicz S, Oszmiański J, Seliga Ł, Pluta S. Phytochemical Composition and Antioxidant Capacity of Seven Saskatoon Berry (Amelanchier alnifolia Nutt.) Genotypes Grown in Poland. Molecules. 2017; 22(5):853. https://doi.org/10.3390/molecules22050853

Chicago/Turabian Style

Lachowicz, Sabina, Jan Oszmiański, Łukasz Seliga, and Stanisław Pluta. 2017. "Phytochemical Composition and Antioxidant Capacity of Seven Saskatoon Berry (Amelanchier alnifolia Nutt.) Genotypes Grown in Poland" Molecules 22, no. 5: 853. https://doi.org/10.3390/molecules22050853

APA Style

Lachowicz, S., Oszmiański, J., Seliga, Ł., & Pluta, S. (2017). Phytochemical Composition and Antioxidant Capacity of Seven Saskatoon Berry (Amelanchier alnifolia Nutt.) Genotypes Grown in Poland. Molecules, 22(5), 853. https://doi.org/10.3390/molecules22050853

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