Extract of Acanthopanax senticosus and Its Components Interacting with Sulfide, Cysteine and Glutathione Increase Their Antioxidant Potencies and Inhibit Polysulfide-Induced Cleavage of Plasmid DNA
Abstract
:1. Introduction
2. Results
2.1. Experiments Using ASRE
2.1.1. ASRE Interacting with H2S Reduces ●cPTIO
2.1.2. ASRE Interacting with Cys or GSH (But Not with GSSG, MET or Cystine) Reduces ●cPTIO
2.1.3. Effect of ASRE on Na2S4 Induced Reduction of ●cPTIO
2.1.4. Effect of ASRE on Its Own and in the Mixture with H2S, Polysulfides, Cys and GSH on pDNA Cleavage
2.2. Experiments Using ASRE Components: CA, CGA, Protocatechuic Acid (PCA), Eleutheroside B (EB) and Eleutheroside E (EE)
2.2.1. CA, CGA and PCA (But Not EB or EE) Interacting with H2S Reduce ●cPTIO
2.2.2. CA, CGA, PCA, EB or EE Interacting with Polysulfide Do Not Reduce ●cPTIO
2.2.3. CA, CGA and PCA (But Not EB or EE) in the Mixture with Cys or GSH Reduce ●cPTIO
2.2.4. Effect of the ASRE Components on pDNA Cleavage
3. Discussion
4. Materials and Methods
4.1. Chemicals and Solutions
4.2. Preparation of Aqueous Root Extract from Acanthopanax Senticosus (ASRE)
4.3. UV-VIS of ●cPTIO Radical
4.4. Plasmid DNA Cleavage
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Misak, A.; Grman, M.; Tomasova, L.; Makara, O.; Chovanec, M.; Ondrias, K. Extract of Acanthopanax senticosus and Its Components Interacting with Sulfide, Cysteine and Glutathione Increase Their Antioxidant Potencies and Inhibit Polysulfide-Induced Cleavage of Plasmid DNA. Molecules 2022, 27, 5735. https://doi.org/10.3390/molecules27175735
Misak A, Grman M, Tomasova L, Makara O, Chovanec M, Ondrias K. Extract of Acanthopanax senticosus and Its Components Interacting with Sulfide, Cysteine and Glutathione Increase Their Antioxidant Potencies and Inhibit Polysulfide-Induced Cleavage of Plasmid DNA. Molecules. 2022; 27(17):5735. https://doi.org/10.3390/molecules27175735
Chicago/Turabian StyleMisak, Anton, Marian Grman, Lenka Tomasova, Ondrej Makara, Miroslav Chovanec, and Karol Ondrias. 2022. "Extract of Acanthopanax senticosus and Its Components Interacting with Sulfide, Cysteine and Glutathione Increase Their Antioxidant Potencies and Inhibit Polysulfide-Induced Cleavage of Plasmid DNA" Molecules 27, no. 17: 5735. https://doi.org/10.3390/molecules27175735
APA StyleMisak, A., Grman, M., Tomasova, L., Makara, O., Chovanec, M., & Ondrias, K. (2022). Extract of Acanthopanax senticosus and Its Components Interacting with Sulfide, Cysteine and Glutathione Increase Their Antioxidant Potencies and Inhibit Polysulfide-Induced Cleavage of Plasmid DNA. Molecules, 27(17), 5735. https://doi.org/10.3390/molecules27175735