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Review

Biohydrogen Production: Strategies to Improve Process Efficiency through Microbial Routes

by
Kuppam Chandrasekhar
†,
Yong-Jik Lee
† and
Dong-Woo Lee
*
School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2015, 16(4), 8266-8293; https://doi.org/10.3390/ijms16048266
Submission received: 21 January 2015 / Revised: 1 April 2015 / Accepted: 3 April 2015 / Published: 14 April 2015
(This article belongs to the Special Issue Photosynthesis and Biological Hydrogen Production)
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Abstract

:
The current fossil fuel-based generation of energy has led to large-scale industrial development. However, the reliance on fossil fuels leads to the significant depletion of natural resources of buried combustible geologic deposits and to negative effects on the global climate with emissions of greenhouse gases. Accordingly, enormous efforts are directed to transition from fossil fuels to nonpolluting and renewable energy sources. One potential alternative is biohydrogen (H2), a clean energy carrier with high-energy yields; upon the combustion of H2, H2O is the only major by-product. In recent decades, the attractive and renewable characteristics of H2 led us to develop a variety of biological routes for the production of H2. Based on the mode of H2 generation, the biological routes for H2 production are categorized into four groups: photobiological fermentation, anaerobic fermentation, enzymatic and microbial electrolysis, and a combination of these processes. Thus, this review primarily focuses on the evaluation of the biological routes for the production of H2. In particular, we assess the efficiency and feasibility of these bioprocesses with respect to the factors that affect operations, and we delineate the limitations. Additionally, alternative options such as bioaugmentation, multiple process integration, and microbial electrolysis to improve process efficiency are discussed to address industrial-level applications.

Graphical Abstract

1. Introduction

The growing gap between the energy demand of the world and an insufficient energy supply has caused a steep increase in fossil fuel use. As a result, we encounter the severe constraints imposed by an alarming increase in pollution levels around the world along with the depletion of fossil fuels. Additionally, the continuous increase in the levels of greenhouse gases (GHGs) released from the combustion of fossil fuels aggravates the problems of global warming. Currently, the CO2 concentration exceeds 350 parts per million (ppm) by volume, and the increase in concentration potentially increases the greenhouse effect, which results in increasing global temperatures [1,2]. In recent decades, the organic carbon released by human activities is equivalent to that which was accumulated over millions of years. The limited availability of global fossil fuel reserves and concerns about global climate change from GHG emissions prompted notable interest in the investigation and development of eco-friendly, renewable energy alternatives to fulfill the growing energy demands [3]. Therefore, in the current global energy scenario, the diversification of energy and fuel options is an essential requirement [4]. To diversify, bio-based energy is a sustainable and promising alternative to fossil fuel-based energy; this alternative energy can defend against a crisis in the energy supply and can protect the world from the approaching environmental calamity. Recently, global attention focused on hydrogen (H2) gas as one of the most promising, eco-friendly, and renewable energy sources. H2 is a potentially versatile energy currency that could alter the use of liquid fossil fuels because the fuel has a high-energy yield per unit mass of 122 kJ/g, which is 2.75-fold higher than that of hydrocarbon fuels [5,6]. Additionally, the combustion of H2 with O2 produces water (H2O) as the only by-product, an obviously favorable outcome for a reduction in GHG emissions. In particular, H2 is the pre-eminent choice for an energy carrier because it is more similar to electricity than fossil fuels in the framework of energy systems [7].
Currently, molecular H2 is primarily produced from the use of fossil fuels through steam reforming of natural gas or methane (CH4). The worldwide production of H2 currently exceeds 1 billion m3/day of which 48% is produced from natural gas, 30% from oil, 18% from coal, and the remaining 4% is produced from H2O-splitting electrolysis [2,8]. In combination with steam reforming, the production of pure H2 is also achieved with an H2O-gas shift reaction, which is one of the important industrial reactions used specifically for ammonia production. The other thermochemical methods available for the production of H2 include thermal decomposition, autothermal reforming, catalytic oxidation, pyrolysis, and steam gasification [2,9,10]. However, the production of H2 based on fossil fuel resources increases the emissions of GHGs. Alternatively, the production of H2 from biomass through biological pathways is an emerging technology because it is sustainable and eco-friendly. Indeed, a scientometric analysis that used the SCI-expanded (since 1994), science technology (CPCI-S, since 1994), and social science (CPCI-SS, since 1994) databases in the ISI Web of Knowledge (Thomson Reuters) found that 2204 research articles were published on H2, with a significant number of citations (46,723) and average citations per item (21.02), and a high H-index (92). As shown in Figure 1, the literature linked to H2 research increased sharply after 2003 and reached the maximum number of records of 230 in 2008 (total citations: 2883), which was followed by a sudden increase in 2012 (records, 335; citations, 8107). The average citations per year also increased year by year, which clearly indicated that the rapid and promising research continues to make the process of H2 production technologically viable.
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Figure 1. Scientometric analysis of the research on H2 production. Published items (A) and citations (B) in each year.
Figure 1. Scientometric analysis of the research on H2 production. Published items (A) and citations (B) in each year.
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Different organisms yield H2 under specific conditions, including microalgae that use light energy to split water molecules to produce H2, and cyanobacteria that typically consume carbohydrates to store energy from photosynthesis to produce H2 from water molecules [11,12]. Although there are striking advantages, the low production rates, low substrate conversion efficiencies, and production and accumulation of acid-rich intermediate metabolites from the acidogenic process are practical hindrances that must be overcome for the successful biological production of H2. To overcome these limitations, many research projects on the biological production of H2 are in progress, and numerous novel approaches are being studied to address some of the existing problems and to overcome these problems by increasing the efficiency of the process. To reach these goals, a number of advanced well-described technologies for high yields of molar H2 use metabolic engineering to provide metabolic energy to exceed thermodynamic limitations, to reroute metabolic pathways to increase substrate utilization by the expression of heterologous proteins, and to improve the electron flux for H+ reduction, among others [11,12]. In this review, we evaluate the biological pathways for the production of H2 with respect to the factors that affect operations and potentially limit the production of H2, and assess the efficiency and practical applicability of these technologies. Additionally, alternative options such as bioaugmentation, multiple process integration, and microbial electrolysis to improve process efficiency are discussed.

2. Biohydrogen

2.1. Diversity of Microorganisms as H2 Producing Biocatalysts

In nature, a variety of organisms including the archaea, anaerobic and facultative aerobic bacteria, cyanobacteria, and lower eukaryotes (i.e., green algae and protists) produce H2 [12,13], which may function singly or as a consortium of similar types or mixed cultures (Figure 2). The major H2 producing biocatalysts are typical heterotrophs in the fermentation process. Some dark fermentative bacteria do not require solar energy as an energy source and tolerate O2 deficient conditions; these microorganisms are obligate anaerobes, which are further classified based on their sensitivity to O2 and their growth temperature (Figure 2).
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Figure 2. Schematic representation of the diversity of H2 producing biocatalysts.
Figure 2. Schematic representation of the diversity of H2 producing biocatalysts.
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Practically, the culture and maintenance of facultative anaerobes are more feasible than for obligate anaerobes. Based on their growth temperatures, these microorganisms are further classified into mesophiles and thermophiles. Although the thermophiles are cultivated at elevated temperatures with highly intensive energy requirements [14], their H2 production can be closer to the theoretical yield than with mesophiles by overwhelming the thermodynamic barrier. Some photofermentative bacteria require light energy to produce H2 in anoxygenic conditions. In the absence of O2, these photoautotrophs, which include cyanobacteria and green algae, produce H2 through biophotolysis using their specific metabolic routes advantageously under defined conditions.
Briefly, based on the systems that evolve H2, a large number of different natural biological processes are categorized into four primary groups: (1) water-splitting photosynthesis; (2) photofermentation; (3) dark fermentation; and (4) microbial electrolysis processing (Figure 2 and Figure 3). For energy efficiency and practicality, each process has advantages and disadvantages when compared with the other methods. Accordingly, the selection of an appropriate biocatalyst and/or inoculum is an important choice, one that is directly correlated with H2 production. Naturally, H2 is produced either by a single microbial species or by a mixed consortium of species of which some are involved in the production of H2, and the rest consume the H2 for their energy requirements. Initial research on H2 was typically confined to the use of pure cultures as a biocatalyst with a defined substrate as the carbon source. However, when wastewater was used as the substrate, a mixed microbial population was favorable and practical for application to the scaled-up production of H2 [15]. Additionally, mixed cultures are typically preferred because of operational ease, stability, diversity of biochemical functions, and the possibility to use a wide range of substrates [16,17,18]. Therefore, for the practical microbial production of H2 in the near future, the proper choice of a H2-evolving biosystem together with a deep understanding of the biochemical and biophysical characteristics of the system is a key requirement.
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Figure 3. Schematic representation of the primary biological routes integrated with various secondary processes for effective H2 production.
Figure 3. Schematic representation of the primary biological routes integrated with various secondary processes for effective H2 production.
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2.2. Water-Splitting Photosynthesis (Biophotolysis)

Currently, the most desirable and attractive H2 production process is water-splitting photosynthesis, or biophotolysis. The oxygenic photosynthetic microorganisms such as green microalgae (e.g., Scenedesmus obliquus, Chlamydomonas reinhardtii, Chlorella, and Scenedesmus, among others) and cyanobacteria (e.g., Anabaena variabilis, Nostoc punctiforme, and Synechocystis sp., among others) use this process that requires only water and sunlight. A (FeFe)-hydrogenase in green algae drives the evolution of H2, whereas nitrogenase is responsible for this process in heterocystous cyanobacteria. The biophotolysis is further divided into direct and indirect processes (Figure 3). As shown in Figure 4A, in direct biophotolysis, the electrons derived from the light energy-mediated water splitting are transferred through photosystem II (PS II) and photosystem I (PS I) to ferredoxin (Fd) as an electron carrier, and subsequently, the reduced Fd reduces a hydrogenase enzyme that is responsible for H2 production [19]: 2H+ + 2Fd(re) ↔ H2 + 2Fd(ox). In the case of indirect biophotolysis, photosynthesis converts light energy to chemical energy in the form of a carbohydrate, which is reused to produce H2, and at present, these H2 producing systems are being intensively investigated using green algae and heterocystous cyanobacteria [12,20]. Because the production of H2 by cyanobacteria occurs in the heterocyst, and the oxygenic photosynthesis is microscopic indirect biophotolysis, which is concomitant with CO2 fixation in the vegetative cell, the highly O2-sensitive nitrogenase is protected, resulting in the production of H2: N2 + 8e− + 8H+ + 16ATP → 2NH3 + H2 + 16ADP + 16Pi. However, H2 production by (FeFe)-hydrogenase and oxygenic photosynthesis cannot occur simultaneously in green algae. Thus, to obtain sustainable H2 production, elemental sulfur (S°) deficiency, which causes a severe (≈90%) reduction in photosynthesis, occurred with cells grown on acetate, resulting in a drastic decrease in the oxygen production rate coupled with the improved respiration caused by the existence of residual acetate. In this condition, the cells grow in anaerobic conditions to produce H2 by using some of the electrons from the residual water-splitting mechanism (direct biophotolysis) and the reserved carbon (indirect biophotolysis) [21,22].
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Figure 4. Schematic illustration of H2 evolution through (A) direct/indirect biophotolysis and (B) dark fermentation: (A) PS II, photosystem II; PQ, plastoquinone; PQH2, plastoquinol; cyt b6f, cytochrome b6f complex; PC, plastocyanin; PS I, photosystem I; Fd, ferredoxin; and FNR, ferredoxin-NADP+ reductase. Approximately half of the evolved H2 is from water splitting, and the rest of the H2 is produced with e− made from the fixed carbon by the activity of the PS I; (B) Q, quinone; QH2, quinol; cyt bc1, cytochrome bc1 complex; and cyt aa3, the cytochrome aa3 oxidase.
Figure 4. Schematic illustration of H2 evolution through (A) direct/indirect biophotolysis and (B) dark fermentation: (A) PS II, photosystem II; PQ, plastoquinone; PQH2, plastoquinol; cyt b6f, cytochrome b6f complex; PC, plastocyanin; PS I, photosystem I; Fd, ferredoxin; and FNR, ferredoxin-NADP+ reductase. Approximately half of the evolved H2 is from water splitting, and the rest of the H2 is produced with e− made from the fixed carbon by the activity of the PS I; (B) Q, quinone; QH2, quinol; cyt bc1, cytochrome bc1 complex; and cyt aa3, the cytochrome aa3 oxidase.
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Recent improvements also include the first direct demonstration of an indirect biophotolysis process that used the nitrogenase enzyme in which the nonheterocystous cyanobacterium Plectonema boryanum was recycled multiple times through an aerobic, nitrogen-limited stage, which led to glycogen accumulation, and a second anaerobic, H2-producing stage [23]. Additionally, sustained H2 production by a single-celled, nonheterocystous cyanobacterium Cyanothece occurred with growth in medium supplemented with glycerol for respiratory protection [24] or by replacement of the photosynthetically evolved O2 with Argon (Ar) gas [2,25]. Nevertheless, before practical applications, biophotolysis-mediated H2 production systems require considerable efforts in protein engineering research to develop O2-tolerant hydrogenases in green algae or to replace hydrogenase with nitrogenase in cyanobacteria [11]. Recently, other potential strategies to improve H2 production were proposed and investigated, including a decrease in the antenna size [26], downregulation or mutation of the PS II proteins [2,27], changes in operational conditions [28], and heterologous expression of hydrogenase and Fd [2,29].

2.3. Anoxygenic Photofermentation

Photofermentation also involves the conversion of light energy to biomass with the production of H2 and carbon dioxide (CO2); often, the relation is nearly stoichiometric. For the process of photofermentation, purple nonsulfur (PNS) photosynthetic bacteria, including Rhodobacter species, are used to convert organic acids such as acetate, lactate, and butyrate to H2 and CO2 in anaerobic and anoxic conditions. Moreover, these bacteria capture solar energy to transform organic acids into H2 using nitrogenases in the absence of ammonium (NH4) ions [2,30,31]. In particular, O2-sensitive nitrogenase is not a problem for this process because the purple bacteria used in the process have nonoxygenic photosynthesis [30]. However, these nitrogenases also possess several defects that affect the production of H2, including low catalytic activity, suppression of their expression by NH4, and lower photochemical efficacy [32,33]. In theory, the photofermentation process can completely convert organic compounds into H2, even reaching a comparatively high H2 partial pressure; simultaneously, H2 production is driven by nitrogenase, and ATP is formed with the capture of solar energy through a photosynthetic mechanism in these bacteria (e.g., Allochromatium vinosum, Thiocapsa roseopersicina, R. sphaeroides, Chlorobium vibrioforme, Desulfuromonas acetoxidans, and Chloroflexus aurantiacus). Recently, a comparatively high-yield conversion efficacy of sugars to H2 was achieved [34], and the completely stoichiometric transformation of glycerol to H2 was attained [35,36]. These are light-dependent processes in which captured light energy drives the electron stream through the photosynthetic system, leading to a proton gradient. This proton gradient is further used to fulfill both requirements for nitrogenase activity: ATP, produced with ATP synthase, and high-energy electrons, generated through inverse electron flow.

2.4. Dark Fermentation

The dark fermentative process also produces H2 (Figure 4B). To date, many of the studies on the biological production of H2 through the dark fermentation process were performed using facultative (e.g., Enterobacter aerogenes, E. cloacae, Escherichia coli, and Citrobacter intermedius, among others) and obligate anaerobic bacteria (e.g., Clostridium beijerinckii, C. paraputrificum, and Ruminococcus albus, among others) [37,38,39]. The process of dark fermentation occurs at a higher rate than the processes of photofermentation and photolysis. However, the low yield of H2 on substrates, because of the formation of various by-products, is the primary disadvantage. The fermentation process helps to generate energy-rich reducing compounds (i.e., NAD(P)H and FADH) from metabolic pathways, which are then sequentially reoxidized by respiratory chains with a terminal electron acceptor (TEA), resulting in the formation of energy-rich molecules (ATP). In aerobic respiration, O2 is a TEA that helps to generate ATP with concurrent regeneration of the reducing powers. By contrast, anaerobic respiration uses a variety of organic and inorganic compounds (e.g., NO3− and SO42−, among others) as TEAs with their concurrent reduction and regeneration of reducing powers. Glycolysis is the key metabolic pathway in which a substrate can be transformed into pyruvate, a central metabolic intermediate. Under anaerobic conditions, the pyruvate enters into the acidogenic pathway coupled with H2 production, which results in the formation of volatile fatty acids (VFAs) (i.e., acetic acid, propionic acid, butyric acid, and malic acid, among others) (Equations (1)–(5)).
C6H12O6 + 2H2O → 2CH3COOH + 2CO2 + 4H2 (acetic acid pathway)
C6H12O6 + 2H2 → 2CH3CH2COOH + 2H2O (propionic acid pathway)
C6H12O6 → CH3CH2CH2COOH + 2CO2 + 2H2 (butyric acid pathway)
C6H12O6 + 2H2 → COOHCH2CH2OCOOH + CO2 (malic acid pathway)
C6H12O6 → CH3CH2OH + CO2 (ethanol pathway)
As stated above, both obligate and facultative bacteria produce H2using a wide variety of organic substrates [11]. Facultative anaerobic microorganisms transform pyruvate to acetyl-CoA and formate with the catalysis of pyruvate formate-lyase, and then produce H2 with formate hydrogen lyase [40]. However, obligate anaerobic microorganisms convert pyruvate to acetyl-CoA and CO2 with pyruvate ferredoxin oxidoreductase. This process of oxidation requires the reduction of Fd [41]. During fermentation, the H+ reducing mechanism facilitates the production of H2 as a by-product. The interconversion of metabolic intermediates occurs during substrate consumption in anaerobic fermentation, which increases the availability of reducing equivalents in bacterial cells [42,43]. The H+ released from NADH/FADH with NADH dehydrogenase is reduced to H2 by hydrogenase with the reduced Fd, whereas the membrane-bound protein complexes (i.e., NADH dehydrogenase and cytochrome bc1) and the mobile electron carriers (quinone (Q) and cytochrome c) facilitate the electron transfer through the Q pool. The continuous redox interconversion between Q (QH2) and H+ conveys electrons to the cytochrome bc1 complex (cyt bc1) and to the cytochrome aa3 (cyt aa3). Consequently, the cyt aa3 reduces the Fe-S containing Fd, which donates an electron to the hydrogenase responsible for H2 production [2,40,43].

2.5. Microbial Electrolysis Cells (Electrofermentation)

Microbial electrolysis cells (MECs), a new technique to produce H2 from a wide variety of substrates, have been rapidly developed in the last few years. These are fundamentally adapted microbial fuel cells (MFCs), which have been investigated for decades. Using the MECs as an alternative electrically driven H2 production process results in the conversion of a wide range of organic substrates into H2 under applied external potential. The MEC technology is also called electrofermentation or biocatalyzed electrolysis cells [44,45]. The MEC technology resembles an MFC in which the primary difference is the necessity of a small input of external voltage. Based on thermodynamics, a potential higher than 0.110 V, in addition to that generated by a microorganism (−0.300 V), will produce H2 [44]. The normal redox potential for the reduction of H+ to H2 is −0.414 V; therefore, the potential requirement is very low when compared with the theoretically required voltage of 1.230 V for the electrolysis of H2O [45].
In practice, however, a comparatively higher potential than this value is required because of the over potentials created by physical, chemical, and microbial factors [2,46]. The MECs are capable of more than 90% efficiency in the production of H2 [44]. However, the performance of MECs is determined by the type of microorganism, electrode materials, type of the membrane used, applied potential range, composition and concentration of the substrate, and design of the MEC. Initially, the MECs were used in a two-chamber configuration, which was shifted later to a single chamber configuration because of the ease of the process, a significant reduction in internal resistance, a decrease in pH in the anode chamber caused by the production and accumulation of H+, and an increase in pH in the cathode chamber caused by the use of H+, and the provision of a membrane is avoided [46]. Indeed, the exclusion of a membrane reduced both the pH and the losses of energy and ohmic energy in a single chamber MEC operation [47], which was the primary problem with a two-chamber MEC configuration [45].

3. Effects of Operational Factors for H2 Production

3.1. Temperature

One of the key factors during the fermentation process is the operating temperature because it can alter not only the microbial use of the substrate and the specific growth rate but also the H2 production and the metabolic product formation. Several studies reported on dark fermentative H2 production at different temperatures: ambient (15–27 °C), mesophilic (30–45 °C), thermophilic (50–60 °C), and extremely thermophilic (>60 °C) [48,49]. The optimal growth temperatures for mesophilic bacteria (e.g., C. butyricum and E. cloacae) and hyperthermophiles (e.g., Pyrococcus furiosus and Caldicellulosiruptor bescii) ranged from 30 to 45 °C and from 50 to 80 °C, respectively. However, when mixed cultures were used, the optimal temperature for H2 production changed from the optimal growth temperature for each strain [17,48,50]. Most of the H2 production studies at the laboratory scale were performed using mesophilic microorganisms because of the ease of operation and the maximum specific growth rates [51]. Tang et al. [50] investigated the effects of temperature on H2 production with mixed cultures and demonstrated that the maximal yield of H2 (319 mL of H2/g substrate, measured in the form of COD) was obtained by increasing the temperature from 35 to 45 °C, whereas the yield decreased to 182 mL of H2/g substrate as the temperature increased from 45 to 55 °C. Lin et al. [52] also performed studies to optimize the temperature for H2 production with a mixed microbial population using a chemostat-type H2 bioreactor, and the highest H2 production was achieved at 45 °C.
Recently, much attention focused on thermophiles as attractive alternatives for the production of H2 [53]. Notably, the production of H2 benefits from some general advantages that are gained by performing processes at elevated temperatures, such as a lower viscosity, better mixing, less risk of contamination, higher reaction rates, and no need for cooling of the bioreactor. Additionally, the yields of H2 using thermophiles could reach the maximum theoretical value of 4 mol H2/mol glucose, which is much higher than examples with mesophiles that reached the maximum yield of less than 2 mol H2/mol glucose [53,54]. Indeed, operation at high temperatures is thermodynamically favorable for increased H2 production because of an increase in the entropy of the system, which makes the system more energetic and avoids contamination of the H2-utilizing enzymes and microorganisms [50]. Overall, one of the key factors for H2 production is clearly operational temperature, which typically depends on the type of the H2-producing microorganism and on the type of substrate used.

3.2. pH

The pH of the system (redox microenvironment) is an essential index for the microbial population and is an integral expression of the redox conditions for any anaerobic process [55,56]. Thus, pH has a key role in the regulation of metabolic pathways and in the production of H2 because the fermentative metabolites derived from pyruvate determine the yield of H2 [57]. Accordingly, changes in external pH values also affect several physiological parameters in cells, such as the internal pH, proton motive force, and membrane potential. As the rate-limiting factor, these results clearly indicate that the pH-dependent activity of the microorganisms directly affects the pH-dependent fermentations associated with H2 production [58]. For example, in an acidic microenvironment (low pH), pyruvate is converted to VFAs, concomitant with H2 production, whereas a neutral pH leads to CH4 production by methanogenic microorganisms [43]. Indeed, H2 producing microorganisms function well below a pH of 6, whereas the optimum pH for methanogenic microorganisms is between 6.0 and 7.5. The production of H2 was high when the pH was maintained at approximately 6.0 [59], but an extremely acidic microenvironment (pH < 4.5) was detrimental to the ability of the microbial community to produce H2 [58,60]. In an alkaline microenvironment, fermentative pathways are prone to solventogenesis [18]. Additionally, the H+ that shuttles among metabolic intermediates causes the formation of reduced compounds (e.g., aldehydes, alcohols, and reducing sugars) and altered membrane potentials, which results in decreased cellular growth rates [61,62]. The accumulation of VFAs during the fermentation process leads to a decrease in the system pH, which decreases the system buffering capacity and, ultimately, inhibits H2 production [18,60,63]. The pH range of 5.5–6.0 might be beneficial for the production of H2 with dark fermentation and for the prevention of methanogenesis and solventogenesis [18].

3.3. Nutrients

In the presence of a carbon (C) source, the supplementation with nutrients for bacterial growth is also critical to increase H2 production. The study of Lin and Lay demonstrated that at a carbon/nitrogen (C/N) ratio of 47, the productivity and the production rate of H2 reached 4.8 mol H2/mol sucrose and 270 mmol H2/L per day, respectively [63]. In a mixed culture, the maximum H2 production potential of 291.4 mL (corresponding to 3.25 mmol), the maximum H2 yield of 298.8 mL/g of glucose (3.33 mmol H2/g of glucose), and the maximum average H2 production rate of 8.5 mL/h were obtained at the ammonia concentration of 0.1 g of N/L [64]. However, excess amounts of nitrogen affected the intracellular pH of the microorganism used for the production of H2 and also inhibited the activity of nitrogenase [65,66]. Additionally, high concentrations of N induced ammonification, which was not favorable for H2 production [67]. An optimum C/N ratio influenced the microbial growth rate and H2 yield in mixed or pure cultures; however, it also adds to the overall production costs [68]. Thus, the research is in progress to find alternative sources of N in the substrate. For example, the wastes of the corn starch manufacturing process (corn-steep liquor) offer a promising alternative supplementation for peptone [69]. The optimal concentrations of phosphate (P) are also essential to increase the yield of H2 in which P is an important inorganic nutrient for the fermentative production of H2 [63]. Phosphorus, in the form of adenosine triphosphate (ATP), has a major role in energy generation in the bacterial cell and is also involved in the system that regulates the buffering capacity as an alternative to carbonate [42]. Nevertheless, a high concentration of P stimulates excessive production of VFAs, which leads to significant decrements in the yield of H2 with the diversion of cellular reductants away from the production of H2.
For any fermentative process, supplementation with suitable metal ions is necessary to activate many of the enzymes and coenzymes that are related to microbial metabolism and cellular transport processes and that are also essential for cell growth. Each of the metal ions has a precise function in the cell during metabolism, and a change in their availability may change that function [70]. Hydrogenase, a crucial enzyme in the H2 production process, has a bimetallic Fe–Fe center surrounded by Fe-S protein clusters [71]. The iron (Fe) also acts as an active site for the Fd, which transports electrons to the hydrogenase. Consequently, several researchers investigated the consequences of Fe supplementation for H2 production. Lee et al. [72] investigated the effect of Fe concentration on H2 production and observed that high concentrations had a positive effect on the system because the Fe is an essential component of the Fd and hydrogenase [73,74,75]. The optimal concentration of Fe was approximately 25 to 100 mg/L, and higher concentrations than those led to toxicity [73,76]. In another investigation, Lin and Shei [70] examined the influence of different trace elements, specifically Fe, Ni, Mg, Mn, Na, Zn, K, I, Co, Cu, Mo, and Ca, for H2 production using C. pasteurianum and found that the appropriate concentrations of Mg, Na, Zn, and Fe were essential to achieve the highest yields of H2 [70,73,74,77].

3.4. Hydraulic Retention Time

The hydraulic retention time (HRT) is also an important factor in the selection of microorganisms because microorganisms are required with growth rates that can withstand the mechanical dilution caused by continuous volumetric circulation. An extended fermentation time is unfavorable for H2 production because of the metabolic shift from acidogenesis to methanogenesis. Preferably, a shorter HRT would restrict the growth rate of methanogenic microorganisms [78]. For satisfactory H2 yields, the optimum HRTs were between 8 and 14 h for a wide variety of substrates [78,79,80]. By maintaining short HRTs (2–10 h), the methanogenesis was effectively suppressed [81]. However, for the optimum H2 yield, the HRT is influenced by several factors, including the type and composition of the substrate, the type of microorganism, the organic loading rate, and the system redox condition, among others. Unlike the dark fermentation process, a short HRT reduces the substrate use efficiency and therefore decreases the process efficiency during photofermentation [82].

3.5. Partial Pressure of H2

The biological pathways of H2 production are highly sensitive to the partial pressure of H2 (HPP), which is a key rate-limiting factor, particularly during the process of dark fermentation because hydrogenase activity (transfer of an electron from an intracellular electron carrier to H+) is likely to decrease because of feedback inhibition [16]. When the level of H2 dissolved in the fermentation medium increased, the reduction of oxidized Fd occurred more favorably than the oxidation of reduced Fd, which caused the hydrogenase to be reversibly oxidized and the Fd to be reduced, resulting in a decrease in H2 production because of the oxidation of the dissolved H2 [18]. The metabolic pathways shift from acidogenesis to solventogenesis to form reduced products, such as lactate, ethanol, acetone, butanol, and alanine, which leads to a decrease in H2 yield under higher Hpp conditions (greater than 60 Pa) because of thermodynamically unfavorable conditions [83]. The sparging of inert gases (nitrogen and argon, among others) into the reactor headspace in combination with gas stripping to maintain low Hpp successfully increased the H2 yield by 68% [18,84]. Therefore, operating H2 bioreactors at a low Hpp leads to high yields of H2 [16,18,85].

4. Economic Feasibility and Technical Challenges

During the last two decades, several efforts to make the H2 production process economically more feasible were attempted [46]. However, some key technical challenges remain, and if these challenges are overcome, the overall H2 production efficiency will increase through the biological pathways described below (Table 1).
Table
Table 1. Biological pathways for H2 production and the technical limitations.
Table 1. Biological pathways for H2 production and the technical limitations.
Type of BioprocessTechnical Challenges
Dark fermentation
  • low substrate conversion efficiency
  • low H2 yield
  • thermodynamic limitations
  • mixture of H2 and CO2 gases as products, which require separation
Photofermentation
  • requirement of an external light source
  • the process is limited by day and night cycles, with sunlight as the light source
  • low H2 yield caused by extremely low light conversion efficiency
Direct biophotolysis
  • O2 generation caused by the activity of PS II
  • requirement for customized photobioreactors
  • low H2 yield caused by extremely low light conversion efficiency
Indirect biophotolysis
  • lower H2 yield caused by hydrogenase(s)
  • requirement of an external light source
  • total light conversion efficiency was very low
These challenges may be overcome with the efficient design of H2 producing bioreactors, process modifications, selection of appropriate feedstocks, and with the selection of suitable and efficient microbial strains. In the metabolic pathways that produce H2, the intermediate metabolites produced by the biocatalyst compete for the identical reductants as the H2, and this redirection of the reductants toward soluble end metabolites reduces the H2 yields. Hence, several researchers are attempting to reroute the metabolic pathways to reduce the production of the low-end metabolites. To overwhelm the stoichiometric limitation (4 mol H2/mol glucose) of the dark fermentation process, a robust biocatalyst must be found that can be metabolically engineered. Thus, with a successful bioreactor design and the determination of the ideal process parameters, the yield of H2 can be increased. However, low molar conversion rates affect the economics of the process, and therefore, research is underway to increase the H2 yield above the 4 mol H2/mol glucose limitation. Recently, several researchers focused on the development of suitable hybrid processes, such as the two-stage integration of the dark fermentation process, followed by the photofermentative process to produce H2 [18]. With this approach, the VFAs that are produced in the dark fermentation (first stage) are used as the substrate in the photofermentation (second stage). This approach might use efficiency to increase the theoretical limit of H2 yield to 12 mol H2/mol glucose [14]. Similarly, hybrid processes that include subsequent methane production or electrofermentation are also being considered to increase the energy recovery of the process. Although there is an abundance of research in the past and at present, these specific areas must be investigated to further enhance the production of H2 via the biological pathways [37]. Additionally, the integration of the H2 production process with a conventional wastewater treatment process has several advantages, such as waste remediation with simultaneous generation of clean energy. In the future, carbon-rich organic wastes may be targeted as suitable feedstocks for H2 production because of their natural abundance. The use of cheaper raw material substrates would increase the H2 yield from the biological processes, which would help significantly to make the process more economically viable and cost effective.

5. Strategies to Enhance the Efficiency of the Process

The major deterrents to the conventional biological H2 production from any of the processes described above are the low substrate conversion efficiency and the accumulation of VFAs. Because of these deterrents, the overall yield of H2 is far too low for the process to be economically feasible and commercially applicable [38,79]. In particular, although the theoretical H2 production could reach 12 mol of H2/mol glucose, the dark fermentative H2 production is metabolically limited to 4 mol H2/mol glucose, which is a major technical hurdle for practical applications [86]. Additionally, after dark fermentation, significant amounts of residual organic substances such as VFAs or solvent remain in the effluent. Thus, additional treatments are necessary before disposal into the environment. The reuse of the residual carbon fraction of the fermentative effluents for further energy generation together with proper environmental treatment would be wise considering the environmental and economic factors [87]. Moreover, the design and fabrication of photobioreactors that use the internal light supply efficiently remains a challenge in photofermentation [88,89].

5.1. Integration of Approaches

Recently, many integrated approaches were proposed to overcome the limitations of several processes to increase the production of H2 in dark fermentation. The use of the residual acid-rich organic substances from the fermentation effluents as carbon-rich substrates for further energy recovery is a viable and novel idea, particularly when in the form of an integrated two-stage energy producing process (Table 2 and Figure 3). Numerous secondary processes, including methanogenesis for methane, acidogenic fermentation for H2, photobiological processes for H2 [90,91,92], MECs for H2 [44], anoxygenic nutrient-limiting processes for bioplastics, cultivation of heterotrophic algae for lipids, and MFCs for bioelectricity generation, were integrated with the primary dark fermentative process of H2 production. With these integrated approaches, the primary process uses these further substrates for additional energy production, and therefore, the entire process is more economically viable and practically applicable than without the integration.
Table
Table 2. A list of the processes integrated with the production of H2 from dark fermentation (DF, dark fermentation; PF, photofermentation; MEC, microbial electrolysis cell; BEH, bio-electrohydrolysis).
Table 2. A list of the processes integrated with the production of H2 from dark fermentation (DF, dark fermentation; PF, photofermentation; MEC, microbial electrolysis cell; BEH, bio-electrohydrolysis).
SubstrateFirst StageSecond StageReference
Process TypeYieldProcess TypeYield
CornstalksHydrogen (DF)58.0 mL/gMethane (DF)200.9 mL/g[93]
Rice strawHydrogen (DF)20 mL/gMethane (DF)260 mL/g[94]
Water hyacinthHydrogen (DF)38.2 mmol H2/L/dayMethane (DF)29 mmol CH4/L/d[95]
Water hyacinthHydrogen (DF)51.7 mL of H2/g of TVSMethane (DF)43.4 mL of CH4/g of TVS[96]
Laminaria japonicaHydrogen (DF)115.2 mL of H2/gMethane (DF)329.8 mL of CH4/g[97]
Cassava wastewaterHydrogen (DF)54.22 mL of H2/gMethane (DF)164.87 mL of CH4/g[98]
Microalgal biomassHydrogen (DF)135 ± 3.11 mL of H2/g/VSMethane (DF)414 ± 2.45 mL of CH4/g/VS[99]
GlucoseHydrogen (DF)1.20 mmolHydrogen (PF)5.22 mmol[100]
Cheese whey wastewaterHydrogen (DF)2.04 molHydrogen (PF)2.69 mol[101]
Vegetable wasteHydrogen (DF)12.61 mmol H2/dayElectricity (DF)111.76 mW/m2[87]
Fruit juice industry wastewaterHydrogen (DF)1.4 mol H2/mol hexoseElectricity (DF)0.55 W/m2[102]
Corn stover lignocelluloseHydrogen (DF)1.67 mol H2/mol glucoseHydrogen (MEC)1.00 L/L-d[103]
CellobioseHydrogen (DF)1.64 mol H2/mol glucoseHydrogen (MEC)0.96 L/L-d[104]
Distillery spent washHydrogen (DF)39.8 LBioplastic40% dry cell weight[105]
Food wasteHydrogen (DF)3.18 LBioplastic36% dry cell weight[106]
Pea shellsHydrogen (DF)5.2 L of H2 from 4 LBioplastic1685 mg of PHB/L[107]
Food wasteHydrogen (DF)69.94 mmolLipid26.4% dry cell weight[108]
Olive oil mill wastewaterHydrogen (DF)196.2 mL/gBiopolymer8.9% dry cell weight[109]
Molasses wastewaterHydrogen (DF)130.57 mmolEthanol379.3 mg/L[110]
Food wasteBioelectricity85.2 mW/m2Hydrogen (DF)0.91 L[39]
Starch hydrolysateHydrogen (DF)5.40 mmol H2/g of CODHydrogen (PF)10.72 mmol H2/g of COD[111]
SucroseHydrogen (DF)0.98 ± 0.32 mol H2/molHydrogen (PF)4.48 ± 0.23 mol H2/mol[112]
Glucose:xylose (9:1); Microalgae biomassHydrogen (DF)250 mL/L/h; 2.78 mol H2/molMixotropic microalgae cultivation205 mL/L/h; 1.12 g of biomass/g of COD[113]

5.2. Photobiological Process

The photosynthetic bacteria readily consumed the residual organic fraction (VFAs) [100,101]. Because dark fermentative metabolic intermediates can be effectively used by some PNS bacteria, the integration of the anoxygenic photofermentation process with the dark fermentation process will have the dual advantages of increased H2 production with simultaneous removal of the substrates [114]. Chandra and Venkata Mohan [100] investigated the composition and the survivability of mixed microalgal populations during their growth and the production of photofermentative H2 using glucose and acid-rich effluents generated from the process of dark fermentation. Photofermentation with the acid-rich effluents of glucose had a higher efficiency of H2 production (5.22 mmol H2) than dark fermentation (1.21 mmol H2) with glucose as the carbon source. Green algae such as Chlorella also use carbon-rich organic acid intermediates from dark fermentation to produce H2, particularly when acetate is a viable substrate (Equations (6) and (7)) [115].
Dark fermentation (Stage I):
C6H12O6 + 2H2O → 2CH3COOH + 2CO2 + 4H2
Photoheterotrophy (Stage II):
2CH3COOH + 4H2O → 8H2 + 4CO2
The Equations (6) and (7) define an ideal condition in which all the carbon in the form of substrate is processed in the suitable metabolic pathways and none of the carbon is routed to the formation of biomass or alternative metabolites. However, the photofermentation of acid-rich effluents from the H2 production process is more complex than dark fermentation with respect to the efficiency of processing because of poor light penetration, nutritional requirements of the biocatalyst, maintenance of environmental conditions, inhibition of substrates, and contamination obstacles [116,117]. To overcome these limitations, appropriate light arrangements must be either inside or outside of the bioreactor, sufficient nutrients must be supplemented, optimum temperatures and substrate concentrations must be maintained, and the bioreactors must be enclosed systems for ease of sterilization.

5.3. Biodegradable Plastics

The VFA-rich effluents generated from dark fermentation are a potential substrate for the production of bioplastics, such as polyhydroxyalkanoates (PHA) and polyhydroxybutyrates (PHB). The PHAs are a biodegradable biopolyester of hydroxyalkanoates that are produced under extra carbon and nutrient-deprived circumstances and that accumulate as cellular reserve storage material [105,106]. The biopolyesters are deposited as water-insoluble, cytoplasmic micro-sized inclusions in bacterial cells when excess carbon is available and when other nutrients are growth limiting. In general, the PHAs are produced using pure microbial cultures with synthetic substrates (e.g., acetate and butyrate, among others), which is not a cost-effective method of production. The VFAs are simple substrates with a low number of carbon atoms, and the synthesis of PHA requires fewer metabolic enzymes than those of the glycolysis and β-oxidation pathways [106]. The production of PHBs from individual fatty acids (e.g., acetate and butyrate, among others) and acid-rich effluents from dark fermentation was reported for an anoxic microenvironment that used a mixed culture as the biocatalyst [118]. Reddy et al. [106] investigated the production of bioplastics (PHA) using B. tequilensis in aerobic conditions with synthetic acids (SA) and acid-rich effluents of food waste (AFW) as substrates, which were collected from bioreactors producing H2 with dark fermentation. The synthesis of PHAs was higher with SA (59% dry cell weight) than with AFW (36% dry cell weight). They also reported on the presence of a copolymer (P(3HB-co-3HV)) with varying amounts of hydroxy butyrate (HB, 80%–90%) and hydroxy valerate (HV, 10%–15%) for both substrates. Accordingly, the use of the acid-rich effluents from H2 producing reactors as substrates contributed to a significant reduction in the production costs of both the H2 and the PHA embedded with the waste valorization. The entire process was more economically viable when the production of bioplastics was coupled with the production of H2 and their effluents were used for methanogenesis [107].

5.4. Electrically Driven Biohydrogenesis from Acid-Rich Effluents

In recent years, the integration of MECs with other bioprocesses has also received considerable attention [103,119]. As an alternative electrically driven process of H2 production, the MECs facilitate the transformation of biodegradable materials into H2 with an external voltage applied. Indeed, the MEC process was feasible to generate H2 in association with simultaneous wastewater treatment for a wide variety of soluble organic substances [104,120]. A two-stage process was used to convert the acid-rich dark fermentation effluents into substrates for additional H2 production (Figure 5) [103,104]. Babu et al. [104] investigated the feasibility of integrating the MEC process with the dark fermentation process to use the acid-rich effluents for additional H2 recovery. For this integration, the MECs were operated with a small range of varying applied potential (0.2, 0.5, 0.6, 0.8, and 1.0 V) and with acid-rich effluents (concentration of 3000 mg/L) using an anaerobic mixed consortium as a biocatalyst. The maximum hydrogen production rate (HPR) and the cumulative hydrogen production (CHP) were 0.53 mmol/h and 3.6 mmol, respectively, with 49.8% of the VFAs utilized at 0.6 V. With a high substrate conversion efficiency (90%), a two-stage approach, i.e., MECs integrated with dark fermentation, could be a viable option to achieve higher substrate conversion efficiency and H2 yield [44].

5.5. Bioaugmentation

Many biotic and abiotic factors (e.g., microbial physiology and concentration and composition of substrates) also affect the overall yield of the dark fermentative H2 production process with mixed cultures. With the reactor in operation, higher substrate concentrations lead to an accumulation of VFAs and a decrease in system pH (˂4.0), which results in the inhibition of the H2 production process. In an effort to improve the process capability with higher substrate conversion efficiencies, the addition of the desired microbial strains to a native microbial community would be a practical option to overcome the inhibition in the process [121]. These bioaugmentation strategies used single or mixed native microflora with the acidogenic consortia [122], fermentative H2 producing bacteria [123], and C. acetobutylicum [124] to increase H2 production efficiency. Moreover, similar studies were performed to recover the start-up of a bioreactor [125], to boost reactor performance [126], and to protect the native microbial community against problems in the process [121], which indicated that the bioaugmentation strategy was effective to increase H2 production. In some cases, however, the bioaugmented microbial flora might fail to compete with the native population, most likely because of inappropriate operating conditions, failure of substrate utilization, and type and/or diversity of the native microbial population in the system [127,128,129].
Ijms 16 08266 g005 1024
Figure 5. Schematic illustration of microbial electrolysis cells (MECs) integrated with the dark fermentation process for higher H2 yield (A: anode; C: cathode; Biofilm: electrochemically active mixed microbial population). Green, orange, brown, and blue symbols represent a mixed microbial population. In stage 1, initially, complex substrates were used for H2 production in dark fermentation, and in stage 2, acid-rich effluents were used as substrates in MECs for further H2 production.
Figure 5. Schematic illustration of microbial electrolysis cells (MECs) integrated with the dark fermentation process for higher H2 yield (A: anode; C: cathode; Biofilm: electrochemically active mixed microbial population). Green, orange, brown, and blue symbols represent a mixed microbial population. In stage 1, initially, complex substrates were used for H2 production in dark fermentation, and in stage 2, acid-rich effluents were used as substrates in MECs for further H2 production.
Ijms 16 08266 g005

5.6. Utilization of Organic Wastes as a Fermentable Substrate

With rapid urbanization and industrialization, waste management is at the forefront as a major human health and environmental concern [42], and improper waste management increases GHG emissions, which contribute to climate change. Moreover, because many effluents and wastes from foods and food processing and industries that use paper, dairy, cellulosic, and glycerol require a high chemical and biological oxygen demand [130], they potentially threaten the aquatic fauna [131]. For practical and economical aspects, the use of carbon-rich wastes/wastewater as fermentable substrates is an attractive and promising approach for H2 production, which may solve the dual purpose of waste disposal and clean energy generation [132]. This approach would greatly reduce the H2 production processing costs, when compared with chemical and electrolytic processes [133]. The fermentable waste contains biodegradable organic materials to yield a net positive energy, which remains valid for thermophilic fermentative H2 production, although this process requires additional energy for the heating of the substrates and the reactor [16]. Moreover, bioreactors may be installed at or proximal to waste generation sites, which further increases the economic viability of the process [62]. Therefore, a variety of wastes could be used as potentially fermentable substrates for H2 production.

5.7. Pretreatment of Substrates and Biocatalysts

Complex substrates/feedstocks are not the preferred substrates for H2 producing biocatalysts and are a challenging bottleneck to the development of biological pathways for H2 production. To transform nonutilizable substances into fermentable substrates, many pretreatment processes were assessed to make complex substrates into simple ones, based on the types of substrate available [134]. Primarily, pretreatment methods are classified into four major groups: physical (mechanical pretreatment, extrusion, and pyrolysis), physicochemical (steam explosion, ammonia fiber explosion, CO2 explosion, liquid hot water, wet oxidation, sonification, and microwave-based pretreatment), chemical (ozonolysis, acid hydrolysis, alkaline hydrolysis, oxidative delignification, organosolvation, and ionic liquids), and biological (enzymatic hydrolysis) pretreatments. Among these pretreatments, the physicochemical and chemical treatments are the most efficient [135].
The feasibility of H2 production using a mixed microbial population is likely to be restricted because of H2 consumption by methanogens. Therefore, pretreatment of the biocatalyst parent culture may be beneficial for shifting the metabolic pathways to increase acidogenesis, and to inhibiting methanogenesis to improve the H2 production yield with prevention of competitive growth and coexistence of other H2 consuming microorganisms [60,79]. The different biocatalyst pretreatment methods include heat shock (temperature, >80 °C), chemical methods (to inhibit specific metabolic functions; 2-bromoethanesulfonic acid, acetylene, Na2SO4, fluvastatin, chloroform, and iodopropane), acid shock (pH < 4), alkaline shock (pH > 9), an oxygen shock method (oxygen/air), load shock (higher substrate concentration), infrared irradiation, and freezing and thawing (−25 °C for 24 h, followed by a 5 h thaw at 30 °C). The different functional properties of ozone (ozone bubbles) and microwave irradiation methods were evaluated. Indeed, the pretreatments applied to the parent inoculum facilitated the selective enrichment of acidogenic bacteria capable of producing H2 as the end product with the simultaneous prevention of hydrogenotrophic methanogens [136]. Because of the physiological differences between the H2 producing acidogenic bacteria and the H2 consuming bacteria (methanogens), the pretreatment of biocatalysts can also provide a fundamental basis for the development of a H2 production system [60,137].

6. Conclusions

The multidisciplinary fermentation processes used for the production of H2 were numerous, and a variety of substrates were examined. The individual processes possess their own inherent limitations, such as low substrate conversion efficiency, accumulation of VFAs as carbon-rich acid intermediates, and change in system redox conditions and buffering capacity. Thus, to overcome the potential limiting factors and to improve the efficiency of the H2 production process, an understanding of the mechanisms of H+ reduction, functional roles of membrane components, composition of the communities, development of cultures, and design and development of competent bioreactors are the critical areas for both photo- and dark fermentation processes. The initial research on H2 was typically confined to the use of pure cultures as a biocatalyst, and the selection of the biocatalyst depends primarily on the type of fermentable substrates. There is strong consensus that using a mixed microbial population as a biocatalyst is a favorable and practical choice to scale up the technology of H2 production, primarily with wastewater as the substrate (carbon source). Additionally, mixed cultures are typically preferred because of the operational ease, stability, diverse biochemical functions, and probability of using a wide variety of substrates. The optimization of the process parameters is clearly necessary to scale up the technology. The residual organic fraction as a soluble fermentation product after acidogenesis is one of the key limiting factors that requires considerable attention. The approaches with integration that use the acid-rich reactor effluents with the simultaneous recovery of energy must be efficient and completely established for the commercialization of the process to be economically feasible.
In conclusion, the basic and applied research on H2 production provides additional insight into the process for a better understanding to establish an optimized environment. Although various novel approaches are anticipated in future years to overcome some of the persistent problems, biological H2 production technology requires a multidisciplinary approach for the process to be eco-friendly and economically feasible.

Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (2014R1A2A2A01006765) and the Kyungpook National University Research Fund 2014 to Dong-Woo Lee.

Author Contributions

Kuppam Chandrasekhar, Yong-Jik Lee, and Dong-Woo Lee, designed the research, drew the figures, and wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Goldemberg, J. nmental and ecological dimensions of biofuels. In. In Proceedings of the Conference on the Ecological Dimensions of Biofuels, Washington, DC, USA, 10 March 2008.
  2. Venkata Mohan, S.; Pandey, A. Biohydrogen production: An introduction. In Biohydrogen; Pandey, A., Chang, J.-S., Hallenbeck, P.C., Larroche, C., Eds.; Elsevier: Amsterdam, The Netherlands, 2013; pp. 1–24. [Google Scholar]
  3. Lee, S.J.; Lee, S.J.; Lee, D.W. Design and development of synthetic microbial platform cells for bioenergy. Front. Microbiol. 2013, 4, 92. [Google Scholar] [PubMed]
  4. Nouni, M.R. Hydrogen energy and fuel cell technology: Recent developments and future prospects in india. Renew. Energy Akshay Urja 2012, 5, 10–14. [Google Scholar]
  5. Bockris, J.O.M. The origin of ideas on a hydrogen economy and its solution to the decay of the environment. Int. J. Hydrog. Energy 2002, 27, 731–740. [Google Scholar] [CrossRef]
  6. Christopher, K.; Dimitrios, R. A review on exergy comparison of hydrogen production methods from renewable energy sources. Energy Environ. Sci. 2012, 5, 6640–6651. [Google Scholar] [CrossRef]
  7. Lipman, T. An overview of hydrogen production and storage systems with renewable hydrogen case studies. In Energy Efficiency and Renewable Energy Fuel Cell Technologies Program (US DOE Grant DE-FC3608GO18111A000); Clean Energy States Alliance: Oakland, CA, USA, 2011. [Google Scholar]
  8. Jong, W.D. Sustainable hydrogen production by thermochemical biomass processing. Chapter 2008, 6, 185–225. [Google Scholar]
  9. Chen, W.H.; Syu, Y.J. Hydrogen production from water gas shift reaction in a high gravity (Higee) environment using a rotating packed bed. Int. J. Hydrog. Energy 2010, 35, 10179–10189. [Google Scholar] [CrossRef]
  10. Hydrogen generation market-by merchant & captive type, distributed & centralized generation. Report Code: EP 1708. Available online: http://www.marketsandmarkets.com/Market-Reports/hydrogen-generation-market-494.html (accessed on 12 April 2015).
  11. Hallenbeck, P.C.; Benemann, J.R. Biological hydrogen production; fundamentals and limiting processes. Int. J. Hydrog. Energy 2002, 27, 1185–1193. [Google Scholar] [CrossRef]
  12. Hallenbeck, P. Hydrogen production by cyanobacteria. In Microbial Technologies in Advanced Biofuels Production; Hallenbeck, P.C., Ed.; Springer: New York, NY, USA, 2012; pp. 15–28. [Google Scholar]
  13. Boichenko, V.A.; Greenbaum, E.; Seibert, M. Hydrogen production by photosynthetic microorganisms. In Molecular to Global Photosynthesis; Imperial College Press: London, UK, 2004; pp. 397–451. [Google Scholar]
  14. Das, D. Advances in biohydrogen production processes: An approach towards commercialization. Int. J. Hydrog. Energy 2009, 34, 7349–7357. [Google Scholar] [CrossRef]
  15. Mullai, P.; Rene, E.R.; Sridevi, K. Biohydrogen production and kinetic modeling using sediment microorganisms of pichavaram mangroves, India. Biomed. Res. Int. 2013, 2013. [Google Scholar] [CrossRef] [PubMed]
  16. Angenent, L.T.; Karim, K.; Al-Dahhan, M.H.; Domiguez-Espinosa, R. Production of bioenergy and biochemicals from industrial and agricultural wastewater. Trends Biotechnol. 2004, 22, 477–485. [Google Scholar] [CrossRef] [PubMed]
  17. Wang, J.L.; Wan, W. Factors influencing fermentative hydrogen production: A review. Int. J. Hydrog. Energy 2009, 34, 799–811. [Google Scholar] [CrossRef]
  18. Venkata Mohan, S.; Chandrasekhar, K.; Chiranjeevi, P.; Babu, P.S. Biohydrogen production from wastewater. In Biohydrogen; Pandey, A., Chang, J.-S., Hallenbeck, P.C., Larroche, C., Eds.; Elsevier: Amsterdam, The Netherlands, 2013; pp. 223–257. [Google Scholar]
  19. Melis, A.; Zhang, L.; Forestier, M.; Ghirardi, M.L.; Seibert, M. Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green Alga Chlamydomonas reinhardtii. Plant Physiol. 2000, 122, 127–136. [Google Scholar] [CrossRef] [PubMed]
  20. Jones, C.S.; Mayfield, S.P. Algae biofuels: Versatility for the future of bioenergy. Curr. Opin. Biotechnol. 2012, 23, 346–351. [Google Scholar] [CrossRef] [PubMed]
  21. Eroglu, E.; Melis, A. Photobiological hydrogen production: Recent advances and state of the art. Bioresour. Technol. 2011, 102, 8403–8413. [Google Scholar] [CrossRef] [PubMed]
  22. Tekucheva, D.N.; Tsygankov, A.A. Coupled biological hydrogen-producing systems: A review. Prikl. Biokhimiia Mikrobiol. 2012, 48, 357–375. [Google Scholar]
  23. Huesemann, M.H.; Hausmann, T.S.; Carter, B.M.; Gerschler, J.J.; Benemann, J.R. Hydrogen generation through indirect biophotolysis in batch cultures of the nonheterocystous nitrogen-fixing cyanobacterium plectonema boryanum. Appl. Biochem. Biotechnol. 2010, 162, 208–220. [Google Scholar] [CrossRef] [PubMed]
  24. Bandyopadhyay, A.; Stöckel, J.; Min, H.; Sherman, L.A.; Pakrasi, H.B. High rates of photobiological H2 production by a cyanobacterium under aerobic conditions. Nat. Commun. 2010, 1, 139. [Google Scholar] [CrossRef] [PubMed]
  25. Melnicki, M.R.; Pinchuk, G.E.; Hill, E.A.; Kucek, L.A.; Fredrickson, J.K.; Konopka, A.; Beliaev, A.S. Sustained H2 production driven by photosynthetic water splitting in a unicellular cyanobacterium. MBio 2012, 3, e00197–e00112. [Google Scholar] [CrossRef] [PubMed]
  26. Kosourov, S.N.; Ghirardi, M.L.; Seibert, M. A truncated antenna mutant of chlamydomonas reinhardtii can produce more hydrogen than the parental strain. Int. J. Hydrog. Energy 2011, 36, 2044–2048. [Google Scholar] [CrossRef]
  27. Scoma, A.; Krawietz, D.; Faraloni, C.; Giannelli, L.; Happe, T.; Torzillo, G. Sustained H2 production in a chlamydomonas reinhardtii D1 protein mutant. J. Biotechnol. 2012, 157, 613–619. [Google Scholar] [CrossRef] [PubMed]
  28. Esquível, M.G.; Amaro, H.M.; Pinto, T.S.; Fevereiro, P.S.; Malcata, F.X. Efficient H2 production via chlamydomonas reinhardtii. Trends Biotechnol. 2011, 29, 595–600. [Google Scholar] [CrossRef] [PubMed]
  29. Ducat, D.C.; Sachdeva, G.; Silver, P.A. Rewiring hydrogenase-dependent redox circuits in cyanobacteria. Proc. Natl. Acad. Sci. USA 2011, 108, 3941–3946. [Google Scholar] [CrossRef] [PubMed]
  30. Basak, N.; Das, D. The prospect of purple non-sulfur (PNS) photosynthetic bacteria for hydrogen production: The present state of the art. World J. Microbiol. Biotechnol. 2007, 23, 31–42. [Google Scholar] [CrossRef]
  31. Azwar, M.Y.; Hussain, M.A.; Abdul-Wahab, A.K. Development of biohydrogen production by photobiological, fermentation and electrochemical processes: A review. Renew. Sustain. Energy Rev. 2014, 31, 158–173. [Google Scholar] [CrossRef]
  32. Brentner, L.B.; Jordan, P.A.; Zimmerman, J.B. Challenges in developing biohydrogen as a sustainable energy source: Implications for a research agenda. Environ. Sci. Technol. 2010, 44, 2243–2254. [Google Scholar] [CrossRef] [PubMed]
  33. Koku, H.; Eroğlu, İ.; Gündüz, U.; Yücel, M.; Türker, L. Aspects of the metabolism of hydrogen production by rhodobacter sphaeroides. Int. J. Hydrog. Energy 2002, 27, 1315–1329. [Google Scholar] [CrossRef]
  34. Ghosh, D.; Sobro, I.F.; Hallenbeck, P.C. Optimization of the hydrogen yield from single-stage photofermentation of glucose by rhodobacter capsulatus jp91 using response surface methodology. Bioresour. Technol. 2012, 123, 199–206. [Google Scholar] [CrossRef] [PubMed]
  35. Ghosh, D.; Tourigny, A.; Hallenbeck, P.C. Near stoichiometric reforming of biodiesel derived crude glycerol to hydrogen by photofermentation. Int. J. Hydrog. Energy 2012, 37, 2273–2277. [Google Scholar] [CrossRef]
  36. Ghosh, D.; Sobro, I.F.; Hallenbeck, P.C. Stoichiometric conversion of biodiesel derived crude glycerol to hydrogen: Response surface methodology study of the effects of light intensity and crude glycerol and glutamate concentration. Bioresour. Technol. 2012, 106, 154–160. [Google Scholar] [CrossRef] [PubMed]
  37. Hallenbeck, P.C.; Ghosh, D. Advances in fermentative biohydrogen production: The way forward? Trends Biotechnol. 2009, 27, 287–297. [Google Scholar] [CrossRef] [PubMed]
  38. Chandrasekhar, K.; Venkata Mohan, S. Bio-electrohydrolysis as a pretreatment strategy to catabolize complex food waste in closed circuitry: Function of electron flux to enhance acidogenic biohydrogen production. Int. J. Hydrog. Energy 2014, 39, 11411–11422. [Google Scholar] [CrossRef]
  39. Chandrasekhar, K.; Venkata Mohan, S. Induced catabolic bio-electrohydrolysis of complex food waste by regulating external resistance for enhancing acidogenic biohydrogen production. Bioresour. Technol. 2014, 165, 372–382. [Google Scholar] [CrossRef] [PubMed]
  40. Vardar-Schara, G.; Maeda, T.; Wood, T.K. Metabolically engineered bacteria for producing hydrogen via fermentation. Microb. Biotechnol. 2008, 1, 107–125. [Google Scholar] [CrossRef] [PubMed]
  41. Kraemer, J.; Bagley, D. Improving the yield from fermentative hydrogen production. Biotechnol. Lett. 2007, 29, 685–695. [Google Scholar] [CrossRef] [PubMed]
  42. Venkata Mohan, S.; Chandrasekhar, K. Solid phase microbial fuel cell (SMFC) for harnessing bioelectricity from composite food waste fermentation: Influence of electrode assembly and buffering capacity. Bioresour. Technol. 2011, 102, 7077–7085. [Google Scholar]
  43. Nath, K.; Das, D. Modeling and optimization of fermentative hydrogen production. Bioresour. Technol. 2011, 102, 8569–8581. [Google Scholar] [CrossRef] [PubMed]
  44. Cheng, S.; Logan, B.E. Sustainable and efficient biohydrogen production via electrohydrogenesis. Proc. Natl. Acad. Sci. USA 2007, 104, 18871–18873. [Google Scholar] [CrossRef] [PubMed]
  45. Rozendal, R.A.; Hamelers, H.V.M.; Molenkamp, R.J.; Buisman, C.J.N. Performance of single chamber biocatalyzed electrolysis with different types of ion exchange membranes. Water Res. 2007, 41, 1984–1994. [Google Scholar] [CrossRef] [PubMed]
  46. Hallenbeck, P.C. Microbial paths to renewable hydrogen production. Biofuels 2011, 2, 285–302. [Google Scholar] [CrossRef]
  47. Clauwaert, P.; Verstraete, W. Methanogenesis in membraneless microbial electrolysis cells. Appl. Microbiol. Biotechnol. 2009, 82, 829–836. [Google Scholar] [CrossRef] [PubMed]
  48. Yokoyama, H.; Ohmori, H.; Waki, M.; Ogino, A.; Tanaka, Y. Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora. J. Biosci. Bioeng. 2009, 107, 64–66. [Google Scholar] [CrossRef] [PubMed]
  49. Levin, D.B.; Pitt, L.; Love, M. Biohydrogen production: Prospects and limitations to practical application. Int. J. Hydrog. Energy 2004, 29, 173–185. [Google Scholar] [CrossRef]
  50. Tang, G.-L.; Huang, J.; Sun, Z.-J.; Tang, Q.-Q.; Yan, C.-H.; Liu, G.-Q. Biohydrogen production from cattle wastewater by enriched anaerobic mixed consortia: Influence of fermentation temperature and ph. J. Biosci. Bioeng. 2008, 106, 80–87. [Google Scholar] [CrossRef] [PubMed]
  51. Li, C.L.; Fang, H.H.P. Fermentative hydrogen production from wastewater and solid wastes by mixed cultures. Crit. Rev. Environ. Sci. Technol. 2007, 37, 1–39. [Google Scholar] [CrossRef]
  52. Lin, C.-Y.; Chang, C.-C.; Hung, C.-H. Fermentative hydrogen production from starch using natural mixed cultures. Int. J. Hydrog. Energy 2008, 33, 2445–2453. [Google Scholar] [CrossRef]
  53. Maru, B.T.; Bielen, A.A.M.; Constantí, M.; Medina, F.; Kengen, S.W.M. Glycerol fermentation to hydrogen by thermotoga maritima: Proposed pathway and bioenergetic considerations. Int. J. Hydrog. Energy 2013, 38, 5563–5572. [Google Scholar] [CrossRef]
  54. De Vrije, T.C.P. Dark hydrogen fermentations. In Biomethane & Biohydrogen; Reith, J.H., Wijffels, R.H., Barten, H., Eds.; Smiet Offset: Hague, The Netherlands, 2003; pp. 103–123. [Google Scholar]
  55. Kiran Kumar, A.; Venkateswar Reddy, M.; Chandrasekhar, K.; Srikanth, S.; Venkata Mohan, S. Endocrine disruptive estrogens role in electron transfer: Bio-electrochemical remediation with microbial mediated electrogenesis. Bioresour. Technol. 2012, 104, 547–556. [Google Scholar]
  56. Venkata Mohan, S.; Chandrasekhar, K. Self-induced bio-potential and graphite electron accepting conditions enhances petroleum sludge degradation in bio-electrochemical system with simultaneous power generation. Bioresour. Technol. 2011, 102, 9532–9541. [Google Scholar]
  57. Antonopoulou, G.; Stamatelatou, K.; Venetsaneas, N.; Kornaros, M.; Lyberatos, G. Biohydrogen and methane production from cheese whey in a two-stage anaerobic process. Indust. Eng. Chem. Res. 2008, 47, 5227–5233. [Google Scholar] [CrossRef]
  58. Fan, Y.; Li, C.; Lay, J.-J.; Hou, H.; Zhang, G. Optimization of initial substrate and pH levels for germination of sporing hydrogen-producing anaerobes in cow dung compost. Bioresour. Technol. 2004, 91, 189–193. [Google Scholar] [CrossRef] [PubMed]
  59. Van Ginkel, S.; Sung, S.; Lay, J.J. Biohydrogen production as a function of ph and substrate concentration. Environ. Sci. Technol. 2001, 35, 4726–4730. [Google Scholar]
  60. Zhu, H.; Béland, M. Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge. Int. J. Hydrog. Energy 2006, 31, 1980–1988. [Google Scholar] [CrossRef]
  61. Özgür, E.; Afsar, N.; de Vrije, T.; Yücel, M.; Gündüz, U.; Claassen, P.A.M.; Eroglu, I. Potential use of thermophilic dark fermentation effluents in photofermentative hydrogen production by rhodobacter capsulatus. J. Clean. Prod. 2010, 18, S23–S28. [Google Scholar] [CrossRef]
  62. Kapdan, I.K.; Kargi, F. Bio-hydrogen production from waste materials. Enzym. Microb. Technol. 2006, 38, 569–582. [Google Scholar] [CrossRef]
  63. Lin, C.Y.; Lay, C.H. Effects of carbonate and phosphate concentrations on hydrogen production using anaerobic sewage sludge microflora. Int. J. Hydrog. Energy 2004, 29, 275–281. [Google Scholar] [CrossRef]
  64. Wang, B.; Wan, W.; Wang, J. Effect of ammonia concentration on fermentative hydrogen production by mixed cultures. Bioresour. Technol. 2009, 100, 1211–1213. [Google Scholar] [CrossRef] [PubMed]
  65. Chen, S.-D.; Lee, K.-S.; Lo, Y.-C.; Chen, W.-M.; Wu, J.-F.; Lin, C.-Y.; Chang, J.-S. Batch and continuous biohydrogen production from starch hydrolysate by clostridium species. Int. J. Hydrog. Energy 2008, 33, 1803–1812. [Google Scholar] [CrossRef]
  66. Redwood, M.D.; Macaskie, L.E. A two-stage, two-organism process for biohydrogen from glucose. Int. J. Hydrog. Energy 2006, 31, 1514–1521. [Google Scholar] [CrossRef]
  67. Salerno, M.B.; Park, W.; Zuo, Y.; Logan, B.E. Inhibition of biohydrogen production by ammonia. Water Res. 2006, 40, 1167–1172. [Google Scholar] [CrossRef] [PubMed]
  68. Lin, C.Y.; Lay, C.H. Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora. Int. J. Hydrog. Energy 2004, 29, 41–45. [Google Scholar] [CrossRef]
  69. Yadvika; Santosh; Sreekrishnan, T.R.; Kohli, S.; Rana, V. Enhancement of biogas production from solid substrates using different techniques-a review. Bioresour. Technol. 2004, 95, 1–10. [Google Scholar]
  70. Lin, C.Y.; Shei, S.H. Heavy metal effects on fermentative hydrogen production using natural mixed microflora. Int. J. Hydrog. Energy 2008, 33, 587–593. [Google Scholar] [CrossRef]
  71. Tsaousis, A.D.; Nývltová, E.; Šuták, R.; Hrdý, I.; Tachezy, J. A nonmitochondrial hydrogen production in naegleria gruberi. Genome Biol. Evol. 2014, 6, 792–799. [Google Scholar] [CrossRef] [PubMed]
  72. Lee, Y.J.; Miyahara, T.; Noike, T. Effect of iron concentration on hydrogen fermentation. Bioresour. Technol. 2001, 80, 227–231. [Google Scholar] [CrossRef] [PubMed]
  73. Srikanth, S.; Venkata Mohan, S. Regulatory function of divalent cations in controlling the acidogenic biohydrogen production process. RSC Adv. 2012, 2, 6576–6589. [Google Scholar] [CrossRef]
  74. Karadag, D.; Puhakka, J.A. Enhancement of anaerobic hydrogen production by iron and nickel. Int. J. Hydrog. Energy 2010, 35, 8554–8560. [Google Scholar] [CrossRef]
  75. Zhang, Y.; Liu, G.; Shen, J. Hydrogen production in batch culture of mixed bacteria with sucrose under different iron concentrations. Int. J. Hydrog. Energy 2005, 30, 855–860. [Google Scholar] [CrossRef]
  76. Zhang, Y.; Shen, J. Effect of temperature and iron concentration on the growth and hydrogen production of mixed bacteria. Int. J. Hydrog. Energy 2006, 31, 441–446. [Google Scholar] [CrossRef]
  77. Chang, F.Y.; Lin, C.Y. Calcium effect on fermentative hydrogen production in an anaerobic up-flow sludge blanket system. Water Sci. Technol. 2006, 54, 105–112. [Google Scholar] [CrossRef] [PubMed]
  78. Hawkes, F.R.; Hussy, I.; Kyazze, G.; Dinsdale, R.; Hawkes, D.L. Continuous dark fermentative hydrogen production by mesophilic microflora: Principles and progress. Int. J. Hydrog. Energy 2007, 32, 172–184. [Google Scholar] [CrossRef]
  79. Venkata Mohan, S. Waste to renewable energy: A sustainable and green approach towards production of biohydrogen by acidogenic fermentation. In Sustainable Biotechnology; Singh, O.V., Harvey, S.P., Eds.; Springer: Amsterdam, The Netherlands, 2010; pp. 129–164. [Google Scholar]
  80. Vijaya Bhaskar, Y.; Venkata Mohan, S.; Sarma, P.N. Effect of substrate loading rate of chemical wastewater on fermentative biohydrogen production in biofilm configured sequencing batch reactor. Bioresour. Technol. 2008, 99, 6941–6948. [Google Scholar]
  81. Ren, N.; Chen, Z.; Wang, A.; Hu, D. Removal of organic pollutants and analysis of mlss–cod removal relationship at different hrts in a submerged membrane bioreactor. Int. Biodeterior. Biodegrad. 2005, 55, 279–284. [Google Scholar] [CrossRef]
  82. Chen, W.-H.; Sung, S.; Chen, S.-Y. Biological hydrogen production in an anaerobic sequencing batch reactor: pH and cyclic duration effects. Int. J. Hydrog. Energy 2009, 34, 227–234. [Google Scholar] [CrossRef]
  83. Nath, K.; Das, D. Improvement of fermentative hydrogen production: Various approaches. Appl. Microbiol. Biotechnol. 2004, 65, 520–529. [Google Scholar] [CrossRef] [PubMed]
  84. Van Groenestijn, J.W.; Hazewinkel, J.H.O.; Nienoord, M.; Bussmann, P.J.T. Energy aspects of biological hydrogen production in high rate bioreactors operated in the thermophilic temperature range. Int. J. Hydrog. Energy 2002, 27, 1141–1147. [Google Scholar]
  85. Abreu, A.A.; Karakashev, D.; Angelidaki, I.; Sousa, D.Z.; Alves, M.M. Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures. Biotechnol. Biofuels 2012, 5, 6. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  86. Abo-Hashesh, M.; Hallenbeck, P.C. Microaerobic dark fermentative hydrogen production by the photosynthetic bacterium, rhodobacter capsulatus jp91. Int. J. Low Carbon Technol. 2012, 7, 97–103. [Google Scholar] [CrossRef]
  87. Mohanakrishna, G.; Venkata Mohan, S.; Sarma, P.N. Utilizing acid-rich effluents of fermentative hydrogen production process as substrate for harnessing bioelectricity: An integrative approach. Int. J. Hydrog. Energy 2010, 35, 3440–3449. [Google Scholar] [CrossRef]
  88. Gadhamshetty, V.; Sukumaran, A.; Nirmalakhandan, N. Review: Photoparameters in photofermentative biohydrogen production. J. Biosci. Bioeng. 2011, 41, 1–51. [Google Scholar]
  89. Keskin, T.; Abo-Hashesh, M.; Hallenbeck, P.C. Photofermentative hydrogen production from wastes. Bioresour. Technol. 2011, 102, 8557–8568. [Google Scholar] [CrossRef] [PubMed]
  90. Laurinavichene, T.V.; Belokopytov, B.F.; Laurinavichius, K.S.; Khusnutdinova, A.N.; Seibert, M.; Tsygankov, A.A. Towards the integration of dark- and photo-fermentative waste treatment. 4. Repeated batch sequential dark- and photofermentation using starch as substrate. Int. J. Hydrog. Energy 2012, 37, 8800–8810. [Google Scholar] [CrossRef]
  91. Laurinavichene, T.V.; Belokopytov, B.F.; Laurinavichius, K.S.; Tekucheva, D.N.; Seibert, M.; Tsygankov, A.A. Towards the integration of dark- and photo-fermentative waste treatment. 3. Potato as substrate for sequential dark fermentation and light-driven h2 production. Int. J. Hydrog. Energy 2010, 35, 8536–8543. [Google Scholar] [CrossRef]
  92. Belokopytov, B.F.; Laurinavichius, K.S.; Laurinavichene, T.V.; Ghirardi, M.L.; Seibert, M.; Tsygankov, A.A. Towards the integration of dark- and photo-fermentative waste treatment. 2. Optimization of starch-dependent fermentative hydrogen production. Int. J. Hydrog. Energy 2009, 34, 3324–3332. [Google Scholar] [CrossRef]
  93. Cheng, X.-Y.; Li, Q.; Liu, C.-Z. Coproduction of hydrogen and methane via anaerobic fermentation of cornstalk waste in continuous stirred tank reactor integrated with up-flow anaerobic sludge bed. Bioresour. Technol. 2012, 114, 327–333. [Google Scholar] [CrossRef] [PubMed]
  94. Cheng, H.-H.; Whang, L.-M.; Wu, C.-W.; Chung, M.-C. A two-stage bioprocess for hydrogen and methane production from rice straw bioethanol residues. Bioresour. Technol. 2012, 113, 23–29. [Google Scholar] [CrossRef] [PubMed]
  95. Chuang, Y.-S.; Lay, C.-H.; Sen, B.; Chen, C.-C.; K, G.; Wu, J.-H.; Lin, C.-S.; Lin, C.-Y. Biohydrogen and biomethane from water hyacinth (eichhornia crassipes) fermentation: Effects of substrate concentration and incubation temperature. Int. J. Hydrog. Energy 2011, 36, 14195–14203. [Google Scholar] [CrossRef]
  96. Cheng, J.; Xie, B.; Zhou, J.; Song, W.; Cen, K. Cogeneration of H2 and CH4 from water hyacinth by two-step anaerobic fermentation. Int. J. Hydrog. Energy 2010, 35, 3029–3035. [Google Scholar] [CrossRef]
  97. Jung, K.-W.; Kim, D.-H.; Shin, H.-S. Continuous fermentative hydrogen and methane production from laminaria japonica using a two-stage fermentation system with recycling of methane fermented effluent. Int. J. Hydrog. Energy 2012, 37, 15648–15657. [Google Scholar] [CrossRef]
  98. Intanoo, P.; Rangsanvigit, P.; Malakul, P.; Chavadej, S. Optimization of separate hydrogen and methane production from cassava wastewater using two-stage upflow anaerobic sludge blanket reactor (UASB) system under thermophilic operation. Bioresour. Technol. 2014, 173, 256–265. [Google Scholar] [CrossRef] [PubMed]
  99. Wieczorek, N.; Kucuker, M.A.; Kuchta, K. Fermentative hydrogen and methane production from microalgal biomass (chlorella vulgaris) in a two-stage combined process. Appl. Energy 2014, 132, 108–117. [Google Scholar] [CrossRef]
  100. Chandra, R.; Venkata Mohan, S. Microalgal community and their growth conditions influence biohydrogen production during integration of dark-fermentation and photo-fermentation processes. Int. J. Hydrog. Energy 2011, 36, 12211–12219. [Google Scholar] [CrossRef]
  101. Rai, P.; Singh, S.P.; Asthana, R.K. Biohydrogen production from cheese whey wastewater in a two-step anaerobic process. Appl. Biochem. Biotechnol. 2012, 167, 1540–1549. [Google Scholar] [CrossRef] [PubMed]
  102. Gonzalez del Campo, A.; Cañizares, P.; Lobato, J.; Rodrigo, M.A.; Fernandez, F.J. Electricity production by integration of acidogenic fermentation of fruit juice wastewater and fuel cells. Int. J. Hydrog. Energy 2012, 37, 9028–9037. [Google Scholar] [CrossRef]
  103. Lalaurette, E.; Thammannagowda, S.; Mohagheghi, A.; Maness, P.-C.; Logan, B.E. Hydrogen production from cellulose in a two-stage process combining fermentation and electrohydrogenesis. Int. J. Hydrog. Energy 2009, 34, 6201–6210. [Google Scholar] [CrossRef]
  104. Lenin Babu, M.; Venkata Subhash, G.; Sarma, P.N.; Venkata Mohan, S. Bio-electrolytic conversion of acidogenic effluents to biohydrogen: An integration strategy for higher substrate conversion and product recovery. Bioresour. Technol. 2013, 133, 322–331. [Google Scholar] [CrossRef] [PubMed]
  105. Amulya, K.; Venkateswar Reddy, M.; Venkata Mohan, S. Acidogenic spent wash valorization through polyhydroxyalkanoate (PHA) synthesis coupled with fermentative biohydrogen production. Bioresour. Technol. 2014, 158, 336–342. [Google Scholar] [CrossRef] [PubMed]
  106. Venkateswar Reddy, M.; Amulya, K.; Rohit, M.V.; Sarma, P.N.; Venkata Mohan, S. Valorization of fatty acid waste for bioplastics production using bacillus tequilensis: Integration with dark-fermentative hydrogen production process. Int. J. Hydrog. Energy 2014, 39, 7616–7626. [Google Scholar] [CrossRef]
  107. Patel, S.K.S.; Singh, M.; Kumar, P.; Purohit, H.J.; Kalia, V.C. Exploitation of defined bacterial cultures for production of hydrogen and polyhydroxybutyrate from pea-shells. Biomass Bioenergy 2012, 36, 218–225. [Google Scholar] [CrossRef]
  108. Venkata Mohan, S.; Prathima Devi, M. Fatty acid rich effluent from acidogenic biohydrogen reactor as substrate for lipid accumulation in heterotrophic microalgae with simultaneous treatment. Bioresour. Technol. 2012, 123, 627–635. [Google Scholar] [CrossRef] [PubMed]
  109. Ntaikou, I.; Kourmentza, C.; Koutrouli, E.C.; Stamatelatou, K.; Zampraka, A.; Kornaros, M.; Lyberatos, G. Exploitation of olive oil mill wastewater for combined biohydrogen and biopolymers production. Bioresour. Technol. 2009, 100, 3724–3730. [Google Scholar] [CrossRef] [PubMed]
  110. Han, W.; Wang, B.; Zhou, Y.; Wang, D.-X.; Wang, Y.; Yue, L.-R.; Li, Y.-F.; Ren, N.-Q. Fermentative hydrogen production from molasses wastewater in a continuous mixed immobilized sludge reactor. Bioresour. Technol. 2012, 110, 219–223. [Google Scholar] [CrossRef] [PubMed]
  111. Lo, Y.-C.; Chen, S.-D.; Chen, C.-Y.; Huang, T.-I.; Lin, C.-Y.; Chang, J.-S. Combining enzymatic hydrolysis and dark-photo fermentation processes for hydrogen production from starch feedstock: A feasibility study. Int. J. Hydrog. Energy 2008, 33, 5224–5233. [Google Scholar] [CrossRef]
  112. Lo, Y.-C.; Chen, C.-Y.; Lee, C.-M.; Chang, J.-S. Sequential dark-photo fermentation and autotrophic microalgal growth for high-yield and co2-free biohydrogen production. Int. J. Hydrog. Energy 2010, 35, 10944–10953. [Google Scholar] [CrossRef]
  113. Liu, C.-H.; Chang, C.-Y.; Liao, Q.; Zhu, X.; Liao, C.-F.; Chang, J.-S. Biohydrogen production by a novel integration of dark fermentation and mixotrophic microalgae cultivation. Int. J. Hydrog. Energy 2013, 38, 15807–15814. [Google Scholar] [CrossRef]
  114. Mohanakrishna, G.; Venkata Mohan, S. Multiple process integrations for broad perspective analysis of fermentative h2 production from wastewater treatment: Technical and environmental considerations. Appl. Energy 2013, 107, 244–254. [Google Scholar] [CrossRef]
  115. Bala Amutha, K.; Murugesan, A.G. Biological hydrogen production by the algal biomass Chlorella vulgaris MSU 01 strain isolated from pond sediment. Bioresour. Technol. 2011, 102, 194–199. [Google Scholar] [CrossRef] [PubMed]
  116. Ozmihci, S.; Kargi, F. Bio-hydrogen production by photo-fermentation of dark fermentation effluent with intermittent feeding and effluent removal. Int. J. Hydrog. Energy 2010, 35, 6674–6680. [Google Scholar] [CrossRef]
  117. Özkan, E.; Uyar, B.; Özgür, E.; Yücel, M.; Eroglu, I.; Gündüz, U. Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice in outdoor conditions. Int. J. Hydrog. Energy 2012, 37, 2044–2049. [Google Scholar] [CrossRef]
  118. Venkateswar Reddy, M.; Venkata Mohan, S. Influence of aerobic and anoxic microenvironments on polyhydroxyalkanoates (PHA) production from food waste and acidogenic effluents using aerobic consortia. Bioresour. Technol. 2012, 103, 313–321. [Google Scholar] [CrossRef] [PubMed]
  119. Cusick, R.D.; Kiely, P.D.; Logan, B.E. A monetary comparison of energy recovered from microbial fuel cells and microbial electrolysis cells fed winery or domestic wastewaters. Int. J. Hydrog. Energy 2010, 35, 8855–8861. [Google Scholar] [CrossRef]
  120. Wagner, R.C.; Regan, J.M.; Oh, S.-E.; Zuo, Y.; Logan, B.E. Hydrogen and methane production from swine wastewater using microbial electrolysis cells. Water Res. 2009, 43, 1480–1488. [Google Scholar] [CrossRef] [PubMed]
  121. Goud, R.K.; Sarkar, O.; Chiranjeevi, P.; Venkata Mohan, S. Bioaugmentation of potent acidogenic isolates: A strategy for enhancing biohydrogen production at elevated organic load. Bioresour. Technol. 2014, 165, 223–232. [Google Scholar] [CrossRef] [PubMed]
  122. Venkata Mohan, S.; Vijaya Bhaskar, Y.; Murali Krishna, P.; Chandrasekhara Rao, N.; Lalit Babu, V.; Sarma, P.N. Biohydrogen production from chemical wastewater as substrate by selectively enriched anaerobic mixed consortia: Influence of fermentation ph and substrate composition. Int. J. Hydrog. Energy 2007, 32, 2286–2295. [Google Scholar] [CrossRef]
  123. Marone, A.; Massini, G.; Patriarca, C.; Signorini, A.; Varrone, C.; Izzo, G. Hydrogen production from vegetable waste by bioaugmentation of indigenous fermentative communities. Int. J. Hydrog. Energy 2012, 37, 5612–5622. [Google Scholar] [CrossRef]
  124. Wang, A.; Ren, N.; Shi, Y.; Lee, D.-J. Bioaugmented hydrogen production from microcrystalline cellulose using co-culture—clostridium acetobutylicum and ethanoigenens harbinense. Int. J. Hydrog. Energy 2008, 33, 912–917. [Google Scholar] [CrossRef]
  125. Ma, F.; Guo, J.-B.; Zhao, L.-J.; Chang, C.-C.; Cui, D. Application of bioaugmentation to improve the activated sludge system into the contact oxidation system treating petrochemical wastewater. Bioresour. Technol. 2009, 100, 597–602. [Google Scholar] [CrossRef] [PubMed]
  126. Venkata Mohan, S.; Falkentoft, C.; Venkata Nancharaiah, Y.; Sturm, B.S.M.; Wattiau, P.; Wilderer, P.A.; Wuertz, S.; Hausner, M. Bioaugmentation of microbial communities in laboratory and pilot scale sequencing batch biofilm reactors using the tol plasmid. Bioresour. Technol. 2009, 100, 1746–1753. [Google Scholar] [CrossRef] [PubMed]
  127. Bouchez, T.; Patureau, D.; Dabert, P.; Wagner, M.; Delgenès, J.P.; Moletta, R. Successful and unsuccessful bioaugmentation experiments monitored by fluorescent in situ hybridization. Water Sci. Technol. 2000, 41, 61–68. [Google Scholar] [PubMed]
  128. Chandrasekhar, K.; Venkata Mohan, S. Bio-electrochemical remediation of real field petroleum sludge as an electron donor with simultaneous power generation facilitates biotransformation of pah: Effect of substrate concentration. Bioresour. Technol. 2012, 110, 517–525. [Google Scholar] [CrossRef] [PubMed]
  129. Venkata Mohan, S.R.; Devi, M.P.; Reddy, M.; Chandrasekhar, K.; Juwarkar, A.; Sarma, P.N. Bioremediation of petroleum sludge under anaerobic microenvironment: Influence of biostimulation and bioaugmentation. Environ. Eng. Manag. J. 2011, 10, 1609–1616. [Google Scholar]
  130. Chong, M.-L.; Rahim, R.A.; Shirai, Y.; Hassan, M.A. Biohydrogen production by clostridium butyricum eb6 from palm oil mill effluent. Int. J. Hydrog. Energy 2009, 34, 764–771. [Google Scholar] [CrossRef] [Green Version]
  131. Reddy, M.V.; Devi, M.P.; Chandrasekhar, K.; Goud, R.K.; Venkata Mohan, S. Aerobic remediation of petroleum sludge through soil supplementation: Microbial community analysis. J. Hazard. Mater. 2011, 197, 80–87. [Google Scholar] [CrossRef] [PubMed]
  132. Reddy, M.V.; Chandrasekhar, K.; Venkata Mohan, S. Influence of carbohydrates and proteins concentration on fermentative hydrogen production using canteen based waste under acidophilic microenvironment. J. Biotechnol. 2011, 155, 387–395. [Google Scholar] [CrossRef] [PubMed]
  133. Nath, K.; Das, D. Hydrogen from biomass. Curr. Sci. 2003, 85, 265–271. [Google Scholar]
  134. Taherzadeh, M.; Karimi, K. Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: A review. Int. J. Mol. Sci. 2008, 9, 1621–1651. [Google Scholar] [CrossRef] [PubMed]
  135. Ren, N.-Q.; Guo, W.-Q.; Wang, X.-J.; Xiang, W.-S.; Liu, B.-F.; Wang, X.-Z.; Ding, J.; Chen, Z.-B. Effects of different pretreatment methods on fermentation types and dominant bacteria for hydrogen production. Int. J. Hydrog. Energy 2008, 33, 4318–4324. [Google Scholar] [CrossRef]
  136. Venkata Mohan, S.; Goud, R.K. Pretreatment of biocatalyst as viable option for sustained production of biohydrogen from wastewater treatment. In Biogas Production; John Wiley & Sons, Inc.: Hoboken, NJ, USA, 2012; pp. 291–311. [Google Scholar]
  137. Srikanth, S.; Venkata Mohan, S.; Lalit Babu, V.; Sarma, P.N. Metabolic shift and electron discharge pattern of anaerobic consortia as a function of pretreatment method applied during fermentative hydrogen production. Int. J. Hydrog. Energy 2010, 35, 10693–10700. [Google Scholar] [CrossRef]

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Chandrasekhar, K.; Lee, Y.-J.; Lee, D.-W. Biohydrogen Production: Strategies to Improve Process Efficiency through Microbial Routes. Int. J. Mol. Sci. 2015, 16, 8266-8293. https://doi.org/10.3390/ijms16048266

AMA Style

Chandrasekhar K, Lee Y-J, Lee D-W. Biohydrogen Production: Strategies to Improve Process Efficiency through Microbial Routes. International Journal of Molecular Sciences. 2015; 16(4):8266-8293. https://doi.org/10.3390/ijms16048266

Chicago/Turabian Style

Chandrasekhar, Kuppam, Yong-Jik Lee, and Dong-Woo Lee. 2015. "Biohydrogen Production: Strategies to Improve Process Efficiency through Microbial Routes" International Journal of Molecular Sciences 16, no. 4: 8266-8293. https://doi.org/10.3390/ijms16048266

APA Style

Chandrasekhar, K., Lee, Y. -J., & Lee, D. -W. (2015). Biohydrogen Production: Strategies to Improve Process Efficiency through Microbial Routes. International Journal of Molecular Sciences, 16(4), 8266-8293. https://doi.org/10.3390/ijms16048266

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