BAG2 Interferes with CHIP-Mediated Ubiquitination of HSP72
Abstract
:1. Introduction
2. Results
2.1. BAG2 Interferes with CHIP-Mediated Substrate Ubiquitination
2.2. Cellular Aging Is Associated with Increased CHIP and BAG2 Levels
2.3. BAG2 Inhibits CHIP-Mediated HSP72 Ubiquitination in Aged Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Plasmids, siRNAs and Transfection
4.3. Immunoblotting
4.4. Real-Time PCR
4.5. Co-Immunoprecipitation and Ubiquitination Assay
4.6. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
BAG2 | BCL2-associated athanogene 2 |
CFTR | Cystic fibrosis transmembrane conductance regulator |
CHIP | C-terminus of HSP70-interacting protein |
HSP | Heat shock protein |
HSPBP1 | HSP70 binding protein 1 |
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Schönbühler, B.; Schmitt, V.; Huesmann, H.; Kern, A.; Gamerdinger, M.; Behl, C. BAG2 Interferes with CHIP-Mediated Ubiquitination of HSP72. Int. J. Mol. Sci. 2017, 18, 69. https://doi.org/10.3390/ijms18010069
Schönbühler B, Schmitt V, Huesmann H, Kern A, Gamerdinger M, Behl C. BAG2 Interferes with CHIP-Mediated Ubiquitination of HSP72. International Journal of Molecular Sciences. 2017; 18(1):69. https://doi.org/10.3390/ijms18010069
Chicago/Turabian StyleSchönbühler, Bianca, Verena Schmitt, Heike Huesmann, Andreas Kern, Martin Gamerdinger, and Christian Behl. 2017. "BAG2 Interferes with CHIP-Mediated Ubiquitination of HSP72" International Journal of Molecular Sciences 18, no. 1: 69. https://doi.org/10.3390/ijms18010069
APA StyleSchönbühler, B., Schmitt, V., Huesmann, H., Kern, A., Gamerdinger, M., & Behl, C. (2017). BAG2 Interferes with CHIP-Mediated Ubiquitination of HSP72. International Journal of Molecular Sciences, 18(1), 69. https://doi.org/10.3390/ijms18010069