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Editorial to the Special Issue “Electrophysiology”
 
 
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Volume 22
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Correction to Int. J. Mol. Sci. 2020, 21(13), 4655.
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Correction

Correction: Feng et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int. J. Mol. Sci. 2020, 21, 4655

by
Duo Feng
,
DongZhu Xu
*,
Nobuyuki Murakoshi
,
Kazuko Tajiri
,
Rujie Qin
,
Saori Yonebayashi
,
Yuta Okabe
,
Siqi Li
,
Zixun Yuan
,
Kazutaka Aonuma
and
Masaki Ieda
Department of Cardiology, Institute of Clinical Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(19), 10881; https://doi.org/10.3390/ijms221910881
Submission received: 18 August 2021 / Accepted: 25 August 2021 / Published: 8 October 2021
(This article belongs to the Special Issue Electrophysiology)
Browse Figures
Versions Notes
The authors wish to make the following corrections to this paper [1]:
Changes in figures due to mislabeling. Figure 1E and Figure 4C were replaced with corrected figures. The picture changes will not affect the conclusion of the manuscript.
The authors would like to apologize for any inconvenience caused to the readers by these changes.

Reference

  1. Feng, D.; Xu, D.; Murakoshi, N.; Tajiri, K.; Qin, R.; Yonebayashi, S.; Okabe, Y.; Li, S.; Yuan, Z.; Aonuma, K.; et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int. J. Mol. Sci. 2020, 21, 4655. [Google Scholar] [CrossRef] [PubMed]
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Figure 1. Gene and protein expressions of Nampt and Sirt1 in the atrial tissues. (A) Nampt mRNA expression levels in the atrial tissues evaluated by quantitative PCR (n = 4 mice per each group). (B) Sirt1 mRNA expression levels in the atrial tissues evaluated by quantitative PCR (n = 4 mice per group). (C) Nmrk2 mRNA expression levels in the atrial tissues evaluated by quantitative PCR (n = 4 mice per group). (D) Representative Western Blots for Nampt and Sirt1 in the atrial tissues (n = 3 mice per group). (E) Nampt protein expression levels in the atrial tissues (n = 3 mice per group). (F) Sirt1 protein expression levels in the atrial tissues (n = 3 mice per group). (G) Representative images of Nampt immunohistochemistry in the atrial tissues. Nampt was mainly expressed in the cytoplasm of atrial cardiomyocytes. Scale bar: 50 µm. (H) Positively stained areas in the atrial tissues by immunohistochemistry. (I) Atrial NAD/NADH ratio in four studied groups (n = 4 mice per group). * p < 0.05 vs. WT+ND mice. Data are shown as mean ± SD. Statistical comparisons between multiple groups: one-way ANOVA followed by a post hoc Bonferroni test.
Figure 1. Gene and protein expressions of Nampt and Sirt1 in the atrial tissues. (A) Nampt mRNA expression levels in the atrial tissues evaluated by quantitative PCR (n = 4 mice per each group). (B) Sirt1 mRNA expression levels in the atrial tissues evaluated by quantitative PCR (n = 4 mice per group). (C) Nmrk2 mRNA expression levels in the atrial tissues evaluated by quantitative PCR (n = 4 mice per group). (D) Representative Western Blots for Nampt and Sirt1 in the atrial tissues (n = 3 mice per group). (E) Nampt protein expression levels in the atrial tissues (n = 3 mice per group). (F) Sirt1 protein expression levels in the atrial tissues (n = 3 mice per group). (G) Representative images of Nampt immunohistochemistry in the atrial tissues. Nampt was mainly expressed in the cytoplasm of atrial cardiomyocytes. Scale bar: 50 µm. (H) Positively stained areas in the atrial tissues by immunohistochemistry. (I) Atrial NAD/NADH ratio in four studied groups (n = 4 mice per group). * p < 0.05 vs. WT+ND mice. Data are shown as mean ± SD. Statistical comparisons between multiple groups: one-way ANOVA followed by a post hoc Bonferroni test.
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Figure 4. Calcium imaging of isolated cardiomyocytes. (A) Representative calcium images of isolated cardiomyocytes at baseline and after application of Iso obtained by confocal microscopy. (B) Representative images of 3D surface plot of calcium sparks of each groups. (C) Numbers of calcium sparks and mini waves per scan. Mini waves are defined as the calcium waves which did not transport throughout the cardiomyocyte, but only a portion of the cell. Base indicates baseline condition, and Iso indicates condition after adding isoproterenol (Iso). (D) Scatter chart of fractional fluorescence increases (F/F0). (E) Scatter chart of time to peak florescence signal. * p < 0.05 vs. WT+ND mice; † p < 0.05 vs. WT+ND+Iso mice; ‡ p < 0.05 vs. WT+HFD+Iso mice (n = 10 in WT+ND group, n = 7 in NKO+ND, n = 7 in WT+HFD group, n = 6 in NKO+HFD group). Statistical comparisons between multiple groups: one-way ANOVA followed by a post hoc Bonferroni test.
Figure 4. Calcium imaging of isolated cardiomyocytes. (A) Representative calcium images of isolated cardiomyocytes at baseline and after application of Iso obtained by confocal microscopy. (B) Representative images of 3D surface plot of calcium sparks of each groups. (C) Numbers of calcium sparks and mini waves per scan. Mini waves are defined as the calcium waves which did not transport throughout the cardiomyocyte, but only a portion of the cell. Base indicates baseline condition, and Iso indicates condition after adding isoproterenol (Iso). (D) Scatter chart of fractional fluorescence increases (F/F0). (E) Scatter chart of time to peak florescence signal. * p < 0.05 vs. WT+ND mice; † p < 0.05 vs. WT+ND+Iso mice; ‡ p < 0.05 vs. WT+HFD+Iso mice (n = 10 in WT+ND group, n = 7 in NKO+ND, n = 7 in WT+HFD group, n = 6 in NKO+HFD group). Statistical comparisons between multiple groups: one-way ANOVA followed by a post hoc Bonferroni test.
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MDPI and ACS Style

Feng, D.; Xu, D.; Murakoshi, N.; Tajiri, K.; Qin, R.; Yonebayashi, S.; Okabe, Y.; Li, S.; Yuan, Z.; Aonuma, K.; et al. Correction: Feng et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int. J. Mol. Sci. 2020, 21, 4655. Int. J. Mol. Sci. 2021, 22, 10881. https://doi.org/10.3390/ijms221910881

AMA Style

Feng D, Xu D, Murakoshi N, Tajiri K, Qin R, Yonebayashi S, Okabe Y, Li S, Yuan Z, Aonuma K, et al. Correction: Feng et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int. J. Mol. Sci. 2020, 21, 4655. International Journal of Molecular Sciences. 2021; 22(19):10881. https://doi.org/10.3390/ijms221910881

Chicago/Turabian Style

Feng, Duo, DongZhu Xu, Nobuyuki Murakoshi, Kazuko Tajiri, Rujie Qin, Saori Yonebayashi, Yuta Okabe, Siqi Li, Zixun Yuan, Kazutaka Aonuma, and et al. 2021. "Correction: Feng et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int. J. Mol. Sci. 2020, 21, 4655" International Journal of Molecular Sciences 22, no. 19: 10881. https://doi.org/10.3390/ijms221910881

APA Style

Feng, D., Xu, D., Murakoshi, N., Tajiri, K., Qin, R., Yonebayashi, S., Okabe, Y., Li, S., Yuan, Z., Aonuma, K., & Ieda, M. (2021). Correction: Feng et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int. J. Mol. Sci. 2020, 21, 4655. International Journal of Molecular Sciences, 22(19), 10881. https://doi.org/10.3390/ijms221910881

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