Role of UCP2 in the Energy Metabolism of the Cancer Cell Line A549
Abstract
:1. Introduction
2. Results
2.1. OXPHOS and Aerobic Glycolysis
2.2. Reactive Oxygen Species
2.3. Adenine Nucleotide Levels
2.4. Cell Growth
2.5. Chromane Derivatives
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Materials
4.2. Silencing
4.3. Western Blot Analysis
4.4. RTqPCR
4.5. Characterization of the Cellular Bioenergetics
4.6. Lactate Assay
4.7. Determination of Reactive Oxygen Species
4.8. Adenine Nucleotides Measurement
4.9. Cell Proliferation
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Segalés, J.; Sánchez-Martín, C.; Pujol-Morcillo, A.; Martín-Ruiz, M.; de los Santos, P.; Lobato-Alonso, D.; Oliver, E.; Rial, E. Role of UCP2 in the Energy Metabolism of the Cancer Cell Line A549. Int. J. Mol. Sci. 2023, 24, 8123. https://doi.org/10.3390/ijms24098123
Segalés J, Sánchez-Martín C, Pujol-Morcillo A, Martín-Ruiz M, de los Santos P, Lobato-Alonso D, Oliver E, Rial E. Role of UCP2 in the Energy Metabolism of the Cancer Cell Line A549. International Journal of Molecular Sciences. 2023; 24(9):8123. https://doi.org/10.3390/ijms24098123
Chicago/Turabian StyleSegalés, Jessica, Carlos Sánchez-Martín, Aleida Pujol-Morcillo, Marta Martín-Ruiz, Patricia de los Santos, Daniel Lobato-Alonso, Eduardo Oliver, and Eduardo Rial. 2023. "Role of UCP2 in the Energy Metabolism of the Cancer Cell Line A549" International Journal of Molecular Sciences 24, no. 9: 8123. https://doi.org/10.3390/ijms24098123
APA StyleSegalés, J., Sánchez-Martín, C., Pujol-Morcillo, A., Martín-Ruiz, M., de los Santos, P., Lobato-Alonso, D., Oliver, E., & Rial, E. (2023). Role of UCP2 in the Energy Metabolism of the Cancer Cell Line A549. International Journal of Molecular Sciences, 24(9), 8123. https://doi.org/10.3390/ijms24098123