Pseudomonas species are opportunistic pathogens with implications in a wide range of diseases including cystic fibrosis and sickle cell anaemia. Because of their status as multidrug resistant (MDR) and extremely drug resistant (XDR) bacteria
Pseudomonas species represent a threat to public health. Prevalence,
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Pseudomonas species are opportunistic pathogens with implications in a wide range of diseases including cystic fibrosis and sickle cell anaemia. Because of their status as multidrug resistant (MDR) and extremely drug resistant (XDR) bacteria
Pseudomonas species represent a threat to public health. Prevalence, antibiogram and associated antibiotic resistant genes of
Pseudomonas species isolated from freshwater and mixed liquor environments in the Eastern Cape Province of South Africa were assessed. Polymerase chain reaction (PCR) based technique was used to identify the isolates and screen for antibiotic resistant genes. The result shows occurrence of
Pseudomonas spp. in freshwater and mixed liquor as follows: 71.42% and 37.5% (
P. putida), 14.28% and 31.25% (
P. flourescens), 7.14% and 6.25% (
P. aeruginosa) and 7.14% and 25% for other
Pseudomonas species respectively. Disk diffusion antibiogram of the
Pseudomonas isolates from the two locations showed 100% resistance to penicillin, oxacillin, clindamycin, rifampicin and 100% susceptibility to ciprofloxacin and gentamicin with varied percentage resistances to cephalothin, nalidixic acid, tetracycline, and ampicillin. The
blaTEM antibiotic resistant gene was detected in 12.5% of
P. putida, 57.14% of
P. fluorescens, 100%
P. aeruginosa and 40% in other
Pseudomonas species. Similarly, Integrons conserved segment were detected in 12.5% of
P. putida, 57.14% of
P. fluorescens, 100% of
P. aeruginosa and 40% of other
Pseudomonas species. The presence of
blaTEM gene and integrons conserved segment in some of the isolates is worrisome and suggest
Pseudomonas species as important reservoirs of multidrug resistance genes in the Eastern Cape Province environment.
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