In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO
2 fixation. Cell growth under different CO
2 supply conditions (0.2, 0.4, 0.8, and 1.2 g
L
−1 d
−1), residual nitrate,
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In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO
2 fixation. Cell growth under different CO
2 supply conditions (0.2, 0.4, 0.8, and 1.2 g
L
−1 d
−1), residual nitrate, and pH value of
Chlorella sp. SJTU-3,
Chlorella pyrenoidosa SJTU-2, and
Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species (
Chlorella sp. SJTU-3,
Chlorella pyrenoidosa SJTU-2,
Selenastrum capricornutum,
Scenedesmus obliquus SJTU-3,
Chlamydomonas sajao,
Dunaliella primolecta) was carried out in flasks and the M96SS.
Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437 g L
−1) in anaerobic pond water with 0.8, and 1.2 g
L
−1 d
−1 CO
2. The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský’s system, and a microplate shaker.
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