Next Article in Journal
Serological Screening for Antibodies against SARS-CoV-2 in Dutch Shelter Cats
Next Article in Special Issue
Detection and Complete Genome Analysis of Circoviruses and Cycloviruses in the Small Indian Mongoose (Urva auropunctata): Identification of Novel Species
Previous Article in Journal
Exacerbation of Influenza A Virus Disease Severity by Respiratory Syncytial Virus Co-Infection in a Mouse Model
Previous Article in Special Issue
A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen® or Taqman® Technologies
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 13
Issue 8
10.3390/v13081632
Review Report
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

A New Molecular Detection System for Canine Distemper Virus Based on a Double-Check Strategy

Viruses 2021, 13(8), 1632; https://doi.org/10.3390/v13081632
by Sabrina Halecker 1,†, Sabine Bock 2, Martin Beer 1 and Bernd Hoffmann 1,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Viruses 2021, 13(8), 1632; https://doi.org/10.3390/v13081632
Submission received: 6 July 2021 / Revised: 9 August 2021 / Accepted: 11 August 2021 / Published: 18 August 2021
(This article belongs to the Special Issue Emerging Wildlife Viral Diseases)

Round 1

Reviewer 1 Report

This work developed novel methods for the safe detection of CDV in domestic and wild animals. The new methods achieved a sensitivity of 98.9% and a specificity of 100%, which is a reliable molecular diagnostic tool for the detection of CDV in domestic and wild animals. I have some suggestions shown below:

  1. In the results section, I highly recommend the authors to show a ROC curve to demonstrate the sensitivity and specificity. A comparison between the new method and previous methods should also be shown in the ROC curves.
  2. Further explanations are needed to reveal why the new tool can perform much better than previous methods.
  3. The sensitivity and specificity are extremely high. Besides such high performance, is there any limitation for the new tool?

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The authors efficiently address the problems generated in diagnostic systems by the genetic variability of CDV and the inconveniences generated by the multispecies potential of CDV. For this reviewer the paper presents an important methodological approach that could be really useful for too many veterinary diagnostic laboratories from different part of the world.

 

Minor changes

Author should include a paragraph addressing BIAS control in sampling and processing samples. Do the same lab operator perform all the analysis? , Were the Index new rt-qPCR results interpreted without knowledge of the results of the reference standard? , Was there an appropriate interval between index test(s) and reference standard?. For a comprehensive review, I recommend check the QUADAS guide that is designed to assess the quality of primary diagnostic accuracy studies (just as an example of the BIAS report).

 

Nice paper!

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

My questions have been addressed. I recommend it to be accepted.

Back to TopTop