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Review

Poxvirus Exploitation of the Ubiquitin-Proteasome System

by
Michele Barry
*,
Nicholas Van Buuren
,
Kristin Burles
,
Kelly Mottet
,
Qian Wang
and
Alastair Teale
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, T6G 2S2, Canada
*
Author to whom correspondence should be addressed.
Viruses 2010, 2(10), 2356-2380; https://doi.org/10.3390/v2102356
Submission received: 2 September 2010 / Revised: 27 September 2010 / Accepted: 30 September 2010 / Published: 19 October 2010
(This article belongs to the Special Issue Viruses and the Ubiquitin/Proteasome System)
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Abstract

:
Ubiquitination plays a critical role in many cellular processes. A growing number of viruses have evolved strategies to exploit the ubiquitin-proteasome system, including members of the Poxviridae family. Members of the poxvirus family have recently been shown to encode BTB/kelch and ankyrin/F-box proteins that interact with cullin-3 and cullin-1 based ubiquitin ligases, respectively. Multiple members of the poxvirus family also encode ubiquitin ligases with intrinsic activity. This review describes the numerous mechanisms that poxviruses employ to manipulate the ubiquitin-proteasome system.

1. Introduction

Ubiquitin is a 76 amino acid protein that is best known for its role in protein degradation [1], however, the addition of ubiquitin can also serve roles not associated with protein degradation [2]. The post-translational addition of ubiquitin onto proteins occurs through a three step enzymatic cascade [3]. Ubiquitin is initially activated by one of two ubiquitin activating enzymes. Activated ubiquitin is subsequently transferred to a ubiquitin conjugating enzyme. In the final step, a ubiquitin ligase is responsible for the transfer of ubiquitin to the target protein. Proteins can be modified by mono-ubiquitin or poly-ubiquitin [4]. Any one of seven lysine residues present in ubiquitin allows for the formation of ubiquitin chains. Lysine 48 and 63 are the most commonly used. Polyubiquitin chains formed on lysine 48 typically results in degradation through the 26S proteasome [1]. Conversely, polyubiquitin chains formed on lysine 63 tend to alter protein function [2]. More recently, linear ubiquitin has been associated with the regulation of nuclear factor κB, and the ubiquitination of non-lysine residues has also been described [5,6].
The Poxviridae are a large family of viruses that infect a wide range of vertebrates and invertebrates [7]. The best known member of the family is variola virus, the causative agent of smallpox. Global eradication of smallpox was achieved in 1979 through a vaccination program initiated by the World Health Organization (WHO) [8]. Smallpox eradication used vaccinia virus, a close relative of variola virus, as a live vaccine [9]. By virus standards, poxvirus genomes are large ranging in size from 150–300 kbp; encoding upwards of 200 or more open reading frames [7]. Much interest in poxvirus biology stems from the observation that poxviruses employ a vast array of effective immune evasion strategies [10,11]. Additionally, the ease with which recombinant poxviruses are generated has made them attractive viruses for dissecting cellular signaling pathways [10]. Recently, protein ubiquitination has emerged as an important mechanism for the control of protein degradation and function, especially during virus infection [12,13,14,15,16]. In this review we focus on the strategies that poxviruses have developed to exploit the ubiquitin-proteasome system.

2. Poxvirus Encoded Ubiquitin

Ubiquitination is a post-translational modification that plays an essential role in many cellular processes [17]. Ubiquitin is a small 76 amino acid protein that is highly conserved in eukaryotes. In fact, only four amino acids differ between yeast, plants and mammalian ubiquitin sequences [18]. Two classes of ubiquitin-encoding genes are present in eukaryotic genomes. These including polyubiquitin genes that encode back-to-back ubiquitin sequences that are cleaved to produce ubiquitin monomers, and ubiquitin-carboxyl extension protein (CEP) fusion genes that encode a single ubiquitin sequence fused to a ribosomal sequence at the C-terminus that is incorporated into the ribosome [19]. Ubiquitin contains seven lysine residues that can be used to build ubiquitin chains [1,17,20]. Traditionally, ubiquitination is associated with protein degradation; however, current evidence indicates that ubiquitination has additional regulatory functions [1,17,20].
Ubiquitin homologs have recently been identified in the genomes of poxviruses [21,22,23]. Two insect poxviruses: Melanoplus sanguinipes (MSEV) (Table 1) [21] and Amsacta moorei (AMEV) [22], as well as canarypox virus (CNPV), contain virus-encoded ubiquitin homologs [23]. Genomic analysis of MSEV, a poxvirus that infects locusts, identified the open reading frame MSEV144; encoding an 80 amino acid protein that is 86% identical to human ubiquitin (Figure 1) [21]. However, the role that MSEV144 plays during viral infection has not been characterized. BLAST analysis also identified an additional poxvirus-encoded ubiquitin gene AMEV180, in AMEV, a poxvirus that infects moths. AMEV180 is 81 amino acids in length and 89% identical to human ubiquitin (Figure 1) [22]. Sequencing of canarypox, a poxvirus that infects song birds, identified another ubiquitin homolog, CNPV096, in canarypox virus (Figure 1) [23]. At 85 amino acids in length, CNPV096 contains all of the residues required for protein ubiquitination and is 98% identical to human ubiquitin [23]. Interestingly, fowlpox virus (FWPV), a close relative of canarypox virus, contains fragmented remains of a functional ubiquitin gene [24]. MSEV144, CNPV096, and AMEV180 are not part of the polyubiquitin gene class, and do not encode ribosomal peptides at the C-termini. In contrast to eukaryotes, which have multiple copies of ubiquitin-encoding genes, only one copy of each ubiquitin gene is present in the genomes of these viruses [21,22,23]. Virus-encoded ubiquitin genes have also been identified in Baculoviridae, a family of dsDNA viruses that infect insects [25]. Disruption of the ubiquitin gene in Autographa californica nuclear polyhedrosis virus (AcNPV) has no effect on virus viability, however, a decrease in virion budding and total infectious particles was observed [25]. Whether the ubiquitin-encoding genes in MSEV, AMEV, and CNPV are required for productive infection or virion budding remains to be determined.
Although most poxviruses do not encode their own ubiquitin genes, ubiquitin is associated with the virion. Proteomic analysis of vaccinia virus indicates that ubiquitin accounts for approximately 3% of total virion protein [29]. Additionally, a lipid-modified form of ubiquitin is associated with several viruses [25,30,31]. For example, baculovirus, African swine fever virus, herpes simplex virus and vaccinia virus incorporate lipid-modified ubiquitin in their envelopes [25,29,30,31]. Previous analysis of baculovirus AcNPV demonstrated that lipid-modified ubiquitin was present and that ubiquitin was host derived [25]. Scavenging ubiquitin from the host may represent another strategy used by poxviruses to increase the levels of ubiquitin available during infection. Alternatively, lipid-modified ubiquitin may exist in cell membranes for a cellular function, such as autophagosome formation, and the virus simply acquires it passively during envelope acquisition. Whether other poxviruses have lipid-modified ubiquitin incorporated into their envelopes has not been studied.
It seems unlikely that poxviruses would maintain an open reading frame that has no role during infection. To date, the function of the poxvirus encoded ubiquitin sequences has not been determined. Encoding additional pools of ubiquitin could be a mechanism used by entomopoxiruses and canarypox virus to increase efficiency of host cell modulation during infection. It is also possible that these viruses rely heavily on the ubiquitin-proteasome system. For example, it has been shown that members of the Orthopoxvirus family require a functional ubiquitin-proteasome system for productive infection [32,33]. Alternatively, viral-encoded ubiquitin homologs may function to inhibit the ubiquitin proteasome system. The AcNPV-encoded ubiquitin functions as a chain terminator for K48 linked polyubiquitination, the linkage that targets proteins for degradation by the 26S proteasome [34]. As such, it is possible that poxvirus-encoded ubiquitin may also act as chain terminators to inhibit degradation of certain substrates. At present, the reason that only a few members of the poxvirus family encode ubiquitin homologs remains unclear.

3. Poxvirus Encoded Ubiquitin Ligases

Poxviruses encode two families of proteins with intrinsic ubiquitin ligase activity; a membrane-associated RING-CH (MARCH) ubiquitin ligase, and a really interesting new gene (RING) finger protein (Figure 2A and B) [35,36,37,38].
The MARCH family of proteins contains a modified RING domain (RING-CH) at the N-terminus as well as transmembrane domains that promote localization to membranes (Figure 2A) [39]. Cellular MARCH proteins play an important role in the down-regulation of membrane receptors including MHC class I, MHC class II, and CD4 [39]. In addition to a family of cellular MARCH proteins, MARCH ubiquitin ligases also exist in the genomes of herpesviruses and poxviruses (Table 1) [40]. Infection with myxoma virus (MYXV), a rabbit specific poxvirus that causes myxomatosis, results in reduction of cell surface MHC class I [35,41,42,43]. The loss of MHC class I upon myxoma virus infection was later associated with ubiquitin ligase activity of the myxoma virus encoded MARCH homolog, M153R [35,41]. In addition to the down-regulation of MHC class I, M153R also reduces cell surface expression of CD95, ALCAM (CD66) and CD4 [35,37,41]. The loss of CD4 by M153R has been well characterized. Upon infection, M153R ubiquitinates the cytoplasmic tail of CD4, leading to its internalization via endocytosis and subsequent lysosomal degradation [37]. Through the action of M153R, myxoma virus induced ubiquitination and degradation of cell surface immune molecules provides an important mechanism for dampening the immune response.
p28 is a virus-encoded RING finger ubiquitin ligase that plays an important role in virulence (Figure 2B) [36,44,45]. p28 is highly conserved among pathogenic poxviruses and is expressed at both early and late times during virus infection (Table 1) [44,45]. The p28 ubiquitin ligase contains two functional domains; an N-terminal DNA binding domain, and a C-terminal RING domain (Figure 2B). The DNA binding domain of p28, referred to as KilA-N, remains largely uncharacterized [46]. This domain is found in a number of large DNA viruses as well as bacteria and bacteriophage [46]. The KilA-N domain plays an important role in the localization of p28 cytoplasmic viral factories [47,48]. In addition to being found in combination with a RING domain, the KilA-N domain is also found independently in some poxviruses. For example, eight KilA-N proteins are encoded in fowlpox virus (FWPV) and 23 KilA-N proteins are encoded in canarypox virus [23]. However, only two proteins in fowlpox virus and canarypox virus combine a KilA-N domain with a RING domain, likely encoding functional ubiquitin ligases [23,24,46].
The C-terminal RING domain of p28 is responsible for ubiquitin ligase activity [36,38]. p28 displays sequence homology to a family of cellular proteins termed Makorin (MKRN), however, this homology is restricted to the RING domain [49]. It has been suggested that the p28 family of poxvirus proteins were acquired through a fusion event of an existing KilA-N domain and a cellular MKRN [49]. Point mutations in the critical conserved residues of the RING domain disrupt ubiquitination [36,38]. Using in vitro ubiquitination assays, p28 homologs in ectromelia virus (ECTV), vaccinia virus (VV)-strain IHDW and variola virus (VARV) were shown to function as ubiquitin ligases [36,38]. The p28 ortholog in variola virus, D4R, functions in vitro with the ubiquitin conjugating enzymes, Ubc4 and UbcH5c [36]. Work in our laboratory has demonstrated that expression of p28 targets conjugated ubiquitin to viral factories [38]. K48 linked ubiquitin, which is associated with protein degradation, also co-localized at the virus factory with p28 [48]. Given that K48 linked ubiquitin is associated with proteasomal degradation it is likely that p28 plays a role in targeting substrates for degradation. Interestingly, variola virus D4R functions in vitro with Ubc13, the only known ubiquitin conjugating enzyme that promotes K63 linkages [36]. In contrast to K48 linkages, K63 linkages are associated with non-proteolytic functions, suggesting that p28 may form K63 linkages during virus infection [36]. To date, no p28 substrates have been identified. However, p28 has been implicated in the inhibition of apoptosis [47,50]. It is therefore tempting to speculate that p28 may be targeting pro-apoptotic proteins for degradation. Since p28 localizes to viral factories, it is likely that potential substrates are located at the viral factory. Additionally, since p28 is expressed early during infection, prior to virus factory formation, p28 may also be responsible for ubiquitinating cytoplasmic substrates.
In vivo studies have shown that p28 is a critical virulence factor during ectromelia virus (ECTV) infection [44]. In susceptible strains of mice, ectromelia virus devoid of p28 was extremely attenuated and all mice recovered; this is in sharp contrast to mice infected with wild-type virus, which succumb to infection [44]. Both wild type ectromelia virus and ectromelia virus devoid of p28 replicated equally well in all cell lines tested except for primary peritoneal macrophages [44,45]. Macrophages are thought to be critical for the transport of the virus, suggesting that the ubiquitin ligase activity of p28 plays an important role in peritoneal marcrophages [45]. The role of p28 in virulence and its ability to function as a bona fide ubiquitin ligase suggests p28 is ubiquitinating substrates, however these substrates have yet to be identified. Identification of p28 substrates will undoubtedly provide important clues into the role of p28 in virus virulence.

4. A Family of Poxvirus Encoded BTB/Kelch Proteins

The BTB domain, also known as the POZ, Bric-a-Brac, Tramtrack, or Broad-complex, is a highly conserved protein-protein interaction motif that is involved in many cellular functions, including transcriptional and cytoskeletal regulation [51,52,53]. Recently, cellular BTB domain-containing proteins have been shown to function as substrate-specific adaptors of cullin-3 based ubiquitin ligase to target proteins for ubiquitination [54,55,56,57]. Unlike the well-characterized SCF (Skp1/Cul1/F-box) and ECS (elonginC/Cul2/SOCS) E3 complexes, in which Skp1/F-box or elonginC/SOCS combine to bridge substrates to cullins, BTB proteins fulfill this function through a single polypeptide containing the BTB domain as a linker to cullin-3 and a substrate-recruiting domain, such as kelch, MATH or Zinc Fingers (Figure 2C) [54,55,56,57]. Supporting this, the Skp1 and elonginC proteins display similar three-dimensional structure as the BTB domain [57,58,59]. The kelch domain consists of multiple repeated kelch motifs, and is thought to mediate protein-protein interactions (Figure 2C) [60].
A large group of BTB/kelch proteins have been identified in most members of the poxvirus family (Table 1) [61]. For example, vaccinia virus encodes three BTB/kelch proteins [62]; cowpox virus (CPXV) encodes six BTB/kelch proteins [63]; ectromelia virus strain Moscow (EVM) encodes four such proteins [64]; while monkeypox virus (MPXV) encodes only one BTB/kelch gene [65] (Table 1). Although the specific roles of the poxvirus BTB/kelch proteins are still unclear, it has been speculated that they may function as cullin-3 substrate-specific adaptors, similar to their cellular counterparts. In agreement with this idea, the BTB domains of ectromelia virus encoded BTB/kelch proteins EVM150 and EVM167 are essential and sufficient for interaction with cullin-3 [66]. Consistently, EVM150 and EVM167 associate with conjugated ubiquitin and Roc1, the RING-finger protein required for an active cullin-3 ubiquitin ligase complex [66]. The other two ectromelia virus encoded BTB/kelch proteins, EVM018 and EVM027, also interact with cullin-3 [67]. Interestingly, EVM004, an ectromelia virus encoded protein containing only a BTB domain, does not interact with cullin-3, Roc1, or conjugated ubiquitin, suggesting that, unlike the other ectromelia virus encoded BTB/kelch proteins, EVM004 may function independently of the ubiquitin-proteasome pathway [67]. The failure of EVM004 to interact with cullin-3 is currently unknown. Together, these findings suggest that poxviruses may employ BTB/kelch-cullin-3 ubiquitin ligase complex as another strategy to manipulate the cellular environment. Alternatively, the poxvirus BTB/kelch proteins may function by simply sequestering cullin-3 to inhibit the cullin-3-based cellular ubiquitin pathway. Given that poxviruses encode multiple BTB/kelch proteins with different kelch regions, it is probable that these viral BTB/kelch proteins function to specifically target different substrates to the cullin-3 ubiquitin ligase for ubiquitination.
The importance of the poxvirus BTB/kelch proteins during virus infection has been studied. Vaccinia virus devoid of the BTB/kelch proteins C2L, F3L or A55R, the orthologs of EVM018, EVM027 and EVM150, respectively, displays an altered viral pathogenesis in the murine intradermal model [68,69,70]. Deletion of four BTB/kelch genes, D11L, C18L, G3L and A57R, from cowpox virus strain GRI-90 also results in altered host range and attenuated virulence [71]. Additionally, sheeppox virus (SPPV) BTB/kelch gene SPPV-019 has been shown to modulate cellular adhesion and affect virus virulence using a SPPV-019 knock-out virus model [72]. These observations suggest that BTB/kelch proteins function to manipulate the cellular host environment. To date, however, no definite substrates for the poxvirus BTB/kelch proteins have been identified, although several targets for cellular BTB/kelch proteins have been characterized. For example, NRF2, a critical nuclear transcription factor regulating oxidative stress, is degraded by KEAP1/cullin-3 ubiquitin ligase [73,74]. KEAP1 also functions as an IKKβ ubiquitin ligase [75]. Aurora B, a chromosomal passenger protein responsible for the proper progression of mitosis and cytokinesis, is targeted by KLHL21/Cul3 E3 for ubiquitination [76]. Interestingly, the vaccina virus encoded BTB/kelch protein, WR026 (COP-C2L), was recently shown through yeast-2-hybrid screening to interact with cellular crystallin alpha B (CRYAB), a small heat-shock protein [77]. Whether crystalline alpha B can be regulated by WR026 for cullin-3-mediated ubiquitination needs to be investigated. Although the role of poxvirus BTB/kelch proteins is still undefined, many other viruses have evolved mechanisms to specifically recruit cellular proteins to cullin-based ubiquitin ligases [12,14]. Future identification of the substrates targeted by the poxvirus BTB/kelch proteins will provide new insight into the understanding of cellular anti-viral responses.

5. Poxvirus Encoded Ankyrin/PRANC Proteins

Ankyrin repeat proteins represent one of the largest families of proteins encoded by poxviruses. The ankyrin repeat consists of a 33 amino acid helix-loop-helix motif with a highly conserved amino acid sequence [78,79,80]. Ankyrin repeats were first identified in the cytoskeletal structural protein called ankyrin, which contains 24 ankyrin repeats [81]. Since its discovery, the ankyrin repeat has been characterized in a wide variety of cellular proteins, and generally mediates unique protein-protein interactions [78,79,80]. With the exception of molluscipoxviruses, all other poxvirus families encode a large repertoire of ankyrin proteins (Table 1). The largest family is encoded by canarypox virus and is comprised of 51 ankyrin repeat proteins, representing 21% of the canarypox virus genome [23,82]. Poxviral ankyrin repeat proteins are large proteins, ranging from 400–650 amino acids in length, containing between 5 to 10 ankyrin repeats located at their N-termini. Although the poxviral ankyrin repeat proteins contain no obvious structural domains at their C-termini, many of the proteins display a conserved sequence, which upon closer inspection was shown to resemble the F-box domain that functions in the recruitment of substrates to the cellular SCF (Skp-1, cullin, F-box) ubiquitin ligase complex [82,83,84]. The poxviral F-box-like domain was later named PRANC (pox protein repeat of ankyrin C-terminus) (pfam.janelia.org/family/PF09372). The SCF complex is a highly conserved ubiquitin ligase involved in regulation of the cell cycle, DNA repair, and innate immunity [17,85,86]. The complex consists of cullin-1, which serves as the molecular scaffold, Roc1, a RING finger ubiquitin ligase, Skp1, the linker protein, and one of over 70 known cellular F-box proteins which function in substrate recruitment (Figure 2D) [84,85,86]. Cellular F-box proteins consist of N-terminal F-box domains in conjunction with C-terminal protein binding domains such as WD40 repeats or leucine-rich repeats (LRR) [84,85,86]. The F-box domain consists of a highly conserved 50 amino acid sequence, folding into three alpha-helices, which function to bind the linker protein, Skp1, while WD40 repeats or LRRs function to bind substrates which are subsequently ubiquitinated through the ubiquitin ligase activity of Roc1 (Figure 2D) [59]. Substrates of the SCF ubiquitin ligase complex typically require a phosphorylation event prior to recognition by the substrate adaptor [83,84,85,86].
Until the recent identification of Ank/PRANC proteins in the parasitoid wasp, Nasonia, Ank/PRANC proteins were thought to be unique to poxviruses [87]. The poxvirus Ank/PRANC proteins differ from cellular F-box proteins in two important aspects. Firstly, the C-terminal location of the poxvirus F-box-like domain is unique to this set of proteins, and secondly, the poxvirus Ank/PRANC proteins contain truncated F-boxes (Figure 3) [82,88,89]. The cowpox virus encoded Ank/PRANC protein CP77 contains a PRANC domain that is only 13 amino acids in length and may represent the minimum requirement for interaction with Skp1 [90]. A related family of proteins, the suppressor of cytokine signaling (SOCS)-box family appear in conjunction with ankyrin repeats, and function as substrate adaptor molecules for the ECS ubiquitin ligases [91]. Since the SOCS-box and the F-box share sequence similarity it has been proposed that the poxviral ankyrin/F-box proteins were acquired as SOCS-box proteins that have evolved to regulate the cullin-1 based ligase [82,92]. In addition to poxvirus encoded Ank/PRANC proteins, poxviruses also encode ankyrin only proteins [82,93,94]. These ankyrin-only proteins do not contain PRANC domains, and have been proposed to have arisen from full length Ank/PRANC proteins [82].
Ank/PRANC proteins have been identified in a wide range of poxviruses including vaccina virus, ectromelia virus, cowpox virus and Orf virus. Studies on myxoma virus identified the first interaction between a poxviral Ank/PRANC protein, M-T5, and the SCF complex [95]. MT-5, one of four Ank/PRANC proteins in myxoma virus, co-localizes with cullin-1 in the nucleus and regulates the cell cycle and interacts with Akt [95,96]. Myxoma virus encodes four Ank/ PRANC proteins (M-T5, M148, M149, M150) all of which play a role in myxoma virus virulence [97,98,99]. Interestingly, M150 co-localized to the nucleus with the p65 subunit of nuclear factor kappa B (NF-κB), suggesting that M150 is involved in inhibition of NF-κB [99]. Each of the five Orf virus encoded Ank/PRANC proteins have been shown to associate with a functional SCF ubiquitin ligase complex, as demonstrated through in vitro ubiquitination assays [92]. In the case of the Orf virus proteins, the F-box-like domain was both necessary and sufficient to mediate the interaction with Skp1 and cullin-1 [92]. Similarly, the F-box domains of proteins from ectromelia virus, cowpox virus, and vaccinia virus are also essential for interaction with the SCF complex [89,90,100]. The cowpox virus encoded Ank/PRANC protein, CP77, functions as a host range protein that interacts with the NF-κB transcription factor, p65, to inhibit the transcription of inflammatory cytokines [90,101]. Regulation of the NF-κB signaling pathway by poxviral Ank/PRANC proteins appears to be a common trend. Using a yeast two-hybrid screen, the variola virus encoded G1R Ank/PRANC protein was shown to interact with the NF-κB regulatory protein NFκB1/p105 as well as Skp1 [102]. G1R, and its orthologs in cowpox virus, monkeypox virus, and ectromelia virus (CPXV006, MPXV003, EVM002), bind p105, and inhibit G1R degradation following TNFα stimulus [102]. Additionally, a CPXV006 deletion virus displayed increased release of proinflammatory cytokines in culture, and was slightly attenuated in C57BL/6 mice infected [103].
Although substrates have not been identified for the poxviral Ank/PRANC proteins, it has been hypothesized that the poxvirus Ank/PRANC proteins function as substrate adaptor proteins for the SCF complex. Although it is possible that the poxvirus Ank/PRANC proteins may simply bind and inhibit the SCF complex, this seems unlikely due to the large number of unique Ank/PRANC proteins encoded by poxviruses. For example, fowlpox virus encodes 20 Ank/PRANC proteins, each potentially targeting unique protein(s) for ubiquitination by the SCF complex (Table 1) [24]. Additionally, the ectromelia virus and Orf virus Ank/PRANC proteins have both been shown to associate with functional SCF complexes, suggesting that these proteins do not simply function as inhibitors [89,92]. The identification of substrates recruited to the SCF complex by poxviral Ank/PRANC proteins will be an essential step towards understanding this interesting family.

6. Regulation of the APC/C by Poxviruses

The anaphase promoting complex/cyclosome (APC/C) is the largest known cellular ubiquitin ligase complex, composed of at least 12 subunits (Figure 2E) [104]. Since its discovery almost 15 years ago its structure and regulation have proven to be increasingly complex. It is thought that the APC/C complex has evolved from an ancestral SCF-type ubiquitin ligase since the subunits APC2 and APC11 resemble a cullin-family member and RING-type E3 ligase, respectively. APC2 functions as the molecular scaffold, and contains cullin homology and binds to the RING-finger protein APC11 [105]. APC11 has been shown to recruit ubiquitin-conjugating enzymes to the APC/C in order to catalyze the in vitro transfer of ubiquitin onto target substrates [106]. Substrates for the APC/C are recognized through the presence of D-box or KEN-box domains, which are recognized by a variety of APC/C components including Cdh1, Cdc20 and Doc1 [107,108]. The APC/C plays a major role in regulation of the cell cycle at several points, and most well known for its ability to degrade securin, a protein that regulates the separation of sister chromatids during anaphase [109].
A family of poxvirus RING-finger proteins was recently identified that contain sequence similarity with the RING domain of the APC/C subunit APC11 (Table 1) [110]. These APC11 homologs were identified in the parapoxviruses, molluscipoxviruses, as well as the crocodilepox and squirrelpox viruses. The poxvirus encoded APC11 homolog from Orf virus, a member of the parapoxvirus family, is the only homolog studied to date and has been named PACR (poxvirus APC/cyclosome regulator) [110]. PACR was shown to co-precipitate with APC/C subunits APC2, APC3 and APC4, and is shown to associate with the APC/C complex in a similar manner to APC11 [110]. However, upon sequence analysis, PACR and the other poxvirus orthologs contain mutations within the RING domain that inhibit the binding of E2 ubiquitin-conjugating enzymes to the complex, and therefore inhibit substrate ubiquitination [110]. It is thought that inhibition of APC/C may prompt cells into S-phase, a stage within the cell cycle where additional cellular factors may be present and contribute to virus replication. Additionally, two of the targets of the APC/C are cellular ribonucleotide reductase and thymidine kinase proteins, proteins that contribute to the free nucleotide pools required for DNA synthesis. Typically poxviruses encode their own thymidine kinase and ribonucleotide reductase genes, however, the viral thymidine kinase and ribonucleotide reductases genes are absent from Orf virus as well as other virus that encode homologs of PARC. In contrast, many viruses that encode their own thymidine kinase genes, lack PACR orthologs. It has been hypothesized that one of the main reasons for encoding APC/C inhibitors is to upregulate cellular thymidine kinase and ribonucleotide reductase genes to enhance free nucleotide pools in poxviruses that lack the ability to promote this themselves.

7. Role of the Ubiquitin-Proteasome System During Poxvirus Infection

Poxviruses are renowned for creating an optimal environment for viral replication and propagation [7,11,111]. The ubiquitin-proteasome system, which plays a crucial role in protein degradation and cellular homoeostasis, is an attractive target for virus-encoded effector proteins. The ubiquitin-proteasome system is involved in regulating many important host pathways including antigen presentation, cell cycle progression, signal transduction, and DNA repair [1,17]. Individual interactions between poxviral proteins and the ubiquitin-proteasome system have been characterized [15,16]. The study of the ubiquitin-proteasome system has been aided greatly by the use of chemical proteasome inhibitors. These inhibitors block the catalytic action of the proteasome by preventing the degradation of ubiquitinated proteins and reducing the amount of free ubiquitin available within the cell [112]. Proteasome inhibitors, including MG132, act to reversibly inhibit proteasome action while others, including MG115, lactacystin, and epoxomycin irreversibly inhibit the proteasome [113,114]. Notably, the bortezomib, sold under the trade name Velcade®, and licensed for the treatment of multiple myeloma, is a potent inhibitor of the proteasome [115]. The overall importance of a functioning ubiquitin-proteasome system during poxvirus infection has only recently been investigated [32,33].
It has now been demonstrated that a functioning ubiquitin-proteasome system is vital to a successful infection by members of the Orthopoxvirus family [32,33]. In the presence of proteasome inhibitors, poxvirus replication is dramatically impaired [32,33]. Early poxviral gene expression is unaffected while intermediate and late gene expression is greatly reduced through the action of chemically distinct proteasome inhibitors. Viral factories, which normally appear as DNA rich areas in the cytoplasm of infected cells, are unable to form in the presence of proteasome inhibitors. In addition, it has been shown that plasmid replication, which can normally occur during poxvirus replication at viral factories [116], is blocked by the use of proteasome inhibitors [33]. The addition of proteasome inhibitors post-infection indicates that the block affects an early step during poxviral infection but does not affect the entry of poxvirus particles into the cell. Intriguingly, inhibition of the ubiquitin activating enzyme results in a similar phenotype during infection. Since overexpression of ubiquitin is unable to rescue late protein expression, DNA production and the generation of progeny virus, this data suggests that a functional ubiquitin-proteasome system as a whole is required for successful poxvirus infection [33]. Together, these observations indicate that viral DNA replication does not occur upon proteasome inhibition. The lack of viral DNA replication along with the pattern of gene expression seen upon treatement with proteasome inhibitors, points to viral uncoating and DNA replication as the likely candidates for the stage in the poxviral lifecycle actively blocked by proteasome inhibitors [32,33]. Further studies will undoubtedly lead to a greater understanding of the interactions between poxviruses and the ubiquitin-proteasome system and specifically the role of the proteasome during infection.
The dramatic effect of proteasome inhibitors on poxvirus infection, suggests the proteasome may be an attractive target for the development of antivirals. Interestingly, proteasome inhibitors demonstrate an antiviral effect on a wide range of viruses including human immunodeficiency virus [117], influenza virus [118], vesicular stomatitis virus [118], coronavirus [119], human cytomegalovirus [120], respiratory syncytial virus [121], herpes simplex virus [122] and hepatitis B virus [123]. As such, proteasome inhibitors seem to demonstrate antiviral activity though distinct mechanisms among viral species. For example, proteasome inhibitors have been shown to impair entry and RNA synthesis during coronavirus infection [119], inhibit the entry of herpes simplex virus into the nucleus [122], and inhibit influenza and vesicular stomatitis virus replication [118]. However, in vivo studies recently conducted have produced mixed results. Treatment with bortezomib results in a decrease of circulating RNA in mice chronically infected with Hepatitis B [123], but proteasome inhibition enhances the disease and mortality in mouse hepatitis coronavirus [119], as well, increasing inflammation and mortality was observed in human respiratory syncytial virus [121]. A possible explanation for the seemingly conflicting results between the in vitro and in vivo experiments is through modulation of the immune system by proteasome inhibitors. It has been demonstrated that proteasome inhibitors affect antigen processing in vivo [124]. While proteasome inhibition may be antiviral, the effects on the immune system caused by proteasome inhibitors may increase susceptibility and mortality in some viral infections. Still, the proteasome remains a possible target for antiviral development against poxviruses and it would be interesting to determine whether proteasome inhibitors are able to inhibit poxvirus disease and mortality in vivo.

8. Conclusions

Since the first realization that poxviruses encode proteins with intrinsic ubiquitin ligase activity, the field has grown at a fast and exciting pace. It is clear from the current research that poxviruses encode multiple proteins that manipulate the ubiquitin-proteasome system. As discussed here, these strategies include the expression of poxvirus-encoded ubiquitin, ubiquitin ligases, BTB/kelch proteins, Ank/PRANC proteins, as well as inhibitors of the APC/C complex. The presence of multiple poxvirus-encoded proteins suggests that poxviruses exploit the ubiquitin-proteasome in order to regulate cellular processes. In support of this, our recent observations indicate that upon infection with vaccinia virus the ubiquitin proteasome system is fully functional [125]. Within the field we have good track record of identifying and characterizing the poxvirus proteins involved in the ubiquitin-proteasome system. However, to date few substrates have been identified. Future studies are likely to focus on the identification of substrates for these viral ubiquitin ligases, as recent advancements in proteomics and mass spectrometry have paved the way to identifying ubiquitinated proteins [126,127,128,129]. Future studies will further our understanding of the intricate relationship between poxvirus replication and the ubiquitin-proteasome system.

References and Notes

  1. Pickart, C.M.; Fushman, D. Polyubiquitin chains: Polymeric protein signals. Curr. Opin. Chem. Biol. 2004, 8, 610–616. [Google Scholar] [CrossRef]
  2. Chen, Z.J.; Sun, L.J. Nonproteolytic functions of ubiquitin in cell signaling. Mol. Cell 2009, 33, 275–286. [Google Scholar] [CrossRef] [PubMed]
  3. Giles, J. Chemistry Nobel for trio who revealed molecular death-tag. Nature 2004, 431, 729. [Google Scholar] [CrossRef] [PubMed]
  4. Komander, D. The emerging complexity of protein ubiquitination. Biochem. Soc. Trans. 2009, 37, 937–953. [Google Scholar] [CrossRef] [PubMed]
  5. Cadwell, K.; Coscoy, L. Ubiquitination on nonlysine residues by a viral E3 ubiquitin ligase. Science 2005, 309, 127–130. [Google Scholar] [CrossRef]
  6. Iwai, K.; Tokunaga, F. Linear polyubiquitination: A new regulator of NF-kappaB activation. EMBO Rep. 2009, 10, 706–713. [Google Scholar] [CrossRef]
  7. Moss, B. Poxviridae: The viruses and their replication. In Fields Virology; Fields, B.N., Knipe, D.M., Howley, P.M., Eds.; Lippincott-Raven Publishers: Philadelphia, PA, USA, 1996; pp. 2637–2671. [Google Scholar]
  8. Wehrle, P.F. A reality in our time—Certification of the global eradication of smallpox. J. Infect. Dis. 1980, 142, 636–638. [Google Scholar] [CrossRef]
  9. Jacobs, B.L.; Langland, J.O.; Kibler, K.V.; Denzler, K.L.; White, S.D.; Holechek, S.A.; Wong, S.; Huynh, T.; Baskin, C.R. Vaccinia virus vaccines: Past, present and future. Antivir. Res. 2009, 84, 1–13. [Google Scholar] [CrossRef]
  10. Johnston, J.B.; McFadden, G. Technical knockout: Understanding poxvirus pathogenesis by selectively deleting viral immunomodulatory genes. Cell. Microbiol. 2004, 6, 695–705. [Google Scholar] [CrossRef]
  11. Seet, B.T.; Johnston, J.B.; Brunetti, C.R.; Barrett, J.W.; Everett, H.; Cameron, C.; Sypula, J.; Nazarian, S.H.; Lucas, A.; McFadden, G. Poxviruses and immune evasion. Annu. Rev. Immunol. 2003, 21, 377–423. [Google Scholar] [CrossRef]
  12. Barry, M.; Fruh, K. Viral modulators of cullin RING ubiquitin ligases: Culling the host defense. Sci. STKE 2006, 2006, pe21. [Google Scholar] [CrossRef]
  13. Isaacson, M.K.; Ploegh, H.L. Ubiquitination, ubiquitin-like modifiers, and deubiquitination in viral infection. Cell Host Microbe 2009, 5, 559–570. [Google Scholar] [CrossRef] [PubMed]
  14. Randow, F.; Lehner, P.J. Viral avoidance and exploitation of the ubiquitin system. Nat. Cell Biol. 2009, 11, 527–534. [Google Scholar] [CrossRef]
  15. Shchelkunov, S.N. Interaction of orthopoxviruses with the cellular ubiquitin-ligase system. Virus Genes 2010. [Google Scholar] [CrossRef]
  16. Zhang, L.; Villa, N.Y.; McFadden, G. Interplay between poxviruses and the cellular ubiquitin/ubiquitin-like pathways. FEBS Lett. 2009, 583, 607–614. [Google Scholar] [CrossRef]
  17. Weissman, A.M. Themes and variations on ubiquitylation. Nat. Rev. Mol. Cell. Biol. 2001, 2, 169–178. [Google Scholar] [CrossRef]
  18. Glickman, M.H.; Ciechanover, A. The ubiquitin-proteasome proteolytic pathway: Destruction for the sake of construction. Physiol. Rev. 2002, 82, 373–428. [Google Scholar] [CrossRef]
  19. Schlesinger, M.J.; Bond, U. Ubiquitin genes. Oxf. Surv. Eukaryot. Gene. 1987, 4, 77–91. [Google Scholar]
  20. Hicke, L.; Schubert, H.L.; Hill, C.P. Ubiquitin-binding domains. Nat. Rev. Mol. Cell. Biol. 2005, 6, 610–621. [Google Scholar] [CrossRef]
  21. Afonso, C.L.; Tulman, E.R.; Lu, Z.; Oma, E.; Kutish, G.F.; Rock, D.L. The genome of Melanoplus sanguinipes entomopoxvirus. J. Virol. 1999, 73, 533–552. [Google Scholar] [CrossRef]
  22. Bawden, A.L.; Glassberg, K.J.; Diggans, J.; Shaw, R.; Farmerie, W.; Moyer, R.W. Complete genomic sequence of the Amsacta moorei entomopoxvirus: Analysis and comparison with other poxviruses. Virology 2000, 274, 120–139. [Google Scholar] [CrossRef] [PubMed]
  23. Tulman, E.R.; Afonso, C.L.; Lu, Z.; Zsak, L.; Kutish, G.F.; Rock, D.L. The genome of canarypox virus. J. Virol. 2004, 78, 353–366. [Google Scholar] [CrossRef] [PubMed]
  24. Afonso, C.L.; Tulman, E.R.; Lu, Z.; Zsak, L.; Kutish, G.F.; Rock, D.L. The genome of fowlpox virus. J. Virol. 2000, 74, 3815–3831. [Google Scholar] [CrossRef] [PubMed]
  25. Reilly, L.M.; Guarino, L.A. The viral ubiquitin gene of Autographa californica nuclear polyhedrosis virus is not essential for viral replication. Virology 1996, 218, 243–247. [Google Scholar] [CrossRef]
  26. Larkin, M.A.; Blackshields, G.; Brown, N.P.; Chenna, R.; McGettigan, P.A.; McWilliam, H.; Valentin, F.; Wallace, I.M.; Wilm, A.; Lopez, R.; Thompson, J.D.; Gibson, T.J.; Higgins, D.G. Clustal W and Clustal X version 2.0. Bioinformatics 2007, 23, 2947–2948. [Google Scholar] [CrossRef]
  27. Thompson, J.D.; Higgins, D.G.; Gibson, T.J. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acid. Res. 1994, 22, 4673–4680. [Google Scholar] [CrossRef]
  28. Lefkowitz, E.J.; Upton, C.; Changayil, S.S.; Buck, C.; Traktman, P.; Buller, R.M. Poxvirus Bioinformatics Resource Center: A comprehensive Poxviridae informational and analytical resource. Nucl. Acid. Res. 2005, 33, D311–D316. [Google Scholar] [CrossRef]
  29. Chung, C.S.; Chen, C.H.; Ho, M.Y.; Huang, C.Y.; Liao, C.L.; Chang, W. Vaccinia virus proteome: Identification of proteins in vaccinia virus intracellular mature virion particles. J. Virol. 2006, 80, 2127–2140. [Google Scholar] [CrossRef]
  30. Guarino, L.A.; Smith, G.; Dong, W. Ubiquitin is attached to membranes of baculovirus particles by a novel type of phospholipid anchor. Cell 1995, 80, 301–309. [Google Scholar] [CrossRef]
  31. Webb, J.H.; Mayer, R.J.; Dixon, L.K. A lipid modified ubiquitin is packaged into particles of several enveloped viruses. FEBS Lett. 1999, 444, 136–139. [Google Scholar] [CrossRef]
  32. Teale, A.; Campbell, S.; Van Buuren, N.; Magee, W.C.; Watmough, K.; Couturier, B.; Shipclark, R.; Barry, M. Orthopoxviruses require a functional ubiquitin-proteasome system for productive replication. J. Virol. 2009, 83, 2099–2108. [Google Scholar] [CrossRef]
  33. Satheshkumar, P.S.; Anton, L.C.; Sanz, P.; Moss, B. Inhibition of the ubiquitin-proteasome system prevents vaccinia virus DNA replication and expression of intermediate and late genes. J. Virol. 2009, 83, 2469–2479. [Google Scholar] [CrossRef]
  34. Haas, A.L.; Katzung, D.J.; Reback, P.M.; Guarino, L.A. Functional characterization of the ubiquitin variant encoded by the baculovirus Autographa californica. Biochemistry 1996, 35, 5385–5394. [Google Scholar] [CrossRef]
  35. Guerin, J.L.; Gelfi, J.; Boullier, S.; Delverdier, M.; Bellanger, F.A.; Bertagnoli, S.; Drexler, I.; Sutter, G.; Messud-Petit, F. Myxoma virus leukemia-associated protein is responsible for major histocompatibility complex class I and Fas-CD95 down-regulation and defines scrapins, a new group of surface cellular receptor abductor proteins. J. Virol. 2002, 76, 2912–2923. [Google Scholar] [CrossRef]
  36. Huang, J.; Huang, Q.; Zhou, X.; Shen, M.M.; Yen, A.; Yu, S.X.; Dong, G.; Qu, K.; Huang, P.; Anderson, E.M.; Daniel-Issakani, S.; Buller, R.M.; Payan, D.G.; Lu, H.H. The poxvirus p28 virulence factor is an E3 ubiquitin ligase. J. Biol. Chem. 2004, 279, 54110–54116. [Google Scholar] [CrossRef]
  37. Mansouri, M.; Bartee, E.; Gouveia, K.; Hovey Nerenberg, B.T.; Barrett, J.; Thomas, L.; Thomas, G.; McFadden, G.; Fruh, K. The PHD/LAP-domain protein M153R of myxomavirus is a ubiquitin ligase that induces the rapid internalization and lysosomal destruction of CD4. J. Virol. 2003, 77, 1427–1440. [Google Scholar] [CrossRef]
  38. Nerenberg, B.T.; Taylor, J.; Bartee, E.; Gouveia, K.; Barry, M.; Fruh, K. The poxviral RING protein p28 is a ubiquitin ligase that targets ubiquitin to viral replication factories. J. Virol. 2005, 79, 597–601. [Google Scholar] [CrossRef]
  39. Nathan, J.A.; Lehner, P.J. The trafficking and regulation of membrane receptors by the RING-CH ubiquitin E3 ligases. Exp. Cell Res. 2009, 315, 1593–1600. [Google Scholar] [CrossRef]
  40. Fruh, K.; Bartee, E.; Gouveia, K.; Mansouri, M. Immune evasion by a novel family of viral PHD/LAP-finger proteins of gamma-2 herpesviruses and poxviruses. Virus Res. 2002, 88, 55–69. [Google Scholar] [CrossRef]
  41. Bartee, E.; Mansouri, M.; Hovey Nerenberg, B.T.; Gouveia, K.; Fruh, K. Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins. J. Virol. 2004, 78, 1109–1120. [Google Scholar] [CrossRef]
  42. Boshkov, L.K.; Macen, J.L.; McFadden, G. Virus-induced loss of class I MHC antigens from the surface of cells infected with myxoma virus and malignant rabbit fibroma virus. J. Immunol. 1992, 148, 881–887. [Google Scholar] [CrossRef] [PubMed]
  43. Zuniga, M.C.; Wang, H.; Barry, M.; McFadden, G. Endosomal/lysosomal retention and degradation of major histocompatibility complex class I molecules is induced by myxoma virus. Virology 1999, 261, 180–192. [Google Scholar] [CrossRef] [PubMed]
  44. Senkevich, T.G.; Koonin, E.V.; Buller, R.M. A poxvirus protein with a RING zinc finger motif is of crucial importance for virulence. Virology 1994, 198, 118–128. [Google Scholar] [CrossRef] [PubMed]
  45. Senkevich, T.G.; Wolffe, E.J.; Buller, R.M. Ectromelia virus RING finger protein is localized in virus factories and is required for virus replication in macrophages. J. Virol. 1995, 69, 4103–4111. [Google Scholar] [CrossRef] [PubMed]
  46. Iyer, L.M.; Koonin, E.V.; Aravind, L. Extensive domain shuffling in transcription regulators of DNA viruses and implications for the origin of fungal APSES transcription factors. Genome Biol. 2002, 3, RESEARCH0012. [Google Scholar] [CrossRef]
  47. Brick, D.J.; Burke, R.D.; Schiff, L.; Upton, C. Shope fibroma virus RING finger protein N1R binds DNA and inhibits apoptosis. Virology 1998, 249, 42–51. [Google Scholar] [CrossRef]
  48. Mottet, K. University of Alberta, Edmonton, Canada. Unpublished work. 2010. [Google Scholar]
  49. Nicholls, R.D.; Gray, T.A. Cellular source of the poxviral N1R/p28 gene family. Virus Genes 2004, 29, 359–364. [Google Scholar] [CrossRef]
  50. Brick, D.J.; Burke, R.D.; Minkley, A.A.; Upton, C. Ectromelia virus virulence factor p28 acts upstream of caspase-3 in response to UV light-induced apoptosis. J. Gen. Virol. 2000, 81, 1087–1097. [Google Scholar] [CrossRef]
  51. Bardwell, V.J.; Treisman, R. The POZ domain: a conserved protein-protein interaction motif. Genes Dev. 1994, 8, 1664–1677. [Google Scholar] [CrossRef]
  52. Melnick, A.; Ahmad, K.F.; Arai, S.; Polinger, A.; Ball, H.; Borden, K.L.; Carlile, G.W.; Prive, G.G.; Licht, J.D. In-depth mutational analysis of the promyelocytic leukemia zinc finger BTB/POZ domain reveals motifs and residues required for biological and transcriptional functions. Mol. Cell. Biol. 2000, 20, 6550–6567. [Google Scholar] [CrossRef]
  53. Kang, M.I.; Kobayashi, A.; Wakabayashi, N.; Kim, S.G.; Yamamoto, M. Scaffolding of Keap1 to the actin cytoskeleton controls the function of Nrf2 as key regulator of cytoprotective phase 2 genes. Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 2046–2051. [Google Scholar] [CrossRef]
  54. Furukawa, M.; He, Y.J.; Borchers, C.; Xiong, Y. Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. Nat. Cell Biol. 2003, 5, 1001–1007. [Google Scholar] [CrossRef]
  55. Geyer, R.; Wee, S.; Anderson, S.; Yates, J.; Wolf, D.A. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. Mol. Cell 2003, 12, 783–790. [Google Scholar] [CrossRef]
  56. Pintard, L.; Willis, J.H.; Willems, A.; Johnson, J.L.; Srayko, M.; Kurz, T.; Glaser, S.; Mains, P.E.; Tyers, M.; Bowerman, B.; Peter, M. The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase. Nature 2003, 425, 311–316. [Google Scholar] [CrossRef]
  57. Xu, L.; Wei, Y.; Reboul, J.; Vaglio, P.; Shin, T.H.; Vidal, M.; Elledge, S.J.; Harper, J.W. BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3. Nature 2003, 425, 316–321. [Google Scholar] [CrossRef]
  58. Aravind, L.; Koonin, E.V. Fold prediction and evolutionary analysis of the POZ domain: Structural and evolutionary relationship with the potassium channel tetramerization domain. J. Mol. Biol. 1999, 285, 1353–1361. [Google Scholar] [CrossRef]
  59. Schulman, B.A.; Carrano, A.C.; Jeffrey, P.D.; Bowen, Z.; Kinnucan, E.R.; Finnin, M.S.; Elledge, S.J.; Harper, J.W.; Pagano, M.; Pavletich, N.P. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature 2000, 408, 381–386. [Google Scholar] [CrossRef]
  60. Adams, J.; Kelso, R.; Cooley, L. The kelch repeat superfamily of proteins: Propellers of cell function. Trends Cell Biol. 2000, 10, 17–24. [Google Scholar] [CrossRef]
  61. Shchelkunov, S.; Totmenin, A.; Kolosova, I. Species-specific differences in organization of orthopoxvirus kelch-like proteins. Virus Genes 2002, 24, 157–162. [Google Scholar] [CrossRef]
  62. Kotwal, G.J.; Moss, B. Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant. Virology 1988, 167, 524–537. [Google Scholar] [CrossRef]
  63. Shchelkunov, S.N.; Safronov, P.F.; Totmenin, A.V.; Petrov, N.A.; Ryazankina, O.I.; Gutorov, V.V.; Kotwal, G.J. The genomic sequence analysis of the left and right species-specific terminal region of a cowpox virus strain reveals unique sequences and a cluster of intact ORFs for immunomodulatory and host range proteins. Virology 1998, 243, 432–460. [Google Scholar] [CrossRef] [PubMed]
  64. Chen, N.; Danila, M.I.; Feng, Z.; Buller, R.M.; Wang, C.; Han, X.; Lefkowitz, E.J.; Upton, C. The genomic sequence of ectromelia virus, the causative agent of mousepox. Virology 2003, 317, 165–186. [Google Scholar] [CrossRef] [PubMed]
  65. Shchelkunov, S.N.; Totmenin, A.V.; Safronov, P.F.; Mikheev, M.V.; Gutorov, V.V.; Ryazankina, O.I.; Petrov, N.A.; Babkin, I.V.; Uvarova, E.A.; Sandakhchiev, L.S.; Sisler, J.R.; Esposito, J.J.; Damon, I.K.; Jahrling, P.B.; Moss, B. Analysis of the monkeypox virus genome. Virology 2002, 297, 172–194. [Google Scholar] [CrossRef] [PubMed]
  66. Wilton, B.A.; Campbell, S.; Van Buuren, N.; Garneau, R.; Furukawa, M.; Xiong, Y.; Barry, M. Ectromelia virus BTB/kelch proteins, EVM150 and EVM167, interact with cullin-3-based ubiquitin ligases. Virology 2008, 374, 82–99. [Google Scholar] [CrossRef] [PubMed]
  67. Wilton, B. University of Alberta, Edmonton, Canada. Unpublished work. 2009. [Google Scholar]
  68. Pires de Miranda, M.; Reading, P.C.; Tscharke, D.C.; Murphy, B.J.; Smith, G.L. The vaccinia virus kelch-like protein C2L affects calcium-independent adhesion to the extracellular matrix and inflammation in a murine intradermal model. J. Gen. Virol. 2003, 84, 2459–2471. [Google Scholar] [CrossRef]
  69. Beard, P.M.; Froggatt, G.C.; Smith, G.L. Vaccinia virus kelch protein A55 is a 64 kDa intracellular factor that affects virus-induced cytopathic effect and the outcome of infection in a murine intradermal model. J. Gen. Virol. 2006, 87, 1521–1529. [Google Scholar] [CrossRef]
  70. Froggatt, G.C.; Smith, G.L.; Beard, P.M. Vaccinia virus gene F3L encodes an intracellular protein that affects the innate immune response. J. Gen. Virol. 2007, 88, 1917–1921. [Google Scholar] [CrossRef]
  71. Kochneva, G.; Kolosova, I.; Maksyutova, T.; Ryabchikova, E.; Shchelkunov, S. Effects of deletions of kelch-like genes on cowpox virus biological properties. Arch. Virol. 2005, 150, 1857–1870. [Google Scholar] [CrossRef]
  72. Balinsky, C.A.; Delhon, G.; Afonso, C.L.; Risatti, G.R.; Borca, M.V.; French, R.A.; Tulman, E.R.; Geary, S.J.; Rock, D.L. Sheeppox virus kelch-like gene SPPV-019 affects virus virulence. J. Virol. 2007, 81, 11392–11401. [Google Scholar] [CrossRef]
  73. Cullinan, S.B.; Gordan, J.D.; Jin, J.; Harper, J.W.; Diehl, J.A. The Keap1-BTB protein is an adaptor that bridges Nrf2 to a Cul3-based E3 ligase: Oxidative stress sensing by a Cul3-Keap1 ligase. Mol. Cell. Biol. 2004, 24, 8477–8486. [Google Scholar] [CrossRef]
  74. Furukawa, M.; Xiong, Y. BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase. Mol. Cell. Biol. 2005, 25, 162–171. [Google Scholar] [CrossRef] [PubMed]
  75. Lee, D.F.; Kuo, H.P.; Liu, M.; Chou, C.K.; Xia, W.; Du, Y.; Shen, J.; Chen, C.T.; Huo, L.; Hsu, M.C.; Li, C.W.; Ding, Q.; Liao, T.L.; Lai, C.C.; Lin, A.C.; Chang, Y.H.; Tsai, S.F.; Li, L.Y.; Hung, M.C. KEAP1 E3 ligase-mediated downregulation of NF-kappaB signaling by targeting IKKbeta. Mol. Cell 2009, 36, 131–140. [Google Scholar] [CrossRef] [PubMed]
  76. Maerki, S.; Olma, M.H.; Staubli, T.; Steigemann, P.; Gerlich, D.W.; Quadroni, M.; Sumara, I.; Peter, M. The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis. J. Cell Biol. 2009, 187, 791–800. [Google Scholar] [CrossRef] [PubMed]
  77. Zhang, L.; Villa, N.Y.; Rahman, M.M.; Smallwood, S.; Shattuck, D.; Neff, C.; Dufford, M.; Lanchbury, J.S.; Labaer, J.; McFadden, G. Analysis of vaccinia virus-host protein-protein interactions: Validations of yeast two-hybrid screenings. J. Proteome Res. 2009, 8, 4311–4318. [Google Scholar] [CrossRef] [PubMed]
  78. Al-Khodor, S.; Price, C.T.; Kalia, A.; Abu Kwaik, Y. Functional diversity of ankyrin repeats in microbial proteins. Trends Microbiol. 2010, 18, 132–139. [Google Scholar] [CrossRef]
  79. Mosavi, L.K.; Cammett, T.J.; Desrosiers, D.C.; Peng, Z.Y. The ankyrin repeat as molecular architecture for protein recognition. Protein Sci. 2004, 13, 1435–1448. [Google Scholar] [CrossRef]
  80. Sedgwick, S.G.; Smerdon, S.J. The ankyrin repeat: A diversity of interactions on a common structural framework. Trends Biochem. Sci. 1999, 24, 311–316. [Google Scholar] [CrossRef]
  81. Lux, S.E.; John, K.M.; Bennett, V. Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins. Nature 1990, 344, 36–42. [Google Scholar] [CrossRef]
  82. Mercer, A.A.; Fleming, S.B.; Ueda, N. F-box-like domains are present in most poxvirus ankyrin repeat proteins. Virus Genes 2005, 31, 127–133. [Google Scholar] [CrossRef]
  83. Skowyra, D.; Craig, K.L.; Tyers, M.; Elledge, S.J.; Harper, J.W. F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 1997, 91, 209–219. [Google Scholar] [CrossRef]
  84. Zheng, N.; Schulman, B.A.; Song, L.; Miller, J.J.; Jeffrey, P.D.; Wang, P.; Chu, C.; Koepp, D.M.; Elledge, S.J.; Pagano, M.; Conaway, R.C.; Conaway, J.W.; Harper, J.W.; Pavletich, N.P. Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex. Nature 2002, 416, 703–709. [Google Scholar] [CrossRef]
  85. Cardozo, T.; Pagano, M. The SCF ubiquitin ligase: Insights into a molecular machine. Nat. Rev. Mol. Cell. Biol. 2004, 5, 739–751. [Google Scholar] [CrossRef]
  86. Petroski, M.D.; Deshaies, R.J. Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell. Biol. 2005, 6, 9–20. [Google Scholar] [CrossRef]
  87. Werren, J.H.; Richards, S.; Desjardins, C.A.; Niehuis, O.; Gadau, J.; Colbourne, J.K.; Beukeboom, L.W.; Desplan, C.; Elsik, C.G.; Grimmelikhuijzen, C.J.; et al. Functional and evolutionary insights from the genomes of three parasitoid Nasonia species. Science 2010, 327, 343–348. [Google Scholar] [CrossRef] [PubMed]
  88. Sonnberg, S.; Fleming, S.B.; Mercer, A.A. A truncated two-alpha-helix F-box present in poxvirus ankyrin-repeat proteins is sufficient for binding the SCF1 ubiquitin ligase complex. J. Gen. Virol. 2009, 90, 1224–1228. [Google Scholar] [CrossRef]
  89. Van Buuren, N.; Couturier, B.; Xiong, Y.; Barry, M. Ectromelia virus encodes a novel family of F-box proteins that interact with the SCF complex. J. Virol. 2008, 82, 9917–9927. [Google Scholar] [CrossRef]
  90. Chang, S.J.; Hsiao, J.C.; Sonnberg, S.; Chiang, C.T.; Yang, M.H.; Tzou, D.L.; Mercer, A.A.; Chang, W. Poxvirus host range protein CP77 contains an F-box-like domain that is necessary to suppress NF-kappaB activation by tumor necrosis factor alpha but is independent of its host range function. J. Virol. 2009, 83, 4140–4152. [Google Scholar] [CrossRef]
  91. Piessevaux, J.; Lavens, D.; Peelman, F.; Tavernier, J. The many faces of the SOCS box. Cytokine Growth Factor Rev. 2008, 19, 371–381. [Google Scholar] [CrossRef]
  92. Sonnberg, S.; Seet, B.T.; Pawson, T.; Fleming, S.B.; Mercer, A.A. Poxvirus ankyrin repeat proteins are a unique class of F-box proteins that associate with cellular SCF1 ubiquitin ligase complexes. Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 10955–10960. [Google Scholar] [CrossRef]
  93. Meng, X.; Xiang, Y. Vaccinia virus K1L protein supports viral replication in human and rabbit cells through a cell-type-specific set of its ankyrin repeat residues that are distinct from its binding site for ACAP2. Virology 2006, 353, 220–233. [Google Scholar] [CrossRef]
  94. Shisler, J.L.; Jin, X.L. The vaccinia virus K1L gene product inhibits host NF-kappaB activation by preventing IkappaBalpha degradation. J. Virol. 2004, 78, 3553–3560. [Google Scholar] [CrossRef] [PubMed]
  95. Johnston, J.B.; Wang, G.; Barrett, J.W.; Nazarian, S.H.; Colwill, K.; Moran, M.; McFadden, G. Myxoma virus M-T5 protects infected cells from the stress of cell cycle arrest through its interaction with host cell cullin-1. J. Virol. 2005, 79, 10750–10763. [Google Scholar] [CrossRef] [PubMed]
  96. Werden, S.J.; Lanchbury, J.; Shattuck, D.; Neff, C.; Dufford, M.; McFadden, G. The myxoma virus m-t5 ankyrin repeat host range protein is a novel adaptor that coordinately links the cellular signaling pathways mediated by Akt and Skp1 in virus-infected cells. J. Virol. 2009, 83, 12068–12083. [Google Scholar] [CrossRef]
  97. Blanie, S.; Mortier, J.; Delverdier, M.; Bertagnoli, S.; Camus-Bouclainville, C. M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit. Vet. Res. 2009, 40, 11. [Google Scholar] [CrossRef]
  98. Mossman, K.; Lee, S.F.; Barry, M.; Boshkov, L.; McFadden, G. Disruption of M-T5, a novel myxoma virus gene member of poxvirus host range superfamily, results in dramatic attenuation of myxomatosis in infected European rabbits. J. Virol. 1996, 70, 4394–4410. [Google Scholar] [CrossRef]
  99. Camus-Bouclainville, C.; Fiette, L.; Bouchiha, S.; Pignolet, B.; Counor, D.; Filipe, C.; Gelfi, J.; Messud-Petit, F. A virulence factor of myxoma virus colocalizes with NF-kappaB in the nucleus and interferes with inflammation. J. Virol. 2004, 78, 2510–2516. [Google Scholar] [CrossRef]
  100. Sperling, K.M.; Schwantes, A.; Schnierle, B.S.; Sutter, G. The highly conserved orthopoxvirus 68k ankyrin-like protein is part of a cellular SCF ubiquitin ligase complex. Virology 2008, 374, 234–239. [Google Scholar] [CrossRef]
  101. Hsiao, J.C.; Chao, C.C.; Young, M.J.; Chang, Y.T.; Cho, E.C.; Chang, W. A poxvirus host range protein, CP77, binds to a cellular protein, HMG20A, and regulates its dissociation from the vaccinia virus genome in CHO-K1 cells. J. Virol. 2006, 80, 7714–7728. [Google Scholar] [CrossRef]
  102. Mohamed, M.R.; Rahman, M.M.; Lanchbury, J.S.; Shattuck, D.; Neff, C.; Dufford, M.; Van Buuren, N.; Fagan, K.; Barry, M.; Smith, S.; Damon, I.; McFadden, G. Proteomic screening of variola virus reveals a unique NF-kappaB inhibitor that is highly conserved among pathogenic orthopoxviruses. Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 9045–9050. [Google Scholar] [CrossRef]
  103. Mohamed, M.R.; Rahman, M.M.; Rice, A.; Moyer, R.W.; Werden, S.J.; McFadden, G. Cowpox virus expresses a novel ankyrin repeat NF-kappaB inhibitor that controls inflammatory cell influx into virus-infected tissues and is critical for virus pathogenesis. J. Virol. 2009, 83, 9223–9236. [Google Scholar] [CrossRef]
  104. Peters, J.M. The anaphase promoting complex/cyclosome: A machine designed to destroy. Nat. Rev. Mol. Cell. Biol. 2006, 7, 644–656. [Google Scholar] [CrossRef] [PubMed]
  105. Tang, Z.; Li, B.; Bharadwaj, R.; Zhu, H.; Ozkan, E.; Hakala, K.; Deisenhofer, J.; Yu, H. APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. Mol. Biol. Cell. 2001, 12, 3839–3851. [Google Scholar] [CrossRef] [PubMed]
  106. Leverson, J.D.; Joazeiro, C.A.; Page, A.M.; Huang, H.; Hieter, P.; Hunter, T. The APC11 RING-H2 finger mediates E2-dependent ubiquitination. Mol. Biol. Cell. 2000, 11, 2315–2325. [Google Scholar] [CrossRef] [PubMed]
  107. Glotzer, M.; Murray, A.W.; Kirschner, M.W. Cyclin is degraded by the ubiquitin pathway. Nature 1991, 349, 132–138. [Google Scholar] [CrossRef] [PubMed]
  108. Pfleger, C.M.; Kirschner, M.W. The KEN box: An APC recognition signal distinct from the D box targeted by Cdh1. Genes Dev. 2000, 14, 655–665. [Google Scholar] [CrossRef] [PubMed]
  109. Nasmyth, K. Disseminating the genome: Joining, resolving, and separating sister chromatids during mitosis and meiosis. Annu. Rev. Genet. 2001, 35, 673–745. [Google Scholar] [CrossRef]
  110. Mo, M.; Fleming, S.B.; Mercer, A.A. Cell cycle deregulation by a poxvirus partial mimic of anaphase-promoting complex subunit 11. Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 19527–19532. [Google Scholar] [CrossRef]
  111. Johnston, J.B.; McFadden, G. Poxvirus immunomodulatory strategies: current perspectives. J. Virol. 2003, 77, 6093–6100. [Google Scholar] [CrossRef]
  112. Mimnaugh, E.G.; Chen, H.Y.; Davie, J.R.; Celis, J.E.; Neckers, L. Rapid deubiquitination of nucleosomal histones in human tumor cells caused by proteasome inhibitors and stress response inducers: effects on replication, transcription, translation, and the cellular stress response. Biochemistry 1997, 36, 14418–14429. [Google Scholar] [CrossRef]
  113. Lee, D.H.; Goldberg, A.L. Proteasome inhibitors: Valuable new tools for cell biologists. Trends Cell Biol. 1998, 8, 397–403. [Google Scholar] [CrossRef]
  114. Myung, J.; Kim, K.B.; Crews, C.M. The ubiquitin-proteasome pathway and proteasome inhibitors. Med. Res. Rev. 2001, 21, 245–273. [Google Scholar] [CrossRef]
  115. Einsele, H. Bortezomib. Recent Results Cancer Res. 2010, 184, 173–187. [Google Scholar]
  116. De Silva, F.S.; Moss, B. Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors. Virol. J. 2005, 2, 23. [Google Scholar] [CrossRef]
  117. Schubert, U.; Ott, D.E.; Chertova, E.N.; Welker, R.; Tessmer, U.; Princiotta, M.F.; Bennink, J.R.; Krausslich, H.G.; Yewdell, J.W. Proteasome inhibition interferes with gag polyprotein processing, release, and maturation of HIV-1 and HIV-2. Proc. Natl. Acad. Sci. U. S. A. 2000, 97, 13057–13062. [Google Scholar] [CrossRef]
  118. Dudek, S.E.; Luig, C.; Pauli, E.K.; Schubert, U.; Ludwig, S. The clinically approved proteasome inhibitor PS-341 efficiently blocks influenza A virus and vesicular stomatitis virus propagation by establishing an antiviral state. J. Virol. 2010, 84, 9439–9451. [Google Scholar] [CrossRef]
  119. Raaben, M.; Posthuma, C.C.; Verheije, M.H.; Te Lintelo, E.G.; Kikkert, M.; Drijfhout, J.W.; Snijder, E.J.; Rottier, P.J.; de Haan, C.A. The ubiquitin-proteasome system plays an important role during various stages of the coronavirus infection cycle. J. Virol. 2010, 84, 7869–7879. [Google Scholar] [CrossRef]
  120. Prosch, S.; Priemer, C.; Hoflich, C.; Liebenthaf, C.; Babel, N.; Kruger, D.H.; Volk, H.D. Proteasome inhibitors: A novel tool to suppress human cytomegalovirus replication and virus-induced immune modulation. Antivir. Ther. 2003, 8, 555–567. [Google Scholar] [CrossRef]
  121. Lupfer, C.; Pastey, M.K. Decreased replication of human respiratory syncytial virus treated with the proteasome inhibitor MG-132. Virus Res. 2010, 149, 36–41. [Google Scholar] [CrossRef]
  122. Delboy, M.G.; Roller, D.G.; Nicola, A.V. Cellular proteasome activity facilitates herpes simplex virus entry at a postpenetration step. J. Virol. 2008, 82, 3381–3390. [Google Scholar] [CrossRef]
  123. Bandi, P.; Garcia, M.L.; Booth, C.J.; Chisari, F.V.; Robek, M.D. Bortezomib inhibits hepatitis B virus replication in transgenic mice. Antimicrob. Agents Chemother. 2010, 54, 749–756. [Google Scholar] [CrossRef]
  124. Basler, M.; Lauer, C.; Beck, U.; Groettrup, M. The proteasome inhibitor bortezomib enhances the susceptibility to viral infection. J. Immunol. 2009, 183, 6145–6150. [Google Scholar] [CrossRef] [PubMed]
  125. Fagan-Garcia, K. University of Alberta, Edmonton, Canada. Unpublished work. 2010. [Google Scholar]
  126. Han, X.; Aslanian, A.; Yates, J.R., 3rd. Mass spectrometry for proteomics. Curr. Opin. Chem. Biol. 2008, 12, 483–490. [Google Scholar] [CrossRef]
  127. Kirkpatrick, D.S.; Denison, C.; Gygi, S.P. Weighing in on ubiquitin: The expanding role of mass-spectrometry-based proteomics. Nat. Cell Biol. 2005, 7, 750–757. [Google Scholar] [CrossRef] [PubMed]
  128. Rigbolt, K.T.; Blagoev, B. Proteome-wide quantitation by SILAC. Methods Mol. Biol. 2010, 658, 187–204. [Google Scholar]
  129. Yates, J.R.; Ruse, C.I.; Nakorchevsky, A. Proteomics by mass spectrometry: Approaches, advances, and applications. Annu. Rev. Biomed. Eng. 2009, 11, 49–79. [Google Scholar] [CrossRef] [PubMed]
© 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
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Figure 1. Poxvirus Encoded Ubiquitin. Amino acid sequences of MSEV144, AMEV180, CNPV096 and human ubiquitin were aligned using Clustal W [26,27]. Poxvirus amino acid sequences were obtained from the Poxvirus Bioinformatics Resource Center [28]. Residues representing 100% conservation are shaded.
Figure 1. Poxvirus Encoded Ubiquitin. Amino acid sequences of MSEV144, AMEV180, CNPV096 and human ubiquitin were aligned using Clustal W [26,27]. Poxvirus amino acid sequences were obtained from the Poxvirus Bioinformatics Resource Center [28]. Residues representing 100% conservation are shaded.
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Viruses 02 02356 g002
Figure 2. Poxvirus Encoded Ubiquitin Ligases. (A) Membrane associated RING-CH (MARCH) ubiquitin ligase. The MARCH ubiquitin ligase, M153R, encoded by myxoma virus contains two transmembrane domains and a C-terminal RING-CH domain. (B) p28, a RING Ubiquitin Ligase. p28 contains a C-terminal RING domain and an N-terminal KilA-N DNA binding domain. (C) BTB/Kelch ubiquitin ligases. BTB/Kelch proteins interact with cullin-3 through their BTB domain. Potential substrates are likely recruited through the kelch domain. (D) Ank/PRANC ubiquitin ligases. Cullin-1 interacts with Skp-1, which in turn interacts with Ank/PRANC proteins. The Ank domain potentially interacts with substrates recruiting them to the cullin-1 ubiquitin ligase. (E) Anaphase Promoting Complex (APC). It is hypothesized that PACR displaces APC11 subsequently disrupting APC function.
Figure 2. Poxvirus Encoded Ubiquitin Ligases. (A) Membrane associated RING-CH (MARCH) ubiquitin ligase. The MARCH ubiquitin ligase, M153R, encoded by myxoma virus contains two transmembrane domains and a C-terminal RING-CH domain. (B) p28, a RING Ubiquitin Ligase. p28 contains a C-terminal RING domain and an N-terminal KilA-N DNA binding domain. (C) BTB/Kelch ubiquitin ligases. BTB/Kelch proteins interact with cullin-3 through their BTB domain. Potential substrates are likely recruited through the kelch domain. (D) Ank/PRANC ubiquitin ligases. Cullin-1 interacts with Skp-1, which in turn interacts with Ank/PRANC proteins. The Ank domain potentially interacts with substrates recruiting them to the cullin-1 ubiquitin ligase. (E) Anaphase Promoting Complex (APC). It is hypothesized that PACR displaces APC11 subsequently disrupting APC function.
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Viruses 02 02356 g003
Figure 3. Sequence alignment of ectromelia virus encoded Ank/PRANC proteins with cellular Skp2: AlignX was used to align the C-termini of EVM002, EVM005, EVM154, and EVM165 with the N-terminal F-box domain of Skp2, a cellular F-box protein [89]. Red dots indicate known contact points between Skp2 and Skp1 [59]. H1, H2, and H3 represent alpha-helical secondary structures from Skp2.
Figure 3. Sequence alignment of ectromelia virus encoded Ank/PRANC proteins with cellular Skp2: AlignX was used to align the C-termini of EVM002, EVM005, EVM154, and EVM165 with the N-terminal F-box domain of Skp2, a cellular F-box protein [89]. Red dots indicate known contact points between Skp2 and Skp1 [59]. H1, H2, and H3 represent alpha-helical secondary structures from Skp2.
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Table 1. Poxvirus encoded modulators of the ubiquitin-proteasome system. Poxviruses are known to encode a number of modulators of the ubiquitin-proteasome system. Included in this table are ubiquitin homologs encoded by poxviruses, as well as MARCH and p28 E3 ubiquitin ligases, BTB/kelch and Ank/PRANC proteins that associate with cellular ubiquitin ligases, and poxvirus APC/cyclosome regulators.
Table 1. Poxvirus encoded modulators of the ubiquitin-proteasome system. Poxviruses are known to encode a number of modulators of the ubiquitin-proteasome system. Included in this table are ubiquitin homologs encoded by poxviruses, as well as MARCH and p28 E3 ubiquitin ligases, BTB/kelch and Ank/PRANC proteins that associate with cellular ubiquitin ligases, and poxvirus APC/cyclosome regulators.
Involvement in UbiquitinationGenusVirusaGene/ProteinLength, aaVBRC accessionb
poxvirus-encoded ubiquitin homologsAvipoxvirusCNPV-VR1119685VP0043569
BetaentomopoxvirusAMEV-Moyer18081VP0037620
Unclassified PoxviridaeMSEV-Tuc14480VP0038302
MARCH Poxviral E3 Ubiquitin LigaseCapripoxvirusGTPV-Pellor8162VP0044818
LSDV-Nee10162VP0040213
SPPV-A8162VP0044517
LeporipoxvirusMYXV-LauM153206VP0038581
RFV-Kasgp153R201VP0038747
SuipoxvirusSWPV-Neb9155VP0040564
YatapoxvirusTANV-COD5156VP0067544
YLDV-Davis5156VP0040054
YMTV-Amano4156VP0043053
p28 Poxviral E3 Ubiquitin LigaseAvipoxvirusCNPV-VR111205318VP0043678
197275VP0043670
FWPV-Iowa157311VP0037889
150276VP0037882
CapripoxvirusLSDV-Nee140240VP0040345
SPPV-A136240VP0044645
GTPV-Pellor127240VP0044947
LeporipoxvirusMYXV-LauM143234VP0038572
RFV-Kasgp143R (N1R)234VP0038740
OrthopoxvirusCMLV-CMS14R242VP0041112
CPXV-GRIC7R242VP0042678
ECTV-Mos12241VP0040932
MPXV-ZARD5R242VP0040369
VACV IHD-Wp28243c
VARV-BGD75majD6Rd242VP0038767
RPXV-Utr8242VP0041370
SuipoxvirusSWPV-Neb138246VP0040694
YatapoxvirusTANV-COD143R234VP0067759
YMTV-Amano143R236VP0043181
Unclassified PoxviridaeDPV-W1170_84154245VP0045437
BTB/KELCH proteins associated with cullin-3-based E3 ubiquitin ligaseCapripoxvirusGTPV-Pellor16562VP0044826
141547VP0044951
148552VP0044958
LSDV-NEE19569VP0040222
144547VP0040349
151550VP0040356
SPPV-A16569VP0044525
140547VP0044649
147552VP0044656
LeporipoxvirusMYXV-LauM014L517VP0038442
M140R553VP0038569
RFV-Kasgp013L516VP0038613
gp0140R553VP0038737
OrthopoxvirusCMLV-CMS21L200VP0041119
24L512VP0041122
38L480VP0041137
172R564VP0041317
186R501VP0041335
CPXV-GRID11L521VP0042668
C18L512VP0042689
G3L485VP0042703
A54R564VP0042838
B9R501VP0042849
B19R557VP0042686
ECTV-Mos18512VP0040938
27482VP0040947
150563VP0041074
165594VP0041089
MPXV-ZAID12L206VP0040376
D19L107VP0040382
C9L487VP0040396
TATV-DAH6824150VP0052942
43480VP0052961
181219VP0053099
196209VP0053114
VACV-COPC2L512VP0039555
C5L615VP0039551
F3L480VP0039572
A55R564VP0039751
RPXV-Utr15204VP0041377
18512VP0041380
31480VP0041393
162564VP0041526
SuipoxvirusSWPV-Neb6530VP0040561
15534VP0040570
136574VP0040692
YatapoxvirusYLDV-Davis19L522VP0040068
140R570VP0040192
YMTV-Amano19L524VP0043062
Unclassified PoxviridaeDPV-W1170_8425529VP0045308
159546VP0045442
ankyrin/PRANC proteins associated with cullin-1-based E3 ubiquitin ligase AvipoxvirusFWPV-Iowa12331VP0037744
14437VP0037746
18700VP0037750
22578VP0037754
26436VP0037758
31341VP0037763
162603VP0037894
218461VP0037952
219434VP0037953
222747VP0037956
227361VP0037961
228525VP0037962
231256VP0037965
232482VP0037966
233512VP0037967
234428VP0037968
240410VP0037974
243262VP0037977
244668VP0037978
246592VP0037980
CapripoxvirusGTPV-Pellor142634VP0044952
144498VP0044954
145447VP0044955
149453VP0044959
LSDV-Nee145634VP0040350
147498VP0040352
148447VP0040353
152489VP0042090
SPPV-A141631VP0044650
143498VP0044652
144447VP0044653
148484VP0044657
LeporipoxvirusMYXV-Lau148R675VP0038576
149R490VP0038577
150R494VP0038578
005R (MT-5)483VP0038588
OrthopoxvirusCMLV-CMS3L585VP0041099
4L672VP0041101
177L564VP0041325
197R783VP0041349
CPXV-GRID3L586VP0042660
D4L672VP0042661
D8L (CP77)661VP0042665
C1L437VP0042672
C11L614VP0042682
B3R558VP0042843
B16R574VP0042856
B18R795VP0042858
K1R581VP0042863
I2R672VP0042868
I3R586VP0042869
ECTV-Mos2587VP0040921
5650VP0040924
154564VP0041078
165594VP0041089
MPXV-ZARB5R561VP0040530
J1R587VP0040553
N4R437VP0040552
B17R793VP0040542
TATV-DAH68220640VP0053138
187558VP0053105
18661VP0052936
6627VP0052924
VACV-CopB18R574VP0039778
C19L259VP0039532
B4R558VP0039761
RPXV-Utr180791VP0041544
178574VP0041542
166558VP0041530
VARV-BDG75majB5R558VP0038933
G1R585VP0039159
B16R574VP0038944
B18R787VP0038946
ParapoxvirusORFV-NZ28516VP0047660
123525VP0047777
126497VP0047780
128500VP0047782
129520VP0047783
SuipoxvirusSWPV-Neb141635VP0040697
142485VP0040698
143430VP0040699
144493VP0040700
YatapoxvirusYLDV-Davis148R476VP0040200
147R491VP0040199
146R473VP0040198
11L637VP0040060
YMTV-Amano11L637VP0043056
146R356VP0043184
147R497VP0043185
148R483VP0043186
Unclassified PoxviridaeDPV-W1170_84164493VP0045447
163483VP0045446
162501VP0045445
160641VP0045443
19643VP0045302
poxvirus APC/cyclosome regulatorsMolluscipoxvirusMOCV-st1026L83VP0038021
ParapoxvirusBSPV-AR021393VP0043354
ORFV-NZ21493VP0047667
Unclassified PoxvirdaeCRV-ZWE4781VP0066074
SPVA11L86DQ377804e
a Representative strains were chosen for each individual virus, and the viruses are abbreviated: Canarypox virus (CNPV), Fowlpox virus (FWPV), Goatpox virus (GTPV), Lumpy skin disease virus (LSDV), Sheepox virus (SPPV), Myxoma virus (MYXV), Rabbit fibroma virus (RFV), Molluscum contagiosum virus (MOCV), Camelpox virus (CMLV), Cowpox virus (CPXV), Ectromelia virus (ECTV), Monkeypox virus (MPXV), Taterapox virus (TATV), Vaccinia virus (VACV), Variola virus (VARV), Bovine papular stomatitis virus (BPSV), Orf virus (ORFV), Swinepox virus (SWPV), Tanapox virus (TANV), Yaba-like disease virus (YLDV), Yaba monkey tumor virus (YMTV), Amsacta moorei enomopoxvirus (AMEV), Melanoplus sanguinipes entomopoxvirus (MSEV), Mule deer poxvirus (DPV), Nile crocodile poxvirus (CRV), Squirrel poxvirus (SPV). b VBRC accession numbers were obtained from the Poxvirus Bioinformatics Resource Center [28]. c The complete VACV-IHD-W genome has not been published and an accession number is not available. d D6R is also known as D4R, B5R or B6R, depending on the strain of VARV. e The SPV genome is not available in the Poxvirus Bioinformatics Resource Center so the accession number from GENBANK was used.

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Barry, M.; Van Buuren, N.; Burles, K.; Mottet, K.; Wang, Q.; Teale, A. Poxvirus Exploitation of the Ubiquitin-Proteasome System. Viruses 2010, 2, 2356-2380. https://doi.org/10.3390/v2102356

AMA Style

Barry M, Van Buuren N, Burles K, Mottet K, Wang Q, Teale A. Poxvirus Exploitation of the Ubiquitin-Proteasome System. Viruses. 2010; 2(10):2356-2380. https://doi.org/10.3390/v2102356

Chicago/Turabian Style

Barry, Michele, Nicholas Van Buuren, Kristin Burles, Kelly Mottet, Qian Wang, and Alastair Teale. 2010. "Poxvirus Exploitation of the Ubiquitin-Proteasome System" Viruses 2, no. 10: 2356-2380. https://doi.org/10.3390/v2102356

APA Style

Barry, M., Van Buuren, N., Burles, K., Mottet, K., Wang, Q., & Teale, A. (2010). Poxvirus Exploitation of the Ubiquitin-Proteasome System. Viruses, 2(10), 2356-2380. https://doi.org/10.3390/v2102356

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