Preventive and Therapeutic Effects of Lactiplantibacillus plantarum HD02 and MD159 through Mast Cell Degranulation Inhibition in Mouse Models of Atopic Dermatitis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Identification of New Probiotic Strains
2.2. Mice
2.3. Mast Cell Degranulation Assay
2.4. Passive Cutaneous Anaphylaxis (PCA) Model
2.5. MC903-Induced AD Model
2.6. House Dust Mite (HDM)-Induced AD Model
2.7. RNA Extraction and Quantitative RT-PCR (qRT-PCR) Analysis
2.8. Enzyme-Linked Immunosorbent Assay (ELISA)
2.9. Flow Cytometry Analysis
2.10. Histological Analysis
2.11. Whole-Genome Sequencing and Analysis
2.12. Statistical Analysis
3. Results
3.1. Identification of L. plantarum HD02 and MD159 as Potent Inhibitors of Mast Cell Degranulation
3.2. Whole-Genome Analysis of L. plantarum HD02 and MD159
3.3. L. plantarum HD02 and MD159 Show Preventive Effects in an MC903-Induced AD Mouse Model
3.4. L. plantarum HD02 and MD159 Show Therapeutic Activity in an HDM-Induced AD Mouse Model
3.5. L. plantarum HD02 and MD159 Regulate Immune Responses in an HDM-Induced AD Mouse Model
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kim, A.-R.; Jeon, S.-G.; Kim, H.-R.; Hong, H.; Yoon, Y.W.; Lee, B.-M.; Yoon, C.H.; Choi, S.J.; Jang, M.H.; Yang, B.-G. Preventive and Therapeutic Effects of Lactiplantibacillus plantarum HD02 and MD159 through Mast Cell Degranulation Inhibition in Mouse Models of Atopic Dermatitis. Nutrients 2024, 16, 3021. https://doi.org/10.3390/nu16173021
Kim A-R, Jeon S-G, Kim H-R, Hong H, Yoon YW, Lee B-M, Yoon CH, Choi SJ, Jang MH, Yang B-G. Preventive and Therapeutic Effects of Lactiplantibacillus plantarum HD02 and MD159 through Mast Cell Degranulation Inhibition in Mouse Models of Atopic Dermatitis. Nutrients. 2024; 16(17):3021. https://doi.org/10.3390/nu16173021
Chicago/Turabian StyleKim, A-Ram, Seong-Gak Jeon, Hyung-Ran Kim, Heeji Hong, Yong Won Yoon, Byung-Min Lee, Chung Hoo Yoon, Soo Jin Choi, Myoung Ho Jang, and Bo-Gie Yang. 2024. "Preventive and Therapeutic Effects of Lactiplantibacillus plantarum HD02 and MD159 through Mast Cell Degranulation Inhibition in Mouse Models of Atopic Dermatitis" Nutrients 16, no. 17: 3021. https://doi.org/10.3390/nu16173021
APA StyleKim, A. -R., Jeon, S. -G., Kim, H. -R., Hong, H., Yoon, Y. W., Lee, B. -M., Yoon, C. H., Choi, S. J., Jang, M. H., & Yang, B. -G. (2024). Preventive and Therapeutic Effects of Lactiplantibacillus plantarum HD02 and MD159 through Mast Cell Degranulation Inhibition in Mouse Models of Atopic Dermatitis. Nutrients, 16(17), 3021. https://doi.org/10.3390/nu16173021