Open AccessArticle
NMR-Metabolic Methodology in the Study of GM Foods
by
Anatoly P. Sobolev, Donatella Capitani, Donato Giannino, Chiara Nicolodi, Giulio Testone, Flavio Santoro, Giovanna Frugis, Maria A. Iannelli, Autar K. Mattoo, Elvino Brosio, Raffaella Gianferri, Irene D’Amico and Luisa Mannina
Cited by 22 | Viewed by 13157
Abstract
The
1H-NMR methodology used in the study of genetically modified (GM) foods is discussed. Transgenic lettuce (
Lactuca sativa cv \"Luxor\") over-expressing the
Arabidopsis KNAT1 gene is presented as a case study. Twenty-two water-soluble metabolites (amino acids, organic acids, sugars) present in
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The
1H-NMR methodology used in the study of genetically modified (GM) foods is discussed. Transgenic lettuce (
Lactuca sativa cv \"Luxor\") over-expressing the
Arabidopsis KNAT1 gene is presented as a case study. Twenty-two water-soluble metabolites (amino acids, organic acids, sugars) present in leaves of conventional and GM lettuce were monitored by NMR and quantified at two developmental stages. The NMR spectra did not reveal any difference in metabolite composition between the GM lettuce and the wild type counterpart. Statistical analyses of metabolite variables highlighted metabolism variation as a function of leaf development as well as the transgene. A main effect of the transgene was in altering sugar metabolism.
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