Correction: Oniciuc, E. A.; et al. The Present and Future of Whole Genome Sequencing (WGS) and Whole Metagenome Sequencing (WMS) for Surveillance of Antimicrobial Resistant Microorganisms and Antimicrobial Resistance Genes across the Food Chain. Genes 2018, 9, 268.
References
- Oniciuc, E.A.; Likotrafiti, E.; Alvarez-Molina, A.; Prieto, M.; Santos, J.A.; Alvarez-Ordóñez, A. The Present and Future of Whole Genome Sequencing (WGS) and Whole Metagenome Sequencing (WMS) for Surveillance of Antimicrobial Resistant Microorganisms and Antimicrobial Resistance Genes across the Food Chain. Genes 2018, 9, 268. [Google Scholar] [CrossRef] [PubMed]
Reference | Microbial Species | Number of Isolates Sequenced | Origin | Main Findings in Relation to AMR |
---|---|---|---|---|
[36] | Aeromonas salmonicida | 101 | Fish | All sequenced isolates harbored three AMR genes against beta-lactam antibiotics encoded on the chromosome. Some isolates also harbored several other plasmid encoded resistance genes against trimethoprim, sulphonamide, and aminoglycoside antibiotics. |
[37] | Campylobacter spp. | 589 | Retail poultry meat | The following AMR genes were identified: tetO, blaOXA-61, aph(2″)-Ic, aph(2″)-If, aph(2″)-Ig, aph(3′)-III, ant(6)-1a, aadE, aph(3″)-VIIa, and Inu(C). Mutations in housekeeping genes (gyrA at position 86, 23S rRNA at position 2074 and 2075) associated with AMR phenotypes were also identified. |
[38] | Campylobacter spp. | 114 | Humans, retail meats, and cecal samples from food production animals | Eighteen resistance genes, including tet(O), blaOXA-61, catA, lnu(C), aph(2″)-Ib, aph(2″)-Ic, aph(2′)-If, aph(2″)-Ig, aph(2″)-Ih, aac(6′)-Ie-aph(2″)-Ia, aac(6′)-Ie-aph(2″)-If, aac(6′)-Im, aadE, sat4, ant(6′), aad9, aph(3′)-Ic, and aph(3′)-IIIa, and mutations in two housekeeping genes (gyrA and 23S rRNA), were identified. |
[26] | Campylobacter coli | 2 | Retail meats | A self-transmissible plasmid carrying multiple antibiotic resistance genes was identified, carrying genes encoding resistance to gentamicin, kanamycin, streptomycin, streptothricin, and tetracycline. Gentamicin resistance was due to a phosphotransferase gene, aph(2″)-Ig, not described previously. |
[39] | Clostridium difficile | 40 | Human and porcine origin | AMR genotypes were characterized by resistance to tetracycline [tetM, tetA(P), tetB(P), and tetW], clindamycin/erythromycin (ermB), and aminoglycosides (aph3-III-Sat4A-ant6-Ia). Resistance was mediated by clinically important mobile genetic elements, most notably Tn6194 (harboring ermB) and a novel variant of Tn5397 (harboring tetM). |
[40] | C. difficile | 2 | Ground pork | Identification of vancomycin (vanW, vanA, vanR, vanS, vex2, vex3, vncR, vncS); fluoroquinolones (gyrA and gyrB); tetracyclines (tetM, translation elongation factor G); beta-lactams (blaZ); and macrolides (macrolide efflux protein, macrolide glycosyltransferase) resistance genes, and multiple multidrug resistance efflux pump genes. |
[31] | Enterococcus spp. | 197 | Various animal and food sources | Resistance genotypes correlated with resistance phenotypes in 96.5% of cases for the 11 drugs investigated. |
[21] | Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Salmonella enterica serovar Typhimurium | 200 | Pigs | High concordance (99.74%) between phenotypic and predicted antimicrobial susceptibility was observed. Correlation between MLST type and resistance profiles was only observed in S. enterica serovar Typhimurium, where isolates belonging to sequence type (ST) 34 were more resistant than ST19 isolates. |
[41] | ESBL-producing Enterobacteriaceae | 24 | Fish and environmental samples | Nine of eleven sequenced fish isolates had the blaCTX-M-15 gene, whereas 12/13 from environment carried blaCTX-M-15. AMR genes encoding resistance to sulfonamides (sul1/sul2), tetracyclines [tet(A)/tet(B)], fluoroquinolones [e.g., aac(6′)-Ib-cr, qnrS1], aminoglycosides [e.g., aac(3)-lld, strB, strA], and trimethoprim (e.g., dfrA14) were detected. |
[42] | E. coli | 17 | Retail chicken meat | All strains carried an IncK plasmid with a blaCMY-2 gene. |
[43] | E. coli | 168 | Broilers and free-range retail poultry (meat/ceca) | The prevalence rates of ESBL producing E. coli among broiler chicken were: meat 46%; ceca 40%. Whereas, those for free range chicken were: meat 15%; ceca 30%. E. coli from broiler and free-range chicken exhibited varied prevalence rates for multi-drug resistance (meat 68%; ceca 64% and meat 8%; ceca 26%, respectively). |
[44] | E. coli | 18 | Dairy cow manure | All sequenced isolates carried at least one β-lactamase bla gene: TEM-1, TEM-81, CTX-M115, CTX-M15, OXA-1, or CMY-2. Several other AMR genes were detected in the sequenced isolates and all of them harbored AMR plasmids belonging to classic Inc groups. |
[45] | E. coli | 16 | Swine farm | blaNDM-5 and mcr-1 were located on two different plasmids, which showed 100% nucleotide identity in all 16 strains. |
[46] | E. coli | 26 | Humans, cows, pigs, horse, rabbit, goat, environments and food | A total of 39 plasmids were identified. Eight plasmids carried resistance genes to aminoglycosides, carbapenems, penicillins, cephalosporins, chloramphenicol, dihydrofolate reductase inhibitors, sulfonamides, tetracyclines, and resistance to heavy metals. Two plasmids carried six of these resistance genes and two novel IncHI2 plasmids were also identified. |
[47] | E. coli | 42 | Feedlot cattle | 70% of the cattle strains carried at least one AMR gene |
[48] | E. coli | 3 | Dairy cows | The mcr-1 gene (linked to colistin resistance) coexisted with multiple resistance genes in a plasmid (pXGE1mcr) |
[49] | E. coli, Salmonella spp. | 463 | Retail meats and farm local samples | To improve the concordance between genotypic and phenotypic data, it was proposed to reduce the phenotypic cut-off values for streptomycin to ≥32 µg mL−1 for both Salmonella and E. coli. |
[50] | Helicobacter pullorum | 4 | Chicken meat | AMR-associated SNPs were detected (linked to resistance to fluoroquinolones, macrolides, and tetracyclines). |
[51] | H. pullorum | 11 | Broiler and free-range chicken | WGS revealed the presence of five or six well characterized AMR genes, including those encoding a resistance-nodulation-division efflux pump |
[30] | Klebsiella pneumoniae | 7 | Pig and human samples at abbatoirs | AMR genes associated with resistance to β-lactams, aminoglycosides, fluoroquinolones, macrolides, lincosamide, streptogramins, rifampicin, sulfonamides, trimethoprim, phenicols, and tetracycline were identified. |
[29] | K. pneumoniae | 44 | Chicken, turkey and pork meat | Meat-source isolates were significantly more likely to be multidrug resistant and resistant to tetracycline and gentamicin than clinical isolates. Four sequence types occurred among both meat-source and clinical isolates. |
[52] | Listeria monocytogenes | 2 | Ready-to-eat food | Seven antibiotic and efflux pump related genes which may confer resistance against lincomycin, erythromycin, fosfomycin, quinolones, tetracycline, penicillin, and macrolides were identified in the genomes of both strains. |
[53] | L. monocytogenes | 5 | Environments from pork processing plants | Strains of a particular sequence type were shown to contain the BAC resistance transposon Tn6188, conveying resistance to quaternary ammonium compounds. |
[54] | Proteus mirabilis | 8 | Food-producing animals | Seven integrative and conjugative elements were identical to ICEPmiJpn1, carrying the cephalosporinase gene blaCMY-2. |
[55] | Non-typhoidal Salmonella | 536 | Retail meat | A total of 65 unique resistance genes, plus mutations in two structural resistance loci, were identified. First finding of extended-spectrum β-lactamases (ESBLs) (blaCTX-M1 and blaSHV2a) in retail meat isolates of Salmonella in the United States. |
[56] | Non-typhoidal Salmonella | 1738 | Animal, food and human sources | The Minimum Inhibitory Concentration (MIC) predictions were correlated with the ResFinder database. The genotypic cut-off values were established for 13 antimicrobials against Salmonella. |
[20] | Non-typhoidal Salmonella | 3491 | Received by Public Health England’s Gastrointestinal Bacteria Reference Unit from different origins for surveillance purposes | Discrepancies between phenotypic and genotypic profiles for one or more antimicrobials were detected for 76 isolates (2.18%). Only 88/52,365 (0.17%) isolate/antimicrobial combinations were discordant. Pan-susceptibility to antimicrobials was observed in 2190 isolates (62.73%). |
[33] | S. enterica | 90 | Dairy cattle and humans | Genotypic prediction of phenotypic resistance resulted in a mean sensitivity of 97.2 and specificity of 85.2. |
[57] | S. enterica serovar Typhimurium | 984 | Swine | Multiple genotypic resistance determinants were predominant, including resistance against ampicillin, streptomycin, sulfonamides, and tetracyclines. Phenotypic resistance to enrofloxacin and ceftiofur was found in conjunction with the presence of plasmid-mediated AMR genes. |
[58] | S. enterica serovar Typhimurium | 1 | Swine carcass | The following AMR genes were identified: tetA, aac3IIa, aadA1, strA, strB, blaTEM-1B, qnrE, sul1, drfA1, and floR. |
[59] | S. enterica serovar Heidelberg | 113 | Humans, abbatoir poultry and retail poultry | CMY-2 plasmids, all belonging to incompatibility group I1, were identified in cefoxitin-resistant isolates. Analysis of IncI1 plasmid sequences revealed high identity (95% to 99%) to a previously described plasmid (pCVM29188_101) found in S. enterica serovar Kentucky. |
[60] | S. enterica serovar Indiana | 1 | Poultry slaughterhouse | 24 multi-drug resistance (MDR) genes, located on 4 plasmids, were identified, including the mcr-1 gene (linked to colistin resistance). |
[61] | S. enterica serovar Infantis | 12 | Humans, food-producing animals and meat | Some isolates harbored a conjugative megaplasmid (~280–320 Kb) which carried the ESBL gene blaCTX-M-1, and additional genes [tet(A), sul1, dfrA1 and dfrA14] mediating cefotaxime, tetracycline, sulfonamide, and trimethoprim resistance. |
[62] | S. enterica serovar Muenster | 2 | Dairy farm environments | The plasmid-mediated qnrB19 gene and IncR plasmid type were identified in both isolates. |
[63] | S. enterica serovar Typhimurium | 225 | Humans, animals, feed, and food | The non-clinical use of narrow-spectrum penicillins (e.g., benzylpenicillin) might have favored the diffusion of plasmids carrying the blaTEM-1 gene in S. enterica serotype Typhimurium in the late 1950s. |
[64] | S. enterica serovar Typhimurium | 4 | Poultry and humans | The following AMR genes were identified: strA, strB, and aadA1 (aminoglycosides); blaTEM-1B (β-lactams); catA1 (phenicols); sul1 and sul2 (sulphonamides); tet B (tetracyclines); and dfrA1 (trimethoprim). |
[65] | S. enterica serovar Typhimurium and S. enterica serovar Kentucky | 2 | Chicken carcasses | A total of five plasmids conveying AMR genes were found. |
[66] | S. enterica serovar Weltevreden | 44 | Human stool and contaminated food samples | AMR genes were only identified in eight isolates, linked to resistance to tetracycline, ciprofloxacin or ampicillin. |
[67] | Staphylococcus aureus | 66 | Retail meats | Eleven spa types were represented. The majority of MRSA (84.8%) possessed SCCmec IV. |
[68] | S. aureus | 9 | Pork, chicken and turkey meat | Multiple resistance genes/mutations were detected. All livestock-associated methicillin-resistant S. aureus (LA-MRSA) harbored tet(M) (±tet(K) and tet(L)), and only seven of these additionally harbored multi-drug resistance to beta-lactams, quinolones, and macrolides. |
[69] | S. aureus | 12 | Livestock animals | Most isolates harbored resistance genes to ≥3 antimicrobial classes in addition to β-lactams. Heavy metal resistance genes were detected in most European ccrC positive isolates, with >80% harboring czrC, encoding zinc and cadmium resistance. |
[70] | S. aureus | 15 | Bulk milk | A divergent mecA homologue (mecALGA251), later named as mecC, was identified. |
[71] | Streptococcus thermophilus | 5 | Raw milk | tet(S) and ermB identified as determinants of AMR. |
[72] | Carbapenem-resistant bacteria | 28 | Dairy cattle | Isolates included: 3 E. coli harboring blaCMY-2 and truncated ompF genes; 8 Aeromonas harboring blacphA-like genes; 1 Acinetobacter baumannii harboring a novel blaOXA gene (blaOXA-497); and 6 Pseudomonas with conserved domains of various carbapenemase-producing genes. |
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Oniciuc, E.A.; Likotrafiti, E.; Alvarez-Molina, A.; Prieto, M.; Santos, J.A.; Alvarez-Ordóñez, A. Correction: Oniciuc, E. A.; et al. The Present and Future of Whole Genome Sequencing (WGS) and Whole Metagenome Sequencing (WMS) for Surveillance of Antimicrobial Resistant Microorganisms and Antimicrobial Resistance Genes across the Food Chain. Genes 2018, 9, 268. Genes 2018, 9, 315. https://doi.org/10.3390/genes9070315
Oniciuc EA, Likotrafiti E, Alvarez-Molina A, Prieto M, Santos JA, Alvarez-Ordóñez A. Correction: Oniciuc, E. A.; et al. The Present and Future of Whole Genome Sequencing (WGS) and Whole Metagenome Sequencing (WMS) for Surveillance of Antimicrobial Resistant Microorganisms and Antimicrobial Resistance Genes across the Food Chain. Genes 2018, 9, 268. Genes. 2018; 9(7):315. https://doi.org/10.3390/genes9070315
Chicago/Turabian StyleOniciuc, Elena A., Eleni Likotrafiti, Adrián Alvarez-Molina, Miguel Prieto, Jesús A. Santos, and Avelino Alvarez-Ordóñez. 2018. "Correction: Oniciuc, E. A.; et al. The Present and Future of Whole Genome Sequencing (WGS) and Whole Metagenome Sequencing (WMS) for Surveillance of Antimicrobial Resistant Microorganisms and Antimicrobial Resistance Genes across the Food Chain. Genes 2018, 9, 268." Genes 9, no. 7: 315. https://doi.org/10.3390/genes9070315
APA StyleOniciuc, E. A., Likotrafiti, E., Alvarez-Molina, A., Prieto, M., Santos, J. A., & Alvarez-Ordóñez, A. (2018). Correction: Oniciuc, E. A.; et al. The Present and Future of Whole Genome Sequencing (WGS) and Whole Metagenome Sequencing (WMS) for Surveillance of Antimicrobial Resistant Microorganisms and Antimicrobial Resistance Genes across the Food Chain. Genes 2018, 9, 268. Genes, 9(7), 315. https://doi.org/10.3390/genes9070315