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Review

A New Perspective on the Health Benefits of Moderate Beer Consumption: Involvement of the Gut Microbiota

by
Mar Quesada-Molina
1,
Araceli Muñoz-Garach
1,2,
Francisco J. Tinahones
1,2,* and
Isabel Moreno-Indias
1,2,*
1
Department of Endocrinology and Nutrition, Virgen de la Victoria Hospital (IBIMA), Malaga University, 29010 Malaga, Spain
2
Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), 29010 Málaga, Spain
*
Authors to whom correspondence should be addressed.
Metabolites 2019, 9(11), 272; https://doi.org/10.3390/metabo9110272
Submission received: 17 September 2019 / Revised: 30 October 2019 / Accepted: 6 November 2019 / Published: 9 November 2019
(This article belongs to the Special Issue Metabolic Health and Weight)
Versions Notes

Abstract

:
Beer is the most widely consumed fermented beverage in the world. A moderate consumption of beer has been related to important healthy outcomes, although the mechanisms have not been fully understood. Beer contains only a few raw ingredients but transformations that occur during the brewing process turn beer into a beverage that is enriched in micronutrients. Beer also contains an important number of phenolic compounds and it could be considered to be a source of dietary polyphenols. On the other hand, gut microbiota is now attracting special attention due to its metabolic effects and as because polyphenols are known to interact with gut microbiota. Among others, ferulic acid, xanthohumol, catechins, epicatechins, proanthocyanidins, quercetin, and rutin are some of the beer polyphenols that have been related to microbiota. However, scarce literature exists about the effects of moderate beer consumption on gut microbiota. In this review, we focus on the relationship between beer polyphenols and gut microbiota, with special emphasis on the health outcomes.

1. Introduction

It has been estimated that approximately 2 billion people worldwide drink alcohol on a daily basis [1]. Although abuse of alcohol is unquestionably harmful, since the proposition of the “French paradox” in the early Nineties [2], possible positive effects of moderate consumption of alcoholic beverages on health have also been extensively investigated. The “French paradox” distinguished between fermented alcoholic beverages and spirits, in several studies the consumption of the former was found to have a positive effect on human health [3,4,5]. However, the debate remains open and a recent study claims that there is no minimum level of alcohol consumption that minimizes health problems [6].
Beneficial effects attributed to a moderate alcohol consumption should not be reduced to a unique single factor, like the amount of alcohol. In fact, most experimental trials have shown differences on health effects between spirits and fermented beverage when similar levels of alcohol consumption are compared [7,8,9], therefore, other additional factors must be considered. Fermented beverages like wine, cider, or beer contain significant amounts of healthy nutrients such as vitamins, polyphenols, or fiber. Wine, particularly red wine, is the fermented beverage that has received most attention due to its putative healthy properties. Moderate wine consumption has been related to a decrease in the risk of cardiovascular disease, type 2 diabetes (through an increase in insulin sensitivity), and favorable plasma lipid profiles [10,11,12]. These results are supported by both epidemiological studies and clinical trials.
However, beer is the most widely consumed fermented beverage in the world and has received less attention than wine. In 2006, nearly 1,700,000,000 hL of beer were drunk in the world [13]. Thus, beer consumption has been attracting increased interest. In this line, several clinical trials have reported that a moderate consumption of beer could mimic some of the previously reported health properties of red wine, among others, mainly due to the antioxidant [3] and anti-inflammatory effects of beer [14], as well as due to a reduced risk of cardiovascular diseases [3,14,15]. Similar to red wine, the mechanisms underlying these positive effects are not fully understood. Thus, in this review, we suggest a role as a mediator factor to the super-organs of gut microbiota.

2. Beer Is a Beverage Rich in Nutrients and Micronutrients

Beer is a fermented beverage produced with a scarce list of raw materials—water, malts (and its adjuncts), hops, and yeast, specifically Saccharomyces cerevisiae. Nevertheless, the technological steps of malting and brewing, and finally storage have important effects on the final composition of beer [16]. Malting is the first step of brewing and consists of mainly the cultivated barley (Hordeum vulgare), or other cereals, through controlled germination. The germinated malt is dried, grinded, and added to hot water in the mashing vessel, to produce a wort containing suitable amounts of fermentable sugars and yeast nutrients, as a result of enzymic hydrolysis or dissolving substances. This wort is boiled and mixed with hops (Humulus lupulus), a member of the Cannabinaceae family. In this step, α-acids humulones are transformed into trans-isohumulones, which provides the characteristic bitterness of beer. After this, a separation step is performed in order to remove the plant residues. At this moment, fermentation is initiated with the yeast strain and alcohol and carbon dioxide is produced. Finally, the maturation step during storage allows the formation of the particular aroma of each class of beer [17].
Thus, when the final product is ready, it contains hundreds of different compounds, some derived from raw materials that pass unchanged through brewing, while others are produced as a result of the technological process or during their maturation phase [18].
In beer, water represents more than 90% of its composition. Carbohydrates are the major nonvolatile component in beer with 3.3–4.4%, which comprise mainly dextrins (75–80%), monosaccharides, and oligosaccharides (20–30%), and pentosans (5–8%). Fermentation leads to the production of ethanol and a series of by-products, including other alcohols, carbonyl compounds, esters, aldehydes, and acids. The final alcohol content usually ranges from 1.0% to 6.0%, depending on the type of beer. The characteristic bitterness of a finished beer is derived from hops, especially from α-acids (humulones) and β-acids (lupulones). Iso-α-acids represent a varied concentration from 15 mg/L in typical American lagers to almost 100 mg/L in very bitter English ales [19]. In general, organic acids belong to yeast and bacterial fermentation; while the inorganic compounds found in beer are metal cations, trace metals, and anions, which influence the drink’s clarity and salty taste. The presence of these compounds is related to the initial raw materials, the brewing process or the packaging of the final product. Concentrations of inorganic components generally range from 0.5 to 2 g/L (for more information [16]).
Thus, beer is especially interesting because of its wide range of micronutrients. Beer includes a relatively significant content of folate (2.2–24.2 µg per bottle [20]) and choline (9.71 mg/100 mL [21]). Beer also contains trace amounts of minerals, such as calcium, iron, magnesium, phosphorus, potassium, sodium, zinc, copper, manganese, selenium, fluoride, and silicon [22].
Nevertheless, other components in beer are receiving increased attention because of their bioactive properties. This is the case of polyphenols, which confer special features to this beverage. Beer contains several polyphenols which are mainly derived from malt and hops [22].
These components have historically been associated with technological quality damage. However, their nutritional characteristics are being currently revised due to the interest in their potential health benefits [23].

3. Polyphenols and Health Benefits

Polyphenols are chemical and biologically active plant secondary metabolites derived from phenylalanine and tyrosine. This family of molecules is characterized by the presence of phenol (hydroxybenzene) units in their chemical structure. Plants synthesize these molecules as a response to several stress situations. These phytochemicals are ingested in food such as fruits, vegetables, and cereals. High levels of polyphenols are found in tea, coffee, wine, beer, or extra-virgin olive oil [24]. Some studies have attempted to link the observed health effects of a diet rich in fruits and vegetables with the content of polyphenols in these edible products [25].
Polyphenols display a wide range of biological activities that has been assessed in many studies, mainly in vitro studies with cell lines essays, but also with animal trials. Several advantageous effects have been reported, including decreases in markers of inflammation like IFM-γ, IL-1β, or NF-κB [26], and inhibitions of the expression of adhesion molecules, such as VCAM-1 and ICAM-1 [27]. Reduction of oxidative stress by polyphenols has also been demonstrated and has been related to a lower activity of cytochrome P450 [28] or through modification of gene expression via intracellular signaling cascades by, for example, reducing NF-κB or enhancing nuclear factor-like 2 (Nrf2) [29]. Furthermore, it has been reported that dietary phytochemicals improve anticancer markers [30], and might result in epigenetic modifications which confer protection against cancer [31]. Lastly, some trials have shown that some specific phenolic structures might have anti-diabetic [32] and antimicrobial properties [33]. Additionally, many articles have been written on polyphenols derived from hops and their properties during the last few years [34].
All these reported activities seem to be related and promoted by a synergistic effect of different polyphenols [10]. Particularly, polyphenols present in beer have been shown to exert a protective effect on the incidence of cardiovascular events, after moderate beer consumption [3]. The effects of alcohol and polyphenols from beer on atherosclerotic biomarkers have also been analyzed, and the results show that the phenolic content of beer reduces leukocyte adhesion molecules and inflammatory biomarkers [14]. Additionally, this polyphenolic content seems to elevate the number of circulating endothelial progenitor cells (EPC), a surrogate marker of vascular function and cumulative cardiovascular risk, after 4 weeks of beer consumption [7]. Conversely, alcohol mainly improves the lipid profile and reduces some plasma inflammatory biomarkers related to atherosclerosis [3,14].
Particularly, some specific beer-derived polyphenols exert interesting biological responses. They include anti-diabetic [8,35], anti-carcinogenic [36,37,38,39], and anti-inflammatory effects [40]. These specific polyphenols could be potential drugs used to treat related diseases. However, many of the mechanisms underlying these properties have not yet been elucidated. One hypothesis is that, at least partially, gut microbiota could mediate some of these beneficial effects. In this line, although not specially focused on beer polyphenols, several studies have been recently published emphasizing the role of gut microbiota for the bioavailability and physiological functions of dietary polyphenols [41,42].

4. Beer Is a Fermented Beverage with a Wide Range of Polyphenols

Absolute polyphenols content in beer is not higher than in other dietary products. According to the phenol explorer database, the total polyphenol content of beer ranges 12 to 52 mg/100 mL, which is in the same range as that of white wine (32 mg/100 mL), but is much lower than that in red wine (216 mg/100 mL) [43]. However, considering that the average beer intake is much higher than the consumption of red wine [10], relative contribution of beer to the daily polyphenol intake is probably the highest among fermented beverages.
Additionally, polyphenols refer to a wide family of biological compounds and its content level are only one of the variables to be considered. Even more important is the chemical diversity present in alcoholic beverages. Table 1 shows a classification of the polyphenols found in beer. Beer polyphenols are derived from malt (two-thirds) and hops (one-third) [18]. Polyphenols content depends on different factors, mainly crop variety [22] and environmental conditions of cultivation [25], as well as the technological brewing process followed [44].
Concerning bioactivity, beer polyphenols, especially those derived from hops, such as xanthohumol and isoxanthohumol, have a great potential [34]. The scientific literature has been mainly focused on the antioxidant activity of polyphenols for human health; however, polyphenols could also have other important properties that require more research.

5. Gut Microbiota and Polyphenols

The human intestine is inhabited by bacteria, archaea, fungi, protozoa, and viruses [46], which interact in a complex and dynamic symbiosis with the host. Around 1014 bacteria of more than 1000 different species has been identified in humans [47]. Gut microbiota survival is mainly maintained through the host diet, especially through the host-derived chemical compounds and metabolic byproducts excreted by the microbes themselves. Indeed, cooperation among microbial members, but also their competition for resources, are essential for the maintenance of the population within the gut environment [48]. However, the interactions of the gut microbiota with the host affect many aspects of human health and disease [49,50]. A complex network of different intestinal microbiota members is necessary for nutrient absorption in general and for the full polyphenols’ biotransformation, in particular [41].
Polyphenols are usually present and stored in plant tissues in the form of diverse derivatives, mostly as sugar O-conjugates [25], which confer extra structural stability [51]. Moreover, simple phenolics and their derivatives are usually covalently linked to polysaccharides in the plant cell wall [33]. Conjugation increases the chemical stability of polyphenols and allows high concentrations of these conjugated molecules in food and beverages. Nevertheless, their degree of structural complexity and polymerization will determine their absorption and bioavailability for the host [52,53]. Low molecular-weight polyphenols could be readily absorbed in the small intestine, while oligomeric and polymeric polyphenols will reach the colon with minimal chemical modifications [54]. Most dietary polyphenols belong to this last group and reach the colon. These complex polyphenols get in contact with the intestinal microbiota and are finally transformed. This conversion is often essential for absorption and bioavailability, modulating the biological activity of these dietary compounds [52,53]. These absorbed compounds enter the portal vein circulation towards final transformation in the liver, where other enzymatic activities are present. A complex network of different intestinal microbiota species is involved in this biochemical polyphenols processing [33]. To understand this gut microbiota–polyphenols interaction, it is essential to solve the mechanism of polyphenol bioactivity. It is a complex equation to solve because it is a two-way interaction and gut microbiota is also affected by polyphenolic compounds. These polyphenolic compounds and their metabolites, some produced by their own microbiota, are able to modulate the gut microbial community, mostly through the inhibition of pathogenic bacteria and the stimulation of beneficial bacteria [23,55]. Thus, gut microbiota bio-transforms polyphenols into their metabolites, resulting in a greater bioavailability of polyphenols, which, at once, are able to modulate the gut microbiota community mostly through the inhibition of pathogenic bacteria and the stimulation of beneficial members. In this last example, polyphenols might act as prebiotic metabolites which enrich the beneficial bacteria [23,55].

6. Interaction between Polyphenols Present in Fermented Alcoholic Beverages and Gut Microbiota

Toxicity of alcohol is involved in generating health complications. Conversely, alcohol is able to improve the lipid profile and reduce plasma inflammatory biomarkers related to atherosclerosis [3,14]. Alcohol might also produce an alteration of microbiota composition or function [47]. In a trial with chronic consumption of gin, an alcoholic beverage without any polyphenols, an increase in the abundances of Clostridium and Clostridium hystoliticum group was observed [9]. However, not all intake of alcoholic beverages yielded similar results. Other changes in the gut microbiota have been reported after consuming fermented or distilled beverages [9]. In polyphenol-rich alcoholic beverages such as red wine, chronic consumption resulted in an increased population of Enterococcus, Prevotella, Bacteroides, Bifidobacterium, Bacteroides uniformis, Eggerthella lenta, and Blautia coccoides–Eubacterium rectale groups [9]. Moreno-Indias et al. (2016) also observed a significant increase in the protectors of the gut mucosal barrier [5], such as Bifidobacterium spp. and Lactobacillus spp. This study was performed with regular red wine and de-alcoholized red wine intake periods by patients suffering from obese metabolic syndrome. Bifidobacterium spp. and Lactobacillus spp. are able to degrade phenolic compounds such as anthocyanin metabolites [56]. Additionally, Lactobacillus strains might advocate the normalization of the gut microbiota community and mitigate inflammation. Members of Lactobacillus are also able to reduce intestinal permeability, to improve the immunological functions of the intestine and to mitigate the intestinal inflammatory response as consequence [57]. On the other hand, Bifidobacterium has the capacity to produce lactate and acetate, as well as the ability to inhibit the growth of pathogenic bacteria [58]. Moreover, Bifidobacterium has been previously associated with the decrease of plasma cholesterol levels [59,60]. In this last study, independent to ethanol, wine polyphenols ingestion affected the growth of butyrate producers, namely the Blautia coccoides–Eubacterium rectale group, Faecalibacterium prausnitzii, and Roseburia. Additionally, this study showed that red wine polyphenols reduced the growth of the Clostridium histolyticum group, which includes important pathogens that are closely related to the progression of colon cancer and the onset of inflammatory bowel disease [61]. These results agree with those obtained by Tzounis et al., which make the essay with cocoa flavan-3-ols instead of wine [62].

7. Beer Polyphenols and Its Relationship with Gut Microbiota

As in other fermented beverages like wine, beer composition includes an important percentage of phenolic compounds. Table 2 summarizes some of the interactions between beer polyphenols and gut microbiota reported in the scientific literature. The most abundant hydroxycinnamic acid found in beer is ferulic acid, which mainly originates from barley [63]. However, many of the most interesting beer polyphenols belong to hops. Dried hops contain 4–14% polyphenols, mainly phenolic acids, prenylated chalcones, flavonoids, catechins, epicatechins, and proanthocyanidins [64]. Xanthohumol and its derivates mainly appear during brewing and particularly during the boiling of the wort. Isoxanthohumol, 8-prenylnaringenin, and 6- prenylnaringenin are the molecules most extensively studied and they have positively tested as displaying anti-obesity [65], anti-carcinogenic [66], and antidiabetic [67,68] activities. The bioactivity of these molecules is usually correlated with their amount, although in the case of xanthohumol derivatives, these metabolites exert a great activity at low concentrations [69,70,71].
The number of studies assessing the effects of moderate consumption of beer on the gut microbiota is low when compared with the number of clinical trials, epidemiological studies, and in vitro essays that show positive effects of this intake on human health. However, derivatives from hop polyphenols, such as isoxanthohumol, have been studied in relation to gut microbiota. In fact, isoxanthohumol is considered an accurate biomarker of beer consumption [86]. This molecule can be metabolized by gut microbiota to render 8-prenylnaringenin [72,73] through an O-demethylation that is carried out by Eubacterium species and particularly by Eubacterium limosum or Eubacterium ramulus that is responsible for this transformation [75]. The genus Eubacterium is one of the biggest butyric acid producers [87] and deserves further investigation. However, little attention has been paid to other hops compounds such as alpha- and beta-acids and their interaction with gut microbiota. Recently, Blatchford et al. (2019) used a pH-controlled anaerobic batch fermenter with a human fecal inoculum to investigate the interaction of a supercritical CO2 extract of hops on the human gut microbiota population [76]. Interestingly, they reported a decrease in butyrate concentrations aligned with a reduction of butyrate producers like Eubacterium and Coprococcus, as well as Bifidobacterium. Moreover, they found an increase of Enterobacteriaceae and Akkermansia [76].
Other studies have focused on ferulic acid, which is the most abundant polyphenol in beer with a concentration of 6.5 mg/L [10]. Six bacterial isolates from human feces have been reported to be able to release ferulic acid from its ethyl ester in vitro: Escherichia coli (three isolates), Bifidobacterium lactis, and Lactobacillus gasseri (two strains) [77]. On the other hand, rats in vivo essays showed that ferulic acid was related to an increase in bacterial richness and diversity [78,79]. This molecule is part of dietary fiber, the fundamental indigestible portion of food for the development of gut microbiota and especially for those members related to beneficial outcomes. Ferulic acid supplementation is able to modulate the ratio of Firmicutes to Bacteroidetes, as well as the production of indole-3-acetic acid in a nonalcoholic fatty liver disease model of mouse [80].
Some flavan-3-ols, such as catechins and epicatechins, are also found at high concentrations in beer (around 5.4 and 3.3 mg/L, respectively [10]). Eggerthella lenta and Flavonifractor plautii are able to biotransform dietary catechins into valerolactones and hydroxyvaleric acid metabolites [64]. Among the bioactive effects reported, it has been shown that epicatechin, catechin, 3-O-methylgallic acid, gallic acid, and caffeic acid are able to repress Clostridium perfringens, Clostridium difficile, and Bacteroides spp. growth, while other anaerobes commensal, such as Clostridium spp., Bifidobacterium spp. or Lactobacillus spp. were less negatively affected [55]. Flavonifractor plautii is also able to convert B type proanthocyanidins into valerolactones [88]. Conversely, proanthocyanidins extracts in humans, promoted the growth of Akkermansia muciniphila and improved diet-induced obesity, insulin resistance, and metabolic syndrome [89].
Finally, other flavonols present in beer are quercetin and rutin. Butyrivibrio spp. from ruminal fluid has the ability to cleave the C ring of rutin. In contrast, quercetin is cleaved by Eubacterium oxidoreducens, which was recovered from the bovine rumen [83]. Likewise, isolates of Clostridium orbiscindens strains from humans were found to be capable of cleaving the C ring of quercetin at the bond between the 3- and 4-positions [90].

8. Beer Polyphenols, Relationship with Gut Microbiota, and Health Benefits

The interaction among polyphenols present in beer and gut microbiota has recently been reported to have important health benefits, when changes in gut microbiota was analyzed after a moderate consumption of beer. Hernández-Quiroz et al. (2019) have recently reported a proliferation of Bacteroidetes with respect to Firmicutes, after a month of consuming an alcoholic or non-alcoholic beer on a daily basis [91]. These authors registered a higher diversity after the non-alcoholic beer, something that was translated into a decrease in fasting blood serum glucose and an increase in functional β cells. These results were not observed after the consumption of the alcoholic beer. Although it was not directly measured, it was concluded by the authors that health improvements were related to the biologically active polyphenol and phenolic acids [91].
Thus, to investigate the role of the polyphenols present in beer on health outcomes, through their gut–microbiota interactions, requires systemic approaches; meanwhile this action needs to be inferred. It has been reported that metabolites derived from hop have a positive effect on different health variables and they could also affect the gut microbiota populations. In this line, a study in mice found that tetra-hidro iso-alpha acids from hop, apart from reducing body weight gain, were able to reduce the fat mass, to alleviate glucose intolerance and to normalize insulin sensitivity markers in obese and diabetic mice [40]. These promising results were also related to gut microbiota, particularly to a reduction in plasma lipopolyshaccaride (LPS) levels and gut permeability, as well as higher intestinal tight junction proteins (Zonula occludens-1 and occludin), anti-inflammatory cytokine interleukin-10, and a decrease in pro-inflammatory cytokine granulocyte colony-stimulating factor [40]. Additionally, a recent study in DIO mice have revealed that chronic iso-alpha acid treatment altered enteroendocrine hormone levels and bile acid homeostasis and stimulated sustained GLP-1 release. As a result, this chronic treatment generated mass and weight loss, increased the energy expenditure, improved glucose tolerance and insulin sensitivity, and recovered the plasma lipids and inflammatory markers [67]. Many other studies have been developed with polyphenols from hops and their derivates. Two recent publications revealed that xanthohumol and 8-prenylnaringenin could ameliorate diabetic-associated metabolic disturbances by regulating glucose and lipid pathways [8,92]. On the other hand, several in vitro studies with human cancer cell lines have evidenced an anticancer activity, in both xanthohumol and 8-prenylnaringenin, with a decreased proliferation and invasion in colon [92], breast [38], prostate [93], and ovarian [38] cancers. Furthermore, a recent article has shown that the antiproliferative activity against human cancer cell lines of these molecules is very selective and dependent on the prenyl group, with respect to antiproliferative activity, as naringenin exerted a low activity [69]. However, beyond the fact that gut microbiota activates these phytoestrogens [72], no other relationships have been established between xanthohumol, gut microbiota, and cancer. It would be interesting to verify the relationship with the gut microbiota in order to decipher mechanisms subjacent to these actions.
Furthermore, other polyphenols present in beer has been related positively with health. The flavonol quercetin counteracted gut microbiota dysbiosis in rats fed with a high-fat sucrose diet and it also prevented body-weight gain, reduced serum insulin, attenuated the Firmicutes/Bacteroidetes ratio, and inhibited the growth of bacteria associated with diet-induced obesity [88]. Conversely, tannins like catechin, epicatechin, and proanthocyanidin have been reported to attenuate the overexpression of NF-κB, AMPK, TGF-β, PARP, and IL-6, factors which are involved in the progression of diabetic complications [94].

9. Conclusions

The available scientific literature reviewed in this manuscript supports that a complex interaction between polyphenols and gut microbiota could play a significant role on the healthy benefits that moderate beer consumption seems to provide. However, extensive experimental research is needed to confirm this hypothesis and to elucidate more details of this interaction. In fact, along this line, the recently available study by Hernández-Quiroz et al. (2019) has proposed this hypothesis to discuss their results [91]. Until now, beer is considered to be a popular refreshing beverage, but little attention has been given to the role that its bioactive components could exert on health, mainly the diverse polyphenols components. This review and other research reports showed that further investigations are needed, focusing on the causes of the health implications associated to xanthohumol and other derivatives from hop polyphenols, since the published results seems very promising. More research is also needed on the role of the more abundant polyphenols present in the beer, such as ferulic acid, to get a better knowledge on their interaction with gut microbiota.

Author Contributions

Writing—Original Draft preparation M.Q.-M.; contextualization A.M.-G.; supervision F.J.T. and I.M.-I.; funding acquisition F.J.T. and I.M.-I.

Funding

M.Q.-M. was supported by a Manuel de Oya Research fellowship from the Centro de Información Cerveza y Salud. I.M.-I. was supported by the “MS type I” program (CP16/00163) and A.M.-G. by a “Juan Rodes” postdoctoral contract (JR17/00023), from the Instituto de Salud Carlos III cofounded by Fondo Europeo de Desarrollo Regional–FEDER.

Acknowledgments

We acknowledge the support of “Centro de Información Cerveza y Salud” and The Metagenomic platform CIBER-IBIMA. Authors thank MA Quesada for his intense language support in the manuscript. Moreover, the authors would like to thank the anonymous reviewers for their helpful and constructive comments that greatly contributed to improving the final version of the paper.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

  1. World Health Organization. Global Status Report on Alcohol 2004; WHO: Geneva, Switzerland, 2004. [Google Scholar]
  2. Renaud, S.; De Lorgeril, M. Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet 1992, 339, 1523–1526. [Google Scholar] [CrossRef]
  3. De Gaetano, G.; Costanzo, S.; Di Castelnuovo, A.; Badimon, L.; Bejko, D.; Alkerwi, A.; Chiva-Blanch, G.; Estruch, R.; La Vecchia, C.; Panico, S.; et al. Effects of moderate beer consumption on health and disease: A consensus document. Nutr. Metab. Cardiovasc. Dis. 2016, 26, 443–467. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Di Castelnuovo, A.; Costanzo, S.; Bagnardi, V.; Donati, M.B.; Iacoviello, L.; de Gaetano, G. Alcohol dosing and total mortality in men and women: An updated meta-analysis of 34 prospective studies. Arch. Intern. Med. 2006, 166, 2437–2445. [Google Scholar] [CrossRef] [PubMed]
  5. Moreno-Indias, I.; Sánchez-Alcoholado, L.; Pérez-Martínez, P.; Andrés-Lacueva, C.; Cardona, F.; Tinahones, F.; Queipo-Ortuño, M.I. Red wine polyphenols modulate fecal microbiota and reduce markers of the metabolic syndrome in obese patients. Food Funct. 2016, 7, 1775–1787. [Google Scholar] [CrossRef] [PubMed]
  6. GBD 2016 Alcohol Collaborators. Alcohol use and burden for 195 countries and territories, 1990–2016: A systematic analysis for the Global Burden of Disease Study 2016. Lancet 2018, 392, 1015–1035. [Google Scholar] [CrossRef]
  7. Chiva-Blanch, G.; Condines, X.; Magraner, E.; Roth, I.; Valderas-Martínez, P.; Arranz, S.; Casas, R.; Martínez-Huélamo, M.; Vallverdú-Queralt, A.; Rada, P.Q.; et al. The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial. Atherosclerosis 2014, 233, 518–524. [Google Scholar] [CrossRef] [PubMed]
  8. Costa, R.; Rodrigues, I.S.O.P.E.F.; Guardão, L.; Rocha-Rodrigues, S.; Silva, C.; Magalhães, J.; Ferreira-De-Almeida, M.; Negrão, R.; Soares, R. Xanthohumol and 8-prenylnaringenin ameliorate diabetic-related metabolic dysfunctions in mice. J. Nutr. Biochem. 2017, 45, 39–47. [Google Scholar] [CrossRef]
  9. Murri, M.; Gómez-Zumaquero, J.M.; Clemente-Postigo, M.; Estruch, R.; Diaz, F.C.; Andres-Lacueva, C.; Tinahones, F.J.; Queipo-Ortuño, M.I.; Boto-Ordóñez, M. Influence of red wine polyphenols and ethanol on the gut microbiota ecology and biochemical biomarkers. Am. J. Clin. Nutr. 2012, 95, 1323–1334. [Google Scholar]
  10. Arranz, S.; Chiva-Blanch, G.; Valderas-Martínez, P.; Medina-Remón, A.; Lamuela-Raventos, R.M.; Estruch, R. Wine, Beer, Alcohol and Polyphenols on Cardiovascular Disease and Cancer. Nutrients 2012, 4, 759–781. [Google Scholar] [CrossRef] [Green Version]
  11. Hodgson, J.M.; Croft, K.D. Dietary flavonoids: Effects on endothelial function and blood pressure. J. Sci. Food Agric. 2006, 86, 2492–2498. [Google Scholar] [CrossRef]
  12. Roberfroid, M.; Gibson, G.R.; Hoyles, L.; McCartney, A.L.; Rastall, R.; Rowland, I.; Wolvers, D.; Watzl, B.; Szajewska, H.; Stahl, B.; et al. Prebiotic effects: Metabolic and health benefits. Br. J. Nutr. 2010, 104, S1–S63. [Google Scholar] [CrossRef] [PubMed]
  13. Buiatti, S. Beer Composition: An Overview. In Beer in Health and Disease Prevention; Preedy, V.R., Ed.; Academic Press: San Diego, CA, USA, 2009; pp. 213–225. [Google Scholar] [CrossRef]
  14. Chiva-Blanch, G.; Magraner, E.; Condines, X.; Valderas-Martinez, P.; Roth, I.; Arranz, S.; Casas, R.; Navarro, M.; Hervas, A.; Siso, A.; et al. Effects of alcohol and polyphenols from beer on atherosclerotic biomarkers in high cardiovascular risk men: A randomized feeding trial. Nutr. Metab. Cardiovasc. Dis. 2015, 25, 36–45. [Google Scholar] [CrossRef] [PubMed]
  15. Martinez, N.; Urpi-Sarda, M.; Martinez-Gonzalez, M.A.; Andres-Lacueva, C.; Mitjavila, M.T. Dealcoholised beers reduce atherosclerosis and expression of adhesion molecules in apoE-deficient mice. Br. J. Nutr. 2011, 105, 721–730. [Google Scholar] [CrossRef] [PubMed]
  16. Anderson, H.E.; Santos, I.C.; Hildenbrand, Z.L.; Schug, K.A. A review of the analytical methods used for beer ingredient and finished product analysis and quality control. Anal. Chim. Acta 2019, 1085, 1–20. [Google Scholar] [CrossRef] [PubMed]
  17. Humia, B.V.; Santos, K.S.; Barbosa, A.M.; Sawata, M.; Mendonça, M.D.C.; Padilha, F.F. Beer Molecules and Its Sensory and Biological Properties: A Review. Molecules 2019, 24, 1568. [Google Scholar] [CrossRef]
  18. Beer in Health and Disease Prevention; Elsevier: Amsterdam, The Netherlands, 2009.
  19. De Keukeleire, D. Fundamentals of beer and hop chemistry. Quim. Nova 2000, 23, 108–112. [Google Scholar] [CrossRef]
  20. Owens, J.E.; Clifford, A.J.; Bamforth, C.W. Folate in Beer. J. Inst. Brew. 2007, 113, 243–248. [Google Scholar] [CrossRef]
  21. Zeisel, S.H.; Mar, M.-H.; Howe, J.C.; Holden, J.M. Concentrations of choline-containing compounds and betaine in common foods. J. Nutr. 2003, 133, 1302–1307. [Google Scholar] [CrossRef]
  22. United States Department of Agriculture. USDA Food Composition Databases. Available online: https://ndb.nal.usda.gov/ndb/search/list (accessed on 14 November 2018).
  23. Ozdal, T.; Sela, D.A.; Xiao, J.; Boyacioglu, D.; Chen, F.; Capanoglu, E. The Reciprocal Interactions between Polyphenols and Gut Microbiota and Effects on Bioaccessibility. Nutrients 2016, 8, 78. [Google Scholar] [CrossRef]
  24. Puupponen-Pimiä, R.; Aura, A.-M.; Oksman-Caldentey, K.-M.; Myllärinen, P.; Saarela, M.; Mattila-Sandholm, T.; Poutanen, K. Development of functional ingredients for gut health. Trends Food Sci. Technol. 2002, 13, 3–11. [Google Scholar] [CrossRef]
  25. Bohn, T. Dietary factors affecting polyphenol bioavailability. Nutr. Rev. 2014, 72, 429–452. [Google Scholar] [CrossRef] [PubMed]
  26. Magni, G.; Marinelli, A.; Riccio, D.; Lecca, D.; Tonelli, C.; Abbracchio, M.P.; Petroni, K.; Ceruti, S. Purple Corn Extract as Anti-allodynic Treatment for Trigeminal Pain: Role of Microglia. Front. Cell. Neurosci. 2018, 12, 378. [Google Scholar] [CrossRef] [PubMed]
  27. Martinez, N.; Casos, K.; Simonetti, P.; Saiz, M.P.; Moreno, J.J.; Mitjavila, M.T. De-alcoholised white and red wines decrease inflammatory markers and NF-kappaB in atheroma plaques in apoE-deficient mice. Eur. J. Nutr. 2013, 52, 737–747. [Google Scholar] [CrossRef] [PubMed]
  28. Fogliano, V.; Corollaro, M.L.; Vitaglione, P.; Napolitano, A.; Ferracane, R.; Travaglia, F.; Arlorio, M.; Costabile, A.; Klinder, A.; Gibson, G. In vitro bioaccessibility and gut biotransformation of polyphenols present in the water-insoluble cocoa fraction. Mol. Nutr. Food Res. 2011, 55, S44–S55. [Google Scholar] [CrossRef] [PubMed]
  29. Virgili, F.; Marino, M. Regulation of cellular signals from nutritional molecules: A specific role for phytochemicals, beyond antioxidant activity. Free Radic. Boil. Med. 2008, 45, 1205–1216. [Google Scholar] [CrossRef]
  30. Mabrok, H.B.; Klopfleisch, R.; Ghanem, K.Z.; Clavel, T.; Blaut, M.; Loh, G. Lignan transformation by gut bacteria lowers tumor burden in a gnotobiotic rat model of breast cancer. Carcinogenesis 2012, 33, 203–208. [Google Scholar] [CrossRef] [PubMed]
  31. Shankar, E.; Kanwal, R.; Candamo, M.; Gupta, S. Dietary phytochemicals as epigenetic modifiers in cancer: Promise and challenges. Semin. Cancer Boil. 2016, 40–41, 82–99. [Google Scholar] [CrossRef]
  32. Spínola, V.; Pinto, J.; Llorent-Martínez, E.J.; Tomás, H.; Castilho, P.C. Evaluation of Rubus grandifolius L. (wild blackberries) activities targeting management of type-2 diabetes and obesity using in vitro models. Food Chem. Toxicol. 2019, 123, 443–452. [Google Scholar] [CrossRef]
  33. Marín, L.; Miguélez, E.M.; Villar, C.J.; Lombó, F. Bioavailability of Dietary Polyphenols and Gut Microbiota Metabolism: Antimicrobial Properties. BioMed Res. Int. 2015, 2015, 1–18. [Google Scholar] [CrossRef] [Green Version]
  34. Lin, M.; Xiang, D.; Chen, X.; Huo, H. Role of Characteristic Components of Humulus lupulus in Promoting Human Health. J. Agric. Food Chem. 2019, 67, 8291–8302. [Google Scholar] [CrossRef]
  35. Miranda, C.L.; Elias, V.D.; Hay, J.J.; Choi, J.; Reed, R.L.; Stevens, J.F. Xanthohumol improves dysfunctional glucose and lipid metabolism in diet-induced obese C57BL/6J mice. Arch. Biochem. Biophys. 2016, 599, 22–30. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Aichinger, G.; Beisl, J.; Marko, D. The Hop Polyphenols Xanthohumol and 8-Prenyl-Naringenin Antagonize the Estrogenic Effects of Fusarium Mycotoxins in Human Endometrial Cancer Cells. Front. Nutr. 2018, 5. [Google Scholar] [CrossRef] [PubMed]
  37. Jiang, C.-H.; Sun, T.-L.; Xiang, D.-X.; Wei, S.-S.; Li, W.-Q. Anticancer Activity and Mechanism of Xanthohumol: A Prenylated Flavonoid from Hops (Humulus lupulus L.). Front. Pharmacol. 2018, 9, 530. [Google Scholar] [CrossRef] [PubMed]
  38. Miranda, C.; Stevens, J.; Helmrich, A.; Henderson, M.; Rodriguez, R.; Yang, Y.-H.; Deinzer, M.; Barnes, D.; Buhler, D. Antiproliferative and cytotoxic effects of prenylated flavonoids from hops (Humulus lupulus) in human cancer cell lines. Food Chem. Toxicol. 1999, 37, 271–285. [Google Scholar] [CrossRef]
  39. Stevens, J.F.; Page, J.E. Xanthohumol and related prenylflavonoids from hops and beer: To your good health! Phytochemistry 2004, 65, 1317–1330. [Google Scholar] [CrossRef]
  40. Everard, A.; Geurts, L.; Van Roye, M.; Delzenne, N.M.; Cani, P.D. Tetrahydro iso-Alpha Acids from Hops Improve Glucose Homeostasis and Reduce Body Weight Gain and Metabolic Endotoxemia in High-Fat Diet-Fed Mice. PLoS ONE 2012, 7, e33858. [Google Scholar] [CrossRef]
  41. Kawabata, K.; Yoshioka, Y.; Terao, J. Role of Intestinal Microbiota in the Bioavailability and Physiological Functions of Dietary Polyphenols. Molecules 2019, 24, 370. [Google Scholar] [CrossRef]
  42. Singh, A.K.; Cabral, C.; Kumar, R.; Ganguly, R.; Rana, H.K.; Gupta, A.; Lauro, M.R.; Carbone, C.; Reis, F.; Pandey, A.K. Beneficial Effects of Dietary Polyphenols on Gut Microbiota and Strategies to Improve Delivery Efficiency. Nutrients 2019, 11, 2216. [Google Scholar] [CrossRef]
  43. Database on Polyphenol Content in Foods. Available online: http://phenol-explorer.eu/reports/38#wine%20text (accessed on 17 October 2019).
  44. Bu, S.Y.; Kim, M.H.; Choi, M.K. Effect of Silicon Supplementation on Bone Status in Ovariectomized Rats under Calcium-Replete Condition. Biol. Trace Elem. Res. 2016, 171, 138–144. [Google Scholar] [CrossRef]
  45. Gerhauser, C. Beer constituents as potential cancer chemopreventive agents. Eur. J. Cancer 2005, 41, 1941–1954. [Google Scholar] [CrossRef]
  46. Barko, P.C.; McMichael, M.A.; Swanson, K.S.; Williams, D.A. The Gastrointestinal Microbiome: A Review. J. Vet. Intern. Med. 2018, 32, 9–25. [Google Scholar] [CrossRef] [PubMed]
  47. Engen, P.A.; Green, S.J.; Voigt, R.M.; Forsyth, C.B.; Keshavarzian, A. The Gastrointestinal Microbiome: Alcohol Effects on the Composition of Intestinal Microbiota. Alcohol Res. Curr. Rev. 2015, 37, 223–236. [Google Scholar]
  48. Sung, J.; Kim, S.; Cabatbat, J.J.T.; Jang, S.; Jin, Y.-S.; Jung, G.Y.; Chia, N.; Kim, P.-J. Global metabolic interaction network of the human gut microbiota for context-specific community-scale analysis. Nat. Commun. 2017, 8, 15393. [Google Scholar] [CrossRef] [Green Version]
  49. Qin, J.; Li, R.; Raes, J.; Arumugam, M.; Burgdorf, K.S.; Manichanh, C.; Nielsen, T.; Pons, N.; Levenez, F.; Yamada, T.; et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 2010, 464, 59–65. [Google Scholar] [CrossRef] [Green Version]
  50. Turnbaugh, P.J.; Ley, R.E.; Hamady, M.; Fraser-Liggett, C.M.; Knight, R.; Gordon, J.I. The human microbiome project. Nature 2007, 449, 804–810. [Google Scholar] [CrossRef] [PubMed]
  51. Marinova, K.; Kleinschmidt, K.; Weissenböck, G.; Klein, M. Flavonoid Biosynthesis in Barley Primary Leaves Requires the Presence of the Vacuole and Controls the Activity of Vacuolar Flavonoid Transport. Plant Physiol. 2007, 144, 432–444. [Google Scholar] [CrossRef] [Green Version]
  52. Hollman, P.C.; Bijsman, M.N.; Van Gameren, Y.; Cnossen, E.P.; De Vries, J.H.; Katan, M.B. The sugar moiety is a major determinant of the absorption of dietary flavonoid glycosides in man. Free Radic. Res. 1999, 31, 569–573. [Google Scholar] [CrossRef]
  53. Lewandowska, U.; Szewczyk, K.; Hrabec, E.; Janecka, A.; Gorlach, S. Overview of Metabolism and Bioavailability Enhancement of Polyphenols. J. Agric. Food Chem. 2013, 61, 12183–12199. [Google Scholar] [CrossRef]
  54. Cardona, F.; Andres-Lacueva, C.; Tulipani, S.; Tinahones, F.J.; Queipo-Ortuño, M.I. Benefits of polyphenols on gut microbiota and implications in human health. J. Nutr. Biochem. 2013, 24, 1415–1422. [Google Scholar] [CrossRef] [Green Version]
  55. Lee, H.C.; Jenner, A.M.; Low, C.S.; Lee, Y.K. Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota. Res. Microbiol. 2006, 157, 876–884. [Google Scholar] [CrossRef]
  56. Boto-Ordóñez, M.; Urpi-Sarda, M.; Queipo-Ortuño, M.I.; Tulipani, S.; Tinahones, F.J.; Andres-Lacueva, C. High levels of Bifidobacteria are associated with increased levels of anthocyanin microbial metabolites: A randomized clinical trial. Food Funct. 2014, 5, 1932–1938. [Google Scholar] [CrossRef] [PubMed]
  57. Hervert-Hernández, D.; Pintado, C.; Rotger, R.; Goñi, I. Stimulatory role of grape pomace polyphenols on Lactobacillus acidophilus growth. Int. J. Food Microbiol. 2009, 136, 119–122. [Google Scholar] [CrossRef] [PubMed]
  58. Saulnier, D.M.; Kolida, S.; Gibson, G.R. Microbiology of the human intestinal tract and approaches for its dietary modulation. Curr. Pharm. Des. 2009, 15, 1403–1414. [Google Scholar] [CrossRef] [PubMed]
  59. Rabot, S.; Membrez, M.; Bruneau, A.; Gerard, P.; Harach, T.; Moser, M.; Raymond, F.; Mansourian, R.; Chou, C.J. Germ-free C57BL/6J mice are resistant to high-fat-diet-induced insulin resistance and have altered cholesterol metabolism. FASEB J. 2010, 24, 4948–4959. [Google Scholar] [CrossRef] [Green Version]
  60. Tahri, K.; Crociani, J.; Ballongue, J.; Schneider, F. Effects of three strains of bifidobacteria on cholesterol. Lett. Appl. Microbiol. 1995, 21, 149–151. [Google Scholar] [CrossRef]
  61. Hidalgo, M.; Concha, M.J.O.; Kolida, S.; Walton, G.E.; Kallithraka, S.; Spencer, J.P.E.; Gibson, G.R.; De Pascual-Teresa, S. Metabolism of Anthocyanins by Human Gut Microflora and Their Influence on Gut Bacterial Growth. J. Agric. Food Chem. 2012, 60, 3882–3890. [Google Scholar] [CrossRef]
  62. Tzounis, X.; Rodriguez-Mateos, A.; Vulevic, J.; Gibson, G.R.; Kwik-Uribe, C.; Spencer, J.P. Prebiotic evaluation of cocoa-derived flavanols in healthy humans by using a randomized, controlled, double-blind, crossover intervention study. Am. J. Clin. Nutr. 2011, 93, 62–72. [Google Scholar] [CrossRef]
  63. Righelato, R. Beer: Quality, safety and nutritional aspects. PS Hughes and ED Baxter. Royal Society of Chemistry, Cambridge, 2001. 138 pp ISBN 0-85404-588-0. J. Sci. Food Agric. 2004, 84, 608. [Google Scholar] [CrossRef]
  64. Cheiran, K.P.; Raimundo, V.P.; Manfroi, V.; Anzanello, M.J.; Kahmann, A.; Rodrigues, E.; Frazzon, J. Simultaneous identification of low-molecular weight phenolic and nitrogen compounds in craft beers by HPLC-ESI-MS/MS. Food Chem. 2019, 286, 113–122. [Google Scholar] [CrossRef]
  65. Samuels, J.S.; Shashidharamurthy, R.; Rayalam, S. Novel anti-obesity effects of beer hops compound xanthohumol: Role of AMPK signaling pathway. Nutr. Metab. 2018, 15, 42. [Google Scholar] [CrossRef]
  66. Wei, S.; Sun, T.; Du, J.; Zhang, B.; Xiang, D.; Li, W. Xanthohumol, a prenylated flavonoid from Hops, exerts anticancer effects against gastric cancer in vitro. Oncol. Rep. 2018, 40, 3213–3222. [Google Scholar] [CrossRef] [PubMed]
  67. Kok, B.P.; Galmozzi, A.; Littlejohn, N.K.; Albert, V.; Godio, C.; Kim, W.; Kim, S.M.; Bland, J.S.; Grayson, N.; Fang, M.; et al. Intestinal bitter taste receptor activation alters hormone secretion and imparts metabolic benefits. Mol. Metab. 2018, 16, 76–87. [Google Scholar] [CrossRef] [PubMed]
  68. Liu, M.; Yin, H.; Liu, G.; Dong, J.; Qian, Z.; Miao, J. Xanthohumol, a Prenylated Chalcone from Beer Hops, Acts as an α-Glucosidase Inhibitor in Vitro. J. Agric. Food Chem. 2014, 62, 5548–5554. [Google Scholar] [CrossRef] [PubMed]
  69. Bartmańska, A.; Tronina, T.; Popłoński, J.; Milczarek, M.; Filip-Psurska, B.; Wietrzyk, J. Highly Cancer Selective Antiproliferative Activity of Natural Prenylated Flavonoids. Molecules 2018, 23, 2922. [Google Scholar] [CrossRef] [PubMed]
  70. Bartmanska, A.; Walecka-Zacharska, E.; Tronina, T.; Poplonski, J.; Sordon, S.; Brzezowska, E.; Bania, J.; Huszcza, E. Antimicrobial Properties of Spent Hops Extracts, Flavonoids Isolated Therefrom, and Their Derivatives. Molecules 2018, 23, 59. [Google Scholar] [CrossRef]
  71. Roehrer, S.; Stork, V.; Ludwig, C.; Minceva, M.; Behr, J. Analyzing bioactive effects of the minor hop compound xanthohumol C on human breast cancer cells using quantitative proteomics. PLoS ONE 2019, 14, e0213469. [Google Scholar] [CrossRef]
  72. Possemiers, S.; Heyerick, A.; Robbens, V.; De Keukeleire, D.; Verstraete, W. Activation of Proestrogens from Hops (Humulus lupulus L.) by Intestinal Microbiota; Conversion of Isoxanthohumol into 8-Prenylnaringenin. J. Agric. Food Chem. 2005, 53, 6281–6288. [Google Scholar] [CrossRef]
  73. Possemiers, S.; Bolca, S.; Grootaert, C.; Heyerick, A.; Decroos, K.; Dhooge, W.; De Keukeleire, D.; Rabot, S.; Verstraete, W.; Van De Wiele, T. The prenylflavonoid isoxanthohumol from hops (Humulus lupulus L.) is activated into the potent phytoestrogen 8-prenylnaringenin in vitro and in the human intestine. J. Nutr. 2006, 136, 1862–1867. [Google Scholar] [CrossRef]
  74. Possemiers, S.; Rabot, S.; Espín, J.C.; Bruneau, A.; Philippe, C.; González-Sarrías, A.; Heyerick, A.; Tomás-Barberán, F.A.; De Keukeleire, D.; Verstraete, W. Eubacterium limosum activates isoxanthohumol from hops (Humulus lupulus L.) into the potent phytoestrogen 8-prenylnaringenin in vitro and in rat intestine. J. Nutr. 2008, 138, 1310–1316. [Google Scholar]
  75. Paraiso, I.L.; Plagmann, L.S.; Yang, L.; Zielke, R.; Gombart, A.F.; Maier, C.S.; Sikora, A.E.; Blakemore, P.R.; Stevens, J.F. Back cover: Reductive Metabolism of Xanthohumol and 8-Prenylnaringenin by the Intestinal Bacterium Eubacterium ramulus. Mol. Nutr. Food Res. 2019, 63, e1800923. [Google Scholar] [CrossRef]
  76. Blatchford, P.A.; Parkar, S.G.; Hopkins, W.; Ingram, J.R.; Sutton, K.H. Dose-Dependent Alterations to In Vitro Human Microbiota Composition and Butyrate Inhibition by a Supercritical Carbon Dioxide Hops Extract. Biomolecules 2019, 9, 390. [Google Scholar] [CrossRef] [PubMed]
  77. Couteau, D.; McCartney, A.; Gibson, G.; Williamson, G.; Faulds, C. Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid. J. Appl. Microbiol. 2001, 90, 873–881. [Google Scholar] [CrossRef] [PubMed]
  78. Teixeira, C.; Prykhodko, O.; Alminger, M.; Hållenius, F.F.; Nyman, M. Barley Products of Different Fiber Composition Selectively Change Microbiota Composition in Rats. Mol. Nutr. Food Res. 2018, 62, e1701023. [Google Scholar] [CrossRef] [PubMed]
  79. Ou, J.-Y.; Huang, J.-Q.; Song, Y.; Yao, S.-W.; Peng, X.-C.; Wang, M.-F.; Ou, S.-Y. Feruloylated Oligosaccharides from Maize Bran Modulated the Gut Microbiota in Rats. Plant Foods Hum. Nutr. 2016, 71, 123–128. [Google Scholar] [CrossRef]
  80. Ma, Y.; Chen, K.; Lv, L.; Wu, S.; Guo, Z. Ferulic acid ameliorates nonalcoholic fatty liver disease and modulates the gut microbiota composition in high-fat diet fed ApoE−/− mice. Biomed. Pharmacother. 2019, 113, 108753. [Google Scholar] [CrossRef]
  81. Kutschera, M.; Engst, W.; Blaut, M.; Braune, A. Isolation of catechin-converting human intestinal bacteria. J. Appl. Microbiol. 2011, 111, 165–175. [Google Scholar] [CrossRef]
  82. Takagaki, A.; Nanjo, F. Bioconversion of (-)-epicatechin, (+)-epicatechin, (-)-catechin, and (+)-catechin by (-)-epigallocatechin-metabolizing bacteria. Biol. Pharm. Bull. 2015, 38, 789–794. [Google Scholar] [CrossRef]
  83. Krumholz, L.R.; Bryant, M.P. Eubacterium oxidoreducens sp. nov. requiring H2 or formate to degrade gallate, pyrogallol, phloroglucinol and quercetin. Arch. Microbiol. 1986, 144, 8–14. [Google Scholar] [CrossRef]
  84. Etxeberria, U.; Arias, N.; Boqué, N.; Macarulla, M.; Portillo, M.; Martinez, J.; Milagro, F. Reshaping faecal gut microbiota composition by the intake of trans-resveratrol and quercetin in high-fat sucrose diet-fed rats. J. Nutr. Biochem. 2015, 26, 651–660. [Google Scholar] [CrossRef]
  85. Cheng, K.-J.; Simpson, F.J.; Watkin, J.E.; Krishnamurty, H.G.; Jones, G.A. Identification of products produced by the anaerobic degradation of rutin and related flavonoids by Butyrivibrio sp. C3. Can. J. Microbiol. 1970, 16, 759–767. [Google Scholar]
  86. Quifer-Rada, P.; Martínez-Huélamo, M.; Chiva-Blanch, G.; Jáuregui, O.; Estruch, R.; Lamuela-Raventos, R.M. Urinary Isoxanthohumol Is a Specific and Accurate Biomarker of Beer Consumption. J. Nutr. 2014, 144, 484–488. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  87. Braune, A.; Engst, W.; Elsinghorst, P.W.; Furtmann, N.; Bajorath, J.; Gütschow, M.; Blaut, M. Chalcone Isomerase from Eubacterium ramulus Catalyzes the Ring Contraction of Flavanonols. J. Bacteriol. 2016, 198, 2965–2974. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  88. Tomás-Barberán, F.A.; Selma, M.V.; Espín, J.C. Interactions of gut microbiota with dietary polyphenols and consequences to human health. Curr. Opin. Clin. Nutr. Metab. Care 2016, 19, 471–476. [Google Scholar] [CrossRef] [PubMed]
  89. Tomás-Barberán, F.A.; Espín, J.C. Effect of Food Structure and Processing on (Poly)phenol–Gut Microbiota Interactions and the Effects on Human Health. Annu. Rev. Food Sci. Technol. 2019, 10, 221–238. [Google Scholar] [CrossRef] [PubMed]
  90. Selma, M.V.; Espín, J.C.; Tomás-Barberán, F.A. Interaction between Phenolics and Gut Microbiota: Role in Human Health. J. Agric. Food Chem. 2009, 57, 6485–6501. [Google Scholar] [CrossRef] [PubMed]
  91. Hernandez-Quiroz, F.; Nirmalkar, K.; Villalobos-Flores, L.E.; Murugesan, S.; Cruz-Narvaez, Y.; Rico-Arzate, E.; Hoyo-Vadillo, C.; Chavez-Carbajal, A.; Pizano-Zarate, M.L.; Garcia-Mena, J. Influence of moderate beer consumption on human gut microbiota and its impact on fasting glucose and beta-cell function. Alcohol 2019. [Google Scholar] [CrossRef]
  92. Miranda, C.L.; Johnson, L.A.; De Montgolfier, O.; Elias, V.D.; Ullrich, L.S.; Hay, J.J.; Paraiso, I.L.; Choi, J.; Reed, R.L.; Revel, J.S.; et al. Non-estrogenic Xanthohumol Derivatives Mitigate Insulin Resistance and Cognitive Impairment in High-Fat Diet-induced Obese Mice. Sci. Rep. 2018, 8, 613. [Google Scholar] [CrossRef]
  93. Delmulle, L.; Bellahcene, A.; Dhooge, W.; Comhaire, F.; Roelens, F.; Huvaere, K.; Heyerick, A.; Castronovo, V.; De Keukeleire, D. Anti-proliferative properties of prenylated flavonoids from hops (Humulus lupulus L.) in human prostate cancer cell lines. Phytomedicine 2006, 13, 732–734. [Google Scholar] [CrossRef]
  94. Mohib, M.M.; Rabby, S.F.; Paran, T.Z.; Hasan, M.M.; Ahmed, I.; Hasan, N.; Sagor, M.A.T.; Mohiuddin, S. Protective role of green tea on diabetic nephropathy—A review. Cogent Boil. 2016, 2, 1248166. [Google Scholar]
Table
Table 1. Classification of the phenolic compounds in beer.
Table 1. Classification of the phenolic compounds in beer.
ClassGroupCongenersConcentration
MonophenolsPhenolic alcoholsTyrosol3–40 mg/L
Phenolic acidsp-coumaric acid, ferulic, vanillic, gallic, caffeic, syringic, sinapic acids, etc.10–30 mg/L (including esters and glycosides)
Phenolic amines and amino acidsHordenine, tyramine, N-methyltyramine, tyrosamine, tyrosine10–20 mg/L (3–8 mg/L as tyrosine)
Monomeric polyphenolsFlavonoids catechines (flavan-3-ol)(+) catechin (+) epicatechin (possibly other isomers)0.5–13 mg/L 1–10 mg/L
Prenylated flavonoidXanthohumol, isoxanthohumol, etc.0.002–3.5 mg/L
α-acids and iso-α-acidsCohumulone, n-humulone, adhumulone, and iso-cohumulone, iso-n-humulone, iso-adhumulone2.3–100 mg/L
AnthocyanogensLeucocyanidin4–80 mg/L
FlavonolsQuercetin, kaempferol, myricetin (occur as glycosides), rutin, etc.less than 10 mg/L
Condensed polyphenols Dimeric catechins5–8 mg/L
Trimersless than 1 mg/L
Adapted data from [45] and [18].
Table
Table 2. Relationship between polyphenols within beer and gut microbiota.
Table 2. Relationship between polyphenols within beer and gut microbiota.
ClassPhenolic CompoundMetaboliteResponsible Intestinal Microbiota and Implication Experimental Conditions, Methodology and Tested OrganismReference
Prenylated flavonoidsIsoxanthohumol8-prenylnaringeninEubacterium limosumIn vitro (fecal human culture + isolate). HPLC Analysis
In vivo (human and mouse) SHIME run, real-time PCR, HPLC analysis		Possemiers et al. 2005 [72]
Possemiers et al. 2006 [73]
Possemiers et al. 2008 [74]
Xanthohumolα,β-dihydroxanthohumol Eubacterium ramulusIn vitro (bacterial culture of E. limosum and E. ramulus)Paraiso et al. 2019 [75]
Bitter acidsα- and β-acidsCohumulone, adhumulone, colupulone, lupulone, adlupuloneIncrease of Enterobacteriaceae and Akkermansia
Decrease of butyrate producers: Eubacterium and Coprococcus as well as Bifidobacterium		Hops extract fermentation in pH-controlled anaerobic batch fermenter with a human fecal inoculum
16SrRNA sequencing and qPCR		Blatchford et al. 2019 [76]
Monophenol. Phenolic acidsFerulic acid Escherichia coli Bifidobacterium lactis
Lactobacillus gasseri
Increase the α-diversity
In vitro (fecal human culture + isolate). 16SrRNA sequencing
In vivo (mouse, dietary fiber from barley malts)
In vivo (mouse). 16S rRNA sequencing		Couteau et al. 2001 [77]
Teixeira et al. 2018 [78]
Ou et al. 2016 [79]
indole-3-acetic acidModulates ratio Firmicutes/BacteroidetesIn vivo (mouse). 16S rRNA sequencing Ma et al. 2019 [80]
Monomeric polyphenols. Flavan-3-olCatechin and Epicatechin1-(3,4-dihydroxy-phenyl)-3-(2,4,6-trihydroxyphenyl)propan-2-ol
5-(3,4-Dihydroxyphenyl)-g-valerolactone
4-Hydroxy-5-(3,4-dihydroxy-phenyl)valeric acid		Eggerthella lenta
Flavonifractor plautiia		In vitro (fecal human culture + isolate). 16SrRNA sequencing
In vitro (fecal rats culture + isolate)
16SrRNA sequencing
LC/MS and LC/MS/MS analyses		Kutschera et al. 2011 [81]
Takagaki et al. 2015 [82]
Condensed polyphenols. Dimeric catechinsProanthocyanidins1-(3, 4, 5-trihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol
1-(3, 5-dihy-droxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol 		Adlercreutzia equolifaciens
Eggerthella lenta		In vitro (fecal rats culture + isolate)
16SrRNA sequencing
LC/MS and LC/MS/MS analyses		Takagaki et al. 2015 [82]
Monomeric polyphenols. FlavonolsQuercetin3,4-dihydroxyphenylacetateEubacterium oxidoreducens
Decrease:
Firmicutes/Bacteroidetes
Decrease:
Erysipelotrichaceae
Bacillus
Eubacterium cylindroides		In vitro (Bacterial culture). Thin layer chromatography (TLC)
In vivo (mouse). polymerase chain reaction (PCR) and bacterial 16SrDNA pyrosequencing		Krumholz & Bryant 1986 [83]
Etxeberria et al. 2015 [84]
Rutin 3,4-dihydroxy-benzaldehyde
3,4-dihydroxyphenylacetic acid		ButyrivibrioIn vitro (Bacterial culture) Chromatography
(Sheep)		Krishnamurty et al. 1970 [85]

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MDPI and ACS Style

Quesada-Molina, M.; Muñoz-Garach, A.; Tinahones, F.J.; Moreno-Indias, I. A New Perspective on the Health Benefits of Moderate Beer Consumption: Involvement of the Gut Microbiota. Metabolites 2019, 9, 272. https://doi.org/10.3390/metabo9110272

AMA Style

Quesada-Molina M, Muñoz-Garach A, Tinahones FJ, Moreno-Indias I. A New Perspective on the Health Benefits of Moderate Beer Consumption: Involvement of the Gut Microbiota. Metabolites. 2019; 9(11):272. https://doi.org/10.3390/metabo9110272

Chicago/Turabian Style

Quesada-Molina, Mar, Araceli Muñoz-Garach, Francisco J. Tinahones, and Isabel Moreno-Indias. 2019. "A New Perspective on the Health Benefits of Moderate Beer Consumption: Involvement of the Gut Microbiota" Metabolites 9, no. 11: 272. https://doi.org/10.3390/metabo9110272

APA Style

Quesada-Molina, M., Muñoz-Garach, A., Tinahones, F. J., & Moreno-Indias, I. (2019). A New Perspective on the Health Benefits of Moderate Beer Consumption: Involvement of the Gut Microbiota. Metabolites, 9(11), 272. https://doi.org/10.3390/metabo9110272

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