Enhancing the Productivity and Stability of Superoxide Dismutase from Saccharomyces cerevisiae TBRC657 and Its Application as a Free Radical Scavenger
, Thidarat Nimchua 2 and Prakit Sukyai 1,*Round 1
Reviewer 1 Report
The authors show data on the different steps for production, isolation and stabilization of SOD from a specific strain of S. cerevisiae. The manuscript is well written, although it could be strongly reduced without impacting on the reader comprehension of the main message.
major concern:
- the data on the protective effect of SOD on cell viability of fibroblast, or its putative toxic effect, lacks statistical analysis for a real understanding of the differences observed. Moreover, the data on the protective effect of SOD on the oxidative induced stress by hydrogen peroxide on fibroblasts is over interpreted. I am not convinced that the authors have data to say that “SOD had the potential to eliminate the ROS produced inside the cell…. “(pag 475 line 464-466). In my opinion this data should be left out of the manuscript as its interpretation is questionable, and it does not add any other layer of complexity in the data presented.
- in the tables it is missing the meaning of the small letters (a, b, ….) probably are related with statistical analysis.
Author Response
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Author Response.pdf
Reviewer 2 Report
Comments for author File: Comments.pdf
Author Response
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Author Response File: Author Response.pdf
