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Agronomy
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Emerging Topics in Tobacco Genomics
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Emerging Topics in Tobacco Genomics

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 8537

Special Issue Editor

Prof. Dr. Yongfeng Guo

E-Mail Website
Guest Editor
Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Beijing 266101, China
Interests: Plant senescence, Peptide signaling, Tobacco biotechnology, Systems biology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

As important model plants and economic crops, tobacco (Nicotiana tabacum and related Nicotiana species) species have drawn a significant amount of attention in the field of plant biology. In recent years, several versions of tobacco genome sequences have become available and significant progress has been made in tobacco functional genomics.

This Special Issue aims to gather new information about the systems biology of tobacco, genes and molecular regulatory networks that regulate important traits, and new molecular tools developed based on tobacco, as well as cutting-edge research using tobacco as model systems.

Specifically, this Special Issue calls for original research, reviews, and mini review submissions on tobacco functional genomics, including but not limited to: tobacco omics and gene families; cloning and functional analysis of genes; analysis of tobacco mutants; characterization of plant genes using tobacco as model systems; synthetic biology involving tobacco; new tools in molecular biology and biotechnology based on tobacco.

Prof. Dr. Yongfeng Guo
Guest Editor

Manuscript Submission Information

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Keywords

  • tobacco
  • genome
  • transcriptome
  • gene function
  • stress response
  • bioreactor
 

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Published Papers (5 papers)

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Research

19 pages, 46671 KiB  
Article
Integrative Analysis of the DUF668 Gene Family in Nicotiana tabacum to Excavate Their Potential Roles in Abiotic Stress Responses
by Zhenbiao Zhang, Zhongqi Zhang, Sayed Abdul Akher, Jin Xue, Jie Wang, Cun Guo, Zhiyuan Li and Yongfeng Guo
Agronomy 2024, 14(3), 445; https://doi.org/10.3390/agronomy14030445 - 24 Feb 2024
Viewed by 1358
Abstract
The domain of the unknown function (DUF) gene families assumes pivotal roles in plant metabolic and stress responses. However, our comprehension of the tobacco DUF668 (NtDUF668) gene family and its specific reactions to heavy metal, drought, and salt stresses [...] Read more.
The domain of the unknown function (DUF) gene families assumes pivotal roles in plant metabolic and stress responses. However, our comprehension of the tobacco DUF668 (NtDUF668) gene family and its specific reactions to heavy metal, drought, and salt stresses remain circumscribed. In the current investigation, a comprehensive genome-wide analysis of the NtDUF668 gene family was undertaken utilizing bioinformatics tools. The results unveiled a total of 20 members in the NtDUF668 gene family, denominated NtDUF668-01 to NtDUF668-20. Phylogenetic analyses indicated a closer genetic relationship of DUF668 genes between Nicotiana tabacum and Ipomoea batatas. The examination of gene structure and conservative motifs revealed a bifurcation into two major Clades, aligning with previous studies on DUF668 gene families from various plant species, emphasizing its highly conserved evolutionary mechanism across plants. The exploration of promoter regions of NtDUF668 genes revealed a plethora of cis-acting elements associated with abiotic and biotic stresses, light signaling, and phytohormones. Gene duplication events and selection pressure analysis disclosed the segmental duplication and strong purifying selection pressure during the evolution of NtDUF668 genes. Syntenic analysis indicated a relatively conserved evolutionary mechanism of DUF668 gene families within dicotyledons. Tissue-specific expression analysis suggested that NtDUF668 family members are potentially involved in root development, floral organ formation, and abscission. The expression patterns and qRT–PCR analysis of NtDUF668 genes implied the potentially functional involvements of NtDUF668s in response to multiple abiotic stresses. Furthermore, the stress-triggered member NtDUF668-08 exhibited specific nuclear localization. In conclusion, this genome-wide analysis illuminates the composition, phylogenetic relationships, and potential roles of the NtDUF668 gene family in abiotic stress responses. The identified candidate genes, particularly NtDUF668-08, warrant further research for functional investigation. Full article
(This article belongs to the Special Issue Emerging Topics in Tobacco Genomics)
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17 pages, 6187 KiB  
Article
Phenotypic Characterization and Gene Mapping of a Spiral Leaf and Dwarf (sld) Mutant from Tetraploid Common Tobacco (Nicotiana tabacum L.)
by Shaomei Wang, Xinru Wu, Yongfeng Guo, Dawei Wang, Lirui Cheng, Yuanying Wang, Aiguo Yang and Guanshan Liu
Agronomy 2023, 13(9), 2354; https://doi.org/10.3390/agronomy13092354 - 11 Sep 2023
Viewed by 1506
Abstract
Leaf morphology and plant height are two agronomic traits closely related to tobacco (Nicotiana tabacum L.) yield and quality. The study of leaf morphology and plant stature mutants will greatly contribute to the fields of plant architecture breeding and developmental biology. Here, [...] Read more.
Leaf morphology and plant height are two agronomic traits closely related to tobacco (Nicotiana tabacum L.) yield and quality. The study of leaf morphology and plant stature mutants will greatly contribute to the fields of plant architecture breeding and developmental biology. Here, we report the characterization of a spiral leaf and dwarf (sld) mutant identified from an ethylmethane sulfonate (EMS)-induced common tobacco population. The sld mutant displayed the phenotype of wrinkled, spiral, and miniature leaves, with the growth point as the central axis and plant dwarfing with shortened internodes. The inheritance pattern of the sld mutant phenotype was manipulated by a recessive nuclear monogene, which was linked to six tobacco simple sequence repeat (SSR) markers from linkage group 5 via gene mapping. Utilizing an F2 population, the sld mutant gene the sld mutant gene was located between the co-segregated markers PT51778, PT54913, and the marker PT61414, with an equal genetic distance of 0.16 cM. Taking advantage of a BC1F1 population, the markers PT51778, PT54913, the sld gene, and the marker PT61414 demonstrated co-segregation, located between the markers PT40040 and PT60933, respectively, with a genetic distance of 1.37 cM and 6.32 cM, respectively. These findings will be helpful in cloning the sld gene and in the further characterization of the regulatory genes controlling the spiral and dwarfing phenotypes in tobacco. Full article
(This article belongs to the Special Issue Emerging Topics in Tobacco Genomics)
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16 pages, 3865 KiB  
Article
A Cationic Amino Acid Transporter NtCAT1 Promotes Leaf Senescence by the Accumulation of ABA in Nicotiana tabacum
by Songchong Lu, Xuan Ji, Xinshuang Zhang, Hao Wu, Yan Sun, Ying Zhu, Shanshan Su, Shumin Wei and Xin Liu
Agronomy 2023, 13(7), 1691; https://doi.org/10.3390/agronomy13071691 - 24 Jun 2023
Cited by 3 | Viewed by 1370
Abstract
Leaf senescence is a comprehensive process performed by integrating various internal and external signals, followed by nutrient reallocation, especially via organic nitrogen (e.g., amino acids) mobilization. Amino acid (AA) transporters play an important role in crop growth and development by participating in the [...] Read more.
Leaf senescence is a comprehensive process performed by integrating various internal and external signals, followed by nutrient reallocation, especially via organic nitrogen (e.g., amino acids) mobilization. Amino acid (AA) transporters play an important role in crop growth and development by participating in the process of organic nitrogen remobilization. However, the biological functions and downstream effectors of amino acid transporters involved in leaf senescence are still poorly understood. In the present study, we cloned and characterized a cationic amino acid transporter gene, NtCAT1, from tobacco (K326). We found that NtCAT1 transcript levels were induced by age and abscisic acid (ABA). The NtCAT1 protein was highly localized in the plasma membrane. The overexpressing NtCAT1 line (OECAT1) showed early leaf senescence, accompanied with increased reactive oxygen species (ROS) and ABA content. By contrast, the NtCAT1 mutant (ntcat1-36) generated by the CRSPR/Cas9 system, showed a delayed-senescence phenotype with a decreased accumulation of ROS and ABA. Moreover, we discovered that the overexpression of NtCAT1 could downregulate the expression of the target of rapamycin (TOR) kinase gene and upregulate the transcript levels of ABA-related genes during leaf senescence compared with wild type (WT), while the expression of these genes in ntcat1-36 plants exhibited inverse trends. Furthermore, an analysis of the amino acid concentration demonstrated that NtCAT1 transgenic plants displayed dramatic changes in the amino acid profile during leaf senescence. In summary, our results suggest that NtCAT1 could promote leaf senescence via the increased biosynthesis of ABA, and our study provides new insights into the molecular mechanism of leaf senescence. Full article
(This article belongs to the Special Issue Emerging Topics in Tobacco Genomics)
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17 pages, 12253 KiB  
Article
Characterization of the CLE Family in Three Nicotiana Species and Potential Roles of CLE Peptides in Osmotic and Salt Stress Responses
by Yumeng Chu, Xiaoming Gao, Lichao Wen, Zhichao Deng, Tao Liu and Yongfeng Guo
Agronomy 2023, 13(6), 1480; https://doi.org/10.3390/agronomy13061480 - 27 May 2023
Viewed by 1976
Abstract
The CLE family (CLAVATA3/embryo surrounding region-related), a class of small secreted proteins, play important roles in plant development and stress responses. Members of the CLE family have been characterized in a number of plant species, including Arabidopsis and rice. However, limited information is [...] Read more.
The CLE family (CLAVATA3/embryo surrounding region-related), a class of small secreted proteins, play important roles in plant development and stress responses. Members of the CLE family have been characterized in a number of plant species, including Arabidopsis and rice. However, limited information is available about CLE peptides in tobacco (Nicotiana tabacum) and related Nicotiana species. Here we report the identification of 84 CLE family members in three Nicotiana species based on sequence similarity. The newly identified CLE members, including 41 from N. tabacum, 19 from N. sylvestris, and 24 from N. tomentosiformis, together with 32 CLEs from Arabidopsis and 52 CLEs from tomato, formed 9 subgroups in a phylogenic tree. The unbalanced distribution of the Nicotiana CLEs in the subgroups suggested potential preferential gene family expansion during evolution. Expression of the NtCLE genes was analyzed and a number of the NtCLEs showed induced expression upon abiotic stress treatments. Synthetic peptides of several NtCLEs, when applied to detached tobacco leaf discs, were able to increase plants’ tolerance to osmotic and salinity stresses, suggesting potential roles of CLE peptides in the stress responses of tobacco. Full article
(This article belongs to the Special Issue Emerging Topics in Tobacco Genomics)
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12 pages, 3117 KiB  
Article
Manipulation of CBTS1 Expression Alters Tobacco Resistance to Spodoptera frugiperda and Phytophthora nicotianae
by Jian Guan, Zaifeng Du, Tian Tian, Wenjing Wang, Fuzhu Ju, Xiaoyang Lin, Zhongfeng Zhang, Yi Cao and Hongbo Zhang
Agronomy 2023, 13(3), 845; https://doi.org/10.3390/agronomy13030845 - 14 Mar 2023
Cited by 2 | Viewed by 1488
Abstract
Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing CBTS1 [...] Read more.
Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing CBTS1 expression on tobacco resistance to the insect pest Spodoptera frugiperda and oomycete pathogen Phytophthora nicotianae. The CDS sequence of CBTS1 was cloned into gene overexpression and silencing vectors and introduced into tobacco (Nicotiana tabacum L. cv. TN90) to obtain CBTS1-overexpression plants (CBTS1-OE) and CBTS1-silenced plants (CBTS1-RI). Compared with control plants, the content of cembratrien-ol (CBT-ol) was increased 4.48 times in the CBTS1-OE plants but decreased by 68% in the CBTS1-RI plants, while that of cembratrien-diol (CBT-diol) was increased 3.17 times in the CBTS1-OE plants but decreased by 76% in the CBTS1-RI plants. The S. frugiperda resistance of transgenic tobacco plants was evaluated by in vitro toxicity test, and the results showed that the resistance of CBTS1-OE plants to S. frugiperda was significantly improved but that of CBTS1-RI plants was reduced. The P. nicotianae resistance of transgenic tobacco plants was assessed by the detached leaf assay, and the results showed that the resistance of CBTS1-OE plants to P. nicotianae was enhanced, while that of CBTS1-RI plants was attenuated. Further gene expression analysis showed that overexpression of CBTS1 increased the expression of the pathogen-related gene PR-1a, while silencing of CBTS1 decreased its expression. This study demonstrated that manipulating the expression of CBTS1 could change the cembranoid content in tobacco plants and alter their resistance to both insect pests and oomycete pathogens. Full article
(This article belongs to the Special Issue Emerging Topics in Tobacco Genomics)
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