Strategies for Combatting Multidrug-Resistant and Extensively Drug-Resistant Bacteria, 2nd Edition

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "The Global Need for Effective Antibiotics".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 2048

Special Issue Editors


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Guest Editor
Department of Microbiology, University of Wroclaw, Wroclaw, Poland
Interests: cross-resistance; antibiotic resistance; efflux pumps; Salmonella
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Guest Editor
Department of Microbiology, University of Wroclaw, Wroclaw, Poland
Interests: silver nanoparticles; alternative therapy; bacterial resistance

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Guest Editor
Institute of Microbiology, Faculty of Medicine, University Hospital Bratislava, Comenius University, 81108 Bratislava, Slovakia
Interests: drug-resistant bacteria; antimicrobial resistance; new therapeutic solutions; improvement of existing therapies and alternative therapies, such as autovaccines or bacteriophage therapy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The first volume of the Special Issue “Strategies for Combatting Multidrug-Resistant and Extensively Drug-Resistant Bacteria: Current Challenges and Future Prospects ” was published in October 2023. It is a successful issue with nine published papers, encouraging us to open a second volume on the same topic.

As a continuation of the first Special Issue, this second volume will also focus on new strategies, including those that are not only related to novel antimicrobial drugs, but also those related to uncommon drug strategies. The topics of interest include, but are not limited to, the following:

  • MDR bacteria;
  • EDR bacteria;
  • Novel antimicrobial drugs;
  • Novel therapeutical approaches against MDR;
  • Novel therapeutical approaches against EDR;
  • Guidelines;
  • Personalized microbiota;
  • Bacteriophages;
  • Autovaccine therapy;
  • Antimicrobial stewardship.

Dr. Bożena Futoma-Kołoch
Dr. Anna Kędziora
Dr. Adriána Liptáková
Guest Editors

Manuscript Submission Information

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Keywords

  • MDR bacteria
  • EDR bacteria
  • novel antimicrobial drugs
  • novel therapeutical approaches against MDR
  • novel therapeutical approaches against EDR
  • guidelines
  • personalized microbiota
  • bacteriophages
  • autovaccine therapy
  • antimicrobial stewardship

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Published Papers (2 papers)

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Research

18 pages, 5689 KiB  
Article
Four New Sequence Types and Molecular Characteristics of Multidrug-Resistant Escherichia coli Strains from Foods in Thailand
by Nalumon Thadtapong, Soraya Chaturongakul, Sithichoke Tangphatsornruang, Chutima Sonthirod, Natharin Ngamwongsatit and Ratchaneewan Aunpad
Antibiotics 2024, 13(10), 935; https://doi.org/10.3390/antibiotics13100935 - 2 Oct 2024
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Abstract
The presence of antibiotic-resistant Escherichia coli in food is a serious and persistent problem worldwide. In this study, 68 E. coli strains isolated from Thai food samples were characterized. Based on antibiotic susceptibility assays, 31 of these isolates (45.59%) showed multiple antibiotic resistance [...] Read more.
The presence of antibiotic-resistant Escherichia coli in food is a serious and persistent problem worldwide. In this study, 68 E. coli strains isolated from Thai food samples were characterized. Based on antibiotic susceptibility assays, 31 of these isolates (45.59%) showed multiple antibiotic resistance (MAR) index values > 0.2, indicating high exposure to antibiotics. Among these, strain CM24E showed the highest resistance (it was resistant to ten antibiotics, including colistin and imipenem). Based on genome sequencing, we identified four isolates (namely, CF25E, EF37E, NM10E1, and SF50E) with novel Achtman-scheme multi-locus sequence types (STs) (ST14859, ST14866, ST14753, and ST14869, respectively). Clermont phylogrouping was used to subtype the 68 researched isolates into five Clermont types, mainly A (51.47%) and B1 (41.18%). The blaEC gene was found only in Clermont type A, while the blaEC-13 gene was predominant in Clermont type B1. A correlation between genotypes and phenotypes was found only in Clermont type B1, which showed a strong positive correlation between the presence of an afa operon and yersiniabactin-producing gene clusters with the colistin resistance phenotype. Strain SM47E1, of Clermont type B2, carried the highest number of predicted virulence genes. In summary, this study demonstrates the pressing problems posed by the prevalence and potential transmission of antimicrobial resistance and virulence genes in the food matrix. Full article
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19 pages, 2073 KiB  
Article
Exploring Biofilm-Related Traits and Bile Salt Efficacy as Anti-Biofilm Agents in MDR Acinetobacter baumannii
by Verica Aleksic Sabo, Dušan Škorić, Suzana Jovanović-Šanta and Petar Knezevic
Antibiotics 2024, 13(9), 880; https://doi.org/10.3390/antibiotics13090880 - 13 Sep 2024
Viewed by 777
Abstract
Acinetobacter baumannii has been designated as a critical priority pathogen by the World Health Organization for the development of novel antimicrobial agents. This study aimed to investigate both the phenotypic and genotypic traits of multidrug-resistant (MDR) A. baumannii strains, along with the effects [...] Read more.
Acinetobacter baumannii has been designated as a critical priority pathogen by the World Health Organization for the development of novel antimicrobial agents. This study aimed to investigate both the phenotypic and genotypic traits of multidrug-resistant (MDR) A. baumannii strains, along with the effects of natural bile salts on biofilm formation. The research analyzed phenotypic traits, including autoaggregation, hydrophobicity, twitching motility, lectin production, and biofilm formation, as well as genotypic traits such as the presence of bap and blaPER-1 genes in twenty wound and eight environmental MDR A. baumannii isolates. While all strains were identified as good biofilm producers, no statistically significant correlation was detected between the examined traits and biofilm formation. However, differences in biofilm production were observed between environmental and wound isolates. The natural bile salts Na-cholate, Na-deoxycholate, and Na-chenodeoxycholate demonstrated effective anti-A. baumannii activity (MIC = 0.25–10 mg mL−1), with significant anti-biofilm effects. Na-deoxycholate and Na-chenodeoxycholate inhibited 94–100% of biofilm formation at super-MIC concentrations (8–32 mg mL−1). This study underscores the urgent need for innovative strategies to combat antibiotic resistance and biofilm formation in A. baumannii, highlighting the potential of natural bile salts as promising biofilm inhibitors and encouraging further research into their modification and combination with other antimicrobials. Full article
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