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Phytochemical Compounds and Antioxidant Activity

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Food Science and Technology".

Deadline for manuscript submissions: 20 June 2025 | Viewed by 1399

Special Issue Editors


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Guest Editor
Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Interests: medicinal plants; phytochemistry; natural product chemistry; chromatography; secondary metabolites; bioactivity

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Guest Editor
Department of Analytical Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Interests: elemental analysis; instrumental techniques (TXRF, EDXRF, ICP-MS, ICP-OES, AAS); sample preparation; green analytical chemistry

Special Issue Information

Dear Colleagues,

We are currently facing an surge in the usage of plant-derived products as a source of phytochemical compounds for various medicinal problems. The fact that approximately 80% of the world´s population use some form of traditional medicine, including phytotherapy, attests to the need for constant research in this area. A sedentary lifestyle, stress, a lack of free time, and an unbalanced diet lead to the generation of oxidative stress in our body. The overproduction of oxidants may cause an imbalance that results in diseases such as dyslipidemia, high blood pressure, obesity, diabetes, cancer, and neurodegenerative diseases. Plants are a rich source of ingredients, including various biologically active organic components such as phenolic acids, flavonoids, tannins, vitamins and minerals (macro-, micro- and trace elements). The content of each of these components in plants can contribute to their antioxidant capacity.

This Special Issue is dedicated to the extraction, chemical characterization, and quantification of phytochemical compounds, and their potential in maintaining redox balance.

In this Special Issue, we welcome original research papers and reviews that are focused on a range of plant extraction processes, especially green extraction methods, phytochemical analysis using various analytical techniques, the determination of the composition of trace elements that may be beneficial due to their antioxidant potential, and in vitro and in vivo assays to clarify the molecular mechanisms of phytochemicals.

We look forward to your contribution.

Sincerely,

Dr. Maja Bival Štefan
Dr. Jasna Jablan
Guest Editors

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Keywords

  • phytochemicals
  • antioxidant
  • polyphenols
  • minerals
  • metabolic diseases
  • chromatographic analysis of antioxidants
  • spectroscopic analysis of antioxidants
  • analysis of mineral content

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Published Papers (1 paper)

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Research

13 pages, 1858 KiB  
Article
Enhancement of Phenylpropanoid Accumulation and Antioxidant Activities of Agastache rugosa Transgenic Hairy Root Cultures by Overexpressing the Maize Lc Transcription Factor
by Bao Van Nguyen, Jae Kwang Kim, Jinsu Lim, Kihyun Kim, Ramaraj Sathasivam, Dong Ha Cho and Sang Un Park
Appl. Sci. 2024, 14(20), 9617; https://doi.org/10.3390/app14209617 - 21 Oct 2024
Viewed by 1117
Abstract
Agastache rugosa is also known as Korean mint, and it has numerous health benefits due to its rich source of phenolic compounds. The main objective of this study was to produce a ZmLC-overexpressing transgenic hairy root line via Agrobacterium rhizogenes-mediated transformation. [...] Read more.
Agastache rugosa is also known as Korean mint, and it has numerous health benefits due to its rich source of phenolic compounds. The main objective of this study was to produce a ZmLC-overexpressing transgenic hairy root line via Agrobacterium rhizogenes-mediated transformation. The overexpressing transgenic lines were screened using qRT-PCR after exposure to light conditions. The best hairy root line was selected, and the expression levels of phenylpropanoid biosynthetic pathway genes and phenylpropanoid compound accumulation were analysed using qRT-PCR and HPLC, respectively. In addition, antioxidant activities (RPA, ABTS, and DPPH), total phenolic content, and total flavonoid content were analysed. The ZmLC-overexpressing transgenic line upregulated all the phenylpropanoid pathway genes, which led to the higher accumulation of phenylpropanoid compounds in the transgenic line than in the control line. In addition, the total phenolic and flavonoid content was significantly higher in the transgenic line. The antioxidant activity assay showed that the transgenic hairy root line had significantly higher activity than that of the control lines. Thus, ZmLC positively enhances the phenylpropanoid biosynthetic pathway and antioxidant activities in A. rugosa. The results show that ZmLC can be used to enhance phenylpropanoid compounds and antioxidant activities in transgenic A. rugosa hairy root lines via the genetic engineering approach. Full article
(This article belongs to the Special Issue Phytochemical Compounds and Antioxidant Activity)
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