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Biology
Special Issues
Transgenic and Genome Editing in Fish
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Transgenic and Genome Editing in Fish

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Biotechnology".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 7860

Special Issue Editors

Prof. Dr. Mingyou Li

E-Mail Website
Guest Editor
Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China
Interests: fish germ cells; fish stem cells; cell transplantation; fish sex determination and differentiation
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Huapu Chen

E-Mail Website
Guest Editor
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
Interests: fish reproduction; fish sex determination and differentiation; fish genetic analysis
Prof. Dr. Jianzhen Li

E-Mail Website
Guest Editor
College of Life Sciences, Northwest Normal University, Lanzhou 730070, China
Interests: hormone; fish germ cells; fish reproduction; fish gonadal development
Dr. Minghui Li

E-Mail Website
Guest Editor
College of Life Sciences, Southwest University, Chongqing 400715, China
Interests: fish sex determination and differentiation; sexual plasticity; fish reproductive endocrinology

Special Issue Information

Dear Colleagues,

Among all vertebrates, fish are the most diverse group, with over 32,000 species recorded - half of all vertebrates on Earth. Because fish provide us with a large amount of protein, it is important to understand their biology. In the last decade, many important breakthroughs have been made. Examples, the transgenic and genome editing technologies. Using the trangenic method, controllable primordial germ cells' on-off strategies for producing sterile offspring and fertile parents were obtained, which is of great use in aquaculture. While genome editing technology is a useful tool for functional genomics research. Gene knockouts help to identify candidate genes important for the development of specific economic traits such as growth disease resistance and intermuscular bone development. Understanding gene function can also help in selecting the best parents for selective breeding programs and commercial production. In this Special Issue, we will collect desired progress in transgenic and gene editing in fish.

We look forward to receiving your contributions.

Prof. Dr. Mingyou Li
Prof. Dr. Huapu Chen
Prof. Dr. Jianzhen Li
Dr. Minghui Li
Guest Editors

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

 

Keywords

  • transgenic
  • genome/gene editing
  • gene knock out/in
  • functional genomics
  • gene correction or modification

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Published Papers (3 papers)

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Research

17 pages, 8725 KiB  
Article
Effects of Myostatin b Knockout on Offspring Body Length and Skeleton in Yellow Catfish (Pelteobagrus fulvidraco)
by Xincheng Zhang, Fang Wang, Mi Ou, Haiyang Liu, Qing Luo, Shuzhan Fei, Jian Zhao, Kunci Chen, Qingshun Zhao and Kaibin Li
Biology 2023, 12(10), 1331; https://doi.org/10.3390/biology12101331 - 12 Oct 2023
Cited by 3 | Viewed by 1949
Abstract
Based on obtaining mstnb gene knockout in Pelteobagrus fulvidraco, a study on the effect of the mstn gene on skeletal morphology and growth was performed by comparing the number and length of the vertebrae of mutant and wild-type fish in a sibling [...] Read more.
Based on obtaining mstnb gene knockout in Pelteobagrus fulvidraco, a study on the effect of the mstn gene on skeletal morphology and growth was performed by comparing the number and length of the vertebrae of mutant and wild-type fish in a sibling group of P. fulvidraco, combined with the differences in cells at the level of vertebral skeletal tissue. It was found that mstnb gene knockdown resulted in a reduction in the number of vertebrae, the length, and the intervertebral distance in P. fulvidraco, and these changes may be the underlying cause of the shorter body length in mutant P. fulvidraco. Further, histological comparison of the same sites in the mstn mutant and wild groups of P. fulvidraco also revealed that the number and density of osteocytes were greater in mstnb knockout P. fulvidraco than in wild-type P. fulvidraco. Our results demonstrated that when using genome editing technology to breed new lines, the effects of knockout need to be analyzed comprehensively and may have some unexpected effects due to insufficient study of the function of certain genes. Full article
(This article belongs to the Special Issue Transgenic and Genome Editing in Fish)
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13 pages, 8594 KiB  
Article
hoxa1a-Null Zebrafish as a Model for Studying HOXA1-Associated Heart Malformation in Bosley–Salih–Alorainy Syndrome
by Hongjie Wang, Jingwei He, Xuemei Han, Xiuzhi Wu, Xuebin Ye, Wenchao Lv and Yao Zu
Biology 2023, 12(7), 899; https://doi.org/10.3390/biology12070899 - 23 Jun 2023
Cited by 1 | Viewed by 1828
Abstract
Mutations in HOXA1 can lead to diseases such as Bosley–Salih–Alorainy syndrome, involving severe cardiovascular malformations. However, the role of HOXA1 in cardiac morphogenesis remains unclear. hoxa1a is a homologous gene to human HOXA1 in zebrafish. We used CRISPR to make hoxa1a-null zebrafish [...] Read more.
Mutations in HOXA1 can lead to diseases such as Bosley–Salih–Alorainy syndrome, involving severe cardiovascular malformations. However, the role of HOXA1 in cardiac morphogenesis remains unclear. hoxa1a is a homologous gene to human HOXA1 in zebrafish. We used CRISPR to make hoxa1a-null zebrafish that exhibited multiple heart malformations. In situ hybridization and sections revealed the morphological changes in mutants: enlarged ventricle with thickened myocardium and increased trabeculae, intensified OFT and inadequate heart looping, with electrocardiography supporting these pathological changes. High-speed photography captured cardiac pumping and revealed a significant decrease in cardiac output. Furthermore, lacking hoxa1a led to posterior body abnormality that affected movement ability, corresponding with the motor development delay in patients. Upregulation of hox paralogues in hoxa1a-null fish implied a compensatory mechanism between hox genes. Accordingly, we successfully constructed a hoxa1a-null model with a cardiac disease pattern which occurred in human HOXA1-associated heart malformation. The study of hoxa1a in zebrafish can further promote the understanding of hox genes and related diseases. Full article
(This article belongs to the Special Issue Transgenic and Genome Editing in Fish)
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16 pages, 4816 KiB  
Article
Establishment of an Integrated CRISPR/Cas9 Plasmid System for Simple and Efficient Genome Editing in Medaka In Vitro and In Vivo
by Zeming Zhang, Jie Wang, Jianeng Li, Xiang Liu, Lei Liu, Changle Zhao, Wenjing Tao, Deshou Wang and Jing Wei
Biology 2023, 12(2), 336; https://doi.org/10.3390/biology12020336 - 20 Feb 2023
Cited by 5 | Viewed by 3255
Abstract
Although CRISPR/Cas9 has been used in gene manipulation of several fish species in vivo, its application in fish cultured cells is still challenged and limited. In this study, we established an integrated CRISPR/Cas9 plasmid system and evaluated its efficiency of gene knock-out or [...] Read more.
Although CRISPR/Cas9 has been used in gene manipulation of several fish species in vivo, its application in fish cultured cells is still challenged and limited. In this study, we established an integrated CRISPR/Cas9 plasmid system and evaluated its efficiency of gene knock-out or knock-in at a specific site in medaka (Oryzias latipes) in vitro and in vivo. By using the enhanced green fluorescent protein reporter plasmid pGNtsf1, we demonstrate that pCas9-U6sgRNA driven by endogenous U6 promoter (pCas9-mU6sgRNA) mediated very high gene editing efficiency in medaka cultured cells, but not by exogenous U6 promoters. After optimizing the conditions, the gene editing efficiencies of eight sites targeting for four endogenous genes were calculated, and the highest was up to 94% with no detectable off-target. By one-cell embryo microinjection, pCas9-mU6sgRNA also mediated efficient gene knock-out in vivo. Furthermore, pCas9-mU6sgRNA efficiently mediated gene knock-in at a specific site in medaka cultured cells as well as embryos. Collectively, our study demonstrates that the genetic relationship of U6 promoter is critical to gene editing efficiency in medaka cultured cells, and a simple and efficient system for medaka genome editing in vitro and in vivo has been established. This study provides an insight into other fish genome editing and promotes gene functional analysis. Full article
(This article belongs to the Special Issue Transgenic and Genome Editing in Fish)
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