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Biosensors
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Biosensors for Monitoring Pathogenic Agents (Volume II)
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Biosensors for Monitoring Pathogenic Agents (Volume II)

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: closed (31 May 2024) | Viewed by 4571

Special Issue Editor

Prof. Dr. Jian Wu

E-Mail Website
Guest Editor
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
Interests: application of bio-detection technology in the field of food safety; nucleic acid biosensors; nanotechnology biosensors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Pathogen detection is important in fields such as medicine, agriculture, and food science. Traditional pathogenic bacteria detection approaches involve multiple steps, including bacterial enrichment, which is time-consuming and requires professional knowledge. In order to overcome these shortcomings, biosensors have been widely exploited for the detection of pathogenic bacteria. 

At present, depending on the targets, biosensors for pathogen detection can be divided into three categories, namely, nucleic acid sensors, protein sensors, and other metabolic small-molecule sensors. Meanwhile, based on the detection principle, they can be divided into electrochemical sensors, optical sensors, and so on.  

In recent years, relevant technologies in these fields have made great progress. This Special Issue hopes to collect the latest developments in biosensor technologies for pathogen detection and to discuss the potential opportunities and challenges for their application.

Prof. Dr. Jian Wu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • pathogen detection
  • biosensors
  • electrochemical analysis
  • optical sensing

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Published Papers (2 papers)

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Research

12 pages, 4601 KiB  
Article
A Point-of-Care Nucleic Acid Quantification Method by Counting Light Spots Formed by LAMP Amplicons on a Paper Membrane
by Yanju Chen, Yuanyuan Zhu, Cheng Peng, Xiaofu Wang, Jian Wu, Huan Chen and Junfeng Xu
Biosensors 2024, 14(3), 139; https://doi.org/10.3390/bios14030139 - 10 Mar 2024
Viewed by 1819
Abstract
Nucleic acid quantification, allowing us to accurately know the copy number of target nucleic acids, is significant for diagnosis, food safety, agricultural production, and environmental protection. However, current digital quantification methods require expensive instruments or complicated microfluidic chips, making it difficult to popularize [...] Read more.
Nucleic acid quantification, allowing us to accurately know the copy number of target nucleic acids, is significant for diagnosis, food safety, agricultural production, and environmental protection. However, current digital quantification methods require expensive instruments or complicated microfluidic chips, making it difficult to popularize in the point-of-care detection. Paper is an inexpensive and readily available material. In this study, we propose a simple and cost-effective paper membrane-based digital loop-mediated isothermal amplification (LAMP) method for nucleic acid quantification. In the presence of DNA fluorescence dyes, the high background signals will cover up the amplicons-formed bright spots. To reduce the background fluorescence signals, a quencher-fluorophore duplex was introduced in LAMP primers to replace non-specific fluorescence dyes. After that, the amplicons-formed spots on the paper membrane can be observed; thus, the target DNA can be quantified by counting the spots. Take Vibrio parahaemolyticus DNA detection as an instance, a good linear relationship is obtained between the light spots and the copy numbers of DNA. The paper membrane-based digital LAMP detection can detect 100 copies target DNA per reaction within 30 min. Overall, the proposed nucleic acid quantification method has the advantages of a simple workflow, short sample-in and answer-out time, low cost, and high signal-to-noise, which is promising for application in resourced limited areas. Full article
(This article belongs to the Special Issue Biosensors for Monitoring Pathogenic Agents (Volume II))
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15 pages, 3158 KiB  
Article
Cost-Efficient Micro-Well Array-Based Colorimetric Antibiotic Susceptibility Testing (MacAST) for Bacteria from Culture or Community
by Huilin Zhang, Lei Wang, Zhiguo Zhang, Jianhan Lin and Feng Ju
Biosensors 2023, 13(12), 1028; https://doi.org/10.3390/bios13121028 - 14 Dec 2023
Viewed by 2311
Abstract
Rapid and cost-efficient antibiotic susceptibility testing (AST) is key to timely prescription-oriented diagnosis and precision treatment. However, current AST methods have limitations in throughput or cost effectiveness, and are impractical for microbial communities. Here, we developed a high-throughput micro-well array-based colorimetric AST (macAST) [...] Read more.
Rapid and cost-efficient antibiotic susceptibility testing (AST) is key to timely prescription-oriented diagnosis and precision treatment. However, current AST methods have limitations in throughput or cost effectiveness, and are impractical for microbial communities. Here, we developed a high-throughput micro-well array-based colorimetric AST (macAST) system equipped with a self-developed smartphone application that could efficiently test sixteen combinations of bacteria strains and antibiotics, achieving comparable AST results based on resazurin metabolism assay. For community samples, we integrated immunomagnetic separation into the macAST (imacAST) system to specifically enrich the target cells before testing, which shortened bacterial isolation time from days to only 45 min and achieved AST of the target bacteria with a low concentration (~103 CFU/mL). This proof-of-concept study developed a high-throughput AST system with an at least ten-fold reduction in cost compared with a system equipped with a microscope or Raman spectrum. Based on colorimetric readout, the antimicrobial susceptibility of the bacteria from microbial communities can be delivered within 6 h, compared to days being required based on standard procedures, bypassing the need for precise instrumentation in therapy to combat bacterial antibiotic resistance in resource-limited settings. Full article
(This article belongs to the Special Issue Biosensors for Monitoring Pathogenic Agents (Volume II))
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