Journal Description
Biosensors
Biosensors
is an international, peer-reviewed, open access journal on the technology and science of biosensors published monthly online by MDPI.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubMed, MEDLINE, PMC, Embase, CAPlus / SciFinder, Inspec, and other databases.
- Journal Rank: JCR - Q1 (Chemistry, Analytical) / CiteScore - Q1 (Engineering (miscellaneous))
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 18.9 days after submission; acceptance to publication is undertaken in 2.6 days (median values for papers published in this journal in the second half of 2024).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
Impact Factor:
4.9 (2023);
5-Year Impact Factor:
5.2 (2023)
Latest Articles
Biomechanical Risk Classification in Repetitive Lifting Using Multi-Sensor Electromyography Data, Revised National Institute for Occupational Safety and Health Lifting Equation, and Deep Learning
Biosensors 2025, 15(2), 84; https://doi.org/10.3390/bios15020084 (registering DOI) - 1 Feb 2025
Abstract
Repetitive lifting tasks in occupational settings often result in shoulder injuries, impacting both health and productivity. Accurately assessing the biomechanical risk of these tasks remains a significant challenge in occupational ergonomics, particularly within manufacturing environments. Traditional assessment methods frequently rely on subjective reports
[...] Read more.
Repetitive lifting tasks in occupational settings often result in shoulder injuries, impacting both health and productivity. Accurately assessing the biomechanical risk of these tasks remains a significant challenge in occupational ergonomics, particularly within manufacturing environments. Traditional assessment methods frequently rely on subjective reports and limited observations, which can introduce bias and yield incomplete evaluations. This study addresses these limitations by generating and utilizing a comprehensive dataset containing detailed time-series electromyography (EMG) data from 25 participants. Using high-precision wearable sensors, EMG data were collected from eight muscles as participants performed repetitive lifting tasks. For each task, the lifting index was calculated using the revised National Institute for Occupational Safety and Health (NIOSH) lifting equation (RNLE). Participants completed cycles of both low-risk and high-risk repetitive lifting tasks within a four-minute period, allowing for the assessment of muscle performance under realistic working conditions. This extensive dataset, comprising over 7 million data points sampled at approximately 1259 Hz, was leveraged to develop deep learning models to classify lifting risk. To provide actionable insights for practical occupational ergonomics and risk assessments, statistical features were extracted from the raw EMG data. Three deep learning models, Convolutional Neural Networks (CNNs), Multilayer Perceptron (MLP), and Long Short-Term Memory (LSTM), were employed to analyze the data and predict the occupational lifting risk level. The CNN model achieved the highest performance, with a precision of 98.92% and a recall of 98.57%, proving its effectiveness for real-time risk assessments. These findings underscore the importance of aligning model architectures with data characteristics to optimize risk management. By integrating wearable EMG sensors with deep learning models, this study enables precise, real-time, and dynamic risk assessments, significantly enhancing workplace safety protocols. This approach has the potential to improve safety planning and reduce the incidence and severity of work-related musculoskeletal disorders, ultimately promoting better health and safety outcomes across various occupational settings.
Full article
(This article belongs to the Special Issue Wearable and Implantable Bioelectronics for Advanced Biosensing and Human Health Monitoring)
►
Show Figures
Open AccessArticle
Application of Wearable Insole Sensors in In-Place Running: Estimating Lower Limb Load Using Machine Learning
by
Shipan Lang, Jun Yang, Yong Zhang, Pei Li, Xin Gou, Yuanzhu Chen, Chunbao Li and Heng Zhang
Biosensors 2025, 15(2), 83; https://doi.org/10.3390/bios15020083 (registering DOI) - 1 Feb 2025
Abstract
Musculoskeletal injuries induced by high-intensity and repetitive physical activities represent one of the primary health concerns in the fields of public fitness and sports. Musculoskeletal injuries, often resulting from unscientific training practices, are particularly prevalent, with the tibia being especially vulnerable to fatigue-related
[...] Read more.
Musculoskeletal injuries induced by high-intensity and repetitive physical activities represent one of the primary health concerns in the fields of public fitness and sports. Musculoskeletal injuries, often resulting from unscientific training practices, are particularly prevalent, with the tibia being especially vulnerable to fatigue-related damage. Current tibial load monitoring methods rely mainly on laboratory equipment and wearable devices, but datasets combining both sources are limited due to experimental complexities and signal synchronization challenges. Moreover, wearable-based algorithms often fail to capture deep signal features, hindering early detection and prevention of tibial fatigue injuries. In this study, we simultaneously collected data from laboratory equipment and wearable insole sensors during in-place running by volunteers, creating a dataset named WearLab-Leg. Based on this dataset, we developed a machine learning model integrating Temporal Convolutional Network (TCN) and Transformer modules to estimate vertical ground reaction force (vGRF) and tibia bone force (TBF) using insole pressure signals. Our model’s architecture effectively combines the advantages of local deep feature extraction and global modeling, and further introduces the Weight-MSELoss function to improve peak prediction performance. As a result, the model achieved a normalized root mean square error (NRMSE) of 7.33% for vGRF prediction and 10.64% for TBF prediction. Our dataset and proposed model offer a convenient solution for biomechanical monitoring in athletes and patients, providing reliable data and technical support for early warnings of fatigue-induced injuries.
Full article
(This article belongs to the Special Issue Wearable Sensors for Precise Exercise Monitoring and Analysis)
►▼
Show Figures
Figure 1
Open AccessArticle
Development of Electronic Nose as a Complementary Screening Tool for Breath Testing in Colorectal Cancer
by
Chih-Dao Chen, Yong-Xiang Zheng, Heng-Fu Lin and Hsiao-Yu Yang
Biosensors 2025, 15(2), 82; https://doi.org/10.3390/bios15020082 (registering DOI) - 1 Feb 2025
Abstract
(1) Background: Colorectal cancer is one of the leading causes of cancer-related death, while early detection decreases incidence and mortality. Current screening programs involving fecal immunological testing and colonoscopy commonly bring about unnecessary colonoscopies, which adds burden to healthcare systems. The objective of
[...] Read more.
(1) Background: Colorectal cancer is one of the leading causes of cancer-related death, while early detection decreases incidence and mortality. Current screening programs involving fecal immunological testing and colonoscopy commonly bring about unnecessary colonoscopies, which adds burden to healthcare systems. The objective of this study was to provide an assessment of the diagnostic performance of an electronic nose serving as a complementary screening tool to improve current screening programs in clinical settings. (2) Methods: We conducted a case–control study that included patients from a medical center with colorectal cancer and non-colorectal cancer controls. We analyzed the composition of volatile organic compounds in their exhaled breath using the electronic nose. We then used machine learning algorithms to develop predictive models and provided the estimated accuracy and reliability of the breath testing. (3) Results: We enrolled 77 patients, with 40 cases and 37 controls. The area under the curve, Kappa coefficient, sensitivity, and specificity of the selected model were 0.87 (95% CI 0.76–0.95), 0.66 (95% CI 0.49–0.83), 0.81, and 0.85. For subjects at an early stage of disease, the sensitivity and specificity were 0.90 and 0.85. Excluding smokers, the sensitivity and specificity were 0.88 and 0.92. (4) Conclusions: This study highlights the promising potential of breath testing using an electronic nose for enabling early detection and reducing unnecessary treatments. However, more independent data for external validation are required to ensure applicability and generalizability.
Full article
(This article belongs to the Collection Novel Sensing System for Biomedical Applications)
►▼
Show Figures
Figure 1
Open AccessArticle
An Innovative Enzymatic Surface Plasmon Resonance-Based Biosensor Designed for Precise Detection of Glycine Amino Acid
by
Gabriela Elizabeth Quintanilla-Villanueva, Osvaldo Rodríguez-Quiroz, Araceli Sánchez-Álvarez, José Manuel Rodríguez-Delgado, Juan Francisco Villarreal-Chiu, Donato Luna-Moreno and Melissa Marlene Rodríguez-Delgado
Biosensors 2025, 15(2), 81; https://doi.org/10.3390/bios15020081 (registering DOI) - 1 Feb 2025
Abstract
Glycine is an essential amino acid involved in synthesizing a variety of important biomolecules, and its concentration can influence numerous biochemical processes, including the severity of symptoms in a wide range of conditions in humans, such as cancer, schizophrenia, major depression, and diabetes.
[...] Read more.
Glycine is an essential amino acid involved in synthesizing a variety of important biomolecules, and its concentration can influence numerous biochemical processes, including the severity of symptoms in a wide range of conditions in humans, such as cancer, schizophrenia, major depression, and diabetes. While a few costly or labour-intensive methods are currently available, we have developed a new enzymatic biosensor that can accurately measure glycine levels with remarkable simplicity. By employing immobilized laccase enzymes in combination with a surface plasmon resonance (SPR) device, our system achieved a limit of detection (LOD) of 9.95 mM and a limit of quantification (LOQ) of 33.19 mM. In addition, it demonstrated a recovery rate of 97.64 ± 7.71%. Moreover, the biosensor maintained consistent signal intensity over 21 days and supported a total of 60 analyses using the same immobilized enzyme setup, demonstrating excellent reusability. Notably, this study marks the first time glycine has been determined using an enzymatic SPR-based platform.
Full article
(This article belongs to the Special Issue Biosensor Nanoengineering: Design, Operation and Implementation—2nd Edition)
►▼
Show Figures
Figure 1
Open AccessArticle
A Dual Nano-Signal Probe-Based Electrochemical Immunosensor for the Simultaneous Detection of Two Biomarkers in Gastric Cancer
by
Li-Ting Su, Zhen-Qing Yang, Hua-Ping Peng and Ai-Lin Liu
Biosensors 2025, 15(2), 80; https://doi.org/10.3390/bios15020080 (registering DOI) - 31 Jan 2025
Abstract
Detecting multiple tumor markers is of great importance. It helps in early cancer detection, accurate diagnosis, and monitoring treatment. In this work, gold nanoparticles–toluidine blue–graphene oxide (AuNPs-TB–GO) and gold nanoparticles–carboxyl ferrocene–tungsten disulfide (AuNPs–FMC–WS2) nanocomposites were prepared for labeling Carcinoembryonic antigen (CEA)
[...] Read more.
Detecting multiple tumor markers is of great importance. It helps in early cancer detection, accurate diagnosis, and monitoring treatment. In this work, gold nanoparticles–toluidine blue–graphene oxide (AuNPs-TB–GO) and gold nanoparticles–carboxyl ferrocene–tungsten disulfide (AuNPs–FMC–WS2) nanocomposites were prepared for labeling Carcinoembryonic antigen (CEA) antibody and Carbohydrate antigen 72–4 (CA72-4) antibody, respectively, and used as two kinds of probes with different electrochemical signals. With the excellent magnetic performance of biotin immune magnetic beads (IMBs), the biofunctional IMBs were firmly deposited on the magnetic glassy carbon electrode (MGCE) surface by applying a constant magnetic field, and then the CEA and CA72-4 antibody were immobilized on the IMBs by the avidin–biotin conjugation. The assay was based on the change in the detection peak current. Under the optimum experimental conditions, the linear range of detection of CEA is of the two-component immunosensor is from 0.01 to 120 ng/mL, with a low detection limit of 0.003 ng/mL, and the linear range of detection of CA72-4 is from 0.05 to 35 U/mL, with a detection limit of 0.016 U/mL. The results showed that the proposed immunosensor enabled simultaneous monitoring of CEA and CA72-4 and exhibited good reproducibility, excellent high selectivity, and sensitivity. In particular, the proposed multiplexed immunoassay approach does not require sophisticated fabrication and is well-suited for high-throughput biosensing and application to other areas.
Full article
(This article belongs to the Special Issue Trends and Perspective of Advanced Nanotechnology for Bio-Sensing, Imaging and Cancer Therapy)
►▼
Show Figures
Figure 1
Open AccessArticle
On-Chip Polarization Light Microscopy
by
Túlio de L. Pedrosa, Renato E. de Araujo and Sebastian Wachsmann-Hogiu
Biosensors 2025, 15(2), 79; https://doi.org/10.3390/bios15020079 - 30 Jan 2025
Abstract
Polarization light microscopy (PLM) enables detailed examination of birefringent materials and reveals unique features that cannot be observed under non-polarized light. Implementation of this technique for quantitative PLM (QPLM) assessment of samples is challenging and requires specialized components and equipment. Here, we demonstrate
[...] Read more.
Polarization light microscopy (PLM) enables detailed examination of birefringent materials and reveals unique features that cannot be observed under non-polarized light. Implementation of this technique for quantitative PLM (QPLM) assessment of samples is challenging and requires specialized components and equipment. Here, we demonstrate QPLM on a semiconductor imaging chip that is suitable for point-of-care/need applications. A white LED illumination was used with crossed polarizers and a full wave plate to perform on-chip, non-contact-mode QPLM. Polarization complexity is probed by assessing the multispectral phase shift experienced by white light through the distinct optical paths of the sample. This platform can achieve micrometer-scale spatial resolution with a Field of View determined by the size of the semiconductor sensor. Visualization of a biological sample (Euglena gracilis) was demonstrated, as well as the detection of Monosodium Urate crystals, where the presence of negative birefringence of crystals in synovial fluid is important for the diagnosis of gout.
Full article
(This article belongs to the Special Issue Advanced Optical Methods for Biosensing)
Open AccessArticle
Detection of Cognitive Performance Deterioration Due to Cold-Air Exposure in Females Using Wearable Electrodermal Activity and Electrocardiogram
by
Youngsun Kong, Riley McNaboe, Md Billal Hossain, Hugo F. Posada-Quintero, Krystina Diaz, Ki H. Chon and Jeffrey Bolkhovsky
Biosensors 2025, 15(2), 78; https://doi.org/10.3390/bios15020078 - 29 Jan 2025
Abstract
Prolonged exposure to cold air can impair reaction time and cognitive function, which can lead to serious consequences. One mitigation strategy is to develop models that can predict cognitive performance by tracking physiological metrics associated with cold stress. As females are evidenced to
[...] Read more.
Prolonged exposure to cold air can impair reaction time and cognitive function, which can lead to serious consequences. One mitigation strategy is to develop models that can predict cognitive performance by tracking physiological metrics associated with cold stress. As females are evidenced to be more sensitive to cold exposure, this study investigated the relationship between physiological metrics and cognitive performance deterioration of female subjects under cold stress. Wearable electrodermal activity (EDA) and electrocardiogram (ECG) were collected from nineteen females who underwent five sessions of a cognitive task battery—assessing reaction time, memory, and attention—in a cold (10 °C) environment. Machine learning classifiers showed higher cognitive performance classification accuracies with heart rate variability (HRV) features than with EDA features. Particularly in detecting performance deterioration in a task associated with assessing short-term memory, our support vector machine classifier with HRV features showed an 82.4% accuracy, with a sensitivity of 84.2% and a specificity of 80.6%, whereas a 55.4% accuracy with a sensitivity of 44.7% and a specificity of 66.7% was obtained with EDA features. Our results demonstrate the feasibility of detecting performance deterioration from females who underwent cold exposure using wearable EDA and ECG, allowing for preventive measures to reduce risk in cold environments, especially for female military personnel.
Full article
(This article belongs to the Special Issue Advances in Flexible Bioelectronics and Intelligent Biosensing Systems)
►▼
Show Figures
Figure 1
Open AccessArticle
Enzyme Biosensor Based on 3D-Printed Flow-Through Reactor Modified with Thiacalixarene-Functionalized Oligo (Lactic Acids)
by
Dmitry Stoikov, Dominika Kappo, Alexey Ivanov, Vladimir Gorbachuk, Olga Mostovaya, Pavel Padnya, Ivan Stoikov and Gennady Evtugyn
Biosensors 2025, 15(2), 77; https://doi.org/10.3390/bios15020077 - 29 Jan 2025
Abstract
Electrochemical enzyme biosensors are extensively utilized in clinical analysis and environmental monitoring, yet achieving effective enzyme immobilization while maintaining high activity remains a challenge. In this work, we developed a flow-through enzyme biosensor system using a 3D-printed flow-through electrochemical cell fabricated from commercially
[...] Read more.
Electrochemical enzyme biosensors are extensively utilized in clinical analysis and environmental monitoring, yet achieving effective enzyme immobilization while maintaining high activity remains a challenge. In this work, we developed a flow-through enzyme biosensor system using a 3D-printed flow-through electrochemical cell fabricated from commercially available poly (lactic acid). After modification with thiacalixarene-functionalized oligo (lactic acids) (OLAs), the material enabled efficient immobilization of uricase on the inner surface of a replaceable reactor of the cell. Swelling and hydrolytic stability of OLAs in cone, partial cone, and 1,3-alternate conformations were studied, with 1,3-alernate conformation demonstrating superior stability and enzyme immobilization performance. The use of OLAs enhanced immobilization efficiency by over 30% and protected the reactor from swelling, hydrolytic degradation, and enzyme loss. The biosensor was validated for amperometric uric acid determination, with a screen-printed carbon electrode modified with carbon black and Prussian Blue. This modification reduced the cathodic potential for uric acid detection to –0.05 V. The biosensor exhibited a linear detection range of 10 nM to 30 μM with a detection limit of 7 nM, and it performed effectively in artificial urine and synthetic blood plasma. The novel cell design, featuring easy assembly and low-cost replaceable parts, makes this biosensor a promising candidate for routine clinical analysis and other practical applications.
Full article
(This article belongs to the Special Issue Feature Paper in Biosensor and Bioelectronic Devices 2024)
Open AccessReview
Recent Progress in PDMS-Based Microfluidics Toward Integrated Organ-on-a-Chip Biosensors and Personalized Medicine
by
Fahad Alghannam, Mrwan Alayed, Salman Alfihed, Mahmoud A. Sakr, Dhaifallah Almutairi, Naif Alshamrani and Nojoud Al Fayez
Biosensors 2025, 15(2), 76; https://doi.org/10.3390/bios15020076 - 29 Jan 2025
Abstract
The organ-on-a-chip (OoC) technology holds significant promise for biosensors and personalized medicine by enabling the creation of miniature, patient-specific models of human organs. This review studies the recent advancements in the application of polydimethylsiloxane (PDMS) microfluidics for OoC purposes. It underscores the main
[...] Read more.
The organ-on-a-chip (OoC) technology holds significant promise for biosensors and personalized medicine by enabling the creation of miniature, patient-specific models of human organs. This review studies the recent advancements in the application of polydimethylsiloxane (PDMS) microfluidics for OoC purposes. It underscores the main fabrication technologies of PDMS microfluidic systems, such as photolithography, injection molding, hot embossing, and 3D printing. The review also highlights the crucial role of integrated biosensors within OoC platforms. These electrochemical, electrical, and optical sensors, integrated within the microfluidic environment, provide valuable insights into cellular behavior and drug response. Furthermore, the review explores the exciting potential of PDMS-based OoC technology for personalized medicine. OoC devices can forecast drug effectiveness and tailor therapeutic strategies for patients by incorporating patient-derived cells and replicating individual physiological variations, helping the healing process and accelerating recovery. This personalized approach can revolutionize healthcare by offering more precise and efficient treatment options. Understanding OoC fabrication and its applications in biosensors and personalized medicine can play a pivotal role in future implementations of multifunctional OoC biosensors.
Full article
(This article belongs to the Special Issue Microfluidic Chips for Life Science and Health Care Applications)
Open AccessArticle
Artificial-Intelligence Bio-Inspired Peptide for Salivary Detection of SARS-CoV-2 in Electrochemical Biosensor Integrated with Machine Learning Algorithms
by
Marcelo Augusto Garcia-Junior, Bruno Silva Andrade, Ana Paula Lima, Iara Pereira Soares, Ana Flávia Oliveira Notário, Sttephany Silva Bernardino, Marco Fidel Guevara-Vega, Ghabriel Honório-Silva, Rodrigo Alejandro Abarza Munoz, Ana Carolina Gomes Jardim, Mário Machado Martins, Luiz Ricardo Goulart, Thulio Marquez Cunha, Murillo Guimarães Carneiro and Robinson Sabino-Silva
Biosensors 2025, 15(2), 75; https://doi.org/10.3390/bios15020075 - 28 Jan 2025
Abstract
Developing affordable, rapid, and accurate biosensors is essential for SARS-CoV-2 surveillance and early detection. We created a bio-inspired peptide, using the SAGAPEP AI platform, for COVID-19 salivary diagnostics via a portable electrochemical device coupled to Machine Learning algorithms. SAGAPEP enabled molecular docking simulations
[...] Read more.
Developing affordable, rapid, and accurate biosensors is essential for SARS-CoV-2 surveillance and early detection. We created a bio-inspired peptide, using the SAGAPEP AI platform, for COVID-19 salivary diagnostics via a portable electrochemical device coupled to Machine Learning algorithms. SAGAPEP enabled molecular docking simulations against the SARS-CoV-2 Spike protein’s RBD, leading to the synthesis of Bio-Inspired Artificial Intelligence Peptide 1 (BIAI1). Molecular docking was used to confirm interactions between BIAI1 and SARS-CoV-2, and BIAI1 was functionalized on rhodamine-modified electrodes. Cyclic voltammetry (CV) using a [Fe(CN)6]3−/4 solution detected virus levels in saliva samples with and without SARS-CoV-2. Support vector machine (SVM)-based machine learning analyzed electrochemical data, enhancing sensitivity and specificity. Molecular docking revealed stable hydrogen bonds and electrostatic interactions with RBD, showing an average affinity of –250 kcal/mol. Our biosensor achieved 100% sensitivity, 80% specificity, and 90% accuracy for 1.8 × 10⁴ focus-forming units in infected saliva. Validation with COVID-19-positive and -negative samples using a neural network showed 90% sensitivity, specificity, and accuracy. This BIAI1-based electrochemical biosensor, integrated with machine learning, demonstrates a promising non-invasive, portable solution for COVID-19 screening and detection in saliva.
Full article
(This article belongs to the Special Issue Electrochemical (Bio-) Sensors in Biological Applications—2nd Edition)
Open AccessReview
Dual Biomarker Strategies for Liquid Biopsy: Integrating Circulating Tumor Cells and Circulating Tumor DNA for Enhanced Tumor Monitoring
by
Ga Young Moon, Basak Dalkiran, Hyun Sung Park, Dongjun Shin, Chaeyeon Son, Jung Hyun Choi, Seha Bang, Hosu Lee, Il Doh, Dong Hyung Kim, Woo-jin Jeong and Jiyoon Bu
Biosensors 2025, 15(2), 74; https://doi.org/10.3390/bios15020074 - 28 Jan 2025
Abstract
The liquid biopsy has gained significant attention in cancer diagnostics, with circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) being recognized as key biomarkers for tumor detection and monitoring. However, each biomarker possesses inherent limitations that restrict its standalone clinical utility, such
[...] Read more.
The liquid biopsy has gained significant attention in cancer diagnostics, with circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) being recognized as key biomarkers for tumor detection and monitoring. However, each biomarker possesses inherent limitations that restrict its standalone clinical utility, such as the rarity and heterogeneity of CTCs and the variable sensitivity and specificity of ctDNA assays. This highlights the necessity of integrating both biomarkers to maximize diagnostic and prognostic potential, offering a more comprehensive understanding of the tumor biology and therapeutic response. In this review, we summarize clinical studies that have explored the combined analysis of CTCs and ctDNA as biomarkers, providing insights into their synergistic value in diverse tumor types. Specifically, this paper examines the individual advantages and limitations of CTCs and ctDNA, details the findings of combined biomarker studies across various cancers, highlights the benefits of dual biomarker approaches over single-biomarker strategies, and discusses future prospects for advancing personalized oncology through liquid biopsies. By offering a comprehensive overview of clinical studies combining CTCs and ctDNA, this review serves as a guideline for researchers and clinicians aiming to enhance biomarker-based strategies in oncology and informs biosensor design for improved biomarker detection.
Full article
(This article belongs to the Special Issue Immunoassays and Biosensing (2nd Edition))
Open AccessReview
Sensors and Devices Based on Electrochemical Skin Conductance and Bioimpedance Measurements for the Screening of Diabetic Foot Syndrome: Review and Meta-Analysis
by
Federica Verdini, Alessandro Mengarelli, Gaetano Chemello, Benedetta Salvatori, Micaela Morettini, Christian Göbl and Andrea Tura
Biosensors 2025, 15(2), 73; https://doi.org/10.3390/bios15020073 - 26 Jan 2025
Abstract
Diabetic foot syndrome is a multifactorial disease involving different etiological factors. This syndrome is also insidious, due to frequent lack of early symptoms, and its prevalence has increased in recent years. This justifies the remarkable attention being paid to the syndrome, although the
[...] Read more.
Diabetic foot syndrome is a multifactorial disease involving different etiological factors. This syndrome is also insidious, due to frequent lack of early symptoms, and its prevalence has increased in recent years. This justifies the remarkable attention being paid to the syndrome, although the problem of effective early screening for this syndrome, possibly at a patient’s home, is still unsolved. However, some options appear available in this context. First, it was demonstrated that the temperature measurement of the foot skin is an interesting approach, but it also has some limitations, and hence a more effective approach should combine data from temperature and from other sensors. For this purpose, foot skin conductance or bioimpedance measurement may be a good option. Therefore, the aim of this study was to review those studies where skin conductance/bioimpedance measurement was used for the detection of diabetic foot syndrome. In addition, we performed a meta-analysis of some of those studies, where a widely used device was exploited (SUDOSCAN®) for foot skin conductance measurement, and we found that skin conductance levels can clearly distinguish between groups of patients with and without diabetic neuropathy, the latter being one of the most relevant factors in diabetic foot syndrome.
Full article
(This article belongs to the Special Issue Advanced Bioelectronics for Healthcare Monitoring and Disease Diagnosis)
►▼
Show Figures
Figure 1
Open AccessReview
Metal Nanocluster-Based Biosensors for DNA Detection
by
Ran He, Sheng Wang, Feiye Ju, Zhao Huang, Yuan Gao, Jing Zhang, Nongyue He and Libo Nie
Biosensors 2025, 15(2), 72; https://doi.org/10.3390/bios15020072 - 25 Jan 2025
Abstract
The early detection of genetic diseases is a critical need in modern medicine, underscoring the importance of developing deoxyribonucleic acid (DNA) biosensors. In recent years, metal nanoclusters (MNCs) have demonstrated significant potential as biosensors for DNA detection due to their ultra-small size, excellent
[...] Read more.
The early detection of genetic diseases is a critical need in modern medicine, underscoring the importance of developing deoxyribonucleic acid (DNA) biosensors. In recent years, metal nanoclusters (MNCs) have demonstrated significant potential as biosensors for DNA detection due to their ultra-small size, excellent photostability, bright photoluminescence, low toxicity and other outstanding properties. This review firstly discusses the characteristics of MNCs, which are effective in the early diagnosis of DNA diseases. Subsequently, different synthesis methods of MNCs are introduced. In the following section, DNA sensors based on different types of MNCs and their respective detection mechanisms are discussed in detail. Finally, the opportunities and challenges faced by DNA sensors based on MNCs are analyzed.
Full article
(This article belongs to the Special Issue Materials and Techniques for Bioanalysis and Biosensing—2nd Edition)
►▼
Show Figures
Graphical abstract
Open AccessArticle
DNA-Based Nanobiosensor for the Colorimetric Detection of Dengue Virus Serotype 2 Synthetic Target Oligonucleotide
by
Michael Sandino C. Flores, Evangelyn C. Alocilja, Divina M. Amalin, Mae Joanne B. Aguila, Marynold V. Purificacion, Florinia E. Merca, Ma. Carmina C. Manuel, Mark Pierre S. Dimamay, Ma. Anita M. Bautista and Lilia M. Fernando
Biosensors 2025, 15(2), 71; https://doi.org/10.3390/bios15020071 - 24 Jan 2025
Abstract
Annually, the Philippines is burdened by a high number of infections and deaths due to Dengue. This disease is caused by the Dengue virus (DENV) and is transmitted from one human host to another by the female Aedes aegypti mosquito. Being a developing
[...] Read more.
Annually, the Philippines is burdened by a high number of infections and deaths due to Dengue. This disease is caused by the Dengue virus (DENV) and is transmitted from one human host to another by the female Aedes aegypti mosquito. Being a developing country, most of the high-risk areas in the Philippines are resource-limited and cannot afford equipment for detection and monitoring. Moreover, traditional clinical diagnoses of DENV infection are costly and time-consuming and require expertise. Hence, it is important to establish effective vector control and surveillance measures. In this study, we developed a DNA-based nanobiosensor for the colorimetric detection of Dengue virus serotype 2 (DENV-2) synthetic target DNA (stDNA S2) using gold nanoparticles (AuNPs). We successfully functionalized dextrin-capped gold nanoparticles with the designed DENV-2 oligonucleotide probes. The detection of the complementary stDNA S2, indicated by the pink-colored solution, was successfully performed within 15 min using 0.40 M NaCl solution. We were able to detect up to 36.14 ng/μL of stDNA S2 with some cross-reactivity observed with one non-complementary target. We believe that our study offers a basis for developing nanobiosensors for other DENV serotypes.
Full article
(This article belongs to the Section Biosensor and Bioelectronic Devices)
►▼
Show Figures
Figure 1
Open AccessArticle
Shared Control of Supernumerary Robotic Limbs Using Mixed Realityand Mouth-and-Tongue Interfaces
by
Hongwei Jing, Sikai Zhao, Tianjiao Zheng, Lele Li, Qinghua Zhang, Kerui Sun, Jie Zhao and Yanhe Zhu
Biosensors 2025, 15(2), 70; https://doi.org/10.3390/bios15020070 - 23 Jan 2025
Abstract
Supernumerary Robotic Limbs (SRLs) are designed to collaborate with the wearer, enhancing operational capabilities. When human limbs are occupied with primary tasks, controlling SRLs flexibly and naturally becomes a challenge. Existing methods such as electromyography (EMG) control and redundant limb control partially address
[...] Read more.
Supernumerary Robotic Limbs (SRLs) are designed to collaborate with the wearer, enhancing operational capabilities. When human limbs are occupied with primary tasks, controlling SRLs flexibly and naturally becomes a challenge. Existing methods such as electromyography (EMG) control and redundant limb control partially address SRL control issues. However, they still face limitations like restricted degrees of freedom and complex data requirements, which hinder their applicability in real-world scenarios. Additionally, fully autonomous control methods, while efficient, often lack the flexibility needed for complex tasks, as they do not allow for real-time user adjustments. In contrast, shared control combines machine autonomy with human input, enabling finer control and more intuitive task completion. Building on our previous work with the mouth-and-tongue interface, this paper integrates a mixed reality (MR) device to form an interactive system that enables shared control of the SRL. The system allows users to dynamically switch between voluntary and autonomous control, providing both flexibility and efficiency. A random forest model classifies 14 distinct tongue and mouth operations, mapping them to six-degree-of-freedom SRL control. In comparative experiments involving ten healthy subjects performing assembly tasks under three control modes (shared control, autonomous control, and voluntary control), shared control demonstrates a balance between machine autonomy and human input. While autonomous control offers higher task efficiency, shared control achieves greater task success rates and improves user experience by combining the advantages of both autonomous operation and voluntary control. This study validates the feasibility of shared control and highlights its advantages in providing flexible switching between autonomy and user intervention, offering new insights into SRL control.
Full article
(This article belongs to the Section Wearable Biosensors)
►▼
Show Figures
Figure 1
Open AccessArticle
Sustainable and Flexible Surface-Enhanced Raman Scattering Transducer: Gold Nanoparticle-Bacterial Cellulose Composite for Pesticide Monitoring in Agrifood Systems
by
Daniela Lospinoso, Adriano Colombelli, Sudipto Pal, Pasquale Cretì, Maria Concetta Martucci, Gabriele Giancane, Antonio Licciulli, Roberto Rella and Maria Grazia Manera
Biosensors 2025, 15(2), 69; https://doi.org/10.3390/bios15020069 - 23 Jan 2025
Abstract
Functionalized plasmonic nanostructure platforms are widely used for developing optical biosensors and SERS assays. In this work, we present a low-cost and scalable surface-enhanced Raman scattering (SERS) system based on an innovative optical transducer comprising gold nanoparticles (AuNPs) embedded in nano-fibrillated bacterial cellulose
[...] Read more.
Functionalized plasmonic nanostructure platforms are widely used for developing optical biosensors and SERS assays. In this work, we present a low-cost and scalable surface-enhanced Raman scattering (SERS) system based on an innovative optical transducer comprising gold nanoparticles (AuNPs) embedded in nano-fibrillated bacterial cellulose (BC). The AuNPs@BC composite leverages the unique nanofibrillar architecture of bacterial cellulose, which provides a high surface area, flexibility, and uniform nanoparticle distribution, enabling the formation of numerous electromagnetic “hot spots”. This structure excites localized surface plasmon resonance (LSPR), as demonstrated by a bulk sensitivity of 72 nm/RIU, and supports enhanced Raman signal amplification. The eco-friendly and disposable AuNPs@BC platform was tested for agrifood applications, focusing on the detection of thiram pesticide. The system achieved a detection limit of 0.24 ppm (1 µM), meeting the sensitivity requirements for regulatory compliance in food safety. A strong linear correlation (R2 ≈ 0.99) was observed between the SERS peak intensity at 1370 cm−1 and thiram concentrations, underscoring its potential for quantitative analysis. The combination of high sensitivity, reproducibility, and environmental sustainability makes the AuNPs@BC platform a promising solution for developing cost-effective, flexible, and portable sensors for pesticide monitoring and other biosensing applications.
Full article
(This article belongs to the Special Issue Ultrasensitive Biosensors and Bioassays for Real-Time Monitoring of Food Contaminants)
►▼
Show Figures
Figure 1
Open AccessReview
Beyond Traditional Lateral Flow Assays: Enhancing Performance Through Multianalytical Strategies
by
Eleni Lamprou, Panagiota M. Kalligosfyri and Despina P. Kalogianni
Biosensors 2025, 15(2), 68; https://doi.org/10.3390/bios15020068 - 23 Jan 2025
Abstract
Multiplex lateral flow assays are one of the greatest advancements in the world of rapid diagnostics, achieving the performance of several tests in one. These tests meet the basic requirements of increasing ease of use, low detection limit, and high specificity, as they
[...] Read more.
Multiplex lateral flow assays are one of the greatest advancements in the world of rapid diagnostics, achieving the performance of several tests in one. These tests meet the basic requirements of increasing ease of use, low detection limit, and high specificity, as they combine the use of novel strategies, such as the exploitation of multiple detection labels, and a variety of amplification methods. These tests have proven their usefulness in many different areas, including clinical diagnostics, food, and environmental monitoring. In this review paper, we attempt to highlight and discuss the predominant changes in multianalyte LFAs, as related to their principle, their development, and their combination with other methods. Attention is paid to their flexibility and the challenges associated with the use of LFA arrays, including strategies to improve the detectability, sensitivity, and reliability of the assays. Therefore, this review emphasizes the current advances in the field to underline the possible impact of multiplex LFAs on the future of diagnostics and analytical sciences.
Full article
(This article belongs to the Special Issue Recent Advances and Applications of Multiplexed Analysis and Multiplexed Nanobiosensors)
►▼
Show Figures
Graphical abstract
Open AccessArticle
Development and Optimization of a Cost-Effective Electrochemical Immunosensor for Rapid COVID-19 Diagnosis
by
Thaís Machado Lima, Daiane Martins Leal, Zirlane Coelho Ferreira, Fernando de Jesus Souza, Danilo Bretas de Oliveira, Etel Rocha-Vieira, Helen Rodrigues Martins, Arnaldo César Pereira and Lucas Franco Ferreira
Biosensors 2025, 15(2), 67; https://doi.org/10.3390/bios15020067 - 22 Jan 2025
Abstract
The coronavirus disease (COVID-19) pandemic has created an urgent need for rapid, accurate, and cost-effective diagnostic tools. In this study, an economical electrochemical immunosensor for the rapid diagnosis of COVID-19 was developed and optimized based on charge transfer resistance (Rct) values obtained by
[...] Read more.
The coronavirus disease (COVID-19) pandemic has created an urgent need for rapid, accurate, and cost-effective diagnostic tools. In this study, an economical electrochemical immunosensor for the rapid diagnosis of COVID-19 was developed and optimized based on charge transfer resistance (Rct) values obtained by electrochemical impedance spectroscopy (EIS) from the interaction between antibodies (anti-SARS-CoV-2) immobilized as a bioreceptor and the virus (SARS-CoV-2). The sensor uses modified pencil graphite electrodes (PGE) coated with poly(4-hydroxybenzoic acid), anti-SARS-CoV-2, and silver nanoparticles. The immobilization of anti-SARS-CoV-2 antibodies was optimized at a concentration of 1:250 for 30 min, followed by blocking the surface with 0.01% bovine serum albumin for 10 min. The optimal conditions for virus detection in clinical samples were a 1:10 dilution with a response time of 20 min. The immunosensor responded linearly in the range of 0.2–2.5 × 106 particles/μL. From the relationship between the obtained signal and the concentration of the analyzed sample, the limit of detection (LOD) and limit of quantification (LOQ) obtained were 1.21 × 106 and 4.04 × 106 particles/μL, respectively. The device did not cross-react with other viruses, including Influenza A and B, HIV, and Vaccinia virus. The relative standard deviation (RSD) of the six immunosensors prepared using the shared-pool sample was 3.87. Decreases of 22.3% and 12.4% were observed in the response values of the ten immunosensors stored at 25 °C and 4.0 °C, respectively. The sensor provides timely and accurate results with high sensitivity and specificity, offering a cost-effective alternative to the existing diagnostic methods.
Full article
(This article belongs to the Special Issue Nanomaterial-Enhanced Biosensing for Point-of-Care Diagnostics)
►▼
Show Figures
Figure 1
Open AccessArticle
Focal Molography Allows for Affinity and Concentration Measurements of Proteins in Complex Matrices with High Accuracy
by
Lorin Dirscherl, Laura S. Merz, Ronya Kobras, Peter Spies, Andreas Frutiger, Volker Gatterdam and Dominik M. Meinel
Biosensors 2025, 15(2), 66; https://doi.org/10.3390/bios15020066 - 22 Jan 2025
Abstract
Characterizing biomolecular receptor–ligand interactions is critical for research and development. However, performing analyses in complex, biologically relevant matrices, such as serum, remains challenging due to non-specific binding that often impairs measurements. Here, we evaluated Focal Molography (FM) for determining KD and kinetic
[...] Read more.
Characterizing biomolecular receptor–ligand interactions is critical for research and development. However, performing analyses in complex, biologically relevant matrices, such as serum, remains challenging due to non-specific binding that often impairs measurements. Here, we evaluated Focal Molography (FM) for determining KD and kinetic constants in comparison to gold-standard methods using single-domain heavy-chain antibodies in various systems. FM provided kinetic constants highly comparable to SPR and BLI in standard buffers containing blocking proteins, with KDs of soluble CD4 (sCD4) interactions within a 2.4-fold range across technologies. In buffers lacking blocking proteins, FM demonstrated greater robustness against non-specific binding and rebinding effects. In serum, FM exhibited stable baseline signals, unlike SPR and BLI, and yielded KDs of sCD4 interaction in 50% Bovine Serum within a 1.8-fold range of those obtained in standard buffers. For challenging molecules prone to non-specific binding (Granzyme B), FM successfully determined kinetic constants without external referencing. Finally, FM enabled direct analyte quantification in complex matrices. sCD4 quantification in cell culture media and 50% FBS showed recovery rates of 97.8–100.3% with an inter-assay CV below 1.3%. This study demonstrates the high potential of FM for kinetic affinity determination and biomarker quantification in complex matrices, enabling reliable measurements under biologically relevant conditions.
Full article
(This article belongs to the Special Issue Emerging Applications of Label-Free Optical Biosensors)
►▼
Show Figures
Figure 1
Open AccessArticle
A Time-Resolved Fluorescent Microsphere Immunochromatographic Assay for Determination of Vitamin B12 in Infant Formula Milk Powder
by
Qianqian Lu, Yongwei Feng, Qi Zhou, Ting Yang, Hua Kuang, Chuanlai Xu and Lingling Guo
Biosensors 2025, 15(2), 65; https://doi.org/10.3390/bios15020065 - 21 Jan 2025
Abstract
Vitamin B12 (VB12) is an important nutrient, and its quality control in food is crucial. In this study, based on the principle of specific recognition of target analyte by monoclonal antibodies (mAbs), a time-resolved fluorescent microsphere immunochromatographic assay (TRFM-ICA) was developed to detect
[...] Read more.
Vitamin B12 (VB12) is an important nutrient, and its quality control in food is crucial. In this study, based on the principle of specific recognition of target analyte by monoclonal antibodies (mAbs), a time-resolved fluorescent microsphere immunochromatographic assay (TRFM-ICA) was developed to detect the content of VB12 in infant formula milk powder. First, the performance of the anti-VB12 mAb was evaluated, revealing a half-maximal inhibitory concentration of 0.370 ng/mL, an affinity constant of 2.604 × 109 L/mol and no cross-reactivity with other vitamins. Then, a highly sensitive TRFM-ICA was developed, with a visual limit of detection of 10 g/kg and a cut-off value of 100 g/kg for qualitative detection and a detection range of 4.125–82.397 g/kg for quantitative detection. In addition, the test results of real samples were consistent with the results of quantification using microbiological methods, with a coefficient of variation of less than 10%, showing good accuracy and stability, and confirming that the TRFM-ICA is suitable for the analysis of VB12 in real infant formula milk powder samples. In this study, based on the principle of specific recognition of VB12 by monoclonal antibodies (mAbs) against VB12, a time-resolved fluorescence microsphere immunochromatographic assay (TRFM-ICA) was developed to detect the content of VB12 in infant formula by converting biological signals into optical signals.
Full article
(This article belongs to the Special Issue Feature Papers of Biosensors)
►▼
Show Figures
Figure 1
Journal Menu
► ▼ Journal Menu-
- Biosensors Home
- Aims & Scope
- Editorial Board
- Reviewer Board
- Topical Advisory Panel
- Instructions for Authors
- Special Issues
- Topics
- Sections & Collections
- Article Processing Charge
- Indexing & Archiving
- Editor’s Choice Articles
- Most Cited & Viewed
- Journal Statistics
- Journal History
- Journal Awards
- Conferences
- Editorial Office
Journal Browser
► ▼ Journal BrowserHighly Accessed Articles
Latest Books
E-Mail Alert
News
Topics
Topic in
Applied Sciences, Biosensors, Chips, Micromachines, Molecules
Advances in Microfluidics and Lab on a Chip Technology, 2nd Edition
Topic Editors: Roman Grzegorz Szafran, Yi YangDeadline: 31 August 2025
Topic in
Applied Sciences, Biosensors, Designs, Electronics, Materials, Micromachines
Wearable Bioelectronics: The Next Generation of Health Insights
Topic Editors: Shuo Gao, Yu Wu, Wenyu WangDeadline: 31 March 2026
Conferences
Special Issues
Special Issue in
Biosensors
Sensors for Human Activity Recognition: 3rd Edition
Guest Editors: Hui Liu, Tanja Schultz, Hugo GamboaDeadline: 10 February 2025
Special Issue in
Biosensors
Microfluidic Biosensors: Advances and Applications
Guest Editor: Yu-Chih ChenDeadline: 25 February 2025
Special Issue in
Biosensors
Advances in FET-Based Biosensors and Neuromorphic Devices: Exploring Artificial Perception and Biomimetic Sensing
Guest Editors: Yao Ni, Jiangdong GongDeadline: 28 February 2025
Special Issue in
Biosensors
Plasmonic Biosensors for Biomedical Applications
Guest Editors: Sónia O. Pereira, Cátia LeitãoDeadline: 28 February 2025
Topical Collections
Topical Collection in
Biosensors
Novel Sensing System for Biomedical Applications
Collection Editors: Chia-Ching Chang, Chiun-Jye Yuan, Chih-Chia Huang
Topical Collection in
Biosensors
Microsystems for Cell Cultures
Collection Editors: Iordania Constantinou, Thomas E. Winkler