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Prof. Shishmon Belkin 30th Anniversary of Working in Bioluminescent Bioreporter...
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Prof. Shishmon Belkin 30th Anniversary of Working in Bioluminescent Bioreporter Bacteria Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 2411

Special Issue Editors

Prof. Dr. Robert Steven Marks

E-Mail Website
Guest Editor
Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer-Sheva 8410500, Israel
Interests: biosensors; diagnostics; biomaterials; environmental toxicity; drug discovery; vaccines
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Kun Jia

E-Mail Website
Guest Editor
School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 611731, China
Interests: thin film sensor; immunsensor; raman; polymer materials
Dr. Evgeni Eltzov

E-Mail Website
Guest Editor
Department of Postharvest Science ARO, Institute of Postharvest and Food Sciences, The Volcani Center HaMaccabim Rd 68, POB 15159, Rishon LeZion 7528809, Israel
Interests: bioluminescent bacterial; point-of-care devices; environmental; nano-based biosensors; real-time monitoring systems
Dr. Boris Polyak

E-Mail Website
Guest Editor
Gold Standard Diagnostics Horsham, Horsham, PA 19044, USA
Interests: biological sensing; immunoassays; drug delivery

Special Issue Information

Dear Colleagues,

The Special Issue themed after retired Prof. Shishmon Belkin to honour his past accomplishments in the field will include various bodies of work such as biography, reviews, original research, target product profiles (new paper concept usually found in business plans), etc.… The topics covered are applications using bioluminescent bioreporter bacteria in screening or monitoring, adapted, or not, to a transducer configuration, thus tailored to be part of a biosensor system. Applications can be found in environmental toxicology, biotechnology engineering, information engineering (to understand complex mixtures), 3D bioinks, clinical applications, and more.

Prof. Dr. Robert Steven Marks
Prof. Dr. Kun Jia
Dr. Evgeni Eltzov
Dr. Boris Polyak
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioluminescence
  • bioreporters
  • biosensors
  • applications
  • commercialization

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Further information on MDPI's Special Issue polices can be found here.

Published Papers (2 papers)

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Research

21 pages, 13351 KiB  
Article
Bioluminescent Whole-Cell Bioreporter Bacterial Panel for Sustainable Screening and Discovery of Bioactive Compounds Derived from Mushrooms
by Calin Trif, Jovana Vunduk, Yardnapar Parcharoen, Aporn Bualuang and Robert S. Marks
Biosensors 2024, 14(11), 558; https://doi.org/10.3390/bios14110558 - 17 Nov 2024
Viewed by 506
Abstract
This study presents a rapid and comprehensive method for screening mushroom extracts for the putative discovery of bioactive molecules, including those exhibiting antimicrobial activity. This approach utilizes a panel of bioluminescent bacteria, whose light production is a sensitive indicator of various cellular effects [...] Read more.
This study presents a rapid and comprehensive method for screening mushroom extracts for the putative discovery of bioactive molecules, including those exhibiting antimicrobial activity. This approach utilizes a panel of bioluminescent bacteria, whose light production is a sensitive indicator of various cellular effects triggered by the extracts, including disruption of bacterial communication (quorum sensing), protein and DNA damage, fatty acid metabolism alterations, and oxidative stress induction. The bioassay’s strength is its ability to efficiently analyze a large number of extracts simultaneously while also assessing several different mechanisms of toxicity, significantly reducing screening time. All samples analyzed exhibited more than one cellular effect, as indicated by the reporter bacteria. Four samples (C. cornucopioides, F. fomentarius, I. obliquus, and M. giganteus) displayed the highest number (six) of possible mechanisms of antibacterial activity. Additionally, combining extraction and purification protocols with a bioluminescent bacterial panel enables simultaneous improvement of the desired antimicrobial properties of the extracts. The presented approach offers a valuable tool for uncovering the diverse antimicrobial mechanisms of mushroom extracts. Full article
(This article belongs to the Special Issue Prof. Shishmon Belkin 30th Anniversary of Working in Bioluminescent Bioreporter Bacteria Biosensors)
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22 pages, 8826 KiB  
Article
Microbead-Encapsulated Luminescent Bioreporter Screening of P. aeruginosa via Its Secreted Quorum-Sensing Molecules
by Abraham Abbey Paul, Yael Schlichter Kadosh, Ariel Kushmaro and Robert S. Marks
Biosensors 2024, 14(8), 383; https://doi.org/10.3390/bios14080383 - 8 Aug 2024
Viewed by 1582
Abstract
Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that remains a prevalent clinical and environmental challenge. Quorum-sensing (QS) molecules are effective biomarkers in pinpointing the presence of P. aeruginosa. This study aimed to develop a convenient-to-use, whole-cell biosensor using P. aeruginosa reporters individually [...] Read more.
Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that remains a prevalent clinical and environmental challenge. Quorum-sensing (QS) molecules are effective biomarkers in pinpointing the presence of P. aeruginosa. This study aimed to develop a convenient-to-use, whole-cell biosensor using P. aeruginosa reporters individually encapsulated within alginate-poly-L-lysine (alginate-PLL) microbeads to specifically detect the presence of bacterial autoinducers. The PLL-reinforced microbeads were prepared using a two-step method involving ionic cross-linking and subsequent coating with thin layers of PLL. The alginate-PLL beads showed good stability in the presence of a known cation scavenger (sodium citrate), which typically limits the widespread applications of calcium alginate. In media containing synthetic autoinducers—such as N-(3-oxo dodecanoyl) homoserine lactone (3-oxo-C12-HSL) and N-butanoyl-L-homoserine lactone (C4-HSL), or the cell-free supernatants of planktonic or the flow-cell biofilm effluent of wild P. aeruginosa (PAO1)—the encapsulated bacteria enabled a dose-dependent detection of the presence of these QS molecules. The prepared bioreporter beads remained stable during prolonged storage at 4 and −80 °C and were ready for on-the-spot sensing without the need for recovery. The proof-of-concept, optical fiber-based, and whole-cell biosensor developed here demonstrates the practicality of the encapsulated bioreporter for bacterial detection based on specific QS molecules. Full article
(This article belongs to the Special Issue Prof. Shishmon Belkin 30th Anniversary of Working in Bioluminescent Bioreporter Bacteria Biosensors)
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