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Biosensors
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Aptasensor for Biomarker Detection: From Design to Applications
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Aptasensor for Biomarker Detection: From Design to Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (20 March 2024) | Viewed by 5358

Special Issue Editor

Dr. Raja Chinnappan

E-Mail Website
Guest Editor
Department of Biochemistry & Molecular Medicine, Alfaisal University, Riyadh 11533, Saudi Arabia
Interests: portable point-of-care testing; biosensors; various diseases detection

Special Issue Information

Dear Colleagues,

The determination of specific biomarker proteins in complex biological fluids is essential for the diagnosis of certain diseases. Variations in the concentration of biomarkers are correlated with the infected diseases. Quantitative detection of biomarker proteins from the samples is crucial and challenging. It is common practice to use antibodies as bioreceptors for the quantification of proteins. Aptamers exhibit high affinity, and specificity and are sensitive to several biomarker proteins, which allow for the exploration of the probing of new biomarker proteins. The development of aptamer-based (aptasensor), simple, sensitive, and rapid diagnostic methods for the identification of new biomarkers would be of significant interest to researchers. Thus, this Special Issue ‘Aptasensor for Biomarker Detection: From Design to Applications’ would be an appropriate platform for you to share your recent research findings on aptasensors for biomarker diagnosis. Authors are invited to submit their manuscripts on the discovery of biomarkers through aprasensors and sensor design for the field of applications. Manuscripts with simple, low-cost mass production, high-throughput screening, and point-of-care testing of aptasensor design will be highly appreciated.

Dr. Raja Chinnappan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomarkers
  • aptamer
  • aptasensor
  • point-of-care testing
  • high-throughput screening
  • SELEX
  • ssDNA
  • bioreceptors
  • antibodies
  • biosensors

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Published Papers (2 papers)

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Research

13 pages, 1975 KiB  
Article
One Single Tube Reaction of Aptasensor-Based Magnetic Sensing System for Selective Fluorescent Detection of VEGF in Plasma
by Hwang-Shang Kou, Shao-Tsung Lo and Chun-Chi Wang
Biosensors 2023, 13(6), 574; https://doi.org/10.3390/bios13060574 - 24 May 2023
Cited by 1 | Viewed by 2359
Abstract
In this study, a simple, easy and convenient fluorescent sensing system for the detection of the vascular endothelial growth factor (VEGF) based on VEGF aptamers, aptamer-complementary fluorescence-labeled probe and streptavidin magnetic beads was developed in one single tube. The VEGF is the most [...] Read more.
In this study, a simple, easy and convenient fluorescent sensing system for the detection of the vascular endothelial growth factor (VEGF) based on VEGF aptamers, aptamer-complementary fluorescence-labeled probe and streptavidin magnetic beads was developed in one single tube. The VEGF is the most important biomarker in cancer, and it is investigated that the serum VEGF level varied according to the different types and courses of cancers. Hence, efficient quantification of VEGF is able to improve the accuracy of cancer diagnoses and the precision of disease surveillance. In this research, the VEGF aptamer was designed to be able to bind with the VEGF by forming G-quadruplex secondary structures; then, the magnetic beads would capture the non-binding aptamers due to non-steric interference; and finally, the fluorescence-labeled probes were hybridized with the aptamers captured by the magnetic beads. Therefore, the fluorescent intensity in the supernatant would specifically reflect the present VEGF. After an overall optimization, the optimal conditions for the detection of VEGF were as followed, KCl, 50 μM; pH 7.0; aptamer, 0.1 μM; and magnetic beads, 10 μL (4 μg/μL). The VEGF could be well quantified within a range of 0.2-2.0 ng/mL in plasma, and the calibration curve possessed a good linearity (y = 1.0391x + 0.5471, r = 0.998). The detection limit (LOD) was calculated to be 0.0445 ng/mL according to the formula (LOD = 3.3 × σ/S). The specificity of this method was also investigated under the appearance of many other serum proteins, and the data showed good specificity in this aptasensor-based magnetic sensing system. This strategy provided a simple, sensitive and selective biosensing platform for the detection of serum VEGF. Finally, it was expected that this detection technique can be used to promote more clinical applications. Full article
(This article belongs to the Special Issue Aptasensor for Biomarker Detection: From Design to Applications)
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22 pages, 4685 KiB  
Article
Carbon Nanofiber-Ionic Liquid Nanocomposite Modified Aptasensors Developed for Electrochemical Investigation of Interaction of Aptamer/Aptamer–Antisense Pair with Activated Protein C
by Meltem Maral and Arzum Erdem
Biosensors 2023, 13(4), 458; https://doi.org/10.3390/bios13040458 - 4 Apr 2023
Cited by 4 | Viewed by 2228
Abstract
Selective and sensitive detection of human activated protein C (APC) was performed herein by using carbon nanofiber (CNF) and ionic liquid (IL) composite modified pencil graphite electrode (PGE) and electrochemical impedance spectroscopy (EIS) technique. A carbon nanomaterial-based electrochemical aptasensor was designed and implemented [...] Read more.
Selective and sensitive detection of human activated protein C (APC) was performed herein by using carbon nanofiber (CNF) and ionic liquid (IL) composite modified pencil graphite electrode (PGE) and electrochemical impedance spectroscopy (EIS) technique. A carbon nanomaterial-based electrochemical aptasensor was designed and implemented for the first time in this study for the solution-phase interaction of DNA-Apt with its cognate protein APC as well as APC inhibitor aptamer–antidote pair. The applicability of this assay developed for the determination of APC in fetal bovine serum (FBS) and its selectivity against different proteins (protein C, thrombin, bovine serum albumin) was also examined. CNF-IL modified aptasensor specific to APC provided the detection limit as 0.23 μg/mL (equal to 3.83 nM) in buffer medium and 0.11 μg/mL (equal to 1.83 nM) in FBS. The duration of the proposed assay from the point of electrode modification to the detection of APC was completed within only 55 min. Full article
(This article belongs to the Special Issue Aptasensor for Biomarker Detection: From Design to Applications)
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