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Biosensors
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Advanced Label-Free Electrochemical Affinity Biosensors
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Advanced Label-Free Electrochemical Affinity Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 August 2022) | Viewed by 11632

Special Issue Editors

Prof. Dr. Ching-Chou Wu

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Guest Editor
Department of Bio-Industrial Mechatronics Engineering, National Chung Hsing University, 402 Taichung, Taiwan
Interests: integration of electrochemical microsensors and an electrokinetic microfluidic chip for biomedical and bio-industrial applications; immunosensing chip; DNA biosensing chip; cell-based chip; copper phosphate-based electrocatalytic chip
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Hung-Wei Yang

E-Mail Website
Guest Editor
Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan
Interests: biopolymer; nanobiomaterials; biosensing; microneedles; virus-like particles; drug delivery system; cell-based immunotherapy
Dr. Bor-Ran Li

E-Mail Website
Guest Editor
Institute of Biomedical Engineering, National Yang Ming Chiao Tung University, Room 468, Engineering Building VI, No. 1001 University Road, Hsinchu 30010, Taiwan
Interests: applied surface chemistry; nano-structure application; micro-fabrication; functional microfluidics; lab on a chip; in vitro diagnostic; point-of-care testing

Special Issue Information

Dear Colleagues,

The topic of this Special Issue is advanced label-free affinity biosensors based on electrochemical techniques, including potentiometry, amperometry, voltammetry, impedimetry, and capacitimetry. Label-free electrochemical biosensors have the advantages of reducing labeling and reacting procedures, saving considerable time in the detection process, efficiently equipping a portable potentiostat or an electrical analyzer to construct small diagnostic devices, and being suitable for large-scale production. Affinity biosensors, such as immunosensors, aptasensors, and genosensors, can specifically detect analytes in complex environments of biosamples using antibodies, aptamers, and probe DNA fragments. A successful label-free electrochemical affinity biosensor is necessary to reduce non-specific adsorption when detecting targets in a real sample. The emerging modalities, such as the directional immobilization of biorecognition elements, the design of engineered recombinant proteins, the nanostructured surface of electrodes, the collection of immunomagnetic beads, and the usage of high conductive nanoparticles, are worth exploring for increasing the sensing properties of biosensors. Integrating microfluidic control with the label-free electrochemical sensing device for accelerating analyte transportation and concentrating analyte on the electrode surface to promote immunoreacting efficiency is welcomed in this issue. The concerned application fields of electrochemical affinity biosensors include medical diagnostics, point-of-care testing, food safety, and environmental pollution. For that, this Special Issue aims to collect original articles and reviews showing advanced research, fabrication, innovative applications, new challenges, and future perspectives of label-free electrochemistry-based biosensors in different essential areas.

Prof. Dr. Ching-Chou Wu
Prof. Dr. Hung-Wei Yang
Dr. Bor-Ran Li
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrochemistry
  • nanowire FET
  • immunosensor
  • aptasensors
  • genosensors
  • label free
  • nanozyme
  • lab-on-a-chip
  • point-of-care
  • microfluidics

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Published Papers (3 papers)

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Research

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12 pages, 9174 KiB  
Article
A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins
by Huilin Gao, Yongchang Bai, Baixun He and Cherie S. Tan
Biosensors 2022, 12(12), 1180; https://doi.org/10.3390/bios12121180 - 18 Dec 2022
Cited by 10 | Viewed by 3229
Abstract
The level of C-reactive protein (CRP) in the human body is closely associated with cardiovascular diseases and inflammation. In this study, a label-free functionalized aptamer sensor was attached to an electrode trimmed with in-gold nanoparticles and carboxylated graphene oxide (AuNPs/GO-COOH) to achieve sensitive [...] Read more.
The level of C-reactive protein (CRP) in the human body is closely associated with cardiovascular diseases and inflammation. In this study, a label-free functionalized aptamer sensor was attached to an electrode trimmed with in-gold nanoparticles and carboxylated graphene oxide (AuNPs/GO-COOH) to achieve sensitive measurements relative to CRP. Gold nanoparticles were selected for this study due to super stability, remarkably high electrical conductivity, and biocompatibility. In addition, carboxylated graphene oxide was utilized to promote the anchorage of inducer molecules and to increase detection accuracies. The sensing signal was recorded using differential pulse voltammetry (DPV), and it produced a conspicuous peak current obtained at approximately −0.4 V. Furthermore, the adapted sensor manifested a broad linear span from 0.001 ng/mL to 100 ng/mL. The results also demonstrated that this aptamer sensor had superior stability, specificity, and reproducibility. This aptamer-based electrochemical sensor has enormous potential in complex application situations with interfering substances. Full article
(This article belongs to the Special Issue Advanced Label-Free Electrochemical Affinity Biosensors)
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13 pages, 2624 KiB  
Article
The Characterization of Binding between Aptamer and Bisphenol A and Developing Electrochemical Aptasensors for Bisphenol A with Rationally Engineered Aptamers
by Liying Liu, Hao Yu and Qiang Zhao
Biosensors 2022, 12(11), 913; https://doi.org/10.3390/bios12110913 - 23 Oct 2022
Cited by 3 | Viewed by 2544
Abstract
Bisphenol A (BPA) is widely used in the manufacture of polycarbonate and epoxy-resin-based products, and BPA contamination often happens in a variety of types of environment and food stuffs. BPA can cause many harmful effects to health due to its high toxicity. The [...] Read more.
Bisphenol A (BPA) is widely used in the manufacture of polycarbonate and epoxy-resin-based products, and BPA contamination often happens in a variety of types of environment and food stuffs. BPA can cause many harmful effects to health due to its high toxicity. The rapid detection of BPA is of great significance in environmental monitoring and food safety. Nucleic acid aptamers show advantages in biosensors due to good chemical stability, the ease of labeling functional groups, and target binding that induces conformation change. Here, we performed a thorough characterization of the binding performance of one 60-nt anti-BPA DNA aptamer with isothermal titration calorimetry (ITC). We found the crucial region of the aptamer sequence for affinity binding with BPA, and the aptamer was able to be truncated to 29-nt DNA without losing affinity. We then developed a simple reagent-less electrochemical aptamer-based sensor for rapid BPA detection with this engineered aptamer. The truncated aptamer with a redox tag methylene blue (MB) was immobilized on a gold electrode. BPA-binding induced the conformation change of the MB-labeled aptamer, moving the MB close to the electrode surface and causing a significant current increase in MB in square wave voltammetry (SWV). Under optimized conditions, we achieved the quantitative detection of BPA with a detection limit of BPA at 0.1 μM. This sensor showed quick response to BPA and could be regenerated by washing with deionized water. This sensor was selective, and it allowed detecting BPA in complex samples, showing its potential in practice. This study will help in further applications of the aptamers of BPA. Full article
(This article belongs to the Special Issue Advanced Label-Free Electrochemical Affinity Biosensors)
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Review

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24 pages, 4667 KiB  
Review
Recent Advances in Electrochemical Immunosensors with Nanomaterial Assistance for Signal Amplification
by Avinash V. Police Patil, Yu-Sheng Chuang, Chenzhong Li and Ching-Chou Wu
Biosensors 2023, 13(1), 125; https://doi.org/10.3390/bios13010125 - 11 Jan 2023
Cited by 40 | Viewed by 5229
Abstract
Electrochemical immunosensors have attracted immense attention due to the ease of mass electrode production and the high compatibility of the miniature electric reader, which is beneficial for developing point-of-care diagnostic devices. Electrochemical immunosensors can be divided into label-free and label-based sensing strategies equipped [...] Read more.
Electrochemical immunosensors have attracted immense attention due to the ease of mass electrode production and the high compatibility of the miniature electric reader, which is beneficial for developing point-of-care diagnostic devices. Electrochemical immunosensors can be divided into label-free and label-based sensing strategies equipped with potentiometric, amperometric, voltammetric, or impedimetric detectors. Emerging nanomaterials are frequently used on electrochemical immunosensors as a highly rough and conductive interface of the electrodes or on nanocarriers of immobilizing capture antibodies, electroactive mediators, or catalyzers. Adopting nanomaterials can increase immunosensor characteristics with lower detection limits and better sensitivity. Recent research has shown innovative immobilization procedures of nanomaterials which meet the requirements of different electrochemical immunosensors. This review discusses the past five years of advances in nanomaterials (metal nanoparticles, metal nanostructures, carbon nanotubes, and graphene) integrated into the electrochemical immunosensor. Furthermore, the new tendency and endeavors of nanomaterial-based electrochemical immunosensors are discussed. Full article
(This article belongs to the Special Issue Advanced Label-Free Electrochemical Affinity Biosensors)
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